Expression of Fc- and C3-receptors on membranes of blast cells in myeloblastic leukemia and blast crisis of myelocytic leukemia

The expression of Fc-receptors to immunoglobulins IgG, IgA, IgM, that of FcH-receptor and receptors to C3-components of the complement in peripheral blood blast cells were studied in 11 patients with acute myeloblast leukosis and 10 patients with chronic granulocytic leukemia at the stage of blast transformation. The data obtained show the lower level of differentiation and functional activity of blast cells in patients with acute myeloblast leukemia while in chronic granulocytic leukemia a degree of expression of surface receptor on myeloblasts is close to the data obtained in the study of membrane receptors on promyelocytes and myelocytes of healthy persons.     

层流病房表面擦拭消毒的效果观察

利用压电陶瓷技术,设计并制备球面阵列压力传感器,并用于爆炸冲击波压应力的测试.结果表明,阵列传感器可测试爆炸冲击波的的压应力及其分布、体密度分布、面密度分布等参数,测试结果证明爆炸过程产生+的冲击波存在空间分布不均匀性.球面阵列压电陶瓷传感器的性能:测试单元数为136个,呈球面密排分布;不同单元的介电常数分布偏差小于±2.5%;压电常数分布偏差小于±2%;动态响应频率约为1 MHz;冲击破坏阈值为100 MPa量级.     

利用波长调制光谱的燃烧场温度原位测量

针对高炉煤气成分复杂、燃烧效率低、燃烧稳定性差的问题,提出了基于波长调制光谱（Wavelength Modulation Spectroscopy,WMS）的高炉煤气燃烧场温度测量方法。WMS具有抗噪声能力强、测量精度高和灵敏度好的特点,适用于高炉煤气燃烧场的温度测量。基于可调谐二极管激光吸收光谱技术的测量特点,以H2O为目标气体,选取波长位于1 391.67 nm和1 397.75 nm的吸收谱线,通过两个激光器时分复用的方式获取两条目标吸收谱线,对黑体炉和平面火焰燃烧炉进行温度测量,达到了实验验证的目的。实验结果表明,所提检测方案对高炉煤气所在500~2 000 K温度范围内都有较高的测量灵敏度,检测结果线性度优于99%。现场实验验证系统可满足高炉煤气的温度场测量等原位在线测量的应用,为后续的燃烧优化和节能减排奠定了基础。     

The correlation of the in vitro 14C-glycine absorption by murine and human leukemic cells with the antitumor effect of vincristine

Vincristine (VCR) studied for its in vitro effect on glycine accumulation by mouse leukemic cells and blood blast cells of patients with hemoblastosis. It is found that the value of glycine cell accumulation and a degree of inhibition of the initial level of amino acid accumulation in a cell by alkaloid correlate with the antitumour action of the preparation. The glycine accumulation by blood blast cells (less than 40 nmol/min/10(9) cells), absence of the VCR effect on this process are found to be unfavourable prognostic factors for successful chemotherapy of patients by VCR. The possible role of the cellular membrane in the mechanism of the VCR action is discussed.     

Colony-forming ability of bone marrow in children with acute leukemia

Colony- and cluster-forming ability of bone marrow cells in agar medium was studied in 6 healthy children and 13 children suffering from acute leukosis. During acute stages of the diseases colonies and clusters in agar medium consisted of granulocytic cells and their quantity depended on the safety of predecessors of bone marrow granulopoiesis. In contrast to acute lymphoblastic leucosis, acute myeloblastic leucosis is characterized by a sharp growth of cluster-forming and a less intensive growth of colony-forming ability of hemopoietic cells. The bone marrow remission is marked by the growth of diffusive and mixed colonies and clusters, consisting of granulomonocytic elements. The low level of colony- and cluster-formation in the medium at the last stage of therapy (induction of acute leukosis remission) correlated well with positive indices of treatment effectiveness. The results obtained during the cultivation of blood-forming cells in the agar medium may be also used for diagnosis and prognosis.     

ICO-35 monoclonal antibodies to the antigen of acute lymphoblastic leukemia

ICO-35 monoclonal antibodies (Mabs) were produced following BALB/c mouse immunization with peripheral blood cells from a patient with lymphoid type of chronic myeloid leukemia in blast crisis (SML BC) ICO-35. Mabs detect antigen on CD10-positive cells from patients with acute lymphoblastic leukemia, CML BC, CD10-positive cells of Reh line and are not bound to other cells. Comparative studies of reactivity of ICO-35 and K 503 Mabs to CD10 antigen revealed their similarity. However, in contrast to K503 ICO-35 Mabs do not react with granulocytes.     

