γ-干扰素释放试验在淋巴结结核病中的诊断价值

目的对213例淋巴结病变患者进行γ-干扰素释放酶联免疫试验(TB-IGRA),探讨其在淋巴结结核病诊断中的临床价值。方法收集2015年10月至2016年4月就诊的淋巴结病变患者213例,其中确诊为淋巴结结核132例,非结核病的对照组81例,均进行TB-IGRA试验,并与传统的血清腺苷脱氨酶(ADA)和结核抗体相比较,分析其对淋巴结结核病诊断的灵敏度和特异性。结果TB-IGRA的敏感度87.8%、特异性70.4%,结核抗体检测的灵敏度68.2%、特异性66.7%,血清ADA的灵敏度5.3%、特异性76.5%。结论TB-IGRA试验在淋巴结结核病的诊断中具有相对较高的敏感度,可用于临床上淋巴结结核病的筛查。     

抗原诱发的γ干扰素测定在结核诊断中的应用

目的 探讨结核分枝杆菌特异性抗原早期分泌抗原靶6000(ESAT-6)体外诱发的γ干扰素反应在结核感染和结核病诊断中的价值.方法 外周全血中加入ESAT-6抗原,诱导产生γ干扰素,以酶联免疫吸附法测定γ干扰素浓度,以增加的百分率为ESAT-6抗原诱发的γ干扰素反应值.进行下列研究:对比结核密切接触者(60例)、痰培养证实的结核患者(46例)以及健康人(55例);追踪调查儿童(68例)、青少年(52例)、老年(45例);治疗过程中动态观察20例结核患者;比较HIV(+)TB(+)者(78例)和HIV(+)TB(-)者(60例).结果 结核密切接触者与痰培养证实的结核患者ESAT-6反应具有很高的敏感性;儿童与青少年ESAT-6反应与临床诊断和预后具有很高的一致性;65%的结核患者在治疗过程中ESAT-6反应呈下降趋势;ESAT-6反应在HIV(+)TB(+)的诊断中敏感度为87.2%.结论 体外ESAT-6诱发的γ干扰素测定对活动性结核病尤其是儿童和青少年结核病的诊断有一定意义,也可用于鉴别HIV阳性者合并结核.     

抗原诱发的γ干扰素测定在结核诊断中的应用

Objective To evaluate the value of early secreted antigen terget 6000(ESAT-6)-induced interferon-gamma(IFN-γ)response in vitro in diagnosis of tuberculosis and mycobacterium tuberculosis infections.Methods ESAT-6 was added into peripheral whole blood to induce IFN-γ,and concentration of IFN-γ was detected by ELISA.Comparing with saline control,the percentage of increase was regarded as the value of ESAT-6 induced IFN-γ response.ESAT-6-induced IFN-γ response was analysed in the following four aspects:to compare with the people intimate contact to tuberculosis(60 cases),the sputum culture confirmed active tuberculosis patients(46 eases)and healthy people(55 cases);to compare with children(68 cases),adolescents(52 cases)and elder people(45 cases)by follow up;dynamically observe 20 tuberculosis patients during long-time treatment;to compare HIV(+)and TB(+)people(78 cases)with HIV(+)and TB(-)people(60 cases).Results Between people intimate contacts to tuberculosis and active tuberculosis patients,ESAT-6 response has high sensitivity;ESAT-6 response in children and adolescents was completely consistent with the following clinical diagnosis and prognosis;ESAT-6 response decreased during treatment duration in 65%tuberculosis patients;in HIV(+)and TB(+)cases,the sensitivity of tuberculosis diagnosis by ESAT-6 response was 87.2%.Conclusion In vitro IFN-γ response induced by ESAT-6 is helpful in diagnosis of tuberculosis,especially for children and adolescents.And it is also available for differential diagnosis of HIV(+)patients with tuberculosis.     

