Comparative investigations of gluten proteins from different wheat species I. Qualitative and quantitative composition of gluten protein types

In contrast to the hexaploid common (bread) wheat, little information is available on the qualitative and quantitative compositions of gluten proteins from other cultivated wheat species. Therefore, representatives of hexaploid spelt, tetraploid durum wheat and emmer, and diploid einkorn were compared with three classes of common wheat (winter wheat, spring wheat, wheat rye hybrid). The flours were extracted to yield total endosperm proteins and the gluten protein fractions (gliadins and glutenin subunits). The extracts were characterised using sodium dodecyl sulfate polyacrylamide gel electrophoresis and reversed-phase HPLC; both methods revealed that gluten protein groups and types known from common wheat (ω-, α-, γ-gliadins, HMW and LMW subunits of glutenin) were present in all species. The HPLC platterns of gliadins and glutenin subunits from species with the same genome composition (common wheat/spelt or durum wheat/emmer) were related, and those of einkorn quite different. According to the quantities determined by reversed-phase HPLC, α-gliadins were predominant in most cases, followed by γ-gliadins and LMW subunits; ω-gliadins and HMW subunits were generally minor components. Common wheats were characterised by the highest proportions of total glutenins and HMW subunits, which are known to be important for breadmaking quality. Moreover, the lower ratio of gliadins to glutenins was typical. Emmer had the lowest proportions of total glutenins and of HMW and LMW subunits, together with einkorn the highest proportion of α-gliadins, and, by far, the highest ratio of gliadins to glutenins. The values for spelt and durum wheat were mostly in a medium range between common wheats, emmer, and einkorn, respectively. Amongst common wheats, spring wheat was characterised by more balanced quantities of α- and γ-gliadins, and wheat rye hybrid by the highest proportions of ω-gliadins. Received: 26 November 1999     

The influence of 1B/1R chromosome translocation on gluten protein composition and technological properties of bread wheat

The Austrian bread wheat Amadeus without and with 1BL/1RS translocation and three further translocation genotypes with known HMW subunit compositions were grown under the same environmental conditions. Their flours were characterised by the determination of crude protein content and, partly, by the determination of glutathione and cysteine. Furthermore, the qualitative and quantitative composition of gluten protein types was analysed by a combined extraction and reversed phase HPLC procedure. Dough development time, maximum resistance and extensibility of dough and gluten, and bread volume were determined by means of microscale methods. Protein, glutathione and cysteine contents of flours were only slightly influenced by translocation. The HPLC patterns of gliadins and glutenin subunits showed that translocation caused characteristic changes concerning ω‐gliadins, γ‐gliadins and LMW subunits of glutenin. The amount of ω 1,2‐gliadins was significantly increased and that of LMW subunits decreased. The effect of translocation on the rheological properties of dough and gluten was characterised by a strongly reduced dough development time, reduced maximum resistance and increased extensibility. Bread volume was decreased by about 10%. The amount of glutenin subunits was correlated with dough development time, resistance of dough and gluten, and bread volume to a higher extent (r = 0.79–0.91) than the amount of gliadins (r = 0.52–0.80). Correlation coefficients for LMW subunits were higher (r = 0.82–0.88) than those for HMW subunits (r = 0.35–0.61) when all five wheats were included. Instead, when only translocation lines were considered, HMW subunits (r = 0.89–0.98) were more important than LMW subunits (r = 0.64–0.86). Altogether, the results demonstrate that translocation causes important quantitative as well as qualitative changes in gluten protein composition which can be efficiently determined by reversed phase HPLC. © 2000 Society of Chemical Industry     

Hearth bread baking quality of durum wheat varying in protein composition and physical dough properties

