解脲支原体感染与稽留流产的关系

目的探讨解脲支原体（UU）感染与稽留流产的关系。方法采用分离培养法,分别对96例早孕稽留流产患者（研究组）和80例早孕要求终止妊娠者（对照组）进行宫颈分泌物UU培养,并对两组清宫后绒毛及蜕膜送病理检查。结果研究组宫颈分泌物UU培养阳性例数（96例,占61.46%）明显高于对照组（13例,占16.25%）,两组比较,差异有统计学意义（P〈0.05）,研究组有52例（54.16%）发生绒毛膜炎,对照组有4例（5.0%）,两组比较,差异有统计学意义（P〈0.05）。结论解脲支原体（UU）感染可通过上行传播引起宫内感染,绒毛膜炎而导致稽留流产。     

血浆循环DNA在肺癌诊断上的应用

 目的研究血浆循环Fhit基因D3S1300位点杂合性缺失(LOH)作为肿瘤标志物在非小细胞肺癌早期诊断中的意义.方法以微卫星技术检测58例非小细胞肺癌(Non small cell lung cancer,NSCLC)患者血浆及肿瘤组织以及40例非肺癌肺科住院患者血浆Fhit基因D3S1300LOH的表达情况.结果58例NSCLC患者中肿瘤组织D3S1300 LOH检出率为41.4%(24/58),在其匹配的血浆中D3S1300 LOH检出率为29.3%(17/58),二者差异有统计学意义(P＜0.05).对照组血浆游离DNA D3S1300 LOH检出率为5%(2/40),与NSCLC组比较差异有统计学意义(P＜0.01).结论血浆循环DNA Fhit基因D3S1300 LOH是一种简单有效的肺癌肿瘤筛选标志物.     

透明质酸钠在输卵管介入再通术后预防复发性粘连的临床应用

目的 评价透明质酸钠在输卵管介入再通术后预防复发性粘连的临床价值.方法 将169例输卵管阻塞性不孕症患者随机分为对照组(60例)和观察组(109例),前者行输卵管介入再通术后常规使用松解液(庆大霉素8万u、地塞米松10 mg、α-糜蛋白酶5 g的水溶液40 ml)治疗,后者则在此基础上加用透明质酸钠预防复发性粘连.观察2组术后输卵管再通、复发性粘连发生情况及受孕情况.结果 观察组109例子宫输卵管造影显示输卵管阻塞210条,术后缺如8条,介入再通202条,再通成功率为96.2%(202/210);对照组60例经子宫输卵管造影显示输卵管阻塞108条,介入再通103条,再通成功率为95.4%(103/108),2组比较无显著性差异(P＞0.05).观察组介入再通术后3个月粘连复发率及12个月内受孕率分别为3.7%和57.8%,对照组分别为26.7%和25.0%,2组比较有显著性差异(P＜0.05).结论 透明质酸钠能有效预防输卵管介入再通术后复发性粘连的发生,具有很好的临床应用价值.     

低剂量螺旋CT对早期肺腺癌诊断价值分析

目的分析低剂量螺旋CT对早期肺腺癌的诊断价值。方法选取接受低剂量或普通剂量MSCT检查肺腺癌的患者各86例,分别作为观察组、对照组。对比分析两组的病理检查结果、肺腺癌检出率、结节检出率、图像质量、辐射相关指标等。结果病理检查结果显示,观察组86例患者中34例(39.53%)为早期肺腺癌,对照组诊断早期肺腺癌30例(34.88%),两组无统计学差异,即P0.05。观察组低剂量CT肺腺癌检出率为32.56%(28/86),高于对照组25.58%(22/86),但差异不具有统计学意义,即P0.05。观察组的结节检出率为54.65%(47/86),显著高于对照组37.21%(32/86),结果具有统计学差异,即P0.05。观察组图像质量较差率、良好率多于对照组,但差异均不具有统计学意义,即P0.05。观察组优秀率为65.12%(56/86),显著少于对照组82.56%(71/86),差异具有统计学意义,即P0.05。对比两组CT的DLP、CTDIw、毫安秒、放射剂量等辐射量相关指标水平可知,观察组均少于与对照组,结果有统计学意义差异,即P0.05。结论低剂量MSCT与普通剂量CT的检查结果一致,图像质量尚可,具有诊断意义,且前者辐射量低,可作为早期肺癌患者诊断的首要选择。     

