CD8+T细胞在胃癌组织中的表达及其临床意义

目的 观察胃癌组织中浸润性淋巴细胞CD8+T的表达及临床作用.方法 应用免疫组织化学染色检测102例胃癌患者手术标本中浸润淋巴细胞CD8+T的表达.结果 在胃癌、胃炎及胃息肉组织中均可见浸润淋巴细胞CD8+T的表达,但胃癌组浸润淋巴细胞CD8+T的表达[(29.53±14.71)个/HPF]明显高于慢性胃炎组[(19.54±8.10)个/HPF]和胃息肉组[(14.26±6.83)个/HPF],差异有统计学意义(P＜0.05).CD8+T细胞的表达与胃癌患者的性别、年龄、肿瘤部位、肿瘤大小、淋巴结转移、复发、病理分级和TNM分期无明显相关(P＞0.05);但与组织学类型和浸润深度有关,其中分化型胃癌患者CD8+T细胞的表达[(33.9±18.4)个/HPF]高于低分化型患者的表达[(27.6±12.0)个/HPF],差异有统计学意义(t=2.04,P＜0.05);未侵入肌层组的CD8+T细胞表达[(38.8±13.8)个/HPF]明显高于侵入肌层组[(27.7±14.1)个/HPF],差异有统计学意义(t=2.45,P＜0.05).与CD8+T细胞低表达组比较,CD8+T细胞高表达组胃癌患者中位生存时间延长5个月,但差异无统计学意义(χ2=1.0274,P＞0.05).多因素COX模型分析显示,与浸润淋巴细胞CD8+T低表达组比较,高表达组有降低胃癌死亡风险的趋势(RR=0.82,95%CI=0.41～1.65).结论 浸润性淋巴细胞CD8+T的表达与胃癌的预后有关.

Abstract：

Objective To study the expression and clinical significance of infiltrating lymphocytes CD8 + T in gastric carcinoma tissues.Methods The expression of infiltrating lymphocytes CD8 + T in 102surgically resected specimens of gastric cancer tissues was detected by using immunohistochemistry.Results CD8+ T cells were expressed both in gastric carcinoma,gastritis and gastric polyp tissues,but the number of CD8 + T cells in gastric carcinoma tissues [(29.53 ± 14.71 )/high powered field (HPF)]wassignificantly greater than in gastritis tissues [( 19.54 ±8.10)/HPF]and polyp tissues [( 14.26 ±6.83)/HPF](P ＜0.05).No correlation was found between CD8 + T lymphocyte count and gender,age,tumor location,tumor size,lymph node metastasis,tumor relapse,pathological grade or TNM stage ( P ＞ 0.05 ).However,there was a statistically significant correlation between the number of CD8 + T cells and the histological type,invasive depth.The CD8 + T cell count was obviously higher in the differentiated group [(33.9 ± 18.4)/HPF]than in the undifferentiated group [(27.6 ± 12.0)/HPF]( t = 2.04,P＜0.05 ).And the number of CD8 + T cells was greater in the non-invasive group [(38.8 ± 13.8)/HPF]than in the invasive group [(27.7 ± 14.1 )/HPF]( t = 2.45,P＜0.05 ).The median survival time of gastric cancer patients with high expression of CD8 + T cells was 5 months longer than in those with low expression,but there was no significant difference.Multi-factor COX model analysis demonstrated that the risk of death in gastric cancer patients was lower in the CD8 + T cells high expression group than in the low expression group ( RR = 0.82,95%,CI = 0.41-1.65 ).Conclusion Detection of infiltrating lymphocytes CD8 + T expression in gastric carcinoma tissues will be beneficial to the judgment of the prognosis.     

