23例感染期糖尿病足疗效分析

目的:探讨创面负压封闭引流(VSD)技术对感染期糖尿病足治疗的应用。方法:回顾分析治疗并获得回访的感染期糖尿病足患者23例的临床资料,诸病例均行:糖尿病饮食,胰岛素严格控制血糖,严格抗感染治疗后进行感染病灶清除术并创面负压封闭引流(VSD)后行二期手术修复创面(局部皮瓣转移或逆行岛状皮瓣修复创面或自体中厚游离皮片移植修复创面)。结果:随访2~3年,治愈15例,有效7例,1例患者截肢。结论:负压封闭引流技术(VSD)是治疗感染期糖尿病足效果好、降低截肢率的较佳治疗方法。     

常规换药与负压封闭引流在糖尿病足感染期治疗中的疗效对比

目的 讨论糖尿病足患者在感染期创面的常规换药与负压封闭引流治疗疗效.方法 选择诊断为糖尿病足感染期患者共36例,分为常规换药治疗及负压封闭引流两组,观察创面在治疗5d后细菌的清除率及创面最终愈合的时间.结果 负压封闭引流组在细菌的清除率和创面最终愈合时间上明显优于常规换药治疗组（P〈0.01）.结论 在熟练掌握负压封闭引流技术的前提下,负压封闭引流技术在糖尿病足的临床治疗中值得推广应用.     

改良负压封闭引流联合中药化腐生肌法在慢性溃疡治疗中的应用

负压封闭引流(vacuum sealing drainage,VSD)[1]是加速创面愈合及创伤修复的新方法和新技术。我科专门从事创面修复的研究,自2008年以来运用改良的负压封闭引流技术联合中药化腐生肌法治疗各种慢性溃疡、难愈性创面、褥疮及糖尿病足等近百例,取得了满意的疗效,比单独采用负压封闭     

负压封闭引流在治疗糖尿病足中的护理观察

目的:探索负压封闭引流技术治疗糖尿病足的护理要点。方法:回顾性分析2009年6月～2011年6月收治的55例糖尿病足患者,根据治疗情况分为负压封闭引流治疗组和普通换药组,比较两组的换药次数及创面愈合时间。结果:28例应用负压封闭引流技术治疗的患者换药次数少,创面愈合快,效果显著。结论:手术前后持续恒定负压吸引,密切的护理观察和引流管的护理是护理的关键环节。     

负压封闭引流术在整形外科中的拓展性应用

目的 本研究探讨负压封闭引流技术（vacuum sealing drainage,VSD）在整形外科中多种复杂创面上的拓展性运用及辅助治疗效果,为临床上负压封闭引流技术在整形外科多种创面的治疗推广提供参考和依据。方法 通过回顾性分析负压封闭引流技术拓展性运用于笔者科室56例各种复杂创面的治疗,其中临近肌瓣修复胫骨外露创面17例,足部恶性黑色素瘤扩大切除加患侧腹股沟淋巴结清扫术后创面17例,感染清创术后创面12例,糖尿病足难愈性创面10例。根据具体病情特点,分别在肌瓣上一期植皮、腹股沟淋巴结清扫术后、感染创面清创后、糖尿病足清创后安置VSD,根据VSD冲洗或引流情况放置时间约7~14天后拆除,中间如有VSD堵管不畅,处理后负压不理想,可根据情况予以更换。结果 将VSD拓展于上述创面治疗后,均达到理想效果。结论 VSD能有效引流创面渗液,促进微循环、增加创面血流量,提供创面床准备、提高创面抗感染能力及刺激肉芽组织生长、封闭断端淋巴管;拓展性运用VSD于肌瓣修复创面一期植皮、腹股沟清扫预防淋巴漏、控制创面感染及糖尿病足,具有明显治疗效果;在整形外科中,VSD对复杂创面的拓展性应用具有临床推广价值。     

负压封闭引流技术治疗糖尿病足溃疡的临床效果分析

目的探讨负压封闭引流技术治疗糖尿病足溃疡的临床疗效。方法以我院收治的100例糖尿病足溃疡患者为研究对象,随机分为观察组与对照组,所有患者均进行控制血糖及清创处理,观察组使用负压封闭引流技术对创面进行治疗。比较两组患者临床治疗效果和创面愈合速度之间的差异。结果观察组患者临床治疗效果优于对照组,创面愈合速度大于对照组,其差异皆有统计学意义(P<0.05)。结论负压封闭引流技术在糖尿病足溃疡的治疗中能改善临床症状,促进创面愈合,值得临床推广应用。     

负压封闭引流(VSD)技术在糖尿病足中的临床应用

目的探讨负压封闭引流（VSD）技术在糖尿病足感染坏死创面中的临床应用。方法选取2008年12月-2012年12月共28例糖尿病足感染坏死创面中的VSD技术应用情况,在全身治疗,纠正高血糖的前提下,对创面扩创并行VSD负压引流,待创面新鲜后行植皮或皮瓣修复等关闭创面。结果创面愈合好且病程缩短,最大限度地保留了肢体长度,根据患者病程恢复顺利情况及最终个人体验结果。20例效果优,8例效果良,差0例。结论 VSD技术应用于糖尿病足坏死感染创面疗效确切,值得推广。     

VSD负压封闭引流技术对糖尿病足84例诊治分析

目的：探讨糖尿病足的临床诊断方法,观察并分析VSD负压封闭引流技术治疗糖尿病足溃疡的临床效果。方法：将我院收治的确诊为糖尿病足溃疡的84例患者作为研究对象,将其足溃疡创面进行常规清创后,利用VSD医用海绵和无菌透明密封贴膜对创面进行填补、覆盖和封闭,随后利用负压引流将创面炎性渗出物和坏死组织吸出,根据创面引流物多少及医用海绵清洁情况每3～5d更换1次VSD 医用海绵和无菌透明密封贴膜。结果：入院时糖尿病足患者各代谢指标控制达标率均较低,没有受到良好的控制,在VSD负压封闭引流治疗下,51例患者溃疡面直接愈合,13例于VSD置入后,二期植皮痊愈,20例经二期局部皮瓣转移术后治愈。结论：VSD负压封闭引流技术加快糖尿病足溃疡面愈合速度。     

负压封闭引流(VSD)技术在治疗糖尿足溃疡中的应用体会

目的:探讨负压封闭引流(VSD)技术在治疗糖尿病足溃疡中的应用,促进了创面的愈合,缩短了治疗时间.方法:46例糖尿病足患者,控制血糖平稳后,清除坏死组织,行负压封闭引流术.结果:46例糖尿病足患者行VSD治疗后创面肉芽组织生长良好,18例溃疡通过换药后创面愈合,22例经游离皮片移植或皮瓣术后创面愈合. 结论:负压封闭引流技术的使用加速促进了创面愈合,缩短治疗时间,减轻了患者因每次创面换药带来的痛苦.     

负压封闭技术的治疗进展

负压封闭技术是一种治疗急、慢性创伤创面和(或)创腔的新疗法,包括负压封闭引流技术和封闭负压辅助闭合技术,该项疗法能促进创面肉芽生长,使创面或创腔达到持续封闭的有效引流,促进炎症消退,有利于大创面的二期修复,因此负压封闭技术在临床中得到越来越广泛的应用,现就其在伤口治疗中的机制及临床应用进行综述。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.