抗原诱发的γ干扰素测定在结核诊断中的应用

Objective To evaluate the value of early secreted antigen terget 6000(ESAT-6)-induced interferon-gamma(IFN-γ)response in vitro in diagnosis of tuberculosis and mycobacterium tuberculosis infections.Methods ESAT-6 was added into peripheral whole blood to induce IFN-γ,and concentration of IFN-γ was detected by ELISA.Comparing with saline control,the percentage of increase was regarded as the value of ESAT-6 induced IFN-γ response.ESAT-6-induced IFN-γ response was analysed in the following four aspects:to compare with the people intimate contact to tuberculosis(60 cases),the sputum culture confirmed active tuberculosis patients(46 eases)and healthy people(55 cases);to compare with children(68 cases),adolescents(52 cases)and elder people(45 cases)by follow up;dynamically observe 20 tuberculosis patients during long-time treatment;to compare HIV(+)and TB(+)people(78 cases)with HIV(+)and TB(-)people(60 cases).Results Between people intimate contacts to tuberculosis and active tuberculosis patients,ESAT-6 response has high sensitivity;ESAT-6 response in children and adolescents was completely consistent with the following clinical diagnosis and prognosis;ESAT-6 response decreased during treatment duration in 65%tuberculosis patients;in HIV(+)and TB(+)cases,the sensitivity of tuberculosis diagnosis by ESAT-6 response was 87.2%.Conclusion In vitro IFN-γ response induced by ESAT-6 is helpful in diagnosis of tuberculosis,especially for children and adolescents.And it is also available for differential diagnosis of HIV(+)patients with tuberculosis.     

抗原诱发的γ干扰素测定在结核诊断中的应用

Objective To evaluate the value of early secreted antigen terget 6000(ESAT-6)-induced interferon-gamma(IFN-γ)response in vitro in diagnosis of tuberculosis and mycobacterium tuberculosis infections.Methods ESAT-6 was added into peripheral whole blood to induce IFN-γ,and concentration of IFN-γ was detected by ELISA.Comparing with saline control,the percentage of increase was regarded as the value of ESAT-6 induced IFN-γ response.ESAT-6-induced IFN-γ response was analysed in the following four aspects:to compare with the people intimate contact to tuberculosis(60 cases),the sputum culture confirmed active tuberculosis patients(46 eases)and healthy people(55 cases);to compare with children(68 cases),adolescents(52 cases)and elder people(45 cases)by follow up;dynamically observe 20 tuberculosis patients during long-time treatment;to compare HIV(+)and TB(+)people(78 cases)with HIV(+)and TB(-)people(60 cases).Results Between people intimate contacts to tuberculosis and active tuberculosis patients,ESAT-6 response has high sensitivity;ESAT-6 response in children and adolescents was completely consistent with the following clinical diagnosis and prognosis;ESAT-6 response decreased during treatment duration in 65%tuberculosis patients;in HIV(+)and TB(+)cases,the sensitivity of tuberculosis diagnosis by ESAT-6 response was 87.2%.Conclusion In vitro IFN-γ response induced by ESAT-6 is helpful in diagnosis of tuberculosis,especially for children and adolescents.And it is also available for differential diagnosis of HIV(+)patients with tuberculosis.     

抗原诱发的γ干扰素测定在结核诊断中的应用

Objective To evaluate the value of early secreted antigen terget 6000(ESAT-6)-induced interferon-gamma(IFN-γ)response in vitro in diagnosis of tuberculosis and mycobacterium tuberculosis infections.Methods ESAT-6 was added into peripheral whole blood to induce IFN-γ,and concentration of IFN-γ was detected by ELISA.Comparing with saline control,the percentage of increase was regarded as the value of ESAT-6 induced IFN-γ response.ESAT-6-induced IFN-γ response was analysed in the following four aspects:to compare with the people intimate contact to tuberculosis(60 cases),the sputum culture confirmed active tuberculosis patients(46 eases)and healthy people(55 cases);to compare with children(68 cases),adolescents(52 cases)and elder people(45 cases)by follow up;dynamically observe 20 tuberculosis patients during long-time treatment;to compare HIV(+)and TB(+)people(78 cases)with HIV(+)and TB(-)people(60 cases).Results Between people intimate contacts to tuberculosis and active tuberculosis patients,ESAT-6 response has high sensitivity;ESAT-6 response in children and adolescents was completely consistent with the following clinical diagnosis and prognosis;ESAT-6 response decreased during treatment duration in 65%tuberculosis patients;in HIV(+)and TB(+)cases,the sensitivity of tuberculosis diagnosis by ESAT-6 response was 87.2%.Conclusion In vitro IFN-γ response induced by ESAT-6 is helpful in diagnosis of tuberculosis,especially for children and adolescents.And it is also available for differential diagnosis of HIV(+)patients with tuberculosis.     

