Lipid binding of formula bread doughs Relationships with dough and bread technological performance

 Lipid binding of straight/soured started bread doughs treated with sodium carboxymethylcellulose (CMC), hydroxypropylmethylcellulose (HPMC), fungal α-amylase and monoglycerides (MGL), diacetyl tartaric acid esters of mono-diglycerides (DATEM) and sodium stearoyl lactylate (SSL) was investigated and results correlated with dough and bread performance during breadmaking and storage. For doughs formulated with MGL or DATEM, free and bound lipids accounted, respectively, for 70% and 30% of the increase in non-starchy lipids, which preferentially bind to gluten (MGL) and to the outside part of the starch granules (DATEM). SSL mainly increased the pool of free lipids and preferentially bound to the inside part of the starch granules and loosely to the gluten. Hydrocolloids preferentially bound to the gluten (CMC) and to the outside part of the starch granules (HPMC) respectively; this was associated with a significant displacement of endogenous gluten-bounded lipids to the starchy fraction (CMC) and with a significant decrease in lipids bound to the outside part of the starch granules (HPMC). The addition of α-amylase promoted a release of endogenous, bound lipids, and the sourer starter induced the aggregation of the starch-lipid complexes, revealed by the respective decrease in the level of gluten bounded lipids (α-amylase) and increase in the level of starchy lipids. Desired trends in dough lipid parameters resulting in strengthened gluten, delayed starch gelatinization, softer bread and reduced/delayed bread staling corresponded to high values of both free and starchy lipids, achieved by the incorporation of SSL and/or CMC into doughs. Received: 22 January 1998     

Antistaling Additives, Flour Type and Sourdough Process Effects on Functionality of Wheat Doughs

Antistaling additives—distilled monoglycerides (MGL), diacetyl tartaric ester of monodiglycerides (DATEM), sodium stearoyl lactylate (SSL), carboxymethylcellulose (CMC), hydroxypropylmethylcellulose (HPMC) and fungal α-amylase—were studied for effects on rheological and fer- mentative properties of white/whole wheat bread doughs, made following straight/sour dough processes. A fractionated factorial design (L³²) was used to evaluate single additive effects and interactions. Single addition of DATEM, followed by SSL, α-amylase and hydrocolloids improved oven rise and final volume. In presence of DATEM, synergistic (MGL) and antagonistic (SSL) effects of additional emulsifiers were found on gassing power. SSL was the only effective conditioner for enhancement of mixing properties. Dough plasticity was negatively affected by MGL addition and by CMC/HPMC in white/whole flours respectively. Some combinations resulted in detrimental dough handling properties.     

Effect of damaged starch levels on flour-thermal behaviour and bread staling

The effect of the amount of damaged starch in two different flours (wheat and triticale) on the bread quality and its behaviour during storage has been analysed. Two wheat and one triticale flour cultivars were milled in a disc mill to obtain different levels of damaged starch. Differential Scanning Calorimetry (DSC) and Rapid Visco Analyser (RVA) were used to characterize the flour properties and TA-XT2 textural analyses were made on breadcrumb. The effect of the damaged starch content on the bread firming, the amylopectin retrogradation and starch-pasting properties were studied in order to establish any relationship between damaged starch and bread staling. DSC analysis showed that the damaged starch content changed the thermal behaviour of flour–water mixtures: the higher the levels of damaged starch the lower the starch-gelatinization enthalpy and the higher the melting enthalpy of amylose–lipid complexes. The amount of amylopectin retrogradation and breadcrumb firming increased with the damaged starch content at the beginning of storage time; however, differences were decreasing at final storage time. The flour viscosity during pasting decreased as their damaged starch content increased.     

