Serum level of galectin-3 and its clinical significance in patients with chronic Keshan disease

Objective: Keshan disease (KD) is a fatal endemic dilated cardiomyopathy with unclear etiology and pathogenesis, and a high mortality in China. Pathologic studies confirmed that different degree of myocardial fibrosis existed in various types of KD. Myocardial fibrosis is an important contributor to the pathophysiology of left ventricular remodeling. Recently, galectin-3 (Gal-3) as a marker of cardiac fibrosis and heart failure was approved by the US FDA. The aim of this study was to evaluate the changes of serum level of Gal-3 in chronic KD (CKD) and their clinical implications. Methods: The levels of serum Gal-3 were measured by using enzyme-linked immunosorbent assay (ELISA) in 37 CKD patients and 32 healthy controls. Echocardiography was used to determine parameter of left ventricular ejection fraction (LVEF) and left ventricular end-diastolic dimension (LVEDD). Results: The serum concentration of Gal-3 ([95.81±18.99] ng/ml versus [48.16±11.09] ng/ml, t=6.906, P<0.001) and LVEDD ([60.46±7.63] mm versus (42.69±10.66) mm, t=3.61, P<0.01) were significantly higher, while LVEF ([42.69±10.66]% versus [62.16±6.38]%, t=4.679, P<0.01) were significantly lower in CKD patients compared with healthy controls. A negative correlation was found between elevated Gal-3 and lower LVEF (r=-0.882, P<0.001) and a positive correlation was found between elevated Gal-3 levels and enlarged LVEDD (r=0.834, P<0.001) or higher NYHA class (r=0.854, P<0.01) in CKD patients. Conclusion: Serum concentration of Gal-3 is strongly correlated with poorer left ventricular systolic function, enlarged LVEDD and higher New York Heart Association (NYHA) class, which may provide indirectly diagnostic information on myocardial fibrosis and heart function in CKD patients.     

Protective Effect of Carvedilol on Abnormality of L-type Calcium Current Induced by Oxygen Free Radical in Cardiomyocytes

Carvedilol (Car)isanon selectivebeta blockerwithalpha receptorblockadeandantioxidantproper ties[1] .RecentCOMETtrialshowsthatCarextendssurvivalcomparedwithmetoprolol[2 ] .Theroleofox idativestresshasbeenexploredassuchamechanismofchronicheartfailure (CHF) …     

Therapeutic effect of 5-FC on YCD modified murine P388 leukemia in vivo

Cytosine deaminases (CDs) in bacteria andfungi are found to deaminate prodrug 5 - FC intocytotoxic agent 5 - FU .Yeast CD (YCD) is clonedfrom Saccharomyces cerevisiae.It has been shownpreviously that YCD was more efficient inconversing5 - FC than bacterial CD(b CD) [1-3 ] .As anovel suicide gene therapy system,YCD/ 5 - Fcsystem may be promising in cancer therapy andprevention of graft versus host disease.In thepresent study,we established a P388/ DBA murineleukemia model by infusin…     

The Inhibition of Apoptosis of Hepatoma Cells Induced by HBx Is Mediated by Up-regulation of Survivin Expression

Abnormalityofproliferationandapoptosisinma lignanttumorsisubiquitousandinvolvedmanygenes.Studiesshowedthathepatocellularcarcinoma(HCC) ,oneofthemostcommonmalignancesinChi na ,hasmanyabnormalexpressionofgenesrelatedtoproliferationandapoptosis .Recentstudiesindicatedthatsurvivin ,whichbelongstothefamilyofapopto sisinhibitionprotein ,hadahighexpressionlevelinHCCandwasrelatedtothemechanismsofabnormalapoptosisofHCCcells[1] .PatientswithHCCinChi nashowedahighinfectiousrateofover 80 %ofhep atit…     

Bipolar Esophageal Electrocardiography by Using Standard Limb Leads

Asearlyasin1906,CremerLl]firstre-portedanesophagealelectrocardiogram(ECG)recording.Sincethen,manystud-ies[2'3]0nthissubjecthavebeenundertakenandesophagcealECGshavebeensh0wntobesuperiortosurfaceECGsinidentificationofatrialactivityduringarrhythmia.InesophagealECGs,bipolarECGissuperiortounipolaroneandthefiltered0neissupe-riortothen0n-filteredone.AsthefilteredandbipolarECGrecordingsrequirespecialequipment,itwasimpracticalt0beusedatthebedside.Forbedsidediagnosisofar-rhythmia,HammilandPritc…     

冠脉支架置入术与冠脉搭桥术治疗严重冠心病的对比研究——SYNTAX研究

1 文献来源 Serruys PW, Morice MC, Kappetein AP, et al. Percutaneous coronary intervention versus coronary-artery bypass grafting for severe coronary artery disease [J]. N Engl J Med, 2009,360(10):961- 972.     

