基于螺旋相位调制的非相干全息点扩散函数研究

分析了菲涅耳非相干相关全息(Fresnel incoherent correlation holography,FINCH)系统中纯相位空间光调制器(spatial light modulator,SLM)加载螺旋相位掩模时的点扩散函数.以氙灯为照明光源搭建了FINCH系统,电荷耦合器记录的点源全息图与点扩散函数模拟结果一致.采用该系统分别在SLM上加载双透镜掩模和螺旋相位调制双透镜掩模两种情况下对分辨率板和非染色洋葱细胞成像,给出了成像对比结果.结果表明:采用螺旋相位调制的FINCH系统可以在几乎不牺牲分辨率的情况下提高图像的边缘对比度;同样,对相位物体也可以实现图像的边缘提取和识别.该方法在实时监测活细胞的分裂、形变等方面具有重要应用前景.     

菲涅耳非相干数字全息大视场研究

菲涅尔非相干相关全息术(Fresnel incoherent correlation holography, FINCH)通过空间光调制器(spatial light modulator, SLM)将来自物点的光波分解为曲率半径不同的两束自相干光,干涉条纹由CCD记录.由于受限于SLM与CCD的像素数目及像素尺寸, FINCH技术与光学全息术相比记录视场要小得多.本文通过对FINCH系统的记录过程进行理论分析,给出了SLM所能记录的视场角,说明通过调控加载在SLM上的双透镜光轴中心,能够扩大SLM的有效直径从而将SLM的有效记录范围增大2.77倍,有效扩大了系统的记录视场.搭建了非相干光反射式数字全息记录系统并对理论分析进行了实验验证,结果表明:在SLM上依次加载不同光轴中心位置的双透镜掩模进行FINCH记录及再现,将得到的各子图像拼接融合可以得到高分辨率大视场图像,为菲涅尔非相干全息术在高分辨大视场显微成像的进一步应用提供了有力支撑.     

图像传感器像素化效应对菲涅耳非相干关联全息分辨率的影响

作为复光场显微成像的一种新技术,菲涅耳非相干关联全息术(Fresnel incoherent correlation holography,FINCH)因其非相干光记录的特点在近年来受到关注.FINCH作为一种新型非相干全息系统,如何设计光路实现其最佳的分辨率是一个关键问题.然而,针对这个问题的讨论,目前已有文献存在不同的观点,有关FINCH最佳分辨率的成像条件仍有待研究.全息图有效孔径大小是决定全息成像系统分辨率的重要因素,在FINCH系统中,全息记录距离的变化则会引起全息图有效孔径发生变化,全息图的有效孔径大小不仅与光路各元件的孔径有关,还与相干光波相互干涉叠加区域的面积以及图像传感器的像素间距等因素有关.本文基于波动光学理论,结合FINCH全息图的波带结构特征,研究了FINCH全息图的有效孔径.研究发现数字全息记录相机的像素化特性是影响FINCH成像分辨率的决定性因素,并进一步通过数值模拟和光学实验验证了理论分析结果:全息图记录距离(Z_h)等于空间光调制器加载的衍射透镜焦距(f_d)时,FINCH系统的再现像将会达到最佳横向分辨率,且分辨率随成像距离|Z_h-f_d|的增大而降低.     

提高数字全息再现像像质的两步相移迭代算法

在两步相移数字全息术中,相移误差直接影响着零级像和共轭像的消除.为了消除相移误差,获得实际相移值,提出了一种基于相位统计特性的两步相移迭代算法.首先利用全息图的相位统计特性计算出初始相移值,然后用数字全息图再现像抽样点强度偏差之和作为评价标准,通过迭代计算寻找参考光的真正相移值.该算法能对任意未知相移量进行提取,从而有效地消除零级像和共轭像,提高了再现像质量.理论分析和光学实验结果证明了该方法的可行性和有效性.     

