Isolation of mononuclear cells from the bone marrow of patients with hemoblastoses using one-step ficoll-verographin density gradient separation

The one-step ficoll-verographin density-gradient centrifugation was first used for isolating mononuclear cells from human bone marrow. Children suffering from various hemoblastoses were donors. The morphological control of the cells isolated from the interface layer and May-Grünwald stained showed a high efficiency of the procedure proposed. Due to the nosological form of malignancy mononuclears composed from 100% (in the relapse of acute lymphocytic leukemia) to 81% (in hemoblastosis with "intact" bone marrow: lymphogranulomatosis and lymphosarcoma without leukemization) among the isolated cells. Thus, the one-step ficoll-verographin density-gradient centrifugation may be used for extensive clinical research, permitting more time-consuming procedures to be avoided.     

露蜂房蛋白对急性髓细胞白血病患者骨髓单个核细胞超微结构的影响

目的：观察中药露蜂房蛋白(NVP)成份对急性髓细胞白血病(AML)患者骨髓单个核细胞(BMMNC)超微结构的影响。方法：采用体外细胞培养,加入不同浓度露蜂房蛋白成分作用于AML患者BMMNC,培养72h收集细胞,常规制备超薄切片,透射电子显微镜下观察AML患者BMMNC的超微结构。结果：露蜂房蛋白各处理组细胞核染色质浓缩、边集,呈新月形或环状或细胞核碎裂呈块状。线粒体出现空泡样变及髓样变。结论：中药露蜂房蛋白成份有明显诱导AML患者体外培养BMMNC凋亡的作用,并呈现典型的细胞凋亡超微结构改变。     

体外培养兔骨髓间充质干细胞的超微结构观察

本文对体外培养的新西兰大白兔骨髓间充质干细胞 (ＭＳＣｓ)的超微结构进行了电镜观察 ,为选择良好的细胞生长状态 ,应用于组织工程提供实验依据。分别对原代和传代后第二、三、四、五代细胞进行了透射电镜观察。结果原代及第二、三代细胞胞核呈圆或卵圆不规则形 ,双层核膜完整 ,核仁大而明显 ,部分细胞见有 2～ 3个核仁 ,常异染色质分布均匀。胞浆丰富 ,呈中等电子密度 ;细胞器较简单 ,但数量丰富 ,结构清晰 ,如粗面内质网、滑面内质网、线粒体等 (图 1)。第四、五代细胞透射电镜下见有部分细胞开始出现结构异常现象。有些细胞的细胞核内异…     

Ag-negative phenomenon of nucleolar organizers of bone marrow and blood cells in children with acute lymphoblastic leukemia]

The activity of nucleoli-organizing (NOR's) regions was studied in leukemic cells of 53 children with immunologically typical ALL. The Ag-negative type of ALL was found in 9 cases of IA-ALL and Comm(+)-ALL. Dominating cells were not argentaffin. Some cytological peculiarities of Ag-negative cases have been demonstrated: lower presence of E(+)-receptor on circulating lymphoid cells, the greater number of cells with smooth surface which was revealed by the scanning electron microscopic method. The cases when the product of Ag-philic reaction was impossible to be revealed are discussed.     

海藻多糖对小鼠骨髓放射性急性损伤的保护作用

目的 :观察中药海藻多糖对小鼠骨髓放射性急性损伤的保护作用。方法 :检测小鼠骨髓有核细胞总数、DNA含量及嗜多色性红细胞微核率。结果 :小鼠接受 2 Gyγ射线照射后 ,小鼠骨髓有核细胞总数、DNA含量明显降低 (P<0 .0 1) ,骨髓嗜多色性红细胞微核率明显升高 (P<0 .0 1) ;2 0、40 mg/kg剂量的海藻多糖能有效防止照射小鼠骨髓有核细胞总数、DNA含量降低 (P<0 .0 5 ) ;10、2 0、40 mg/kg剂量的海藻多糖能防止照射小鼠骨髓嗜多色性红细胞微核率升高 (P<0 .0 5 )。结论 :中药海藻多糖预防性给药对小鼠骨髓放射性急性损伤有一定的保护作用     

激光扫描共聚焦显微镜在骨髓基质细胞移植中的应用

目的：探讨激光扫描共聚焦显微镜（laser scanning confocal microscope,LSCM）技术在骨髓基质细胞（Bone Marrow Stromal Cells,BMSCs）移植中的应用。方法：使用活细胞示踪剂CM-DiI、细胞核探针DAPI和DNA合成前体物BrdU体外标记BMSCs,移植到动物模型体内,利用LSCM技术,观察BMSCs在受体组织内的分布和分化。结果：体外标记BMSCs后再移植到受体,使用LSCM技术可以观察到BMSCs定位于受体心肌组织和脑组织,并有部分BMSCs表达相应的组织特异性蛋白Cx43和GFAP。结论：利用LSCM技术观察BMSCs在受体组织内分布和分化是一种简单可行的方法。     