静脉麻醉药在子宫输卵管造影术中的临床应用

目的探讨静脉麻醉药在子宫输卵管造影(hysterosalpingography,HSG)中的临床应用价值。方法 158例不孕患者按照数字表法随机分为研究组和对照组,两组患者均在造影后作腹腔镜通染液试验(1aparoscopy and dye test,CLP),并以CLP结果作为标准判断造影诊断的正误。结果以CLP检查结果为金标准,在静脉麻醉的情况下进行的子宫输卵管造影检查(研究组)结果与金标准CLP检查结果一致的病例为69例,而在无麻醉情况下进行的子宫输卵管造影检查(对照组)结果与金标准CLP检查结果一致的病例为66例。经统计学分析发现研究组(87.34%)和对照组(83.54%)的检查结果与CLP符合率比较无明显差异(2=0.46,P0.05)。但是,研究组在假性输卵管梗阻检出率(2.53%)方面明显低于对照组(10.13%),且差异具有统计学意义。研究组患者的疼痛评分较对照组明显下降,且差异具有统计学意义。经统计学分析发现研究组的不良反应发生率(5.06%)与对照组(2.53%)比较无明显差异(2=0.69,P0.05)。结论在静脉注射丙泊酚进行静脉麻醉的情况下实施子宫输卵管造影检查不仅可以消除患者的紧张情绪,而且可提高常规子宫输卵管造影检查的准确性。     

数字X线摄影在子宫输卵管造影中的应用及不良反应分析

目的探讨数字X线摄影在不孕症患者子宫输卵管造影中的应用价值。方法选择我院收治的不孕患者200例为研究对象,简单随机数字表法分为观察组及对照组,每组100例。对照组患者行常规子宫输卵管造影,观察组患者行数字X线摄影子宫输卵管造影。比较两组患者的图像、输卵管梗阻及不良反应情况。结果两组患者的图像质量比较,差异具有统计学意义(P<0.05),且观察组患者的射线剂量及图像噪声均小于对照组患者,差异具有统计学意义(P<0.05)。两组患者的输卵管通畅、输卵管梗阻例数比较,差异具有统计学意义(P<0.05),而输卵管通而不畅例数比较,差异无统计学意义(P>0.05)。两组患者的不良反应情况比较,差异无统计学意义(P>0.05),而观察组患者的发生率为6.00%,低于对照组患者的15.00%,差异具有统计学意义(P<0.05)。结论数字X线摄影在子宫输卵管造影中可获高质量的图像,同时还降低了X线辐射剂量及不良反应。     

64排CT诊断肠壁缺血性病变的临床应用分析

目的探讨64排CT对于诊断肠壁缺血性病变的诊断价值及临床作用。方法选取本院收治过的100例肠壁缺血性病变的患者,所有肠壁缺血性病变患者的收取时间(2016年2月1日～2017年2月10日),电脑随机分为两组,观察组一组(50例肠壁缺血性病变患者)、对照组一组(50例肠壁缺血性病变患者),分别进行64排CT扫描诊断以及普通常规CT扫描诊断,将扫描后的两组肠壁缺血性病变患者的诊断价值进行对比。结果观察组肠壁缺血性病变患者的检出率92.00%,高于对照组患者检出率70.00%(P0.05);观察组肠壁缺血性病变患者误诊率为4.00%,漏诊率为4.00%,和对照组具有明显差异(P0.05)。结论使用64排CT对肠壁缺血性病变的诊断,取得了明显效果。     