抗原诱发的γ干扰素测定在结核诊断中的应用

Objective To evaluate the value of early secreted antigen terget 6000(ESAT-6)-induced interferon-gamma(IFN-γ)response in vitro in diagnosis of tuberculosis and mycobacterium tuberculosis infections.Methods ESAT-6 was added into peripheral whole blood to induce IFN-γ,and concentration of IFN-γ was detected by ELISA.Comparing with saline control,the percentage of increase was regarded as the value of ESAT-6 induced IFN-γ response.ESAT-6-induced IFN-γ response was analysed in the following four aspects:to compare with the people intimate contact to tuberculosis(60 cases),the sputum culture confirmed active tuberculosis patients(46 eases)and healthy people(55 cases);to compare with children(68 cases),adolescents(52 cases)and elder people(45 cases)by follow up;dynamically observe 20 tuberculosis patients during long-time treatment;to compare HIV(+)and TB(+)people(78 cases)with HIV(+)and TB(-)people(60 cases).Results Between people intimate contacts to tuberculosis and active tuberculosis patients,ESAT-6 response has high sensitivity;ESAT-6 response in children and adolescents was completely consistent with the following clinical diagnosis and prognosis;ESAT-6 response decreased during treatment duration in 65%tuberculosis patients;in HIV(+)and TB(+)cases,the sensitivity of tuberculosis diagnosis by ESAT-6 response was 87.2%.Conclusion In vitro IFN-γ response induced by ESAT-6 is helpful in diagnosis of tuberculosis,especially for children and adolescents.And it is also available for differential diagnosis of HIV(+)patients with tuberculosis.     

抗原诱发的γ干扰素测定在结核诊断中的应用

Objective To evaluate the value of early secreted antigen terget 6000(ESAT-6)-induced interferon-gamma(IFN-γ)response in vitro in diagnosis of tuberculosis and mycobacterium tuberculosis infections.Methods ESAT-6 was added into peripheral whole blood to induce IFN-γ,and concentration of IFN-γ was detected by ELISA.Comparing with saline control,the percentage of increase was regarded as the value of ESAT-6 induced IFN-γ response.ESAT-6-induced IFN-γ response was analysed in the following four aspects:to compare with the people intimate contact to tuberculosis(60 cases),the sputum culture confirmed active tuberculosis patients(46 eases)and healthy people(55 cases);to compare with children(68 cases),adolescents(52 cases)and elder people(45 cases)by follow up;dynamically observe 20 tuberculosis patients during long-time treatment;to compare HIV(+)and TB(+)people(78 cases)with HIV(+)and TB(-)people(60 cases).Results Between people intimate contacts to tuberculosis and active tuberculosis patients,ESAT-6 response has high sensitivity;ESAT-6 response in children and adolescents was completely consistent with the following clinical diagnosis and prognosis;ESAT-6 response decreased during treatment duration in 65%tuberculosis patients;in HIV(+)and TB(+)cases,the sensitivity of tuberculosis diagnosis by ESAT-6 response was 87.2%.Conclusion In vitro IFN-γ response induced by ESAT-6 is helpful in diagnosis of tuberculosis,especially for children and adolescents.And it is also available for differential diagnosis of HIV(+)patients with tuberculosis.     

抗原诱发的γ干扰素测定在结核诊断中的应用

Objective To evaluate the value of early secreted antigen terget 6000(ESAT-6)-induced interferon-gamma(IFN-γ)response in vitro in diagnosis of tuberculosis and mycobacterium tuberculosis infections.Methods ESAT-6 was added into peripheral whole blood to induce IFN-γ,and concentration of IFN-γ was detected by ELISA.Comparing with saline control,the percentage of increase was regarded as the value of ESAT-6 induced IFN-γ response.ESAT-6-induced IFN-γ response was analysed in the following four aspects:to compare with the people intimate contact to tuberculosis(60 cases),the sputum culture confirmed active tuberculosis patients(46 eases)and healthy people(55 cases);to compare with children(68 cases),adolescents(52 cases)and elder people(45 cases)by follow up;dynamically observe 20 tuberculosis patients during long-time treatment;to compare HIV(+)and TB(+)people(78 cases)with HIV(+)and TB(-)people(60 cases).Results Between people intimate contacts to tuberculosis and active tuberculosis patients,ESAT-6 response has high sensitivity;ESAT-6 response in children and adolescents was completely consistent with the following clinical diagnosis and prognosis;ESAT-6 response decreased during treatment duration in 65%tuberculosis patients;in HIV(+)and TB(+)cases,the sensitivity of tuberculosis diagnosis by ESAT-6 response was 87.2%.Conclusion In vitro IFN-γ response induced by ESAT-6 is helpful in diagnosis of tuberculosis,especially for children and adolescents.And it is also available for differential diagnosis of HIV(+)patients with tuberculosis.     

