Technical and theoretical considerations in the HLA typing of amniotic fluid cells for prenatal diagnosis and paternity testing

HLA typing of amniotic fluid cells has been used for the prenatal diagnosis of the HLA linked diseases congenital adrenal hyperplasia (21-OH-deficiency (21-OH-def) type) and complement C4 deficiency and it has also been used for the prenatal de termination of paternity. There are, however, technical difficulties in this test associated with the weak expression of some B locus antigens on amniotic fluid cells, and theoretical difficulties related to associations between particular HLA antigens and the 21-OH-def allele. Since certain HLA-B locus antigens are found in significantly increased frequencies among patients with 21-OH-def, there is a relatively high incidence of HLA-B homozygosity among the patients and over 40 percent of the parents of these patients share one or more HLA-B locus antigens. Results of some prenatal HLA typing tests may thus be difficult to interpret, and supplementary tests should be used whenever possible. HLA typing of amniotic cells is, however, the only available procedure for prenatal diagnosis of C4 deficiency and it is the best available procedure for prenatal determination of paternity. A modification of our original procedure allows HLA typing to be performed with increased numbers of HLA typing sera, and sera with optimum reactivity for amniotic fluid cells have now been selected for the definition of most of the more commonly expressed HLA antigens. Although amniotic fluid cells do not express DR antigens, amniotic fluid cells can be typed for the HLA-linked marker glyoxalase I (GLO) and this may be the informative for prenatal diagnosis in some cases.     

Study of the future land use of a contaminated site: Preferences versus potential use

Worldwide, agencies with high levels of contamination are faced with decisions about remediation and restoration. These decisions should be informed by future land use and long‐term stewardship goals. In the United States, the Department of Energy has lands in some 34 states that require cleanup. They are involved in massive remediation and restoration efforts on lands from the Cold War legacy and wish to reduce their overall footprint. Understanding future land use preferences is essential for determining the nature and degree of remediation and restoration. The objective of this study was to examine future land use preferences for the Department of Energy's Los Alamos National Laboratory as a function of ethnicity for attendees at the Los Alamos Gun Show in New Mexico (1999), and to determine whether their own activity influences future land use preferences. The highest preferred future land uses for Los Alamos National Laboratory were hiking, camping, National Environmental Research Park, and birdwatching, followed by hunting and fishing. Increased nuclear waste storage and building homes and factories were rated the lowest. Further, hiking and camping were rated higher than at two other DOE sites. There were few ethnic differences, although American Indians rated camping, hiking, building houses, and returning the land to American Indians higher than did others, and Hispanics rated using it for a preserve as a higher preferred land use than all others. The differences, however, were not great. Relative ratings for using the land for hunting and fishing were directly related to individual frequency of hunting and fishing for both whites and Hispanics, indicating that people perceive the importance of land use by how they want to use it. Ratings for hiking and camping were not related to the number of days people hiked and camped, suggesting these are general preferences overall. © 2004 Wiley Periodicals, Inc.     

End‐state land uses,sustainably protective systems,and risk management: A challenge for remediation and multigenerational stewardship

This article discusses creating a sustainably protective engineered and human management system in perpetuity for sites with long‐lived radiological and chemical hazards. This is essential at this time because the federal government is evaluating its property as assets and attempting to reduce its holdings, while seeking to assure that health and ecosystems are not put at risk. To assist those who have a stake in the remediation, management, and stewardship of these and analogous privately owned sites, this article discusses current end‐state planning by reviewing the federal government's accelerated efforts to reduce its footprint and how those efforts relate to sustainability. The article also provides a list of questions organized around six elements of risk management and primary, secondary, and tertiary disease and injury prevention. Throughout the article, the U.S. Department of Energy (DOE) is used as an example of an organization that seeks to reduce its footprint, manage its budget, and be a steward of the sites that it is responsible for. However, the approach and questions are appropriate for land controlled by the Department of Defense (DOD), the General Services Administration (GSA), and other public and private owners of sites with residual contamination. © 2005 Wiley Periodicals, Inc.     