子宫输卵管造影与腹腔镜输卵管检查输卵管性不孕症的临床分析

目的探讨子宫输卵管造影(hysterosaipingography,HSG)与腹腔镜输卵管检查在诊断输卵管性不孕症中的效果。方法对68例输卵管性不孕症患者(136条输卵管)进行子宫输卵管造影与腹腔镜输卵管检查,将两种方法对输卵管通畅性的检查结果进行对比分析。结果腹腔镜下美蓝通染液试验(CLP)结果显示本组68例患者的136条输卵管中输卵管通畅84条,输卵管通而不畅26条,输卵管阻塞26条。以CLP检查结果作为对照进行对比分析发现输卵管造影在输卵管通畅性检查结果方面与CLP比较无明显差异。输卵管造影与腹腔镜输卵管在判断输卵管梗阻部位比较中差异无统计学意义(X~2=0.48,P0.05)。结论 HSG操作便捷、费用低廉,能较好的反映子宫输卵管全貌及梗阻部位,可以作为输卵管性不孕症检查的初筛;腹腔镜检查是诊断输卵管通畅性的"金标准",两者联合是诊断输卵管性不孕症的最佳方法。     

大脑中动脉高密度征探讨

目的 评价大脑中动脉高密度征(HMCAS)在早期脑梗死颅脑CT诊断中的临床价值.方法 选取17例经确诊的大脑中动脉供血区脑梗死病人作为病例组,连续选取245例无脑血管病症状及体征、颅脑CT平扫未见异常的体检者作为对照组,评价其颅脑CT平扫所见大脑中动脉密度.结果 病例组中HMCAS的检出率11.76%(2/17)高于对照组0.82%(2/245)(P<0.05),病例组DSA所见大脑中动脉血栓在颅脑CT平扫中未发现局灶性动脉致密征.结论 HMCAS与大脑中动脉供血区脑梗死有一定的关系,但颅脑CT平扫所见HMCAS与大脑中动脉血栓没有直接联系.     

输卵管性不孕症的放射介入治疗评价

目的:评价放射介入再通术对输卵管性不孕症的疗效。方法:随访102例接受选择性输卵管造影(SSG)和介入性输卵管再通术(FTR)治疗的输卵管阻塞不孕症患者,统计术后1年内妊娠率、异位妊娠率及输卵管再阻塞率,并进行综合分析。结果:完全梗阻患者1年妊娠率为40.7%,其中近端(间质部、峡部)阻塞妊娠率为60%,中远端(壶腹部、伞端)阻塞妊娠率为16.67%,两者比较差异有显著性(χ2=5.19,P<0.05);不完全阻塞患者1年妊娠率分别为通而欠畅60.9%、通而不畅43.2%、通而极不畅13.3%。三者比较差异有显著性(χ2=8.40,P<0.05)。结论:SSG和FTR是治疗输卵管性不孕症的有效方法,输卵管近端阻塞为SSG和FTR首选。     

实时荧光定量PCR法在检测泌尿生殖道感染病原体中的应用

目的评价荧光定量PCR（FQ-PCR）在实验室检测泌尿生殖道病原体、淋病奈瑟菌（NG）、解脲脲原体（UU）和沙眼衣原体（CT）中的应用。方法 464例拟诊为泌尿生殖道感染者的临床标本,用培养法检测NG、UU病原体和胶体金免疫层析法检测CT抗原,对同一标本同时用FQ-PCR检测NG、UU或CT的核酸。结果 FQ-PCR和培养法检出NG阳性率分别为28.4%（63/222）和14.9%（33/222）,P〈0.01,检测UU的阳性率分别为41.7%（126/302）和32.5%（98/302）,P〈0.05,FQ-PCR和金标法检测CT阳性率分别为18.2%（57/314）和6.1%（19/314）,P〈0.001,FQ-PCR对3种病原体的检出率均明显高于培养法或金标法。结论 FQ-PCR法检测NG、CT以及UU在临床诊断NG,UU和CT感染中具有重要应用价值,是常规方法的有效补充。     

A modular real-time PCR concept for determining the quantity and quality of human nuclear and mitochondrial DNA