Expression and clinical significance of FPARγ and β-catenin in breast cancer

Objective To study the expression of peroxisome proliferator-activated receptor γ (PPARγ) and β-catenin in breast cancer, and their correlations with clinicopathological parameters and prognosis. Methods Tissue samples obtained from 70 patients with breast cancer and 20 patients with breast benign mass were immunohistochemically examined for the expression of PPARγ and p-catenin. Results Overexpression rate of PPARγ protein was 34. 3% in breast cancer, significantly lower than that in breast benign mass. Abnormal expression rate of β-catenin in breast cancer was 67. 1%. A significant negative-correlation was found between the expression of PPARγ and β-catenin (r=-0. 398,P<0.05 ). PPARγ expression was inversely associated with histologic grade, tumor size, axillary lymph node metastasis,TNM stage and Ki-67 expression (P<0. 05), while positively correlated with ER status and overall survival rate (P<0. 05). Abnormal β-catenin expression was positively associated with histologic grade, axillary lymph node metastasis and TNM stage (P<0. 05), while inversely correlated with overall survival rate (P<0.05). Conclusion PPARγ and β-catenin are correlated with development of breast carcinoma,suggesting that detection of PPARγ and β-catenin may be of value in evaluating the biological behaviors and the prognosis of breast cancer.     

Expression and clinical significance of FPARγ and β-catenin in breast cancer

Objective To study the expression of peroxisome proliferator-activated receptor γ (PPARγ) and β-catenin in breast cancer, and their correlations with clinicopathological parameters and prognosis. Methods Tissue samples obtained from 70 patients with breast cancer and 20 patients with breast benign mass were immunohistochemically examined for the expression of PPARγ and p-catenin. Results Overexpression rate of PPARγ protein was 34. 3% in breast cancer, significantly lower than that in breast benign mass. Abnormal expression rate of β-catenin in breast cancer was 67. 1%. A significant negative-correlation was found between the expression of PPARγ and β-catenin (r=-0. 398,P<0.05 ). PPARγ expression was inversely associated with histologic grade, tumor size, axillary lymph node metastasis,TNM stage and Ki-67 expression (P<0. 05), while positively correlated with ER status and overall survival rate (P<0. 05). Abnormal β-catenin expression was positively associated with histologic grade, axillary lymph node metastasis and TNM stage (P<0. 05), while inversely correlated with overall survival rate (P<0.05). Conclusion PPARγ and β-catenin are correlated with development of breast carcinoma,suggesting that detection of PPARγ and β-catenin may be of value in evaluating the biological behaviors and the prognosis of breast cancer.     

胃癌神经旁浸润的临床特征与预后分析

Objective To investigate the association between perineural invasion (PNI) and clinicopathological factors and the effect of PNI on overall survival in patients with gastric carcinoma.Methods A total of 178 patients with gastric carcinoma from January 2004 to May 2008 were analyzed retrospectively. Paraffin sections of surgical specimens from all the patients who underwent gastric resection were stained with laminin. PNl-positive was defined as infiltration of carcinoma cells into the perineurium or neural fascicles. The association of PNI with clinicopathologic features and prognosis of gastric carcinoma was studied. Results PNI was positive in 78 of 178 patients (43.8%). The proportions of T stage, lymph node metastasis and TNM stage were significantly higher in PNI-positive group than those in PNl-negative group (all P＜0.01 ). The PNI positive rate was correlated with the depth of gastric mural invasion and clinical stage. The overall survival in PNI-positive group was significantly lower than that in PNI-negative group by univariate analysis (P＜0.01). The mean survival of PNI-positive patients (28.6 months) was significantly shorter than that of PNI-negative patients (44.3 months,P＜0.01), which was also influenced by pN stage, pT stage, and clinical stage(P＜0.0l ). By multivariable Cox proportional hazards model of overall survival, the positivity of PNI appeared to be an independent prognostic factor (hazards ratio=2.257,95% CI: 1.268-4.019, P=0.006).Conclusions PNI is associated with the degree of malignancy in gastric cancer. PNI can be a candidate of new prognostic factor.     