相移数字全息子图拼接研究

 将全息图拼接技术应用到相移数字全息术中,改善再现像的质量。用全息图强度相关原理进行全息图拼接,再通过标准四步算法对拼接后的全息图进行运算,恢复原始物光。作为对比,对没有拼接的全息图进行了同样的操作。计算机模拟实验证明该全息图拼接技术可明显提高再现像的质量。同时,通过对光学实验获得的全息图进行零填充和拼接对比研究证明,前者只能提高系统成像的细节显示能力,后者能够提高再现像的分辨率和视场大小。     

基于位相变更的非相干数字全息自适应成像

菲涅耳非相干数字全息作为一种非扫描的三维成像技术具有其独特的优势,但其成像过程中会受到各种像差的影响,导致成像分辨率、再现像的质量降低.为了解决这一问题,可以结合适当的自适应光学技术对波前像差进行探测和校正.位相变更是一种基于两幅具有已知位相差的强度图像实现波前探测和像差校正的技术.本文发展了基于位相变更的非相干数字全息自适应成像技术,不需要引入引导星,利用全息记录过程中的两幅相移全息图,实现波前像差的探测.本文给出了所发展技术的数值仿真和实验结果,结合位相变更算法求解出系统像差的位相分布,将像差的共轭位相加载到光瞳面上,在全息图记录的同时校正像差,从而提高重建像的质量.     

基于像素结构空间光调制器的全息再现像问题研究

基于纯相位空间光调制器的全息显示系统在重构显示时,再现像的视觉效果受到空间光调制器像素结构引起的多级衍射光和多级再现像的干扰。在分析具有有限填充因子空间光调制器的像素结构对再现像影响的基础上,提出了一种提高全息再现像的视觉效果并且再现像成像位置和大小可调节的方法。先加载闪耀光栅到纯相位全息图,其次通过叠加会聚球面波相位,分离再现像与空间光调制器像素结构引起的多级衍射光的聚焦平面的位置,再利用光阑和高通滤波器的共同作用,消除高级衍射光、多级再现像以及零级光干扰对重构视觉效果的影响,最后引入成像透镜,调节再现像的成像位置与大小。建立了一套基于硅基液晶的全息显示系统用于实验验证。实验结果表明,最终的单一再现像清晰且可以方便地调节成像位置和大小。该方法同样适用于各种基于像素结构空间光调制器的全息光学系统。     

基于两步正交相移干涉的振幅图像光学加密技术

提出一种基于两步正交相移干涉的光学图像加密技术.这种相移干涉数字全息只要记录两幅干涉图,不需要记录物光波和参考光波的强度信息,就可以再现没有零级像和共轭像的再现像.物光波对应的光路经过两次菲涅尔变换,并结合双随机相位编码.参考光分别引入0和π/2相位,用数字化记录介质记录两幅数字全息图作为加密图像.解密时只要获得正确的密钥,经过简单的计算就可以重建清晰的原始图像.模拟实验验证了它的可行性和有效性,分析了抗裁剪和噪音的鲁棒性.     

内源全息术的原理和数学描述

以往的全息术在对原子成像时,在相干光源、探测器空间分辨和信号强度三个方面遇到了难以克服的困难. 阐述了内源全息术克服这些困难的原理,研究了内源全息术的记录和再现全过程,推导出内源球面全息图的再现公式,并在散射因子球对称近似和能量无关近似下,推导出以δ函数表示原子全息再现像的解析表达式. 关键词： 内源全息术 同步辐射 晶体结构 傅里叶变换     

基于光程差扫描的低相干离轴数字全息术

针对在低相干离轴数字全息术中,光源相干长度限制物光波可探测面积的问题,提出了一种基于光程差扫描的低相干离轴数字全息记录方法.首先,通过改变参考光和物光波之间的光程差,使得干涉条纹扫描记录平面的不同区域,而后对各个区域的数字全息图采用数值再现的方法获取不同区域处的物光波相位分布,最后采用相位拼接技术拼接各个区域的相位分布实现物光波的全视场探测.     

