Effects of Ang-( 1-7 ) on the apoptosis of cultured endothelial cells induced by Ang Ⅱ

Objective To investigate the effect of Ang-(1-7) on the apoptosis in human umbilical vein endothelial cells (HUVECs) induced by Ang Ⅱ.Methods HUVECs were isolated and cultured.Cultured HUVECs were incubated for 24 h with Ang-(1-7), Ang Ⅱ, Ang-(1-7) A-779, Ang-(1-7) + Ang Ⅱ, A-779 + Ang Ⅱ + Ang-( 1-7), respectively.Cultured HUVECs without incubating stimulator were chosen as controls.The apoptosis of endothelial cells were detected by flow cytometry.Results The apoptosis of endothelial cells in HUVECs were upregulated by AngⅡ ( 10-6 mol/L) (25.60% ±3.17% vs 2.32% ±0.24%, P ＜0.005).Compared with the AngⅡ group, Ang-(1-7) dose-dependently inhibited the apoptosis of endothelial cells in HUVECs ( (20.04% ± 2.21% ,16.04% ± 1.32 %, 10.04% ±2.05,7.79% ±1.50% vs AngⅡ group 25.60% ±3.17%, P ＜0.05 , P ＜0.05).The effects of Ang-(1-7) could be blocked by A-779 (23.37% ±0.75% vs 20.04% ± 2.21%, 16.04% ± 1.32,10.04% ± 2.05% ,7.79%± 1.50%, P ＜ 0.05 ).Conclusion Ang-(1-7) can attenuate the apoptosis of endothelial cells induced by Ang Ⅱ in HUVECs in a dose-dependent manner.The effects of Ang-(1-7) could be blocked by A-779( P＜0.05).     

血管紧张素－（1—7）对血管紧张素Ⅱ诱导人脐静脉内皮细胞凋亡的影响

目的 观察血管紧张素（1-7）[Ang-（1-7）]对血管紧张素Ⅱ（AngⅡ）诱导人脐静脉内皮细胞（HUVECs）凋亡的影响.方法 采用胰蛋白酶消化法原代培养HUVECs,取2～5代用于实验,培养的HUVECs随机分为：对照组、AngⅡ组、Ang-（1-7）组、AngⅡ＋Ang-（1-7）组、AngⅡ＋Ang-（1-7）＋A-779组,用吖啶橙（AO）/溴乙锭（BE）法观察细胞凋亡的形态学变化,用流式细胞术检测内皮细胞的凋亡率.结果 （1）AngⅡ（10-6 mol/L）可以诱导HUVECs凋亡率明显增加,与对照组相比差异有统计学意义（25.60%±3.17% vs 2.32%±0.24%,P＜0.005）;不同浓度的Ang-（1-7）（10-9～10-6 mol/L）呈剂量依赖性抑制AngⅡ所诱导的内皮细胞的凋亡,与AngⅡ组相比差异有统计学意义（20.04%±2.21%,16.04%±1.32%,10.04%±2.05%,7.79%±1.50%,P＜0.05）;力Ⅱ用Ang-（1-7）特异性受体拮抗剂A-779可阻断Ang-（1-7）的上述效应,与AngⅡ＋Ang-（1-7）组比较差异有统计学意义（23.37%±0.75%vs 20.04%±2.21%,16.04%±1.32%,10.04%±2.05%,7.79%±1.50%,P＜0.05）.结论 AngⅡ可诱导内皮细胞凋亡增加,Ang-（1-7）呈浓度依赖性抑制AngⅡ的上述效应,并且是通过其特异性受体Mas发挥作用.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