胃癌神经旁浸润的临床特征与预后分析

Objective To investigate the association between perineural invasion (PNI) and clinicopathological factors and the effect of PNI on overall survival in patients with gastric carcinoma.Methods A total of 178 patients with gastric carcinoma from January 2004 to May 2008 were analyzed retrospectively. Paraffin sections of surgical specimens from all the patients who underwent gastric resection were stained with laminin. PNl-positive was defined as infiltration of carcinoma cells into the perineurium or neural fascicles. The association of PNI with clinicopathologic features and prognosis of gastric carcinoma was studied. Results PNI was positive in 78 of 178 patients (43.8%). The proportions of T stage, lymph node metastasis and TNM stage were significantly higher in PNI-positive group than those in PNl-negative group (all P＜0.01 ). The PNI positive rate was correlated with the depth of gastric mural invasion and clinical stage. The overall survival in PNI-positive group was significantly lower than that in PNI-negative group by univariate analysis (P＜0.01). The mean survival of PNI-positive patients (28.6 months) was significantly shorter than that of PNI-negative patients (44.3 months,P＜0.01), which was also influenced by pN stage, pT stage, and clinical stage(P＜0.0l ). By multivariable Cox proportional hazards model of overall survival, the positivity of PNI appeared to be an independent prognostic factor (hazards ratio=2.257,95% CI: 1.268-4.019, P=0.006).Conclusions PNI is associated with the degree of malignancy in gastric cancer. PNI can be a candidate of new prognostic factor.     

胃癌组织中S100P表达的临床意义及其机制探讨

Objective To investigate the impact of the expression of S100P on the prognosis and tumor chemosensitivity in patients with resectable gastric cancer and its mechnisms. Methods The expression of S100P was analyzed in 121 resected primary gastric cancer tissues by using tissue array of immunohistochemistry excised from January 2003 to December 2007. The patients received adjuvant chemotherapy with oxaliplatin. The pEGFP-SlOOP plasmid was constructed and was transfected into BGC823 cell line to establish gastric cancer cell line with over-expression of human S100P, BGC823-S100P. The expression level of S100P was determined by real-time PCR and Western blot assay. The chemosensitivity of BGC823-S10OP cell line to oxaliplatin was detected by 3-( 4, 5-Dimethylthiazol-2-yl )-2, 5-diphenyltetrazolium (MTT) assay. Results The S100P was positively expressed in 64 tumors(52. 9% , 64/ 121). Although there was no significant relation between the expression of S100P and tumor T staging( P = 0. 683), N staging( P = 0. 472), M staging (P = 0. 770 ) and differentiation (P = 0. 553), Wilcoxon test showed that the 5-year cumulative survival rate of patients with positive S100P expression was significantly higher than that of patients with negative expression (20.3% vs. 3.5%, P =0.034). Furthermore, overexpressed of S100P was found in the BGC823 cell line, BGC823-S100P. The mRNA and protein level of S1O0P in pEGFP transfected BGC823-S100P cell lines were significantly higher than those in control group (8.42±1.38 ra. 0.83±0.11 and 3. 52±0.48 vs. 0. 97±0. 19,all P <0. 05). It indicated with MTT assay that the half-inhibitory concentration(IC50) to oxaliplatin decreased in BGC823-S1OOP cells, and was significantly lower than that in vector-only transfected cells[ ( 142±16) mg/L vs. (266± 11 ) mg/L,P = 0. 032]. Conclusions S100P may also be a potentially novel independent prognostic factor in gastric cancer patients following curative resection. And it could improve the cumulative survival of the patients through enhancing the chemosensitivity of tumor cell line to oxaliplatin.     

胃癌组织中S100P表达的临床意义及其机制探讨

Objective To investigate the impact of the expression of S100P on the prognosis and tumor chemosensitivity in patients with resectable gastric cancer and its mechnisms. Methods The expression of S100P was analyzed in 121 resected primary gastric cancer tissues by using tissue array of immunohistochemistry excised from January 2003 to December 2007. The patients received adjuvant chemotherapy with oxaliplatin. The pEGFP-SlOOP plasmid was constructed and was transfected into BGC823 cell line to establish gastric cancer cell line with over-expression of human S100P, BGC823-S100P. The expression level of S100P was determined by real-time PCR and Western blot assay. The chemosensitivity of BGC823-S10OP cell line to oxaliplatin was detected by 3-( 4, 5-Dimethylthiazol-2-yl )-2, 5-diphenyltetrazolium (MTT) assay. Results The S100P was positively expressed in 64 tumors(52. 9% , 64/ 121). Although there was no significant relation between the expression of S100P and tumor T staging( P = 0. 683), N staging( P = 0. 472), M staging (P = 0. 770 ) and differentiation (P = 0. 553), Wilcoxon test showed that the 5-year cumulative survival rate of patients with positive S100P expression was significantly higher than that of patients with negative expression (20.3% vs. 3.5%, P =0.034). Furthermore, overexpressed of S100P was found in the BGC823 cell line, BGC823-S100P. The mRNA and protein level of S1O0P in pEGFP transfected BGC823-S100P cell lines were significantly higher than those in control group (8.42±1.38 ra. 0.83±0.11 and 3. 52±0.48 vs. 0. 97±0. 19,all P <0. 05). It indicated with MTT assay that the half-inhibitory concentration(IC50) to oxaliplatin decreased in BGC823-S1OOP cells, and was significantly lower than that in vector-only transfected cells[ ( 142±16) mg/L vs. (266± 11 ) mg/L,P = 0. 032]. Conclusions S100P may also be a potentially novel independent prognostic factor in gastric cancer patients following curative resection. And it could improve the cumulative survival of the patients through enhancing the chemosensitivity of tumor cell line to oxaliplatin.     