Variation in root density along stream banks

While it is recognized that vegetation plays a significant role in stream bank stabilization, the effects are not fully quantified. The study goal was to determine the type and density of vegetation that provides the greatest protection against stream bank erosion by determining the density of roots in stream banks. To quantify the density of roots along alluvial stream banks, 25 field sites in the Appalachian Mountains were sampled. The riparian buffers varied from short turfgrass to mature riparian forests, representing a range of vegetation types. Root length density (RLD) with depth and aboveground vegetation density were measured. The sites were divided into forested and herbaceous groups and differences in root density were evaluated. At the herbaceous sites, very fine roots (diameter < 0.5 mm) were most common and more than 75% of all roots were concentrated in the upper 30 cm of the stream bank. Under forested vegetation, fine roots (0.5 mm < diameter < 2.0 mm) were more common throughout the bank profile, with 55% of all roots in the top 30 cm. In the top 30 cm of the bank, herbaceous sites had significantly greater overall RLD than forested sites (alpha = 0.01). While there were no significant differences in total RLD below 30 cm, forested sites had significantly greater concentrations of fine roots, as compared with herbaceous sites (alpha = 0.01). As research has shown that erosion resistance has a direct relationship with fine root density, forested vegetation may provide better protection against stream bank erosion.     

A framework for analysis of contamination on human and ecological receptors at DOE hazardous waste site buffer lands

This article discusses a framework and tools for evaluating ecological resources and the effects of cleanup on hazardous waste sites, particularly those with ecological buffer lands. Environmental professionals are faced with assessing the risks of contamination to humans and ecological receptors (organisms and ecosystems) at hazardous waste sites. While exposure assessment has focused largely on human receptors, environmental managers have recently taken a broader view, recognizing the intrinsic value and aesthetic importance of ecological resources and services, and of including a range of stakeholders in remediation decisions. The assessment process involves understanding exposure pathways from source to receptor, and determining how best to interdict these pathways. Environmental characterization and exposure assessment, indicator and biomarker identification, and biomonitoring and surveillance are the major components of ecological assessments. Using the Department of Energy as a case study, this article offers a framework for ecological exposure assessment, recognizing that humans are important components of ecosystems and, like other biota, are exposed to contaminants that move through environmental media. © 2007 Wiley Periodicals, Inc.     

Relictual Amphibians and Old-Growth Forests

Abstract: Terrestrial and aquatic herpetofauna were sampled by pitfall traps, time-constrained searches, and area-constrained searches (stream sites only) over a three-year period to examine the importance of forest age to amphibians and reptiles. Fifty-four terrestrial and 39 aquatic sites in Douglas-fir—dominated, mixed evergreen forests were located in southwestern Oregon and northwestern California Mean age of trees on sites ranged in age from 30 to 560 years. Thirty-one species of amphibians and reptiles were detected from the 93 localities. Only three species were found primarily on older forest sites: the Del Norte salamander (Plethodon elongatus), the Olympic salamander (Rhyacotriton olympicus), and the tailed frog (Ascaphus truei). Paleoeco-logic evidence indicates an historical association between these three amphibians and the extant elements of ancient primeval coniferous forests of the Pacific Northwest. The life histories and habitat requirements of these species suggest that these forms are scarce in younger forests because the microclimatic and microhabitat conditions they require generally exist only in older forests. The long-term viability of these species in northern California and southern Oregon may depend upon developing forestry practices that protect these mitical microclimates and microhabitats.     

Integrating long-term stewardship goals into the remediation process: natural resource damages and the Department of Energy