Caveolin-1过表达抑制宫颈鳞癌Hela细胞的体外生长研究

Objective To investigate the effects of caveolin-1 overexpressing on the growth of cervical squamous cell cancer Hela cell line. Methods Eukaryotic expression vector of human caveolin-1 gene was introduced into Hela cells by Lipofectamine. The clones stably overexpressed caveolin-1 were identiffed by RT-PCR, immunofluorescence cell staining techniques and Westernblotting. Cells proliferation viabihty was tested by MTT assay, and flow cytometry was used to assay the cell cycle and apoptosis, and the relative phosphorylation level of extracellular regulated protein kinases (Erk1/2) were detected by Westernblotting. Results The clones stably overexpressed caveolin-1 were obtained. Compared with the parental Hela cells, the tranfected cells exhibited a slower rate of growth. FAGS analysis results revealed that overexpression of caveolin-1 resulted in the cell cycle arrest in the G0/G1 [ ( 68. 04 ± 2. 57 ) % vs ( 53.41 ±1.01)%] phase and increased the apoptotic cell fraction[ (19. 18 ±2.20)% vs (5.63 ±0.55)%, P <0. 05 ]. Western blotting results showed that overexpression of caveolin-1 reduced the phosphorylation of Erk1/2(0.28 ±0.05 vs 0.81 ±0.07, P <0.05). Conclusions Overexpression of caveolin-1 suppressed the growth of Hela cells and induced apoptosis, down-regulation of Erk1/2 phosphorylation might be involved in its mechanism.     

急性淋巴细胞白血病患儿骨髓间充质干细胞对K562细胞株药物耐受性的影响

Objective To study the effect of acute lymphoblastic leukemia (ALL) children bone marrow mesenchymal stem cells (MSCs) on the resistance of K562cell atd mechanism in vitro.Method MSCs were obtained from AL children bone marrow after derivation, cultivation and identification.The coculture of MSCs and K562 and K562 suspension were established.Effects of MSCs on the growth of K562 cells were investigated in vivo.The two kinds of cells treated with different concentration of adriamycin (ADM) and the rate of apoptosis was evaluated by flow cytometry.Cell cycle was determined by flow cytometry.RT-PCR was used to detect Bcl-2 and Bax in K562 cells.Result Compared with the cell growth curve of K562 alone, the K562 cell co-cultured with MSCs grew slower and the exponential phase of growth was not obvious.The apoptosis index of the K562 cells co- clutured with MSCs was (9.19 ±0.53)% examined by flow cytometry, and that of the K562 cells alone was 4.00 ± 0.37% respectively( P ＜ 0.05 ).The percentage of cells at G0/G1 phase was (50.2 ± 2.26) % and that at S phase was (37.03 ± 3.50) % in the group of K562 alone, but those of the K562 cells co - cultured with MSCs were (80.95 ± 3.83) % and ( 17.40 ± 1.50)% respectively( P ＜0.05).The result of RT-PCR suggested expression of Bcl-2/Bax of the K562 cell co-cultured with MSCs was higher than K562 alone.Conclusion ALL children MSCs suppressed the growth of K562 cell in vitro.Adhesion made K562 depress sensitive to ADM.The mechanism was perhaps caused by adhesion with MSCs, K562 cell cycle was changed and related to Bcl-2 gene high level expression.     

急性淋巴细胞白血病患儿骨髓间充质干细胞对K562细胞株药物耐受性的影响

Objective To study the effect of acute lymphoblastic leukemia (ALL) children bone marrow mesenchymal stem cells (MSCs) on the resistance of K562cell atd mechanism in vitro.Method MSCs were obtained from AL children bone marrow after derivation, cultivation and identification.The coculture of MSCs and K562 and K562 suspension were established.Effects of MSCs on the growth of K562 cells were investigated in vivo.The two kinds of cells treated with different concentration of adriamycin (ADM) and the rate of apoptosis was evaluated by flow cytometry.Cell cycle was determined by flow cytometry.RT-PCR was used to detect Bcl-2 and Bax in K562 cells.Result Compared with the cell growth curve of K562 alone, the K562 cell co-cultured with MSCs grew slower and the exponential phase of growth was not obvious.The apoptosis index of the K562 cells co- clutured with MSCs was (9.19 ±0.53)% examined by flow cytometry, and that of the K562 cells alone was 4.00 ± 0.37% respectively( P ＜ 0.05 ).The percentage of cells at G0/G1 phase was (50.2 ± 2.26) % and that at S phase was (37.03 ± 3.50) % in the group of K562 alone, but those of the K562 cells co - cultured with MSCs were (80.95 ± 3.83) % and ( 17.40 ± 1.50)% respectively( P ＜0.05).The result of RT-PCR suggested expression of Bcl-2/Bax of the K562 cell co-cultured with MSCs was higher than K562 alone.Conclusion ALL children MSCs suppressed the growth of K562 cell in vitro.Adhesion made K562 depress sensitive to ADM.The mechanism was perhaps caused by adhesion with MSCs, K562 cell cycle was changed and related to Bcl-2 gene high level expression.     