Effect of HDL and apo AI on PGE_2 Production by Monocyte-Derived Macrophages

Today,coronary artery disease( CAD) is oneof leading causes of death not only in the Westernworld,but also in Asian area including China[1,2 ] .Many studies showed that both high densitylipoprotein ( HDL) and its major protein compo-nent apolipoprotein AI( apo AI) are factors thathelp prevent CAD.It is widely believed their pri-mary function is to remove excess cholesterol fromextrahepatic tissue to liver for catabolism,a pro-cess referred to as reverse cholesterol transport,RCT[3 ] . …     

黎明前的黑暗，曙光将至——ARCS-M研究带来的启示

<正>1文献来源Kindler HL, Novello S, Bearz A, et al.Anetumab ravtansine versus vinorelbine in patients with relapsed,mesothelin-positive malignant pleural mesothelioma(ARCS-M):a randomised,open-label phase 2 trial[J]. Lancet Oncol,2022,23(4):540-552.     

Enhanced Radiosensitivity of Human Nasopharyngeal Carcinoma Cells by Coixenolide

Coixenolide(CXL) is a component extracted fromChinese herbal m edicine coix seed,and has the actions ofanticancer and imm unoregulation[1 ] .We previously reportedthat CXL could inhibit the growth of human nasopharyngealcarcinoma cells[2 ] 。 Clinically,however,irradiation is a primarytherapy in treatm ent of the tumor.The purpose of this paperis to study the effect of CXL on radiation to the carcinomacells.1　 Materials and methods1.1　 Cell line　 Cell line CNE- 2 Z (established in Gu…     

在高风险冠脉介入患者中IABP与Impella 2.5对血流动力学支持的对比研究

1文献来源 O＇Neill WW, Kleiman NS, Moses J, et al. A prospective, randomized clinical trial of hemodynamic support with Impella 2.5 versus intra-aortic balloon pump in patients undergoing high-risk percutaneous coronary intervention ： Circulation, 2012,126 The PROTECT Ⅱ study [J] （14） ： 1717-1727.     

Inhibition of Expression of Hypoxia-inducible Factor-1α mRNA by Nitric Oxide in Hypoxic Pulmonary Hypertension Rats

Hypoxiaisadirectfactorcausinghypoxicpul monaryhypertension (HPH) .hypoxiainduciblefac tor 1α (HIF 1α)isfoundtobethemostcrucialfactorsofarwhichmediatesthecellularresponsetohypoxi a[1] .OurpreviousstudyrevealedthatoverexpressionofHIF 1andendothelin 1(ET 1…     

Experimental Study of Assessment on Ventricular Activation Origin and Contraction Sequence by Doppler Tissue Imaging

Doppler tissue imaging(DTI) is a newly de-veloping technique that is based on color Dopplerimaging principles for evaluation of the ventricularwall motion by analyzing Doppler signals from car-diac tissues[1] .There are three DTI displayingmodes:acceleration mode,M- mode and spectralmode[2 ] .DTIcan be used to evaluate the position,size and severe degree of ventricular wall dysfunc-tion[2 ] .Recent studies demonstrated that the my-ocardial contraction sequence,activation origin andelectric…     

异基因造血干细胞移植后肠道移植物抗宿主病临床分析

移植物抗宿主病(graft versus host disease,GVHD)是异基因造血干细胞移植(allo-HSCT)后的主要并发症和死亡原因.肠道GVHD是急性GVHD的主要表现[1],其临床症状重,对全身状况影响大.我院行allo-HSCT患者中发生肠道GVHD 4例,现报告如下.     