INFLUENCE OF GUMS ON DOUGH PROPERTIES AND FLAT BREAD QUALITY OF TWO PERSIAN WHEAT VARIETIES

The effects of separate mixing of two hydrocolloids carboxymethylcellulose (CMC) and hydroxypropylmethylcellulose (HPMC) at 0.1, 0.3 and 0.5% levels with Sardary and Sorkheh wheat flours (two native Persian varieties) were studied. Chemical and rheological (Farinograph and Extensograph) tests, staling and sensory evaluations were performed on the two flours, their dough and the resulting Lavash flat bread, respectively. On the basis of split‐plot experiment in a complete random design and using the Duncan's multiple range tests, the data were evaluated and the average of replicates was compared at the statistical level of 1% (α = 0.01). Although the CMC and HPMC gums enhanced significantly the dough quality of the two wheat varieties by increasing water absorption and reducing resistances after 90 and 135 min (compared with control), the effect of HPMC was more pronounced than CMC. Anti‐staling properties of HPMC were better than CMC, especially for Sardary flour. Separate addition of 0.5% CMC and HPMC gums to Sorkheh and Sardary flours significantly delayed the staling process of the resulting Lavash bread by more than 45 and 42%, respectively. Additionally, sensory evaluation scores of the Lavash bread made from either Sorkheh flour containing 0.3% CMC or HPMC or Sardary flour containing 0.5% HPMC were 50 and 120% higher than control bread samples, respectively.     

Improvement of the breadmaking quality of wheat flour by the hyperthermophilic xylanase B from Thermotoga maritima

The study was carried out to investigate the effect of hyperthermophilic xylanase B (XynB) from Thermotoga maritima on the properties of wheat bread and its staling during storage. The presence of XynB in the dough led to improvements in the breadmaking quality (i.e. specific volume and crumb structure) and provided an anti-staling effect on breads compared to the control. Addition of XynB could cause ≈60% increase in specific volume in comparison with the control. By fitting the crumb firming kinetics during storage to the Avrami equation, it showed that XynB retarded the bread staling by reducing the initial crumb firmness and the firming process during storage. XynB hydrolyzed the isolated WU-AX faster than WE-AX under the ratio of wheat AX in wheat flour. Improvement of bread quality by XynB can partly be ascribed to the enzyme specificity.     

Impact of microbial transglutaminase on the staling behaviour of enzyme-supplemented pan breads

The effects of microbial transglutaminase (TGM) when added singly and in combination with amylolytic (-amylase, NMYL) and non-amylolytic (xylanase) enzymes on the textural profile of fresh pan beads and pan breads stored for up to 20 days have been investigated in samples made with low and high extraction rate wheat flours following a sponge-dough process. White and whole-wheat enzyme-supplemented bread samples evidenced a similar sensory firming profile but a different quantitative instrumental staling pattern during storage. Two groups of samples with different staling behaviour can be defined according to the absence (faster staling kinetics) or the presence (slower kinetics) of NMYL in the bread formula, the separation being particularly clear for hardness, cohesiveness and resilience in white bread samples. TGM when added to NMYL-supplemented doughs induced synergistic beneficial effects on fresh bread quality and staling kinetics retardation. The binary combination led to breads with softer and less chewy fresh crumbs, increased initial crumb cohesiveness and resilience, and slower crumb staling kinetics and sensory deterioration during storage, particularly for samples made with white flour.     

The application of dextran compared to other hydrocolloids as a novel food ingredient to compensate for low protein in biscuit and wholemeal wheat flour

Wheat is primarily used for bread-making. However, fungal diseases, grain moisture at harvest and low-protein contents strongly influence the quality of the wheat flour, thus creating challenges for traders, millers and commercial bakers who struggle to produce consistently high-quality products. This paper address the replacement of low-protein/wholemeal flour functionality for bread-making purposes. Three hydrocolloids, xanthan gum, dextran and hydroxypropyl methylcellulose, were incorporated into bread recipes based on high-protein flours, low-protein flours and coarse wholemeal flour. Hydrocolloid levels of 0–5 % (flour basis) were used in bread recipes to test the water absorption. The quality parameters of dough (farinograph, extensograph, rheofermentometre) and bread (specific volume, crumb structure and staling profile) were determined. Results showed that xanthan had negative impact on the dough and bread quality characteristics. HPMC and dextran generally improved dough and bread quality and showed dosage dependence. Volume of low-protein flour breads were significantly improved by incorporation of 0.5 % of the latter two hydrocolloids. However, dextran outperformed HPMC regarding initial bread hardness and staling shelf life regardless the flour applied in the formulation.     