Incoherent digital holographic spectral imaging with high accuracy of image pixel registration

Fresnel incoherent correlation holography(FINCH) is a unique three-dimensional(3 D) imaging technique which has the advantages of scanning-free,high resolution,and easy matching with existing mature optical systems.In this article,an incoherent digital holographic spectral imaging method with high accuracy of spectral reconstruction based on liquid crystal tunable filter(LCTF) and FINCH is proposed.Using the programmable characteristics of spatial light modulator(SLM),a series of phase masks,none of whose focal lengths changes with wavelength,is designed and made.For each wavelength of LCTF output,SLM calls three phase masks with different phase constants at the corresponding wavelength,and CCD records three holograms.The spectral images obtained by this method have a constant magnification,which can achieve pixel-level image registration,restrain image registration errors,and improve spectral reconstruction accuracy.The results show that this method can not only obtain the 3 D spatial information and spectral information of the object simultaneously,but also have high accuracy of spectral reconstruction and excellent color reproducibility.     

Review of Fresnel incoherent correlation holography with linear and non-linear correlations

Fresnel incoherent correlation holography(FINCH) is a well-established incoherent imaging technique. In FINCH, three selfinterference holograms are recorded with calculated phase differences between the two interfering, differently modulated object waves and projected into a complex hologram. The object is reconstructed without the twin image and bias terms by a numerical Fresnel back propagation of the complex hologram. A modified approach to implement FINCH by a single camera shot by pre-calibrating the system involving recording of the point spread function library and reconstruction by a nonlinear cross correlation has been introduced recently. The expression of the imaging characteristics from the modulation functions in original FINCH and the modified approach by pre-calibration in spatial and polarization multiplexing schemes are reviewed. The study reveals that a reconstructing function completely independent of the function of the phase mask is required for the faithful expression of the characteristics of the modulating function in image reconstruction. In the polarization multiplexing method by non-linear cross correlation, a partial expression was observed, while in the spatial multiplexing method by non-linear cross correlation, the imaging characteristics converged towards a uniform behavior.     

Joint approach of the sub-holograms in on-axis lensless Fourier phase-shifting synthetic aperture digital holography

In on-axis lensless Fourier phase-shifting synthetic aperture digital holography, to compose all of the phase-shifting sub-holograms to a large synthetic aperture digital hologram effectively, firstly, the cross-correlation algorithm of the object waves is presented to correct the joint misplacement of the sub-holograms. Secondly, to make the phase-shifting synchronization matching of different sequence phase-shifting holograms, the cross-correlation algorithm of the phase-shifting holograms is employed. Compared with the traditional cross-correlation algorithm of the sub-holograms, the proposed approach makes the joint precision of the sub-holograms reach sub-pixel accuracy, and the resolution of the reconstructed image is improved significantly. In general, the proposed approach is effective in restraining the quality degradation of the synthetic reconstructed image that comes from the joint misplacement of the sub-holograms and the phase-shifting non-synchronization of the phase-shifting holograms.     

Studies on aperture synthesis in digital Fresnel holography

Aperture synthesis can improve image resolution in digital holography by increasing the numerical aperture of the system. In this paper, we show that both the lateral resolution and image field of view can be enhanced at the same time using a more general Fresnel holography setup and hologram stitching. The impact of aperture synthesis on the lateral resolution is investigated both theoretically and experimentally. In the experiment, the synthesis is executed by moving the compact digital holographic system in two directions. Nine holograms are recorded and stitched into one hologram. The reconstruction results show that expanding aperture improves the lateral resolution. The lensless Fresnel holography used in this paper is demonstrated to have the ability to provide a larger numerical aperture and can compress the object spectrum in recording process.     

菲涅尔数字全息的脉冲响应

数字全息是用CCD记录全息图并用计算机数值重建全息像的一种全息新方法.在数字全息中,通过对不同记录参数下记录的全息图的数值处理,可以消除零级光和共轭光,从而将数字全息系统看作是一个线性系统.本文依据全息理论和付里叶频谱分析,对菲涅尔数字全息系统的脉冲响应和分辨本领进行了理论分析.结果表明,在矩形等间隔抽样的情形下,菲涅尔数字全息的脉冲响应是由CCD有限大小的孔径衍射斑调制的矩形函数;菲涅尔数字全息的分辨率由CCD的孔径尺寸决定;由于CCD像素具有一定的大小,使得点光源的像发生弥散.