The study on quantitative expression of CD44v6mRNA by real-time RT-PCR with the micro-metastases of gastric cancer

JNANJINGMEDUNIV2005;19(6):30330INTRODUCTION CD44isanintegralmembraneglycoprofeinwithanapparentmolecularmassthatrangesfrom85kDto250kd.Itwasoriginallydescribedasalymphocyto homingreceptoroncirculatinglymphocytes[4],cellad hesion,activationofleukocytes,andmigrationofcells.Atleast20variants(v)ofCD44havebeenreported[57]duetothealternativesplicingof10exons(v1v10)thatencodethemembranesproximalportionoftheextracellu lardomain.Guntherwasthefirsttoshowthattheex pressionofvariantsofCD44endow…     

Increased Neuronal Hypoxic Tolerance Induced by Repetitive Chemical Hypoxia

Repetitive administration of 3 - nitropropionate(3 - NP) ,a selective inhibitor of succinic dehydroge-nase,has been shown to cause chronic hypoxia fol-lowed by widespread neurodegeneration in centralnervous system,which further develops a pharmaco-logical animal model of Huntington' s disease[1] .Many scholars reported that single or repeated ad-ministration of 3 - NP causes widespread cerebral bio-chemical and morphological impairment[2 ] .On theother hand,it is well known that transient is…     

Whole Cag A Gene Amplification of Helicobacter pylori and Its Fingerprinting by Restriction Fragment Length Polymorphism

In recentyears,virulence factors of H elicobac-ter pylori ( Hp) have been focused on.In 1 988,Leunk first reported that Hp can produce a toxinleading to a vacuole- like pathology of eukareoticcells,called vacuole- associated cytotoxin A( Vac A) ,and found the toxin was produced in 5 5% of Hp[1] .In 1 990 ,Cover reported that anotherprotein encoded by a gene,cytotoxin- associatedgene A( cag A) ,is closely related to Vac A by us-ing immuno- blot[2 ] .Cag A has strong antigenicity.Ig G and …     

Pig model of chronic myocardial ischemia and its investigation by ultrasonic integrated backscatter and Doppler tissue imaging

Thedevelopmentandprogressofischemicheartdis ease(IHD)isacomplexandchronicprocess .Inourstudy ,weuseAmeroidconstrictortoproduceachronicischemiamyocardiummodelanduseIBSandDTItodetectitsoastoprovidemoreinformationforclinicalinvestigationandtreatmentofcoronaryheartdisease(CHD) .MATERIALSANDMETHODS　　Machine　HPSonos 5 5 0 0echocardiographicsystemwithaS4tranducer (frequency 2～ 4MHz) .　　Animalsandsurgicalprocedure　Fivedomesticpigs [weight,(2 0± 5 )kg]werestudied .Aftergeneralan…     

瑞波西利用于绝经后HR+/HER2-,luminal B型乳腺癌新辅助治疗：CORALLEEN研究

<正>1文献来源Prat A,Saura C,Pascual T,et al. Ribociclib plus letrozole versus chemotherapy for postmenopausa lwomen with hormone receptor-positive, HER2-negative,luminal B breast cancer(CORALLEEN):an open-label,multicentre,randomised,phase 2 trial[J]. Lancet Oncol,2020,21(1):33-43.     

β-adrenergic response modulated by κ-opioid receptor stimulation is attenuated in the cardiomyocytes of rats following chronic hypoxia

Objective: To study cross-talk between p-opioid receptor and β-adrenoceptor through determination of the intracellular calcium ( [ Ca2+ ]i ) and cAMP responses in ventricular myocytes of rats subjected to chronic hypoxia for 4 weeks. Methods: Electrically-induced [ Ca2+ ]i transient was measured in single right ventricular myocytes isolated from hearts of chronically hypoxic rats and the age-matched normoxic rats, by using a spectrofluorometric method. Results: β-adrenoceptor stimulation with isoproterenol increased the electrically-induced [ Ca + ]i transient and cAMP in myocytes of normoxic rats. U50,488H, a selective β-opioid receptor agonist, at dose (1 βmol/L) which itself had no effect on the [Ca2 + ]i transient and cAMP, significantly inhibited the effect of isoproterenol. This inhibition was completely abolished in the presence of nor-BNI, a selective K-opioid receptor antagonist. In the ventricular myocytes of chronically hypoxic rats, the inhibition of U50,488H on the increased [ Ca ]i tra     

Cerebral Ischemic Tolerance Induced by 3-nitropropionic Acid Is Associated with Increased Expression of Erythropoietin in Rats

Preconditioning of brain tissues with sub-lethal stresses or stimuli can result in resistance to subsequent lethal ischemic events in a response called ischemic tolerance[1] [2—4] . Recently, it was reported that such a neuronal tolerance could be induced by irreversible inactivation of succinate dehydrogenase (SDH), which is a mitochondrial complex Ⅱ (Krebs cycle) enzyme. SDH is the main respiratory electron transfer pathway into the plastoquinone (PQ) pool and could limit the damage inf…