The effect of autoplasma of patients with breast cancer on the functional activity of natural killer cells and adhering mononuclear cells in peripheral blood in vitro

Autoplasma of 43 breast cancer patients and 30 donors has been studied for its effect on the activity of natural killer (NK) cells and adherent cells phagocytosis. The cytotoxic activity of NK cells and the phagocytic activity of adherent cells as well as the cytotoxic activity of plasma of breast cancer patients toward K-562 cells are found to increase during tumour progression. Autoplasma of cancer patients is shown to block the cytotoxic activity of NK and even more markedly--the phagocytic activity of adherent cells. As a result, breast cancer patients have nearly normal level of the cytotoxic activity of NK in the presence of autoplasma, whereas the phagocytic activity of adherent mononuclears decreases. Thus, autoplasma of breast cancer patients properly affects the cytotoxic activity of the NK cells and the phagocytic activity of adherent cells.     

Modifying effect of the blood serum from patients with soft-tissue sarcomas on interleukin-2 production and activity

Interleukin-2 (IL-2) production by lymphocytes of a peripheral blood (LPB) in patients with soft tissue sarcomas is considerably lower in comparison with that in donors. Autological serum had either suppressive or stimulative effect on LPB IL-2 production in the observed patients. The investigated serum affected LPB of donors in the same way. Serum of patients with soft tissue sarcomas had the same dual effect on the ability of the active drug IL-2 to proliferate human T-lymphoblasts. Moreover serum-inhibitors of production acted as IL-2 activity stimulators, while serum-stimulators of IL-2 production inhibited the mediator effects.     

The dependence of the clonogenic fibroblast proliferation of human bone marrow on the feeder

The proliferation of human bone marrow stromal fibroblasts depends on the growth factors. Xenogenic bone marrow cells, previously radiated in a dose of 3000 Gy, are shown to be a source of such factors. The human bone marrow cells contain both stimulators and inhibitors of the fibroblast proliferation. The inhibitory activity of the bone marrow cells increases with their concentration in explants. The optimal culture conditions are developed. The efficiency values of the fibroblast cloning in children and adults are presented and compared.     

基于压电效应的智能控制广场自发电系统

本文介绍了一种基于压电效应的智能控制广场自发电系统,核心技术是通过压电材料的压电效应将机械能转化为电能,并通过能量转换模块和能量储存模块将电能储存于锂电池中,给主控制器模块即Arduino供电,主控制器模块驱动灯光和音频输出模块.整个装置所需的电能都通过压电效应产生,起到节能环保的作用.     

The effect of asynchronism on the total capacity of Gaussianmultiple-access channels

The degradation due to complete asynchronism (at the codeword and symbol levels) in the total capacity, maximum rate-sum, of white Gaussian multiple-access channels is investigated. It is shown that asynchronism reduces the total capacity of a K-user channel by at most a factor of K. Moreover, this bound is achieved, in asymptotically high signal-to-noise ratios, by the TDMA signaling strategy. When the signaling strategies are optimally designed to maximize the asynchronous total capacity under bandwidth constraints, the authors find that in a two-user channel: (1) for a certain set of signal-to-noise ratios there is no degradation due to asynchronism, (2) for any bandwidth and signal-to-noise ratios the asynchronous total capacity is at least 88% of the synchronous total capacity, and (3) asynchronism has a vanishing small effect on total capacity for both low and high signal-to-noise ratios     

提高mdr1基因体外转染骨髓造血细胞效率的实验研究

目的：探讨mdrl基因体外转染兔骨髓造血细胞的条件及转染后在细胞中的功能性表达。方法：秋水仙碱（90ng／ml）筛选含人mdrlcDNA的产病毒包装细胞PA317-HaMDR1／A,制备浓缩病毒上清液并转染兔骨髓单个核细胞;分别用PCR法、免疫组织化学法和柔红霉素（daunorubicinDNR）排出试验检测mdr1基...     