We developed a modular real-time (rt) PCR system for absolute quantification of human nuclear (n) and mitochondrial (mt) DNA. For determination of the number of amplifiable template molecules with a minimum length required for downstream genotyping and assessment of the PCR-relevant degradation grade of the template DNA, primers yielding differently sized PCR products (nDNA: 79, 156, and 246 bp; mtDNA: 102, 143, 283, and 404 bp) and TaqMan hybridization probes were used for amplification and on-line product detection. DNase-degraded DNA served as model to demonstrate the effects of DNA fragmentation on rtPCR quantification and subsequent genotyping. Introduction of cloned internal amplification positive controls (IPCs) – generated by in vitro mutagenesis of primer-binding sites of the wild-type nDNA and mtDNA targets – enabled functionality-testing of the reaction mixture and detection of PCR inhibitors in DNA extracts, without a need for additional TaqMan probes. A hematin model was used to test the ability of the quantitative real-time (rtq) PCR system to predict the effects of inhibitors in downstream PCR-based genotyping.     

Infertility in women: diagnostic evaluation with hysterosalpingography and other imaging techniques.

This article examines the current roles of hysterosalpingography and other imaging studies used in diagnostic assessment and treatment of infertility in women. The advantages and limitations of hysterosalpingography, transabdominal or transvaginal sonography, and MR imaging in detecting abnormalities of the uterus (congenital anomalies, intrauterine adhesions, myometrial and endometrial derangements) and tubal and peritubal disease are presented. The use of sonography to evaluate cyclical endometrial changes, to assess development of the follicle and corpus luteum, to determine whether ovulation has occurred, and to detect dysfunctional ovulatory dynamics is described, as is the role of this technique in improving the prognostic value of the postcoital test for assessing the quality of cervical mucus. With the development and availability of newer and improved methods for the treatment of infertility disorders in women, comprehensive and detailed evaluation to determine the precise underlying cause or causes of the infertility is important to allow formulation of the most appropriate therapeutic plan. With careful and thorough assessment, the cause of infertility can be identified in 90-95% of couples evaluated.     

解脲支原体感染与宫颈糜烂的关系

目的：了解解脲支原体（Uu）感染与宫颈糜烂的关系方法：采用液体培养法对600例宫颈糜烂患者和300例健康对照者进行Uu的检测,并根据宫颈糜烂患者病变程度及病程进行分组研究。结果：宫颈糜烂患者Uu检出率为43.2%明显高于对照组7.7%（P〈0.01）,其中轻度宫颈糜烂患者检出率为6.1%,中度为21.1%,重度45.9%。各组检出率经统计学处理有显著差异（P〈0.05）;宫颈糜烂病程与Uu感染呈正相关,病程〉12月的宫颈糜烂患者的Uu感染率明显升高（P〈0.05）。结论：Uu感染在宫颈糜烂发病中起重要作用;宫颈糜烂病程越长、宫颈糜烂程度越重,Uu感染率越高。     

液基薄层细胞学和DNA定量分析方法在宫颈癌筛查中的对比分析

目的 比较采用液基薄层细胞学和DNA定量分析方法进行宫颈癌筛查的有效性.方法 对参与宫颈癌普查的2 199名妇女用宫颈刷取材,进行液基薄层制片,分别行液基薄层细胞学诊断和DNA定量分析.其中136例进一步行宫颈组织病理检查.结果 分别以≥3个DNA异倍体细胞和低级别鳞状上皮内病变及以上级别作为评估CINII及以上病理改变,其敏感度、特异度、阳性预测值、阴性预测值分别为95.83%和87.50%、72.32%和58.04%、42.59%和30.88%、98.78%和95.59%.DNA定量分析方法的敏感度、特异度、阳性预测值、阴性预测值均高于液基薄层细胞学方法.结论 DNA定量分析方法可提高宫颈癌筛查的敏感度和特异度.     

