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以紫甘薯花青素为原料,采用柠檬酸水溶液为提取剂,分别研究提取剂浓度、浸提温度、浸提时间和料液比对花青素提取率的影响。在此基础上,采用3因素3水平响应面分析法,以吸光度为响应值,探讨浸提温度、浸提时间和料液比对紫薯花青素提取的影响并对提取工艺进行优化。紫薯花青素在波长为524nm处有最大吸收峰。单因素实验证明当柠檬酸浓度为5%、浸提温度为50℃、浸提时间为4h、料液比为1∶20g/mL时花青素提取率达到最大。响应面分析证明对花青素提取率影响大小的顺序为料液比、提取温度和提取时间。响应面分析法确定紫薯花青素最佳提取工艺参数为:提取温度46℃,提取时间6h,料液比1∶23g/mL,在此条件下花青素含量为159mg/100g。 相似文献
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采用微波辐射预处理金柚皮,以乙醇作提取剂从金柚皮中提取黄酮类物质,探讨了影响金柚皮总黄酮提取的微波辐射预处理条件(辐射功率和辐射时间)、乙醇浓度、浸提时间、浸提温度、料液比因素。通过正交试验确定了最佳工艺条件。结果表明,在微波辐射功率495W、辐射时间3min 预处理金柚皮后,用80% 乙醇作为浸提剂,按料液比1:8,在温度70℃下浸提40min 可取得最佳提取效果。此条件下总黄酮提取率达0.99%。 相似文献
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该试验对菝葜根茎色素的最佳提取条件和色素的稳定性进行探究性试验。把单因素试验结果作为试验基础,以吸光度作为检测指标,通过采用L9(33)正交试验设计,验证试验对菝葜根茎色素的提取工艺条件进行最佳优化,同时探究其氧化还原剂、金属离子等因素对菝葜根茎色素稳定性的影响。结果表明,最佳提取工艺为提取温度70℃,提取液浓度50%乙醇,提取时间1.5 h,料液比为1∶10(g/mL)。稳定性试验结果表明加入氧化剂H2O2和还原剂Na2SO3对菝葜根茎色素的吸光值有上升趋势;金属离子检测时Fe3+对色素有显著影响,其他金属离子对菝葜根茎色素液的影响不明显。 相似文献
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Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Helminthosporium oxysporium, and Penicillium frequestans isolated from common Nigerian agricultural residues like cassava, yam, banana, and plantain peels and brewery spent grains
(BSG) were screened for their ability to produce β-amylase and amyloglucosidase using submerged and solid-state cultivation
regimens. Enzyme activity (EU) was determined by estimating the amount of reducing sugars produced as a result of the action
of crude enzyme solutions on buffered starch solution. Results showed that A. niger liberated the highest level of β-amylase (33.2 EU) on plantain peels medium using the static cultivation method, while it
produced the highest amount of amyloglucosidase (29.8 EU) on yam peels substrate with solid-state cultivation regimen. Plantain
peels favored β-amylase production more than the other tested wastes supporting production of 33.2 and 9.8 EU of the enzyme
in A. niger under solid-state and static cultivation methods, respectively. Yam peels favored liberation of 15.3 EU amyloglucosidase
in each of the solid-state and the static cultivation regimens. Production of β-amylase on BSG, yam, banana, and plantain
peels was not significantly facilitated by static cultivation method (p > 0.05), while solid-state cultivation regimen favored amyloglucosidase production on cassava peels and BSG (p < 0.05). Optimal production yield of β-amylase and amyloglucosidase were 16.6 and 14.9 EU g−1 on plantain and yam waste media by the mold. 相似文献
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ABSTRACT: The formation of zinc-chlorophyll-derivative complexes was investigated in peels-on green D'Anjou pears when subjected to blanching in zinc ion solution (1300, 2600, and 0 ppm) at 94 °C for 6, 12, or 18 min and then canning at 94 °C for 20 min. The peels removed from the pears were freeze-dried and ground into powders in liquid nitrogen for pigment extraction using ethyl ether. The visual absorption of the extracts was measured using a spectrophotometer along with identification and quantification of chlorophyll derivatives using reverse-phase HPLC method. Furthermore, pears with or without the peels were blanched in 2600 ppm of zinc solution for 12 min following the canning process in 10 °Brix syrup solution. Total antioxidant (TA) and total phenolic content (TPC) of the pear flesh and peels were evaluated using Folin-Ciocalteu's phenol and 1,1-diphenyl-2-picrylhydrazyl assays. Thermal processing destroyed chlorophylls on pear peels, in which pheophytins were found to be the major degraded compounds while an insignificant amount of pyropheophytins was also formed. In zinc blanched peels, Zn pheophytins a was the dominant green compound, and its amount increased about 100% and 144.4% in peels blanched in 1300 ppm zinc solution for 6 and 12 min, respectively. When blanching peels in 2600 ppm zinc solution for 6 and 12 min, the pigment increased about 118% and 242%, respectively. Significant reductions in TA and TPC were found on the peels of zinc treated pears, but the overall TA and TPC of whole fruits were not significantly affected by the treatments. 相似文献
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Yams (Dioscorea spp.) are perennial trailing rhizome plants. Steroidal saponins, furostanol and spirostanol glycosides are the marked functional compounds in yams. In this investigation, a C18 solid phase extraction method was developed for yam saponins purification. The contents of saponins in various organs of yam (Dioscorea pseudojaponica Yamamoto) were also determined. Results showed that the recoveries of yam saponins extracted by the developed method were about 99.48–100.08% when the saponins (each saponin weighed 0.20, 0.50 and 1.00 mg) passing through the C18 cartridge. The extractive method could efficiently reduce the interferences from impurities in yam saponin extracts prior to HPLC analysis. The recoveries of added saponins in different yam organs were 98.34–99.92% for tuber flesh, 95.98–98.89% for tuber cortex, 97.89–99.44% for rhizophor, 93.82–98.01% for leaf and 93.87–97.65% for vine, respectively. The yam tuber cortex had the highest amount of saponins (582.53 μg/g dw), which was higher than that existed in the tuber flesh (227.86 μg/g dw) about 2.55 times. The contents of saponins in the rhizophor, leaf and vine of yam were 29.39, 24.41 and 23.96 μg/g dw, respectively. 相似文献
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Pomegranate peels are one of the most valuable by-products of the food industry in terms of polyphenols which are conventionally extracted from plant materials by organic solvents, especially with methanol. Pressurised water extraction was investigated for the extraction of polyphenols from pomegranate peels. The most important factors affecting the extraction results were found to be particle size, temperature, and static time. The results indicated that pressurised water extraction was as effective as conventional methanol extraction for the recovery of polyphenols from pomegranate peels. Total phenolic contents of pomegranate peels obtained by pressurised water extraction at optimised conditions and conventional solid–liquid methanol extraction were determined as 264.3 and 258.2 mg/g tannic acid equivalents, respectively. Hydrolyzable tannins were the predominant polyphenols of pomegranate peels corresponding to 262.7 mg/g tannic acid equivalents. Punicalagin content of pomegranate peels by pressurised water extraction was found to be 116.6 mg/g on dry matter basis. 相似文献
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