抗原诱发的γ干扰素测定在结核诊断中的应用

Objective To evaluate the value of early secreted antigen terget 6000(ESAT-6)-induced interferon-gamma(IFN-γ)response in vitro in diagnosis of tuberculosis and mycobacterium tuberculosis infections.Methods ESAT-6 was added into peripheral whole blood to induce IFN-γ,and concentration of IFN-γ was detected by ELISA.Comparing with saline control,the percentage of increase was regarded as the value of ESAT-6 induced IFN-γ response.ESAT-6-induced IFN-γ response was analysed in the following four aspects:to compare with the people intimate contact to tuberculosis(60 cases),the sputum culture confirmed active tuberculosis patients(46 eases)and healthy people(55 cases);to compare with children(68 cases),adolescents(52 cases)and elder people(45 cases)by follow up;dynamically observe 20 tuberculosis patients during long-time treatment;to compare HIV(+)and TB(+)people(78 cases)with HIV(+)and TB(-)people(60 cases).Results Between people intimate contacts to tuberculosis and active tuberculosis patients,ESAT-6 response has high sensitivity;ESAT-6 response in children and adolescents was completely consistent with the following clinical diagnosis and prognosis;ESAT-6 response decreased during treatment duration in 65%tuberculosis patients;in HIV(+)and TB(+)cases,the sensitivity of tuberculosis diagnosis by ESAT-6 response was 87.2%.Conclusion In vitro IFN-γ response induced by ESAT-6 is helpful in diagnosis of tuberculosis,especially for children and adolescents.And it is also available for differential diagnosis of HIV(+)patients with tuberculosis.     

抗原诱发的γ干扰素测定在结核诊断中的应用

Objective To evaluate the value of early secreted antigen terget 6000(ESAT-6)-induced interferon-gamma(IFN-γ)response in vitro in diagnosis of tuberculosis and mycobacterium tuberculosis infections.Methods ESAT-6 was added into peripheral whole blood to induce IFN-γ,and concentration of IFN-γ was detected by ELISA.Comparing with saline control,the percentage of increase was regarded as the value of ESAT-6 induced IFN-γ response.ESAT-6-induced IFN-γ response was analysed in the following four aspects:to compare with the people intimate contact to tuberculosis(60 cases),the sputum culture confirmed active tuberculosis patients(46 eases)and healthy people(55 cases);to compare with children(68 cases),adolescents(52 cases)and elder people(45 cases)by follow up;dynamically observe 20 tuberculosis patients during long-time treatment;to compare HIV(+)and TB(+)people(78 cases)with HIV(+)and TB(-)people(60 cases).Results Between people intimate contacts to tuberculosis and active tuberculosis patients,ESAT-6 response has high sensitivity;ESAT-6 response in children and adolescents was completely consistent with the following clinical diagnosis and prognosis;ESAT-6 response decreased during treatment duration in 65%tuberculosis patients;in HIV(+)and TB(+)cases,the sensitivity of tuberculosis diagnosis by ESAT-6 response was 87.2%.Conclusion In vitro IFN-γ response induced by ESAT-6 is helpful in diagnosis of tuberculosis,especially for children and adolescents.And it is also available for differential diagnosis of HIV(+)patients with tuberculosis.     

