胸腺肽对反复呼吸道感染病儿红细胞C3b受体活性的影响

目的观察胸腺肽对反复呼吸道感染（ＲＲＴＩ）病儿红细胞免疫黏附功能的影响。②方法采用酵母菌花环法检测胸腺肽治疗前后ＲＲＴＩ病儿红细胞免疫Ｃ３ｂ受体花环率（Ｅ－Ｃ３ｂＲＲ）和红细胞免疫复合物花环率（Ｅ－ＩＣＲ）的变化。③结果ＲＲＴＩ病儿感染急性期和间歇期Ｅ－Ｃ３ｂＲＲ皆明显低于对照组（Ｆ＝１５．５４,ｑ＝７．８７,４．３７,Ｐ＜０．０１）,而Ｅ－ＩＣＲ组间比较差异无显著性（Ｆ＝２．１１,Ｐ＞０．０５）。胸腺肽治疗后ＲＲＴＩ病儿Ｅ－Ｃ３ｂＲＲ显著增高,与治疗前比较差异有极显著性（ｔ＝６．５８,Ｐ＜０．０５）,而Ｅ－ＩＣＲ治疗前后差异无显著性。④结论ＲＲＴＩ病儿存在原发性红细胞免疫功能低下。胸腺肽对ＲＲＴＩ病儿红细胞免疫黏附功能有明显增强作用。     

铅中毒儿童红细胞免疫黏附肿瘤细胞能力的研究

目的探讨铅中毒儿童自然条件下的红细胞免疫功能和免疫黏附肿瘤细胞的能力。方法红细胞C3b受体花环率（RBC-C3bRR）和红细胞CIC受体花环率（RBC-ICRR）采用酵母多糖花环试验。红细胞免疫黏附肿瘤细胞（RIT）采用C3b介导的免疫黏附实验。结果铅中毒儿童红细胞免疫功能是降低的,主要表现为RBC-C3bRR、RIT明显降低,RBC-ICRR明显升高。结论铅中毒儿童红细胞免疫功能是低下的,铅对红细胞膜受体活性的损伤可能是铅中毒儿童红细胞免疫功能降低的重要原因。     

硒对红细胞免疫粘附肿瘤细胞能力的影响

采用红细胞Ｃ３ｂ受体花环试验和肿瘤红细胞花环试验，检测了硒对正常人和肿瘤病人红细胞Ｃ３ｂ受体活性及红细胞免疫粘附肿瘤细胞能力的影响。结果显示：①硒对正常人和肿瘤病人红细胞Ｃ３ｂ受体具有明显的激活作用，亚硒酸钠浓度为４×１０－４ｇ／Ｌ时，激活作用最强；②硒能明显增强正常人及肿瘤病人红细胞免疫粘附肿瘤细胞的能力，亚硒酸钠浓度为４×１０－４ｇ／Ｌ时，其增强作用最明显。     

宫颈癌患者红细胞免疫功能检测的临床意义

目的探讨宫颈癌患者红细胞免疫和肿瘤红细胞的功能状态。方法采用受体黏附法测定89例宫颈癌患者的红细胞免疫功能，并对43例患者作了手术前后的动态观察。结果宫颈癌患者红细胞C强受体花环（RBCC3bR）、自然肿瘤红细胞花环（NTER）、直向肿瘤红细胞花环（DTER）、促肿瘤红细胞花环（ETER）、协同肿瘤红细胞花环（ATER）及红细胞免疫黏附促进因子（RFER）显著降低（P〈0．01），红细胞免疫复合物花环（RBCICR）和红细胞免疫黏附抑制因子（RFIR）显著升高（P〈0．01），且与宫颈癌临床分期具相关性。术后各项指标与术前比较，差异有显著性（P〈0．01）。结论宫颈癌患者红细胞免疫功能低下，手术治疗有明显改善。     