Presence of interleukin-8 and the IL-1 receptor antagonist in the cervical mucus of fertile and infertile women

BACKGROUND: Cytokines are involved in almost every aspect of reproduction, and recent studies suggested a relationship between cytokines and male/female infertility. In the present study, the levels of interleukin-8 (IL-8) and interleukin-1 receptor antagonist (IL-1Ra) were determined in the cervical mucus of fertile and infertile women. METHODS: Groups of patients were formed according to the results of the standard procedure for infertility investigation, including postcoital test and the presence of antispermatozoid antibodies in the sera of both partners and in seminal plasma, by mixed antiglobulin reaction (MAR) test and Kibrick agglutination test. IL-8 and IL-1Ra levels were determined in solubilized (ultrasonographic sonication) cervical mucus sampled in the midcycle by commercial ELISA kits and expressed as pg/mg proteins. RESULTS: The groups were designated as fertile (n=20) and infertile (n=48). The latter was divided into two subgroups, one consisting of infertile women with positive postcoital test and without antispermatozoid antibodies (n=30), and the other designated as infertile women with negative postcoital test (n=18). This subgroup was composed of women with negative postcoital test and without antibodies (n=10) and the women with negative postcoital test with antibodies (n=8). Similar levels of IL-8 and IL-1Ra were noted in the cervical mucus of infertile women and women with positive postcoital test and without antispermatozoid antibodies. A tendency of decrease (p=0.052) and significant decrease in IL-8 levels (p<0.05) was noted in negative postcoital test group and negative postcoital test group without antibodies, respectively, compared to the levels in the fertile examinees. A significant rise in IL-1Ra levels (p<0.05) was detected in the mucus of negative postcoital test group with further increase in negative postcoital test group with antibodies (p<0.02). CONCLUSION: Changes in IL-8 and IL-1Ra levels in the cervical mucus of infertile patients with negative postcoital test suggested the existence of the relationship between cervical cytokines and infertility in these women.     

激光治疗慢性宫颈炎后愈合不良与非淋菌性感染的关系

目的探讨激光治疗慢性宫颈炎后愈合不良与非淋菌性感染的关系。方法选择经激光治疗后愈合不良的慢性宫颈炎患者64例，愈合良好的患者53例，记录两组患者术前宫颈糜烂面积，激光术后检测宫颈拭子沙眼衣原体、解脲脲支原体。结果愈合不良组衣原体感染率25．0％（16／64），支原体感染率32．8％（21／64）；愈合良好组衣原体感染率3．8％（2／53），支原体感染率11．3％（6／53），两组比较差异有显著意义（P〈0．01）。宫颈感染支原体、衣原体后，宫颈呈中、重度炎症。结论非淋菌性感染影响激光治疗慢性宫颈炎的预后。     

沙眼衣原体、解脲支原体、淋病奈瑟氏菌感染状况分析

目的　探讨本地STD主要病原体的感染状况。方法　采用PCR方法检测我院门诊病人尿道和生殖道标本中沙眼衣原体 (CT)、解脲支原体 (UU)、淋病奈瑟氏菌 (Ng)。结果　男性患者CT、UU和Ng分别为 8 4 % ,9 0 %和2 2 0 % ,女性患者CT、UU和Ng感染率感染率分别为 8 2 % ,17 0 %和 8 3%。结论　本地区男女的STD主要病原体的感染谱不同 ,男性以Ng感染为主 ,女性以UU感染为主 ,UU已取代Ng成为女性STD首要病原体。     

Selective quantification of human DNA by real-time PCR of FOXP2

We established a simple quantitative PCR procedure with high specificity and sensitivity using TaqMan probes targeting the FOXP2 sequence. This assay distinguished human and nonhuman, including primates, samples with the exception of mouse, turtle, lizard, and fishes. However, the specific amplification of mouse, lizard, and turtle fragments of FOXP2 could be confirmed by electrophoresis after real-time PCR. Because the C(T) values obtained for human DNA were not affected by contaminating animal DNA at concentrations up to 30 times that of human DNA, we were able to estimate the concentration of human DNA in mixed specimens. This assay provides a reliable and useful method for routine quantification of human-specific DNA in forensic practice.