Thirty durum wheat genotypes from ten countries of origin were grown in field plots for two consecutive years. Three of the genotypes were γ‐gliadin 42 types and the remainder were γ‐gliadin 45 types. Among the γ‐gliadin 45 types, six high‐molecular‐weight glutenin subunit (HMW‐GS) patterns were identified: 6 + 8, 7 + 8, 7 + 16, 14 + 15, 20 and 2*, 20. All the γ‐gliadin 42 genotypes contained low amounts of unextractable polymeric protein (UPP) and exhibited low gluten index values and weak gluten properties. The γ‐gliadin 45 genotypes exhibited a wide range of UPP, gluten index and dough strength. HMW‐GS 20 genotypes were generally weak, whereas HMW‐GS 6 + 8 and 7 + 8 genotypes were generally strong. When baked by a lean formulation, long‐fermentation straight‐dough hearth bread process, the durum wheat genotypes exhibited a wide range of baking quality. Loaf volume and bread attributes were strongly correlated with UPP and gluten index. Some of the genotypes exhibited bread attributes and loaf volume equal or slightly superior to those of a high‐quality bread wheat flour. However, even the strongest durum wheat genotypes exhibited inferior fermentation tolerance to the bread wheat flour, as seen by a requirement for lower baking absorption during dough handling and more fragile dough properties when entering the oven. Among the HMW‐GS groups, HMW‐GS 7 + 8 and 6 + 8 exhibited the best and HMW‐GS 20 the poorest baking quality. Farinograph, alveograph and small‐scale extensigraph properties demonstrated that a combination of dough elasticity and extensibility was needed for superior durum wheat baking performance. Copyright © 2007 Society of Chemical Industry     

Comparative proteome analysis of glutenin synthesis and accumulation in developing grains between superior and poor quality bread wheat cultivars

BACKGROUND: Wheat glutenins are the major determinants of wheat quality. In this study, grains at the development stage from three wheat cultivars (Jimai 20, Jin 411 and Zhoumai 16) with different bread‐making quality were harvested based on thermal times from 150 °C_d to 750 °C_d, and were used to investigate glutenin accumulation patterns and their relationships with wheat quality. RESULTS: High and low molecular weight glutenin subunits (HMW‐GSs and LMW‐GSs) were synthesised concurrently. No obvious correlations between HMW/LMW glutenin ratios and dough property were observed. Accumulation levels of HMW‐GSs and LMW‐GSs as well as 1Bx13 + 1By16 and 1Dx4 + 1Dy12 subunits were higher in superior gluten quality cultivar Jimain 20 than in poor quality cultivar Jing 411 and Zhoumai 16. According to the results of two‐dimensional gel electrophoresis, six types of accumulation patterns in LMW‐GSs were identified and classified. The possible relationships between individual LMW‐GSs and gluten quality were established. CONCLUSION: The high accumulation level of HMW‐GSs and LMW‐GSs as well as 1Bx13 + 1By16 and 1Dx4 + 1Dy12 subunits contributed to the superior gluten quality of Jimai 20. Two highly expressed and 16 specifically expressed LMW glutenin subunits in Jimain 20 had positive effects on dough quality, while 17 specifically expressed subunits in Zhoumai 16 and Jing 411 appeared to have negative effects on gluten quality. Copyright © 2011 Society of Chemical Industry     

Wheat gluten: high molecular weight glutenin subunits--structure, genetics, and relation to dough elasticity

ABSTRACT:  Gluten proteins, representing the major protein fraction of the starchy endosperm, are predominantly responsible for the unique position of wheat amongst cereals. These form a continuous proteinaceous matrix in the cells of the mature dry grain and form a continuous viscoelastic network during the mixing process of dough development. These viscoelastic properties underline the utilization of wheat to prepare bread and other wheat flour based foodstuffs. One group of gluten proteins is glutenin, which consists of high molecular weight (HMW) and low molecular weight (LMW) subunits. The HMW glutenin subunits (HMW-GS) are particularly important for determining dough elasticity. The common wheat possesses 3 to 5 HMW subunits encoded at the Glu-1 loci on the long arms of group 1 chromosomes (1A, 1B, and 1D). The presence of certain HMW subunits is positively correlated with good bread-making quality. Glutamine-rich repetitive sequences that comprise the central part of the HMW subunits are actually responsible for the elastic properties due to extensive arrays of interchain hydrogen bonds. Genetic engineering can be used to manipulate the amount and composition of the HMW subunits, leading to either increased dough strength or more drastic changes in gluten structure and properties.     