Different approaches for increasing the shelf life of partially baked bread: Low temperatures and hydrocolloid addition

Partially baked bread is a product with short shelf life that requires sub-zero temperatures for extending it. The storage of par-baked bread at low temperatures and the addition of bread improvers with antistaling effects, such as hydroxypropylmethylcellulose (HPMC), are very attractive alternatives for extending the shelf life of these products. In this study, staling during storage of partially baked bread (in the presence and absence of HPMC) at low temperatures (2 °C) is studied in terms of hardness increase and amylopectin retrogradation. Simultaneously, the staling of the derived full baked breads when stored at 25 °C is assessed. During the storage of par-baked bread at low temperatures, progressive crumb hardening and rapid crystallization of the amylopectin chains were produced. However, heat applied during full baking reversed those processes, and the extent of that improvement was dependent on the time of par-baked bread storage. Concerning the staling of the derived full baked bread, the time of par-baked bread storage did not significantly (P < 0.05) affect the staling process of the resulting full baked breads. The addition of HPMC decreased the crumb hardness in both par-baked and full baked breads, and also promoted a reduction of the amylopectin retrogradation. Overall results indicate that HPMC addition significantly retards the staling of par-baked bread during its storage at low temperatures and, moreover, the same effect is observed in the full baked bread.     

The influence of fenugreek gum and extrusion modified fenugreek gum on bread

Fenugreek gum (extruded and non-extruded) was substituted for wheat flour at 0%, 5% and 10% (w/w) and the rheological effects and breadmaking characteristics were determined. Bread containing fenugreek gum (FG) at 5% and 10% showed volumes and texture comparable with a control bread. Quality was high due to sheeting the dough twice as well as the use of the three-fold-turn puff pastry laminating method during processing of dough, instead of the standard rounding of dough. The three-fold-turn method is believed to have caused a higher incorporation of air into the dough as well as facilitated better distribution and incorporation of the fenugreek gum into bread dough. Extruding FG also improved its solubility in bread. Fenugreek gum (FG) resulted in an increase in dough farinograph water absorption compared with the control, but extruding the gum caused an even greater increase in water absorption when compared with the non-extruded gum. The addition of FG to bread dough caused an increase in G′ and G″. Starch pasting using RVA showed an increase in peak viscosity, final viscosity, breakdown and setback in a dose-related response when compared with a control.     

Effect of Sodium Stearoyl-2-Lactylate on the Rheological and Baking Properties of Wheat Bread Fortified with Defatted Soybean and Sesame Meal

Wheat flour was fortified with 8% DSBM (defatted soybean meal), 12% DSBM and 8% DSBM/4% DSM (defatted sesame meal) to increase the protein content and amino acid profile, and to upgrade the overall nutritional value of the bread. SSL (sodium stearoyl-2-lacty-late), at 0.5 and 1% levels, was incorporated into the three bread systems to overcome the deleterious effect of fortification on bread quality. Amylograph peak viscosity, dough elasticity, dough stability and bread volume improved when SSL was used. Addition of 1% SSL improved rheological properties and bread volume to a greater extent than 0.5% SSL. The control (100% wheat) bread had the best flavor, texture and color scores, followed by the 8% DSBM, 8% DSBM/4% DSM and 12% DSBM breads.     

糯小麦粉添加量对配粉流变学特性及馒头品质的影响

将糯小麦粉与普通小麦粉进行梯度配粉,研究不同糯小麦粉添加量对配粉糊化黏度、粉质特性和面团流变学特性的影响;采用质构和感官评价相结合的方法评价其对馒头品质的影响,确定合适的糯小麦配粉比。结果表明：随着糯小麦粉添加量的增加,配粉的糊化温度、峰值黏度、衰减度和回生值等快速黏度分析参数均呈现下降趋势,尤其是回生值降低显著。配粉的吸水量随糯小麦粉添加量的增加逐渐增大,面团的形成时间和稳定时间缩短,粉质指数降低;但当添加量增加至25.0%时,粉质特性各指标均产生显著性改变。不同配粉面团随着醒发时间的延长,面团拉伸曲线面积、拉伸阻力和拉伸比等指标均呈现一定的增大趋势,但延伸度却随醒发时间延长而降低。糯小麦粉配粉比例对馒头的质构和感官特性具有较大影响,适宜的糯小麦粉配粉比为20.0%～30.0%,25.0%配粉比使馒头具有较好的质构,感官总评分达到最大值。     