DNA甲基转移酶在肝癌中的表达及其临床意义

Objective To investigate the expression of DNA methyltransferases (DNMTs) in liver cancer and its clinical significance. Methods The specimens of liver cancer tissues, adjacent tissues, cirrhotic tissues and chronic hepatitis tissues were collected from 50 patients who received radical resection at the First Affiliated Hospital of Sun Yat-Sen University from July 2007 to April 2008. The mRNA and protein expressions of DNMT1,DNMT3a and DNMT3b in liver cancer tissues, adjacent tissues, cirrhotic tissues and chronic hepatitis tissues were detected by real-time quantitative PCR and immunohistochemical staining. The mRNA expression of DNMTs in the liver cancer tissues was compared with those in the adjacent tissues, cirrhotic tissues and chronic hepatitis tissues by using t test and Mann-Whitney U test. The correlation between the protein expression of DNMTs in the liver cancer tissue and the clinicopathological features was analyzed by chi-square test or Fisher exact test, and the tumor-free survival time was analyzed by using Kaplan-Meier method and the difference in tumor-free survival rate between different patients was analyzed by Log-rank test. Results The mRNA expressions of DNMT1, DNMT3a and DNMT3b in the liver cancer tissue were 2.57, 2.29 and 4.86 times higher than those in the adjacent tissues (t = 3.94, 2. 72, 4. 06, P ＜ 0.05 ). The mRNA expressions of DNMT1, DNMT3a and DNMT3b were 2.38,2.14 and 4.66 times higher than those in the cirrhotic tissues, and 6.12, 4.58 and 12.99 times higher than those in the chronic hepatitis tissues. The mRNA expressions of DNMT1, DNMT3a and DNMT3b in the liver cancer tissue were significantly higher than those in the cirrhotic tissues and chronic hepatitis tissues ( U = 587.5,730. 0,562.5; 65.5, 64.5, 71.0, P ＜ 0.05). The protein expression of DNMT1 was correlated with the size, number,TNM stages and vascular invasion of tumors ( x2 = 4.08, 5.95, 4.08, P ＜ 0.05 ). The protein expression of DNMT3a was correlated with the size, number and TNM stages of tumors (x2 = 4.08, 5.95, 4.08, P ＜ 0.05 ).The mean tumor recurrence time of patients with low expressions of DNMT1 and DNMT3a were 9.4 and 8.7 months, which were significantly longer than 5.0 and 3.2 months of those with high expressions of DNMT1 and DNMT3a (x2 =3.89, 9.91, P＜0.05). Conclusions DNMTs play an important role in hepatocarcinogenesis.High expressions of DNMT1 and DNMT3a are correlated with the postoperative recurrence of liver cancer, which are valuable prognostic factors for liver cancer.     