The influence of nitrogen fertilisation on quantities and proportions of different protein types in wheat flour

The flours of 13 wheat varieties grown at different levels of nitrogen fertilisation were characterised by the quantitative determination of flour protein groups and gluten protein types using a combined extraction/HPLC procedure. The results demonstrate that the quantities of albumins and globulins were scarcely influenced by different nitrogen fertilisation, whereas those of gluten proteins (gliadins, glutenins) were strongly influenced. The effect on gliadins was more pronounced than on glutenins, as well as the effect on major protein types (α-gliadins, γ-gliadins, LMW subunits of glutenin) in comparison with minor types (ω-gliadins, HMW subunits of glutenin). The proportions of hydrophilic proteins (ω-gliadins, HMW subunits of glutenin) were increased by high levels of nitrogen and those of hydrophobic proteins (γ-gliadin, LMW subunits of glutenin) were decreased. The degree of the effects on both quantities and proportions of flour protein groups and gluten protein types was strongly dependent on the variety. © 1998 SCI.     

High‐molecular‐weight glutenin subunit composition of Pakistani hard white spring wheats grown at three locations for 2 years and its relationship with end‐use quality characteristics

Eleven Pakistani hard white spring wheat cultivars, along with one durum wheat and two hard white American‐grown wheat cultivars, were evaluated for their high‐molecular‐weight (HMW) glutenin subunit composition via sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE). The relationships among different quality characteristics and between these characteristics and HMW glutenin subunits were computed. Three to six HMW glutenin subunits were observed in Pakistani bread wheat cultivars. The presence of HMW glutenin subunits was not affected by growth locations or crop years. However, variations in intensities were observed. Correlations were noticed between certain HMW glutenin subunits and some quality attributes, such as protein, farinograph dough development time, farinograph water absorption, loaf volume and mixograph peak height. The presence of HMW glutenin subunit 20 in the older wheat cultivars C591 and C273, known for excellent chapati quality, indicated a possible relationship between this band and chapati quality. This observation will need to be confirmed by testing a larger number of wheat samples known to have characteristics for both good and poor chapati quality. © 2000 Society of Chemical Industry     

Chemistry of gluten proteins

Gluten proteins play a key role in determining the unique baking quality of wheat by conferring water absorption capacity, cohesivity, viscosity and elasticity on dough. Gluten proteins can be divided into two main fractions according to their solubility in aqueous alcohols: the soluble gliadins and the insoluble glutenins. Both fractions consist of numerous, partially closely related protein components characterized by high glutamine and proline contents. Gliadins are mainly monomeric proteins with molecular weights (MWs) around 28,000-55,000 and can be classified according to their different primary structures into the alpha/beta-, gamma- and omega-type. Disulphide bonds are either absent or present as intrachain crosslinks. The glutenin fraction comprises aggregated proteins linked by interchain disulphide bonds; they have a varying size ranging from about 500,000 to more than 10 million. After reduction of disulphide bonds, the resulting glutenin subunits show a solubility in aqueous alcohols similar to gliadins. Based on primary structure, glutenin subunits have been divided into the high-molecular-weight (HMW) subunits (MW=67,000-88,000) and low-molecular-weight (LMW) subunits (MW=32,000-35,000). Each gluten protein type consists or two or three different structural domains; one of them contains unique repetitive sequences rich in glutamine and proline. Native glutenins are composed of a backbone formed by HMW subunit polymers and of LMW subunit polymers branched off from HMW subunits. Non-covalent bonds such as hydrogen bonds, ionic bonds and hydrophobic bonds are important for the aggregation of gliadins and glutenins and implicate structure and physical properties of dough.     