Effect of HPMC addition on the microstructure, quality and aging of wheat bread

The effect of hydroxypropylmethylcellulose (HPMC) addition on a basic bread formulation is described. The effect of HPMC as bread improver and antistaling agent was analysed in terms of microstructure. Bread quality was assessed by physical parameters (volume, width/height ratio, moisture content and hardness), crumb grain structure (number of air cells, cells area and the ratio between cells area and total area) and sensory evaluation (appearance, aroma, taste and texture). Bread staling was determined by following both the hardness increase and the starch retrogradation during storage. The microstructure was analyzed by cryo scanning electron microscopy (cryo-SEM). The results confirm the ability of the HPMC for improving fresh bread quality and for delaying staling. The presence of HPMC decreased the hardening rate of the bread crumb and also retarded the amylopectin retrogradation. The microstructure analysis revealed the possible interaction between the HPMC and the bread constituents, which could partially explain the antistaling effect of this hydrocolloid.     

Effect of baking conditions and storage with crust on the moisture profile,local textural properties and staling kinetics of pan bread

To investigate the impact of baking conditions on staling kinetics and mechanical properties, pan breads were baked at 180 °C/34 min and 220 °C/28.6 min using a ventilated oven and metallic moulds. After baking, bread slices were stored with and without crust at 15 °C in hermetic boxes for 9 days. This investigation provides a textural and physical analysis by examining the Young's modulus, crumb density and crust/crumb ratio during storage. In order to understand the relationship between firmness and moisture content, a moisture profile and a Young's modulus profile were determined during the storage of bread. To fit the staling, a first order model was used. It was found that the kinetics were faster for samples baked with a fast heating rate than for those baked with a slow heating rate. Moreover, the staling rate of bread stored with crust was faster than for bread without crust and the outer crust area staled more rapidly than the centre of the bread slice. These results suggest that the firming of the crumb is related to moisture distribution between the crumb and crust and to the impact of local baking conditions on local firmness.     

Promoting dough viscoelastic structure in composite cereal matrices by high hydrostatic pressure

The impact of high hydrostatic pressure (HP) treatment on dough viscoelastic reinforcement of highly-replaced wheat cereal matrices has been investigated. The gelatinisation/pasting and gelling profiles of HP hydrated oat, millet, sorghum and wheat flours, and the small and large deformation rheological parameters of blended wheat/non-wheat doughs were determined. Oat, millet, sorghum and wheat hydrated flours, at dough yield (DY) 160 and 200, were treated for 10 min at 0.1, 200, 350 or 500 MPa. Regardless the nature of the cereal, HP changes flour viscometric features, particularly in softer doughs (DY 200), leading to increased values for viscosity parameters, concerning pasting and paste cooking. Incorporation of 350 MPa pressure-treated flours into bread dough formulation provided increased dynamic moduli values, particularly for wheat and oat/wheat blends, associated to a reinforced dough structure. Highly-replaced composite dough samples treated at 500 MPa proved to be extremely stiff, resistant to stretch, low cohesive and low extensible, and thus not suitable for breadmaking.     

Effect of different carbohydrases on fresh bread texture and bread staling

The effect of cellulase, xylanase and #-glucanase on the properties of wheat bread and its staling during storage was studied. The presence of the carbohydrases tested led to breads with high specific volume compared to the control. The texture profile analysis was greatly modified in that the firmness of bread crumb was reduced by all the carbohydrases. A kinetic study of the firmness along with the storage by the Avrami equation showed that the presence of carbohydrases produced softer crumbs and also reduced the rate of bread firming, although no great differences were found between enzymes. Since retrogradation of starch is one of most important factors related to bread staling, the modification of the amylopectin retrogradation was measured by scanning calorimetry. Those studies showed that all the carbohydrases decrease the starch retrogradation, and that the xylanases had the greatest effect. The simultaneous analysis of the firming and starch retrogradation results revealed that the anti-staling effect of xylanase might be due to the retardation in the starch retrogradation, while in the case of cellulase and #-glucanase some other mechanism should be implied in their anti-staling action.     