胰腺癌中磷酸酶和张力蛋白同源物基因及B7-H1蛋白的表达及其临床意义

Objective To investigate the probable correlation between the expressions of phosphatase and tensin homologue deleted on chromosometen (PTEN) and B7-H1 protein in pancreatic carcinoma and the biological behavior characteristics of tumors. Methods Forty-three patients were recruited who had undergone surgical resection for pancreatic carcinoma between 2002 and 2009. The PTEN and B7-H1 protein expressions in the tissue specimens of these 43 patients and 5 non-pancreatic carcinoma people' s pancreatic tissue specimens were evaluated by immunohistochemistry ELPS technique, and the clinical and pathological features of these specimens and the follow-up information were analyzed. Results PTEN expressions were significantly lower in pancreatic carcinoma tissues than in non-pancreatic carcinoma people' s pancreatic tissues but B7-H1 expressions were significantly higher ( P ＜ 0. 01 ). The expression of PTEN was negatively correlated to that of B7-H1 (r = -0.414 ,P ＜0. 01). PTEN and B7-H1 expressions correlated with the pathological grade and tumor-node-metastasis ( TNM ) stage, peripancreatic invasion, regional lymph node involvement,respectively (P＜0. 05). B7-H1 expressions also significantly correlated with the ages (P＜0. 01). Furthermore, PTEN and B7-H1 expressions showed significant prognostic effects (P＜0.01) and there are correlations existed between combined PTEN/B7-H1 expression and prognostic effects (P ＜0. 05). Conclusion The expression of PTEN and B7-H1 may be significantly correlated to the carcinogenesis,development and prognosis of pancreatic carcinoma.     

肿瘤转移抑制蛋白在肝癌中的表达及其意义

目的 探讨肿瘤转移抑制蛋白1(metastasis suppressor 1,MTSS1)在肝癌组织,肝硬化组织,正常肝组织中的表达及其意义.方法采用免疫组织化学检测MTSS1在肝癌组织,肝硬化组织,正常肝组织中的表达,并用单因素分析表达与临床病理因素的关系.用Spearman等级相关分析MTSSl表达水平与肝癌患者TNM分期的关系.对肝癌患者进行5年生存随访,采用Kaplan-Meier生存曲线分析.结果 肝癌组织MTSS1表达水平与正常肝组织比较,差异有统计学意义(U=168.000,P＜0.05);肝癌组织比肝硬化组织高,二者比较差异有统计学意义(U=106.000,P＜0.05);MTSS1表达水平与肝癌患者的TNM分期、有无血管侵袭和肿瘤包膜有关(分别U=259.000,258.500,202.000,均P＜0.05),与肝癌患者的年龄、性别、肿瘤大小、AFP水平、乙肝表面抗原无关(P＞0.05).MTSS1表达水平与临床TNM分级间呈负相关,即临床TNM分级越早期所对应的MTSS1表达水平越高(rs=-0.383,P＜0.05).MTSS1阳性表达患者5年生存率明显低于MTSS1阴性及弱阳性表达患者,差异有统计学意义(分别34.1%,52.3%,x2=6.386,P＜0.05).结论 MTSS1高表达可能在早期肝癌进展中发挥重要作用,预示预后不良.

Abstract：

Objective To explore the expression and significance of MTSS1 ( metastasis suppressor 1) in hepatocellular carcinoma.Methods MTSS1 expression was detected by immunohistochemistry in hepatocellular carcinoma, liver cirrhosis and normal liver tissues.Single-factor analysis was used to study the relationship with clinicopathological factor.Correlations between MTSS1 expression and TNM stage were analyzed with Spearman rank correlation analysis.Postoperative 5-year survival was evaluated using Kaplan-Meier survival curve analysis.Results The expression of MTSS1 in hepatocellular carcinoma was higher than normal liver tissue ( U = 168.000, P ＜ 0.05), and liver cirrhotic tissue ( U = 106.000, P ＜ 0.05); MTSS1 expression was correlated with TNM stage of hepatocellular carcinoma patients, lymph vascular invasion and tumor capsule ( separately U = 259.000, 258.500, 202.000, all P ＜ 0.05).MTSS1 expression in hepatocellular carcinoma was not correlated with patients age, gender, tumor size, AFP level, and hepatitis B surface antigen.MTSS1 expression and TNM stage of liver cancer patients was negatively correlated ( rs = - 0.383 , P ＜ 0.05 ).Postoperative 5-year survival of hepatocellular carcinoma patients with MTSS1 positive expression was significantly poorer than patients with negative and weakly positive expression (respectively 34.1% and 52.3% , x2 =6.386, P ＜ 0.05).Conclusions MTSS1 high expression may play an important role in the early hepatocellular carcinoma progression, indicating a poor prognosis.     