脓毒症血清对大鼠小肠微血管内皮细胞ICAM-1的影响及肾上腺髓质素的干预作用

Objective To observe the levels of intercellar adhesion molecule-1(ICAM-1)of rat intestinal mucosa microvascular endothelial cells in sepsis rat,and to investigate the possible protective effect of adrenomedullin on the expression of intercellar adhesion molecule-1(ICAM-1)of rat intestinal mucosa microvascular endothelial cells,and to confirm whether adrenomedullin can decrease released inflammatory factors to slow down or inhibit the occurrence and development of sepsis.Methods The rat intestinal mucosa microvascular endothelial cells were isolated and cultured in vitro,and it was divided into sham operated(sham operated serum),sepsis serum(with addition of sepsis serum)and ADM serum(with 0.lng/kg adrenomedullin 6h after sepsis treatment)groups.The soluble ICAM-1(sICAM-1)content in RIMMVECs culture supernatant was measured by ELISA at the 24th hour after serum stimulation.Results The ICAM-1 content in the supernatant of sepsis group [(0.33 ±0.04)ng/L] was obviously higher than that in sham operated [(0.15 ± 0.02)ng/L] and ADM [(0.17 ± 0.04)ng/L] group(P ＜ 0.05),but ADM group was no significantly different compared with sham operated group(P ＞ 0.05).Conclusions ADM could decrease the expression of ICAM-I in RIMMVECs,and the low level of ICAM-1 could suppress its downstream inflammation factors and inhibit the occurrence and development of sepsis.     

血管紧张素-(1-7)抑制血管紧张素Ⅱ诱导的大鼠系膜细胞增殖

目的 探讨血管紧张素-(1-7)[Ang-(1-7)]对血管紧张素Ⅱ(AngⅡ)诱导大鼠系膜细胞(GMC)增殖的影响。方法 在AngⅡ诱导培养的GMC中，应用Ang-(1-7)，通过[^3H]-Thymidine及[^3H]-Leucine掺入分别测定GMC的DNA、蛋白质合成；结晶紫染色检测细胞数目，观察系膜细胞增殖情况；分别用特异性AngⅡ受体1(AT1受体)拮抗剂[Sar^1，Ⅱe^8]-AngⅡ和血管紧张素Ⅱ受体2(AT2受体)拮抗剂PD123319与Ang-(1-7)共同培养，探讨Ang-(1-7)是否通过AngⅡ受体发挥作用。结果 Ang-(1-7)呈剂量依赖性抑制AngⅡ诱导GMC的DNA、蛋白质合成及细胞数目增加；[Sar^1，Ⅱe^8]-AngⅡ和PD123319不影响Ang-(1-7)的上述作用。结论 Ang-(-1-7)能抑制基础和AngⅡ诱导的GMC增殖，其作用的发挥不通过AngⅡ的AT1和AT2受体介导。     