γ-干扰素释放试验在结核病诊断中的应用

目的 探讨γ-干扰素释放试验（IGRAs）在结核病诊断中的应用价值。方法 选取2017年7月~2019年6月在景德镇市第一人民医院就诊的100例疑似结核感染患者和50例健康体检者作为研究对象,分别行γ-干扰素释放试验和涂片抗酸染色镜检。将最终确诊为结核病患者48例设为结核病组,52例非结核病患者作为非结核病组,50例健康体检者设为正常对照组。分析三组TB-IGRAs阳性率,对比肺结核病组涂阴与涂阳TB-IGRAs检测结果,并比较TB-IGRAs与涂片抗酸染色对结核病的诊断效能。结果 结核病组IGRAs阳性率高于非结核病组和正常对照组,差异有统计学意义（P＜0.05）;涂阳肺结核和涂阴肺结核患者的IGRAs阳性率比较,差异无统计学意义（P＞0.05）;IGRAs法敏感度为85.42%,特异性为89.22%,阳性预测值为78.85%,阴性预测值为92.86%;涂片抗酸染色法敏感度为22.92%,特异性为100.00%,阳性预测值为100.00%,阴性预测值为73.38%; IGRAs诊断结核病的敏感度高于涂片抗酸染色,差异有统计学意义（P＜0.05）。结论 IGRAs具有较高的敏感度和阴性预测值,对结核病的诊断具有较高的诊断价值,值得在临床中应用。     

结核分枝杆菌相关IFN-γ释放试验在结核病诊断及疗效监测中的应用

目的：目的：探讨结核分枝杆菌相关γ-干扰素释放试验（TB-IGRA）在结核病诊断及临床抗结核治疗效果评价中的应用价值。方法：对196例临床确诊结核患者进行相关病史收集,同时进行TB-IGRA、结核菌素皮肤试验、结核杆菌培养、涂片镜检、结核抗体检测,结核患者的治疗过程中按用药疗程（初治患者第2、5、6个月,复治患者第2、5、8个月）对其进行随访,在治疗结束后6个月和1年后各随访一次,比较TB-IGRA与结核菌素皮肤试验在治疗过程中的变化。结果：确诊结核病例196例,其中肺结核158例,肺外结核38例,TB-IGRA在结核诊断中灵敏度为82．1％,特异性为91．7％,阳性预测值为93．1％,阴性预测值为79．3％,与传统实验室检查方法相比有显著性差异（P〈0．01）。在结核患者的抗结核治疗过程中,TB-IGRA阳性率及浓度水平治疗前与治疗后2、6月以及复治后2、5、8月比较显著下降（P〈0．01）,TB—IGRA水平与结核分枝杆菌数量存在较好相关性,与传统的TST方法评估疗效相比有显著性差异（P〈0．01）。结论：TB-IGRA在结核诊断中敏感性和阳性预测值较高,可作为较好的结核诊断的依据,同时TB-IGRA对肺外结核的灵敏度较高,为肺外结核患者的诊断提供了较为可靠的依据,通过治疗过程TB—IGRA检测分析,外周血TB-IGRA的变化随着抗结核治疗的进程下降的趋势明显,与结核涂片镜检结果符合率较高,是临床疗效评估较为理想的指标。     

结核感染T细胞斑点试验在结核诊断与筛查中的价值

目的 探讨结核感染T细胞斑点试验(T-SPOT.TB)在结核诊断与筛查中的价值.方法 对2013年1月至2016年12月在南通市第一人民医院就诊的266个患者分为结核组、非结核组及生物制剂组,抽取静脉血应用结核感染T细胞斑点试验进行检测,同时部分患者进行了结核菌素检测.结果 T-SPOT.TB法诊断TB患者的敏感度为87.9％,阳性预测价值和阴性预测价值分别是43.2％和88.5％,TB组T-SPOT.TB检测阳性率明显高于非TB组(P ＜0.001)和生物制剂组(P＜0.001)的阳性率.而TST试验结果中,TB组阳性率与非TB组、非TB组阳性率与生物制剂组差异均没有统计学意义(P＞0.05),而TB组阳性率与生物制剂组差异有统计学意义(P＜0.05).在所有受试者T-SPOT.TB检测和TST试验的一致性为70.3％(K =0.397),生物制剂组中两者一致性为59.5％ (K=0.313).在TB组和非TB组中,两种方法一致性更高,分别为83.7％(K=0.486)和77.1％ (K=0.437).T-SPOT.TB法中TB组的斑点数显著高于非TB组(P ＜0.001)和生物制剂组(P＜0.001),而非TB组和生物制剂组的斑点数差异没有统计学意义(P＞0.05).结论 T-SPOT.TB检测比结核菌素试验有更高的灵敏度及阴性预测价值,并且不受环境分枝杆菌和BCG接种的影响,使得T-SPOT.TB在结核感染的诊断与筛查中具有较好的临床应用价值.     