Comparative quality analysis of gluten strength and the relationship with high molecular weight glutenin subunits of 6 Tunisian durum wheat genotypes

Six Tunisian durum wheat genotypes (4 landraces and 2 improved) were evaluated for protein content, gluten strength, rheological characteristics, and HMW-GS patterns using a LabChip system. Variance analysis identified genotypic variation. The landraces Azizi, Mahmoudi, Chili, and Arbi exhibited the highest protein concentrations and gluten contents, and best dough tenacity and extensibility values. The Mahmoudi and Chili varieties had the highest protein contents (17.06 and 17.32% dry mass, respectively). Arbi and Chili had the highest gluten contents (60.88 and 60.59%, respectively). Azizi, Mahmoudi, and Chili were characterized by higher dough tenacity, lower dough extensibility, and a greater alveograph configuration ratio P/L. The high molecular weight glutenin subunits 6+8 (Azizi and Mahmoudi) and 7+15 (Chili), coded by the Glu-B1 locus, improved gluten strength and viscoelastic dough properties. Calculated HMW to LMW-GS ratios were within a narrow range of 0.17–0.29. Some genotypes have potential to be used as parents in breeding programs.     

Rheological properties of gluten derived from wheat cultivars with identical HMW glutenin subunit composition†

Ten spring wheat cultivars possessing identical HMW glutenin subunits (2*, 7+8, 5+10) were evaluated for gluten and protein content. Gluten content was related to flour protein content (r=0·98). Addition of freeze-dried gluten to the base flour (cv Alpha) to a constant protein level of 12% generally increased dough strength. However, the magnitude of variation in mixing patterns depended more on the type of the supplemental gluten. Fortification of the base flour with the freeze-dried gluten from the cv Glenlea produced mixographs with the longest mixing development time (MDT), and highest band width energy (BWE) and energy to peak (ETP), suggesting that the source of gluten had a strong effect on dough rheology. The viscoelastic properties of undiluted wet gluten varied between cultivars during mixing reflecting differences in gluten quality. Freshly prepared wet gluten of Glenlea showed extended mixing tolerance as compared to Norseman or Alpha gluten. The wet gluten from cv Glenlea was less extensible with high maximum resistance to extension and had a larger area under the extensigraph curve than gluten obtained from cv Norseman. Gluten prepared from the cultivars Glenlea, Bluesky and Wildcat were less soluble in aqueous propanol and produced more froth when the dough was washed with deionised water. The froth proteins, separated by SDS-PAGE, predominantly contained strongly stained bands in the region corresponding to molecular weight <50 kDa. The rapid tests such as froth formation and alcohol solubility used in this study to discriminate various glutens were highly correlated with the mixograph parameters. These methods can be of practical value in evaluating gluten quality. © 1998 Society of Chemical Industry.     

不同储藏条件对小麦流变学特性的影响

为了解小麦籽粒在不同储藏条件下的品质变化,探索实验室小量样品的最适储藏条件, 本研究以强筋、中筋和弱筋小麦的籽粒和面粉为研究材料,对其在不同梯度温度（?20、4、35 ℃）和不同储藏时间（20、40、60 d）条件下的品质指标进行分析。结果表明:不同储藏条件对不同类型小麦的湿面筋含量、吸水量和弱化度的影响较小,均在允差内。以籽粒方式储藏时,强筋小麦在不同温度条件下各品质参数基本上都在允差范围内,但随着储藏时间的延长（60 d）,形成时间和拉伸面积超差。中筋小麦在不同温度条件下,湿面筋含量和粉质参数基本上都在允差内,但是对拉伸参数（拉伸面积、拉伸阻力和最大拉伸阻力）影响较大;尤其是当储藏条件为?20和35 ℃的情况下,除延伸性外,拉伸面积、拉伸阻力和最大拉伸阻力均有不同程度的超差。弱筋小麦在不同温度条件下湿面筋含量和拉伸各参数均在允差内,但对粉质参数（稳定时间和弱化度）影响较大,尤其是稳定时间。值得注意的是,弱筋小麦随着储藏时间的延长,粉质各参数测定值越接近对照,在允差范围内。另外,不同类型的小麦在4 ℃条件下以面粉方式储藏时,强筋和中筋小麦的湿面筋含量、流变学粉质参数和拉伸参数均在允差值内。因此,本研究认为4 ℃,≤60 d比较适宜小麦籽粒和面粉的储藏。     