缺铁性贫血患者红细胞天然免疫黏附功能测定及其临床意义

目的探讨缺铁性贫血(IDA)患者红细胞天然免疫黏附功能和免疫分子CD35的变化及其临床意义。方法采用红细胞酵母菌花环试验检测红细胞C3b受体花环率(RC3bRR)及红细胞促中性粒细胞吞噬率(ERPN),采用红细胞免疫黏附肿瘤细胞花环试验检测红细胞黏附肿瘤细胞花环率(RATR),采用流式细胞仪检测红细胞免疫分子CD35(RBC-CD35)数量。结果30例IDA患者治疗前,红细胞天然免疫黏附功能RC3bRR、ERPN及RATR与正常对照组比较显著降低,P<0.05;RBC-CD35数量与正常对照组比较显著降低,P<0.05。30例IDA患者铁剂治疗2、3个月贫血纠正后,RC3bRR、ERPN、RATR及RBC-CD35数量基本恢复正常,与正常对照组比较差异无显著性,P>0.05。结论IDA患者存在可逆性的红细胞免疫功能损害,强调IDA患者早期诊断及早期治疗的重要性。     

膀胱肿瘤20例红细胞免疫功能的测定

测定20例膀胱移行上皮细胞癌患者及30例健康人的红细胞免疫功能。结果发现:膀胱肿瘤患者红细胞C3b受体花环(RBC.C3bR)明显降低(P<0.01),而红细胞免疫复合物花环(RBC.ICR)显著升高(P<0.01),血清中免疫活性因子对红细胞C3b受体花环促进率降低,而抑制率升高;红细胞免疫粘附肿瘤细胞的能力(NTER、DTER、ATER)显著下降(P<0.01),血清活性物质对红细胞粘附肿瘤细胞的促进作用(ETER)亦显著下降(P<0.01)。     

脐血红细胞免疫功能的研究

测定了脐血红细胞免疫Ｃ３ｂ受体花环率、免疫复合物花环率、血清红细胞Ｃ３ｂ花环促进率、血清红细胞Ｃ３ｂ花环抑制率和肿瘤红细胞免疫花环率。结果表明：脐血红细胞免疫复合物花环率明显高于成年人，血清红细胞Ｃ３ｂ花环促进率和肿瘤红细胞免疫粘附花环率明显低于成年人，脐血红细胞Ｃ３ｂ受体花环率和Ｃ３ｂ花环抑制率与成年人无明显差异。     

大蒜素对恶性肿瘤患者红细胞免疫功能的影响

目的：探讨大蒜素对恶性肿瘤患者红细胞免疫功能的影响。方法：用红细胞Ｃ３ｂ受体花环试验（ＲＣＲＲ）、红细胞免疫复合花环试验（ＲＩＣＲ）、红细胞免疫粘附肿瘤细胞花环试验（ＴＲＣＲ）检测３０例恶性肿瘤患者服用大蒜素前、后１月、２月、３月及３０例正常对照组的红细胞免疫功能。结果：恶性肿瘤患者红细胞Ｃ３ｂ受体花环率及红细胞免疫站附肿瘤细胞花环经显示低于对照组（Ｐ〈０．０５）。而红细胞免疫复合物花环率显著高于     

脐血红细胞免疫功能的研究

测定了脐血红细胞免疫Ｃ３ｂ受体花环率，免疫复合物花环率，血清红细胞Ｃ３ｂ花环促进率，血清红细胞Ｃ３ｂ花环抑制率和肿瘤红细胞免疫花环率。结果表明：脐血红细胞免疫复合物花环率明显高于成年人，血清红细胞Ｃ３ｂ花环促进率和肿瘤红细胞免疫粘附花环率明显低于成年人，脐血红细胞Ｃ３ｂ受体花环率和Ｃ３ＢＳ     

单纯性肾病综合征病儿红细胞免疫功能的变化

目的 探讨单纯性肾病综合征（ＳＮＳ）病儿红细胞免疫功能状态及其临床意义。方法 采用红细胞酵母菌花环法和单克隆抗体间接免疫荧光法，检测了２４例ＳＮＳ病儿活动期和缓解期及３０例健康儿童红细胞Ｃ３ｂ受体花环率和红细胞免疫复合物花歪率及Ｔ细胞亚群的变化。结果 ＳＮＳ病儿活动期和缓解期Ｅ－Ｃ３ｂＲＲ皆显著低于对照组，活动期明显低于缓解期，而Ｅ－１ＣＲ皆无异常；活动期Ｅ－Ｃ３ｂＲＲ降低与ＣＤ４细胞和ＣＤ４／Ｃ     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.