高表达CXCL12、IGF1胃癌组织临床病理特征及预后分析

目的探讨高表达CXCL12、IGF1胃癌组织临床病理特征及预后,为临床胃癌的诊断和治疗提供依据。 方法回顾性分析2012年3月至2015年3月81例进行手术切除的81例胃癌患者临床资料,采用免疫组织化学(IHC)方法检测患者癌组织与癌旁组织(距离癌组织1 cm)及正常组织中CXCL12与IGF1表达水平。数据以SPSS20.0统计分析,CXCL12与IGF1在不同胃组织中表达水平以例数(%)表示,采用χ²检验;使用Kaplan－Meier法绘制生存曲线,使用Log\\rank法比较生存曲线之间的差异,以P<0.05为差异有统计学意义。 结果胃癌组织中CXCL12(53.1%)、IGF1(49.4%)表达水平明显高于癌旁组织(40.7%,39.5%)与正常组织(7.4%,8.6%), P<0.05;高表达CXCL12(中位生存时间为13个月)、IGF1(中位生存时间为17个月)胃癌患者生存时间明显较低表达(中位生存时间分别为64、65个月)患者,差异有统计学意义(P<0.05)。 结论CXCL12、IGF1在胃癌中表达水平显著高于正常组织和癌旁组织,可能作为胃癌诊断的依据,高表达CXCL12、IGF1胃癌患者恶性程度较高、较易出现转移,患者预后较差。     

胸苷磷酸化酶在消化道恶性肿瘤中的表达及预后价值

目的观察胸苷磷酸化酶（TP）在消化道恶性肿瘤的表达以及与肿瘤血管生成的关系，探讨TP对肿瘤预后的价值。方法采用免疫组织化学方法检测181例消化道恶性肿瘤组织和对应的77例邻近正常组织的TP表达和微血管密度（MVD），比较不同肿瘤间及肿瘤与正常组织TP表达差异。分析肿瘤组织TP表达与MVD值的关系。以Kaplan—Meier生存曲线分析胃癌、大肠癌TP表达对预后的意义。结果TP在胃癌、大肠癌、肝癌及胰腺癌的阳性表达率依次为62．2％、63．5％、55．0％和68．2％，且肿瘤组织的表达率均显著高于正常组织（P〈0．05）；TP阳性组的MVD值均显著高于阴性组（P〈0．05）；Kaplan—Meier生存曲线提示：胃癌、大肠癌中TP阳性表达者的预后明显较阴性表达者差（P〈0．05）。结论TP在消化道恶性肿瘤中呈高表达并对肿瘤血管生成有促进作用，对胃癌、大肠癌患者的预后评估有一定价值。     

胃癌组织中Omi/HtrA2的表达及与预后的关系

目的 探讨丝氨酸蛋白酶Omi/HtrA2在胃癌组织中的表达及其与胃癌临床病理特征及预后的关系.方法 采用免疫组化法检测68例胃癌组织、15例癌旁组织及15例正常胃黏膜组织中Omi/HtrA2的表达,并分析其表达与胃癌临床病理特征及预后的关系.结果 Omi/HtrA2在胃癌组织中的阳性表达率为73.5%（50/68）,高于癌旁组织（13.3%,2/15）和正常胃黏膜（6.7%,1/15）,差异有统计学意义（P＜0.05）.胃癌组织中Omi/HtrA2的表达与患者的性别、年龄、肿瘤大小及浸润深度无关（P＞0.05） 与肿瘤的分化程度、淋巴结转移和临床分期有关（P＜0.05）.本组胃癌患者5年总体生存率为63.3%,其中Omi/HtrA2表达阳性组和阴性组分别为72.0%和61.1%,两组比较,差异并无统计学意义（P＞0.05）.结论 Omi/HtrA2在胃癌组织中高表达,其表达与胃癌分化程度、淋巴结转移及TNM分期有关,但并不影响胃癌患者的预后.     