Investigation of physicochemical properties of breads baked in microwave and infrared-microwave combination ovens during storage

Staling of breads baked in different ovens (microwave, infrared-microwave combination and conventional) was investigated with the help of mechanical (compression measurements), physicochemical (DSC, X-ray, FTIR) and rheological (RVA) methods. The effect of xanthan-guar gum blend addition on bread staling was also studied. Xanthan-guar gum blend at 0.5% concentration was used in bread formulation. The gums were mixed at equal concentrations to obtain the blend. After baking, the staling parameters of breads were monitored over 5 days storage. During storage, it was seen that hardness, retrogradation enthalpies, setback viscosity, crystallinity values, and FTIR outputs related to starch retrogradation of bread samples increased, whereas FTIR outputs related to moisture content of samples decreased significantly with time. The hardness, retrogradation enthalpy, setback viscosity, and crystallinity values of microwave-baked samples were found to be highest among other heating modes. Using IR-microwave combination heating made it possible to produce breads with similar staling degrees as conventionally baked ones in terms of retrogradation enthalpy and FTIR outputs related to starch retrogradation. Addition of xanthan-guar gum blend decreased hardness, retrogradation enthalpy and total mass crystallinity values of bread samples showing that staling was delayed.     

Staling white pan bread: fundamental causes

Staling, as it is applied to bakery foods, is a generic term covering a number of changes that occur in the products during normal storage. Consumers judge staleness by direct perception, which provides a subjective estimate that represents an unconscious integration of many factors. This review will discuss the main components of staling: (1) physicochemical changes of bread and related products (firming and texture deterioration of crumb and loss of crispness of crust) and (2) flavor changes. Section I will cover current theories of changes of firming and textural changes. The starch component of flour is generally considered to be responsible for these staling reactions. Consequently, the physicochemical involvement of amylose, amylopectin in these reactions will be fully discussed and current evidence supporting these theories (rheological, chemical, X-rays) will be given. Interactions of starch and surface active agents and other complexing compounds will be presented in Section II. In Section III, contribution of minor flour components and bakery food ingredients will be evaluated. Section IV will focus on organoleptic deterioration of products, presenting flavor changes that were observed during staling bread. Section V will discuss structural changes of breads caused by enzymolysis during bread production and storage as related to staling. Following the theoretical section (Sections I to V), Section VI will focus on practical control of staling. This discussion will cover the following factors: formulation, surfactants, enzymes, storage, freezing, and packaging.     

Effect of zein protein and hydroxypropyl methylcellulose on the texture of model gluten‐free bread

The influence of zein protein and hydroxypropyl methylcellulose (HPMC) on the texture and volume of gluten‐free bread was investigated. The addition of HPMC to starch affected the dough viscoelasticity and it improved the bread volume during baking since it acts as an emulsifier. The addition of zein protein to gluten‐free bread increased the crumb firmness and reduced the crust hardness within the range of concentrations investigated. No zein protein network could be observed in the bread crumb. The zein protein, cold mixed at low concentration, did not enhance the dough elasticity. Due to the lack of a protein network noncovalent interactions may stabilize the bubble structure stabilization within the crumb, rather than covalent links of the protein chain. With an optimized amount of zein protein and HPMC hydrocolloid, the gluten‐free bread showed similar texture and staling behavior to that of model wheat bread. The optimized recipe, compiled into a spreadsheet, is available in the supporting information. The microstructural observations suggest that zein could be replaced with another protein for this recipe resulting in a similar bread texture.     

硬脂酰乳酸钠对冷冻面团及其烘烤面包品质的影响

将硬脂酰乳酸钠(Sodium stearyl lactate,SSL)加入自制冷冻面团中,通过测定冷冻面团的流变学特性,以及冷冻面团烘烤面包的比容、质构特性和感官评分,研究SSL对冷冻面团及其烘烤面包品质的影响。结果显示,随着SSL添加量增加,冷冻面团弹性模量先升高后降低,损耗角正切tanδ先降低后升高,其中最优SSL添加量为0.20%;冷冻面团烘烤面包的硬度、咀嚼性先降低后升高,弹性、回复性、比容和感官评分先升高后降低,当SSL添加量为0.20%时,各指标达到各自的峰值,说明SSL改善了冷冻面团及其烘烤面包的品质。30 d冷冻储藏期内,加入SSL后,冷冻面团及其烘烤面包劣变幅度减小,说明SSL改善了其储藏特性。上述结果表明:加入SSL后,冷冻面团及其烘烤面包的品质及储藏特性得到改善,且最佳添加量为0.20%。