Relationship between glutenin subunit composition and gluten strength measurements in durum wheat

Durum breeders use a range of techniques in the development of new cultivars. An important selection criterion is the rheological properties of semolina dough and durum wheat breeders use this criterion in the development of new cultivars using a range of techniques. Because of the need to process large numbers of genotypes encountered in breeding programs, methods that are inexpensive, rapid, require small amounts of sample and that correlate with semolina quality are desirable. Using breeding material, this study investigated the relationship between the glutenin subunit composition and two traditional tests of gluten strength, gluten index (GI) and mixograph. Two sample sets of durum wheat breeding lines and cultivars, one grown in Canada (n = 229) and the other grown in Australia (n = 139) were analysed for GI, mixograph and both high molecular weight (HMW) and low molecular weight (LMW) glutenin subunits by SDS‐PAGE. Nine different HMW and 14 different LMW allelic combinations were found. In the Canadian set, the most frequent LMW alleles were aaa, bba, caa and cfa while in the Australian set, caa was predominant. For the HMW subunits, the most common allelic groups were Glu‐A1c/Glu‐B1d (null, 6 + 8) and Glu‐A1c/Glu‐B1b (null, 7 + 8) with fewer numbers of Glu‐A1c/Glu‐B1e (null, 20) in both sample sets. LMW subunits were more important contributors to gluten strength than HMW subunits with the rank for higher GI according to the LMW allele (Canadian set) being caa = aaa > bba and aaa > cfa while HMW subunits 6 + 8 = 7 + 8 > 20. Similarly, using the mixograph, strength ranking for the LMW alleles was aaa > cfa = bba and HMW subunit 20 gave poorer rheological properties. For some samples with a good LMW allelic group a low GI was observed and vice versa. Further characterisation of the protein composition in these samples showed the GI results could be explained by polymeric/monomeric (P/M), glutenin/gliadin (Glu/Gli) and HMW/LMW ratios or the proportion of unextractable polymeric protein. © Crown in the right of the State of New South Wales, Australia; and for the Department of Agriculture and Agri‐Food, Government of Canada, © Minister of Public Works and Government Services Canada 2005. Published for SCI by John Wiley & Sons, Ltd.     

Role of gluten and its components in influencing durum wheat dough properties and spaghetti cooking quality

Protein is an important component of grain which affects the technological properties of durum wheat. It is known that the amount and composition of protein can influence dough rheology and pasta quality but the influence of the major classes of protein is not well documented. The influence of the various gluten components on dough and pasta properties was investigated. The protein composition of durum semolina was altered by either adding gluten fractions to a base semolina or preparing reconstituted flours with varying protein composition. The effects on semolina dough rheology and spaghetti texture were measured. Published methods to isolate relatively pure quantities (gram amounts) of glutenin, gliadin, high molecular and low molecular weight glutenin subunits were evaluated and modified procedures were adopted. Reconstituted flours with additional glutenin increased dough strength while additional gliadin and LMW‐GS decreased strength. These changes did not impact on spaghetti texture. Results from using the addition of protein fractions to a base semolina showed that gluten and glutenin addition increased the dough strength of a weak base semolina while gliadin addition weakened the base dough further. Addition of HMW‐GS greatly increased dough strength of the base while addition of LMW‐GS greatly reduced dough strength. Again, these affects were not translated into firmer pasta. Copyright © 2007 Society of Chemical Industry     