来曲唑对子宫内膜异位症模型大鼠的作用及对生殖系统影响的研究

Objection To study the effect of letrozole on EM rat models and influence on the reproductive system. Methods Surgically transplanted autologous uterine tissues to ectopic site beside the uterines in rats were used as animal models to study endometriosis. 20 EM model rats were random divided into letrozole-treated group and saline solution-treated control group. The change of ectopic lesion volume in each group was compared before and after treatment. Apoptotic cells were assessed by the terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick-end labeling (TUNEL) assay, and the uterian horn and ovary were weighted and observed by optical microscopy to study the change of morphology. Results The volumes of the endometriotic tissues of letrozole group reduced more than that in control group[ ( 28. 75 ± 2.28 )mm3 vs ( 108.39 ±9. 98)mm3, P <0.01 ]. The apoptotic rate in letrozole group [ (5.52 ±2. 81 )% ]was higher than control group[ (2.11 ± 1.70)%, P <0. 01 ]. The ovarian weights in letrozole-treated group increased significantly[ (25.25 ± 9. 89) mg/100g vs ( 13. 10 ± 2. 70 ) mg/100g, P < 0. 01 ], arid the ovaries showed polycyst. The uterian weights in letrozole-treated group[ (41.46 ± 15.81 ) mg/100g vs (94. 81 ±18.00) mg/100g, P <0. 05 ] significantly decreased, and the endometriums presented atrophy. Conclusion Letrozole treated EM by means of increasing the apoptosis of the ectopic tissues. Letrozole would give ovarian over stimulating and the uterian weighting decreased as well as endometriums atrophy.     

三氧化二砷联用华蟾素对K562细胞增殖和凋亡的影响

Objective To investigate the effect of As2 O3 in combination with cinobufacini on proliferation and apoptosis of the K562 cells and provide theoretical basis for clinical application.Methods Cell proliferation was assayed by analyzing the growth and viability of the cells.Apoptosis was assayed by performing cell morphology,Annexin-V/PI staining,DNA-PI staining,and DNA gel electrophoresis.Results After exposure to As2O3 and cinobufacini,the growth of K562 cells was inhibited and the viability of K562 cells was decreased. After treated with 1.0μmol/L As2O3,0.125μg/ml cinobufacini,0.25μg/ml cinobufacini,1.0μmol/L As2O3 + 0.125 μg/ml cinobufacini,1.0μmol/L As2O3 + 0.25μg/ml cinobufacini for 24 and 48 hours,the proliferation inhibition rate were(24 ± 1.3)%,(21 ± 1.5)%,(38 ± 3.1)%,(57 ±2.7)%,(66 ±3.3)% and(49 ±2.9)%,(48 ±2.7)%,(61 ±2.1)%,(77 ±3.8)%,(82 ±4.2)%,the apoptosis rate of K562 cells were(4.8 ± 0.5)%,(5.6 ± 0.7)%,(9.8 ± 0.6)%,(11.9 ± 1.2)%,(15.2±1.5)% and(11.0 ±0.9)%,(12.9 ±1.1)%,(18.4 ±1.5)%,(21.0 ±2.0)%,(28.0 ±1.9)%.The percentage of apoptotic cells was a time- and dose-dependent manner.Typical DNA ladder was shown by DNA gel electrophoresis.Conclusions As2O3 combined with cinobufacini inhibited the proliferation of K562 cells and induced apoptosis of the K562 cells,the combination of the two drugs had better effect.     

传染性单核细胞增多症患儿CD3+CD4-CD8-T细胞与T淋巴细胞亚群变化情况及临床意义

Objective To study on the changes of the DNT cells and T lymphocyte subtype in children with infectious mononucleosis (IM) and its clinical significance.Methods The flow cytometry (FCM) was used to detected the DNT cells and other T lymphocyte subtype in 48 cases of IM.Results The study showed that DNT cells( 9.39 ± 4.89 )% were greatly increased in comparison with normal controls (NC) (4.26 ± 1.68)% ( P ＜0.01 ).CD4 cells(21.45 ±9.87)% were decreased ( P ＜0.01 ) and CD8 cells increased in comparison with NC (32.43 ± 5.07) % ( P ＜ 0.01 ).Conclusion DNT cells and T lymphocyte subtype can be used to evaluate the immune function of children with infectious mononucleosis (IM) and provide guidance for adoptive immunotherapy.