保罗样激酶1表达对胃癌的生物学行为及预后判定的意义

目的探讨保罗样激酶1(plk1)在胃癌组织中的表达情况以及与胃癌临床病理指标、患者预后的关系。方法采用免疫组织化学回顾检测plK1在89例切除胃癌组织中的表达,分析其与胃癌临床病理学指标的关系;并分析plK1表达与患者生存的关系。结果plk1在胃癌组织中的阳性率为42.7%(38/89),明显高于邻近非癌组织的13.5%(12/89)(P<0.01)。plk1基因表达与胃癌的分化程度、浸润深度、TNM分期密切相关(P<0.05)。plk1表达阳性患者的预后明显较阴性患者差(P<0.05)。结论plk1基因可能在胃癌发生发展中起促进作用,其过表达程度可作为胃癌的某些生物学行为及判定预后的新的参考指标。     

miR-214和miR-181c在胃癌组织中的表达及预后价值

目的探讨微小RNA-214（miR-214）和miR-181c在胃癌组织中的表达水平及对预后的影响。 方法选取2014年1月至2015年1月于川北医学院附属医院收治的68例胃癌患者为研究对象,均接受手术治疗,出院后随访1~60个月。利用实时荧光定量PCR技术检测患者癌组织和癌旁组织miR-214、miR-181c相对表达量;利用Kaplan-Meier曲线进行生存分析;Cox多因素回归分析影响胃癌患者预后的独立危险因素。 结果胃癌组织中miR-214、miR-181c表达水平均明显低于癌旁组织,差异有统计学意义（P<0.05）。根据miR-214、miR-181c表达均值将患者分为高表达组和低表达组,miR-214、miR-181c表达水平与年龄、性别、淋巴结是否转移无关,与TNM分期、肿瘤分化程度有关（P<0.05）。患者总生存率为44.12%,miR-214低表达组和高表达组术后5年累积生存率分别为35.71%、57.69%,两组间比较差异有统计学意义（P=0.035）;miR-181c低表达组和高表达组术后5年累积生存率分别为35.55%、60.87%,差异有统计学意义（P=0.024）。Cox多因素回归分析结果显示,TNM分期高（HR=1.569,95% CI：1.029~2.391,P=0.036）、miR-214低表达（HR=1.643,95% CI：1.294~2.087,P<0.001）及miR-181c低表达（HR=1.327,95% CI：1.045~1.685,P=0.021）是影响胃癌患者预后的独立危险因素。 结论miR-214、miR-181c在胃癌组织中表达显著下调,与胃癌患者临床病理参数及不良预后有关,参与胃癌的发生发展过程。     

胃癌组织中AEG-1、HER2的表达及临床意义

目的探讨胃癌组织中AEG-1、HER-2的表达及与胃癌临床病理特征的相关性。 方法收集2013年1月至2018年1月96例胃癌患者组织切片,采用免疫组织化学法检测AEG-1、HER-2表达情况。在以上胃癌患者中随机选取20例,取距肿瘤5 cm以上的癌旁胃组织作为对照组。使用SPSS16.0统计软件进行分析,依据不同分析目的分别采用χ²检验、线性趋势检验、Spearman等级相关分析及Kaplan-Meier生存分析,P<0.05为差异有统计学意义。 结果胃癌组织中AEG-1阳性表达率(76.0%)明显高于癌旁胃组织(0%),其阳性表达率与分化程度、TNM分期、淋巴结转移有关(P<0.05)。胃癌组织中HER2阳性表达率(41.7%)明显高于癌旁胃组织(0%),其阳性表达率与分化程度、浸润深度、TNM分期、淋巴结转移有关(P<0.05)。胃癌组织中AEG-1、HER2阳性表达呈正相关(r＝0.276,P＝0.007)。Kaplan-Meier生存分析提示胃癌中AEG-1及HER2双阴性表达患者的预后(平均生存期：31.1个月)优于AEG-1及HER2双阳性表达患者(平均生存期：48.3个月)。 结论胃癌组织中可能存在AEG-1、HER2信号通路。检测胃癌组织中AEG-1、HER2的表达,有利于判断胃癌的发生、发展、浸润、转移及预后。     