恒温扩增检测结核分枝杆菌特异性IS6110片段在肺结核检测中的应用

目的 比较恒温扩增技术检测、抗酸杆菌涂片、结核分枝杆菌培养在肺结核患者检测中的应用.方法 收集2016年1月1日至12月31日我院呼吸科肺结核患者清晨痰液,将标本分为3份,分别进行痰涂片抗酸染色,结核菌培养及恒温扩增检测结核分枝杆菌特异性IS6110片段,统计分析3种方法的检测结果.结果 56例痰标本,恒温扩增检测结核分枝杆菌特异性IS6110片段阳性33例,阳性率为58.93％,抗酸染色阳性15例,阳性率为26.79％,恒温扩增检测结核分枝杆菌特异性IS6110片段阳性率高于抗酸染色方法;结核分枝杆菌培养阳性为35例,阳性率62.50％;恒温扩增检测结核分枝杆菌特异性IS6110片段和结核分枝杆菌培养两种方法检测Kappa系数为0.776,检测一致性较好.结论 恒温扩增检测结核分枝杆菌特异性IS6110片段是一种简便、高灵敏度的检出结核分枝杆菌的方法.     

Rv1813c在结核分枝杆菌潜伏感染诊断中的价值

目的 研究重组结核分枝杆菌潜伏感染蛋白Rv1813c在诊断结核分枝杆菌潜伏感染方面的价值.方法 20例结核潜伏感染者和79例健康志愿者均行胸部X线检查、PPD皮肤试验、结核抗体检测,同时应用ELISA联合重组融合蛋白CFP10-ESAT6和潜伏感染蛋白Rv1813c进行IGRA检测.结果 所有受试者中,PPD皮肤试验和抗体检测的阳性率分别为50％和28％.Rv1813c刺激潜伏感染者后产生IFN-γ的水平显著高于健康对照(P＜0.05),其刺激PPD强阳性组(皮试直径≥15mm)产生的IFN-γ值低于弱阳性组(5mm≤皮试直径＜15mm),但无统计学意义,而CFP10-ESAT6刺激PPD强阳性组后产生的IFN-γ值高于弱阳性组(P＜0.05).CFP10-ESAT6和Rv1813c刺激抗体阴性及阳性组后产生的IFN-γ水平没有显著性差异(P＞0.05).Rv1813c诊断结核感染的ROC曲线下面积为0.834,特异性80％时,灵敏度为85％;特异性为90％时,灵敏度为45％.结论 同时检测Rv1813c及CFP10-ESAT6抗原特异的IFN-γ值有可能筛选出潜伏感染者中的活动感染人群,对于结核分枝杆菌潜伏感染诊断有重要的应用价值.     

结核分枝杆菌潜伏感染早期两种检测方法的比较分析

目的比较结核分枝杆菌（Mycobacterium tuberculosis,MTB）特异性IFN-γ酶联免疫斑点法（Elispot）和结核菌素皮试（TB—PPD皮试）在MTB潜伏感染早期诊断的优异。方法采用自主研制的Elispot试剂盒（简称Elispot）对100例健康志愿者进行检测,并和TB—PPD皮试进行平行比较。结果100例健康志愿者中有50例TB—PPD皮试阳性（占50％）,Elispot检测阳性20例（占20％）。两种方法检测结果差异有统计学意义（x^2=20．45,P〈0．005）。结论MTB特异性IFN-γ Elispot试剂盒在健康人群中检测阳性率比传统的TB-PPD皮试要低,Elispot检测体外IFN-γ 应答反应在诊断MTB潜伏感染的价值优于TB—PPD皮试,在MTB潜伏感染早期诊断有较好的发展前景。     

肺结核患者痰中结核分枝杆菌检验的临床价值分析

目的探讨分析肺结核患者痰中结核分枝杆菌检验的临床价值遥方法选取该院近期收治的肺结核患者82 例,根据患 病性质将其分为活动组（58 例）与非活动组（24 例）,对比两组患者晨痰痰液中结核分枝杆菌培养阳性率遥结果活动组结核分 枝杆菌培养阳性率（63.79%）较非活动组高（20.83%),两组比对差异存在统计学意义（约0.05）遥结论结核分枝杆菌培养可提升 结核分枝杆菌阳性检测率,对肺结核患者早期检出以及治疗意义重大,值得推广与借鉴遥