In vitro 3H-vincristine accumulation as a possible marker of the sensitivity of animal and human leukemic cells to the preparation

The uptake of 3H-vincristine into the leukemic cells of animals and blast cells of peripheral blood of patients with leukemia has been studied. The capacity of cells to accumulate the drug is found to be of significant value for their sensitivity to vincristine. The in vitro low-alkaloid accumulation (less than 18 pmol/mg protein) by cells is an unfavourable prognostic sign for vincristine chemotherapy.     

不同约束下激光推进流场波系结构实验研究

利用透镜聚焦CO2激光击穿空气,在有效解决实验系统时序问题的基础上,借助高速阴影系统研究了3种不同约束构形下激光推进的流场波系结构,获得了波系演化的时序照片,并初步分析了等离子体点火特性和激光支持吸收波(之后退化为激波)的传播速度.结果表明:无约束下流场约呈椭圆形扩散衰减,平面靶和15°扩张靶约束下发生明显的激波反射和边界绕射现象,3种约束中心均出现缓慢变化的低密度高压气团;3种约束下激光支持的爆轰波退化为激波时速度急剧下降,之后激波速度变化平缓,约70～80μs降至声速.     

高压变频器在高炉矿槽除尘系统中的应用

本文通过对炼铁厂矿焦配料系统生产工艺和配套矿槽除尘系统的环保生产分析,结合高压变频调速技术的应用特点,以实际应用案例阐述高压变频器在矿槽除尘系统中的环保、节能、高效的应用效果和显著效益。     

Changes in the biochemical parameters of lymphocyte functional activity in simian adenovirus SA7(C8)-induced carcinogenesis

A functional defect of lymphocytes in hamsters with tumours during carcinogenesis induced by monkey adenovirus SA7(C8) was found and estimated by means of the blast transformation reaction. An increased activity of transport ATPases, the disturbance of cyclic nucleotide levels (cAMP, cGMP) and a decrease in both basal and Con A-activated calcium transportation were observed simultaneously with changes in the activation of immunocompetent cells under Con A influence.     

Preparation and characterization of perovskite-type Titania-bearing blast furnace slag photocatalyst

Perovskite-type titania-bearing blast furnace slag (TBBFS) photocatalysts were prepared by the high-energy ball milling method at different calcination temperatures. The particle size and ultraviolet light-absorption capacity of titania-bearing blast furnace slag powders increased with increase in the calcination temperature. Its photocatalytic activity was checked through the photocatalytic reduction of Cr(VI) as a model compound under ultraviolet–visible light irradiation. Titania-bearing blast furnace slag powders calcined at 700 °C showed a higher catalytic activity compared to other titania-bearing blast furnace slag catalysts, indicating that the increase in calcination temperature could improve their photocatalytic activities.     

Polyamine levels and biosynthesis in leukocytes during leukemic transformation

The content of polyamines, rate of their biosynthesis, and activity of ornithine decarboxylase (ODK), the key regulatory enzyme in the polyamine biosynthetic pathway, have been studied in the leukocytes of healthy persons and of patients with various forms of leukemia. The polyamine levels and the rate of their biosynthesis were considerably higher in lymphocytes than in granulocytes. In leukemias, as the cell population rejuvenated, both the polyamine content and the spermidine/spermine ratio were found to be markedly elevated approaching the maximal values in human blast cells of K-562 line. The leukemic transformation of myeloid cells was associated with the increase in the ornithine decarboxylase activity and in the rate of polyamine synthesis, whereas these items were significantly lowered in lymphoid leukemic cells. The changes observed in polyamine metabolism in leukemias are considered to be related to the proliferative and biosynthetic activity of the cells.     

Monoclonal antibodies ICO-02 to blast cell antigens in patients with chronic myeloleukemia in blast crisis

Mice were immunized with blood cells of a patient with chronic granulocytic leukemia, and their cells were subsequently used for the preparation of hybridoma ICO-02. This hybridoma is continuously producing monoclonal antibodies which reacted with cells in 4 out of 13 patients with blastic crisis of chronic granulocytic leukemia and in 6 out of 38 patients with acute lymphoblastic leukemia. Antibodies reacted with blast cells in 2 out of 3 patients with undifferentiated blastic crisis of chronic myelocytic leukemia and in 2 out of 5 patients with lymphoid variant of blastic crisis of chronic granulocytic leukemia. Cells of 6 patients with acute lymphoblastic leukemia which reacted with the monoclonal antibodies had immunological markers of T lymphocytes bone-marrow precursors. Monoclonal antibodies did not react with cells of blood and bone marrow from healthy people and from patients with chronic lymphocytic leukemia, acute myeloblastic leukemia, acute myelomonocytic leukemia, acute monoblastic leukemia and lymphosarcoma.     