The effect of adjacent channel interference on the capacity of FDMAcellular systems

In FDMA cellular radio systems, the adjacent channel interference (ACI) is suppressed by the IF filter and also by a distance factor. Channels immediately adjacent to each other in frequency are assigned to different cells and, as such, the distance plays a role in reducing their mutual interference. The authors study the feasibility of optimizing the frequency spacing between carriers such that the combined effect of distance and IF filter will be enough to control the interference while allowing the maximum number of channels in a given bandwidth. They also study the decision feedback equalizer (DFE) as a means to reduce any excess ACI that results from reducing the channel spacing. The paper starts with a model that estimates the cumulative probability density of the signal to adjacent channel interference ratio (SIR) as a function of the channel spacing. They then present the results of a computer simulation study to estimate the performance of DFE in mitigating the effect of ACI. The results indicate that, under the conditions of slow flat fading and selection diversity, the DFE is very effective in reducing the effect of excess ACI interference which allows for a considerable reduction in the frequency spacing     

The effect of collector capacity on the transient response of junction transistors

The effect of collector depletion layer capacity on the transient response of junction transistors to a current input is calculated for the case of a resistive load. Expressions are given for the small-signal rise-time of the common-base, emitter, and collector configurations and for the large-signal turn-on and decay times of the common-emitter and collector configurations. The analysis shows that the transient durations under most conditions of operation are approximately (1 + omega_{alpha}R_{L}C_{c}) times those which would be predicted for the short-circuit output approximation reported by Moll [1]. Experimental results are reported which exhibit an excellent agreement with the analysis over a wide range ofomega_{alpha}R_{L}C_{c}. An empirical examination has been made of the dependence of large-signal switching time on the range of operating point excursion. A satisfactory approximate representation of this dependence is provided by a first-order correction factor, which takes into account the functional dependence of ωαand Ccon collector voltage.     

Functional activity of the thymus gland and pituitary-adrenal system in patients with malignant skin melanoma

Essential changes in the thymus functional condition and hypophyseal-adrenal system were revealed in patients with skin melanoma. The secretory activity of the thymus loosens, and there is a reduction of thymic hormone titer in blood. The increased levels of ACTH and hydrocortisone in blood indicate an enhanced activity of the hypophyseal-adrenal system under this pathology.     

Effect of bleomycetin on the proliferative activity of bone marrow and lymph node cells in SJL/J mice

Dynamics of dose and time dependence of bleomycin action on bone marrow and lymph node cells of SJL/J mice has shown that 6 h after administration of 25 micrograms of bleomycin the proliferative activity is 1.5 times reduced. The antibiotic influences the interphase cells and delays a cellular cycle. A durable and persistent decrease of the proliferative activity (up to 10 days) of bone marrow and lymph node cells shows a prolonged action of the bleomycin single dose and a pronounced cytostatic effect of the antibiotic on cellular elements of SJL/J mice. Bleomycetin is established to produce action both on lymph node and bone marrow tumour cells of SJL/J mice.     

Activity of the natural killer cells in the peripheral blood of patients with brain tumors

Activity of natural killer cells (NK-cells) was determined in a 4-hour 51Cr-release test using K-562 as target cells in patients with brain tumours and donors. This activity was significantly (P less than 0.05) reduced in preoperative patients with metastatic brain tumours (mean--5.7%), gliomas of III-IV malignancy grades (mean--16.4%) as compared with the control (mean--32.5%). The activity of NK-cells remained low after surgical treatment irrespective of the length of remission.     

老年危重患者压疮的物理治疗研究

目的:探讨紫外线等物理因子联合应用,提高老年危重患者压疮的治愈率问题。方法:对我院6年来住院的281例老年压疮患者的理疗诊治记录进行回顾性总结,对其临床症状及诊治结果进行列表统计分析。结果:281例老年压疮患者,患疾病种类多,用药复杂,紫外线等物理因子联合应用,治愈率达96.4%。结论:紫外线等物理因子合理应用,治疗、控制老年压疮效果显著。     

The effect of preventive maintenance on a system with imperfect switchover

A two-unit standby redundant system with repair, preventive maintenance and imperfect switchover is considered. The Laplace-Stieltjes transform (LST) of the survivor function (sf) of the time to the first system failure (TFSF) and the mean are derived. Finally a theorem about the effect of preventive maintenance is proved.     

低强度激光照射对吸毒者血液流变学指标的影响

本文采用低强度激光血液疗法对先经强制戒毒后具有稽延性戒毒症状者进行照射治疗,并将实验组与对照组比较．观察治疗前后流变学部分指标的差异。结果表明：治疗组的两种疗法均有改善部分血液流变学指标的作用。它们虽有差异,但在统计学上无显著差异（P值均〉0．5）。激光血液疗法戒毒在治疗过程中效果好于常规戒毒,且对患者无副作用。