The United States and other developed countries are faced with restoring and managing degraded ecosystems. Evaluations of the degradation of ecological resources can be used for determining ecological risk, making remediation or restoration decisions, aiding stakeholders with future land use decisions, and assessing natural resource damages. Department of Energy (DOE) lands provide a useful case study for examining degradation of ecological resources in light of past or present land uses and natural resource damage assessment (NRDA). We suggest that past site history should be incorporated into the cleanup and restoration phase to reduce the ultimate NRDA costs, and hasten resource recovery. The lands that DOE purchased over 50 years ago ranged from relatively undisturbed to heavily impacted farmland, and the impact that occurred from DOE occupation varies from regeneration of natural ecosystems (benefits) to increased exposure to several stressors (negative effects). During the time of the DOE releases, other changes occurred on the lands, including recovery from the disturbance effects of farming, grazing, and residential occupation, and the cessation of human disturbance. Thus, the injury to natural resources that occurred as a result of chemical and radiological releases occurred on top of recovery of already degraded systems. Both spatial (size and dispersion of patch types) and temporal (past/present/future land use and ecological condition) components are critical aspects of resource evaluation, restoration, and NRDA. For many DOE sites, integrating natural resource restoration with remediation to reduce or eliminate the need for NRDA could be a win-win situation for both responsible parties and natural resource trustees by eliminating costly NRDAs by both sides, and by restoring natural resources to a level that satisfies the trustees, while being cost-effective for the responsible parties. It requires integration of remediation, restoration, and end-state planning to a greater degree than is currently done at most DOE sites.     

Ripping Improves Tree Survival and Growth on Unused Reclaimed Mined Lands

There is renewed interest in re-establishing trees on 0.6 million ha of mining-disturbed lands in the Appalachian mountains of Eastern United States. Many coal-mined lands reclaimed to meet requirements of US federal law have thick herbaceous vegetation and compacted soils which impede tree establishment. Mitigation practices were applied on three mine sites and evaluated for success in enabling planted trees to become established. Eastern white pine (Pinus strobus), hybrid poplar (Populus deltoids × Populus trichocarpa), and mixed Appalachian hardwoods were established using weed control only and weed control with subsoil ripping. Trees were measured in October of 2008 after 5 years of growth. Subsoil ripping increased mixed hardwood survival from 43 to 71 %, hybrid poplar biomass index from 1.51 to 8.97 Mg ha^?1, and Eastern white pine biomass index from 0.10 to 0.32 Mg ha^?1. When restoring trees to unused mined sites, subsoil ripping can aid survival and growth to an extent that will result in a valuable forest.     

Founding Lineages and Genic Variability in Plains Bison (Bison bison) from Badlands National Park, South Dakota

Abstract: Starch-gel electrophoresis was used to screen 101 bison from Badlands National Park, South Dakota, for variation at 24 genetic loci. The population was descended from founder groups of about 6 and 3 individuals, separated geographically for a minimum of 64 years. The purpose of this study was (1) to estimate levels of genic variability in this bison population, (2) to assess the extent to which descendents of the two founder groups differ genetically, and (3) to compare the genetic characteristics of the Badlands population with other bison populations. The Badlands herd was found to be polymorphic for only a single locus (MDH–1). Descendents of the founder groups were homogeneous with respect to allelic and genotypic frequencies at this locus. The MDH–1 polymorphism has not been observed in other bison populations, while several polymorphism reported in other bison populations were not detected in the Badlands herd. A mean heterozygosity of 0.012 was observed in the Badlands herd; this value is lower than that typically reported for mammals, though not as low as heterozygosities seen in other populations that have passed through severe bottlenecks in size. These results underscore the need for genetic data in planning breeding programs for species in captivity or managed in isolate reserves.     

Lands for Long-Term Research in Conservation Biology

Abstract: Success in conservation biology depends upon a synergistic combination of short-term tactics and long-term strategies. Although the former are often necessary to forestall immediate habitat losses, the latter provide the critical framework of understanding needed to develop effective short-term priorities. Although many conservation biologists now emphasize short-term tactics, prudence dictates the concomitant establishment of long-term research projects aimed at answering fundamental ecological questions.
One deterrent to amassing such long-term data bases, aside from time and funding, is a lack of suitable sites where such studies can be conducted on a large scale. We describe two established major land-holding networks in the United States that could serve as appropriate places to develop long-term studies: the National Science Foundation's Long-Term Ecological Research (LTER) sites, and the US. Department of Energy's National Environmental Research Parks (NERPs). Because they consist of established research facilities, both networks can provide conservation biologists with "low-cost" baseline information on ecological processes, as well as access to a number of representative terrestrial and aquatic habitats under more or less "controlled" conditions suitable for long-term studies. We recommend that conservation biologists explore the possibility of using these or similarly available sites in their research programs.