高分子量谷蛋白亚基近等基因系面粉品质及其流变学特性的研究

分析了高分子量谷蛋白亚基近等基因系的高分子量谷蛋白亚基组成，并对该近等基因系进行了品质性状的分析，结果表明：Glu-D1位点的变化比Glu-B1位点的变化对面粉品质和流变学特性影响大；并且同时具有Glu-B1 14 15和Glu-D1 5 10的近等基因系2号无论是面粉品质还是流变学特性都优于其它两个近等基因系8号(null，7 9，5 10)和近等基因系13号(null，14 15，10)；而8号又优于13号，即Glu-B1 14 15的存在没有弥补Glu-D1 5亚基的缺失。     

Effects of amounts of HMW glutenin subunits determined by capillary electrophoresis on technological properties in wheat doubled haploids

BACKGROUND: Knowledge of the types and amounts of individual HMW glutenin subunits (HMW-GS) is important for predicting technological quality. In this study, 228 wheat doubled haploids were compared in respect of HMW-GS composition, seed protein content, flour yield, rheological properties of dough, and loaf properties. The quantitative and qualitative composition of particular HMW-GS was determined by CE. The relative amounts of x-type and y-type subunits encoded by individual Glu-1 loci were considered. RESULTS: The results show that the amounts of some pairs x + y of HMW-GS are correlated with technological properties. The importance of quantities of particular pairs of HMW-GS for technological values depends on allele compositions in all the Glu-1 loci. Positive correlation was found between the amount of subunits Ax1, Ax2*, Bx7 + By8 or Dx5 + Dy10 and loaf volume (r = 0.215–0.618, P < 0.05). The influence of an increasing amount of Ax1 was positively correlated with dough softening (r = 0.451, P < 0.01) and negatively with dough development time (r = − 0.209, P < 0.05) and stability (r = − 0.351, P < 0.01), whereas for subunit Ax2* these associations were opposite. CONCLUSION: The results confirm the occurrence of epistatic interactions between alleles of Glu-1 loci. The effects of amounts of HMW subunits on bread-making quality should be considered only within glutenin subunit compositions. Copyright © 2008 Society of Chemical Industry     

谷朊粉和小麦淀粉添加量对重组面团冻藏品质的影响

面团的冷冻保存品质无法满足鲜湿面条工业化生产的要求。为了研究面团主要组分（面筋蛋白和淀粉）对面团冷冻品质的影响,以高筋小麦面粉（50%）、谷朊粉和小麦淀粉（不同比例）为原料进行面团重组,-18℃冻藏20 d分析其水分分布、流变特性、糊化特性、凝胶强度、微观结构以及氢键强度,以100%原小麦面粉作为对照组。结果表明,随着谷朊粉:小麦淀粉比例从4:1减小至1:4,冷冻重组面团中的水分分布逐渐由结合水向自由水迁移,弹性模量从125900 Pa降低至73020 Pa;样品的各项糊化参数增大,凝胶硬度也由114.30 g增大到181.39 g。扫描电镜观察发现,谷朊粉:小麦淀粉比例越低越不利于面筋蛋白网络结构的均匀性。添加了谷朊粉和小麦淀粉后,重组面团中的氢键强度均大于对照组,且随着谷朊粉:小麦淀粉比例的减小不断增大。当谷朊粉:小麦淀粉为4:1时,冻藏20 d的重组面团的弹性模量值比对照组高49.95%,有效延缓了面团在冻藏过程中的品质劣变。将淀粉与面筋蛋白进行面团重组可以提高面团的黏弹性,进而有利于其冷冻保存品质。     

Molecular Analysis of the Polymeric Glutenins with Gliadin‐Like Characteristics That Were Produced by Acid Dispersion of Wheat Gluten

We had earlier shown that the dispersion of wheat gluten in acetic acid solution conferred gliadin‐like characteristics to the polymeric glutenins. To elucidate the molecular behavior of its polymeric glutenins, the characteristics of gluten powder prepared from dispersions with various types of acid were investigated in this study. Mixograph measurements showed that the acid‐treated gluten powders, regardless of the type of acid, had dough properties markedly weakened in both resistance and elasticity properties, as though gliadin was supplemented. The polymeric glutenins extracted with 70% ethanol increased greatly in all acid‐treated gluten powders. Size exclusion HPLC and SDS‐PAGE indicated that the behavior of polymeric glutenins due to acid treatment was attributed to their subunit composition rich in high molecular weight glutenin subunit (HMW‐GS) and not their molecular size. The gluten prepared with the addition of NaCl in acid dispersion had properties similar to those of the control gluten. The results suggest that ionic repulsion induced by acid dispersion made the polymeric glutenins rich in HMW‐GS disaggregate, and therefore, act like gliadins.     