Hematopoietic changes in mice with transplantable Lewis lung carcinoma

The cell composition of bone marrow, spleen, and peripheral blood as well as the content of early granulocytic and macrophagal precursor cells forming colonies in agar cultures in diffusion chambers in vivo (CFU-DC) were studied in C57B1/6 mice during the Lewis carcinoma growth after subcutaneous transplantation of tumour cells. It has been shown that in peripheral blood of such mice neutrophilic leucocytosis develops and the content of CFU-DC increases considerably. The spleen has become a source of surplus production of granulocytic and macrophagal cells from the early precursors (CFU-DC) to the mature cell forms with their delivery to the peripheral blood. No essential changes in the cell composition and CFU-DC content were found in the bone marrow of femur.     

Sensitivity of clonogenic cells of human ascitic ovarian cancer to antitumor agents

A soft agar colony assay using diffusion chamber technique in vivo for studying the sensitivity to chemotherapeutic agents of clonogenic tumor cells of primary ovarian carcinoma was used. The highest cytotoxic sensitivity on human clonogenic tumour cells was observed to cyclophosphamide and 5-fluorouracil. The sensitivity of human ovarian carcinoma to chemotherapeutic agents was dependent essentially on the presence of macrophage in cell culture.     

Microdomain structure of agar gels observed by mechanical-scanning probe microscopy

The inhomogeneous structure of agar gels was examined by means of mechanical-scanning probe microscopy. Several domains were observed in the elasticity images, while such domains could not be seen in the height images. The domain size decreased with increases in agar concentration. We found that the histograms of the logarithm of the local elastic modulus were described well by a single normal distribution. As the agar concentration increased, the peak values of the histograms increased, while the half-value width remained constant. These results imply that the gelation process of agar gels has a common mechanism, despite its complexity.     

Antibody-dependent cytotoxicity of lymphocytes in acute myeloblastic leukemia and chronic myelocytic leukemia

Antibody-dependent cytotoxicity (ADC) of peripheral blood blast cells in vitro was studied in 16 patients with acute myeloid leukemia (AML), 6 patients with acute monoblastic leukemia (AMonL), and 13 patients with acute myelomonoblastic leukemia (AMmL). It was found that monoblasts have a higher degree of ADC activities with respect to target cells than myeloblasts. Functional differences between monoblasts and myeloblasts may be used as criteria for differential diagnosis of acute leukemia variants.     

Tumor-induced immunosuppression in breast cancer patients

A comparative study of immunological reactivity of healthy volunteers and cancer patients with different stages of the disease was carried out. The study of 8 types of suppressor cells, chemoreceptor sensitivity and blast transformation of lymphocytes, natural killer cells, the level of immunoglobulins, regulatory subpopulation of lymphocytes, etc., has revealed several alterations in regulatory and effector links of immunity in the patients with late stages of the disease. The detailed immunological monitoring in the course of varying schemes of treatment is emphasized.     

Surface membrane receptors, differentiation antigens and cytochemical characteristics of blood cells in acute lymphoblastic leukemia in children

The study of surface membrane receptors by the methods of combined patch-formation and cytochemical markers of blast cells has revealed 57 cases of childhood ALL which were classified as non-T, non-B cell, pre-T, T- and B-cell forms. Using the panel of monoclonal antibodies (IKO-1, 02, 10, 11 and I10P-1-8) the surface differentiation antigens have been studied in certain patients. Significance of cytochemical studies for definition of main forms of acute leukemias and immunocytology for differentiation of some types of ALL was suggested.     

ICO-10 monoclonal antibodies to the Thy-1 antigen

Mouse monoclonal antibodies (MAB) ICO-10 to Thy-1 antigen were obtained. MAB ICO-10 reacted in indirect immunofluorescence test with 5.7 +/- 0.8% human thymocytes. Antibodies did not react with granulocytes, monocytes, T- and non-T cells from peripheral blood, and with marrow cells of healthy donors. MAB ICO-10 reacted with blast cells from 25 of 53 patients with T-cell acute lymphoblastic leukemia (ALL), from 2 of 5 patients with B-cell ALL. This antigen was absent on blood and marrow cells from some patients with ALL, 80 patients with chronic lymphoid leukemia, 54 patients with chronic granulocytic leukemia at the stage of blastic crisis, 128 patients with acute nonlymphoblastic leukemia. Antibodies are specifically bound to thymocytes and spleen cells of Thy 1.1 and Thy 1.2 mice. MAB ICO-10 detect Thy-1 antigen expressed on human hematopoietic cells. MAB ICO-10 may be applied for human leukemia and lymphoma immune diagnosis.