不同类型专用小麦HMW-GS和GMP含量与面筋含量的关系

高分子质量谷蛋白质亚基(HMW-GS)是决定小麦加工品质的重要因子,以25个小麦品种为材料,采用SDS-PAGE电泳方法研究亚基组成、各位点(Glu-A1、Glu-B1、Glu-D1)亚基积累量、谷蛋白大聚合体(GMP)含量及其与面筋含量的关系.研究结果表明:低蛋白小麦品种HMW-GS组成主要以"N、7+8/7+9、2+12"亚基组合为主,高蛋白小麦品种HMW-GS组成则主要以"1、7+8/7+9、5+10"亚基组合为主,中等蛋白小麦品种兼有以上两种类型亚基组合;亚基组成相同的小麦,籽粒GMP含量和蛋白质含量仍有较大差异.相关分析表明,HMW-GS亚基总积累量与籽粒蛋白质含量和GMP含量呈极显著正相关,与干、湿面筋含量成正相关;GMP含量与干、湿面筋含量、蛋白质含量均呈极显著正相关,说明小麦品质类型虽与HMW-GS组成有关,但亚基积累强度不同可能是导致籽粒品质类型差异的重要因素之一.     

The polypeptide composition,structural properties and antioxidant capacity of gluten proteins of diverse bread and durum wheat varieties,and their relationship to the rheological performance of dough

The aim of this study was to compare five bread and five durum wheat genotypes for gliadins and glutenins profiles, the concentration of free sulphhydryl groups and disulphide bonds, antioxidant capacity of gluten proteins and their bread‐making performance. On average, bread wheat had significantly higher concentration of total sulphur‐rich (S‐rich) and sulphur‐poor (S‐poor) subunits of gliadins, as well as total low molecular weight (LMW) and high molecular weight (HMW) subunits of glutenins than durum wheat. However, durum wheat had higher concentration of S‐rich γ‐gliadins and S‐poor D‐LMW‐glutenins, but did not possess S‐poor ω‐gliadins. The concentration of disulphide bonds and total cysteine was higher in the durum gluten than that in the bread gluten, as well as antioxidant capacity (on average 90.6 vs. 85.9 mmol Trolox Eq kg^?1, respectively). In contrast to the bread wheat, the concentration of HMW‐glutenins was negatively associated with extensibility, as well as resistance to extension in durum wheat flour dough.     

Optimization of Additive Combination for Improved Soy–Wheat Bread Quality

The protein content of ordinary white bread ranges from 8% to 9%. Specialty breads can be made with 13–16% protein by including soy protein. However, incorporating high levels of soy protein depresses loaf volume, gives poor crumb characteristics, and decreases acceptability. The objective of this study was to determine the influence of sodium stearoyl-2-lactylate (SSL), transglutaminase (TG), and xylanase (HE) on high-protein dough properties and bread quality and to improve dough handling and bread quality of soy–wheat bread by using an optimized additive combination. The influence of SSL, TG, and HE on soy–wheat dough and bread properties was modeled by response surface methodology. The negative effect of soy products on gluten network was confirmed. With regards to the additives tested and their combination, TG showed a major improving effect on dough rheological properties and crumb uniformity, whereas SSL and HE enhanced both dough and bread quality. The best formulation tested produced an increment of approximately 65% soy–wheat bread volume and a decrease of 79% and 71% crumb hardness and chewiness, respectively, compared with the standard formulation.