表皮生长因子受体和蛋白激酶B在胃癌组织中的表达及临床意义

目的探讨胃癌组织表皮生长因子受体（EGFR）和蛋白激酶B（AKT）在胃癌组织中的表达与胃癌临床病理特征及预后的关系。方法采用免疫组织化学方法检测84例胃癌组织和15例非癌胃组织中EGFR和AKT的表达情况,并分析其表达与胃癌患者临床病理特征及预后的关系。结果EGFR和AKT在胃癌组织的阳性表达率分别为55．9％（47／84）和64．3％（54／84）,明显高于非癌胃组织的20．0％（3／15）和6．7％（1／15）,差异均有统计学意义（P〈0．05,P〈0．01）。EGFR表达与胃癌分期有关,AKT表达则与胃癌淋巴结转移有关（均P〈0．01）。EGFR阴性表达和阳性表达患者5年总体生存率分别为49％和22％（P〈0．05）;AKT阴性表达和阳性表达患者5年总体生存率分别为45．7和30．2％（P〈0．05）。结论检测胃癌组织中的EGFR和AKT的表达,有助于判断胃癌恶性程度及评估预后。     

醛酮还原酶1-B10在胃癌组织中的表达及其临床意义

目的探讨醛酮还原酶1-BIO在胃癌组织中的表达及其与临床病理特征和预后的关系。方法通过荧光定量PCR法检测36例胃癌组织及其配对癌旁组织中AKRlBl0mRNA的表达量,采用免疫组织化学法检测100例原发胃癌组织及70例非肿瘤胃黏膜组织中AKRlB10蛋白表达情况。结果PCR结果显示,91．7％（33／36）癌旁非肿瘤胃黏膜组织中AKRlB10mRNA的表达高于其配对胃癌组织[8．3％（3／36）,P=0．000]。100例胃癌组织中33例（33．0％）AKRIBl0蛋白阳性表达,70例非肿瘤胃黏膜上皮中65例（92．9％）AKRlB10的阳性表达。两者比较,差异有统计学意义（Jp=0．000）。胃癌组织中AKRIBIO的表达与患者性别、年龄、肿瘤部位及分化程度无关（p〉0．05）,而与肿瘤大小（P=O．000）、浸润深度（P=0．004）、淋巴结转移（P=0．028）、远处转移（P=0．031）和临床TNM分期（P=0．000）有关。AKRlB10阳性表达患者的5年生存率显著高于阴性表达者（60．6％比32．8％,P〈0．01）。结论胃癌组织中AKRlB10的表达下调与胃癌进展有关,提示预后不良。     

Bcl-w基因在胃癌中的表达和临床病理因素的关系

目的 探讨B细胞淋巴因子-w(Bcl-w)在胃癌中的表达与预后的关系.方法 通过免疫组化SP法,检测100例胃癌组织中Bcl-w和MMP-2(基质金属蛋白-2)的表达.结果 (1)100例胃癌中Bcl-w和MMP-2表达的阳性率分别为69.0%、77.0%;(2)胃癌组织中Bcl-w和MMP-2的表达与肿瘤的大小、浸润...     

干细胞标记物Lgr5在胃癌组织中的表达及临床意义

目的分析干细胞标记物Lgr5（富含亮氨酸重复单位的G蛋白偶联受体5）蛋白在人胃癌组织中的异常表达,并探讨在胃癌肿瘤的发生发展等过程中发挥的作用。 方法选取2012年1月至2015年6月接受胃癌手术治疗的70例患者,采用免疫组化法分别检测70例胃正常组织、癌旁组织、胃腺癌标本中Lgr5的蛋白表达,并统计学分析其与临床病理指标、患者生存预后的关系。 结果70例胃癌组织中的Lgr5蛋白阳性表达率（50/70,71.4%）明显高于癌旁组织（35/70,50.0%）、正常组织（21/70,30.0%）,差异有统计学意义（χ²=6.738、24.034,P=0.009、0.000）;Lgr5蛋白的表达与胃癌患者的性别、年龄及分化程度无明显相关性,与淋巴结节转移、TNM分期密切相关（均P<0.05）。随访胃癌患者66例,其中Lgr5阳性组47例,Lgr5阴性组19例,生存分析两组术后3年生存率差异有统计学意义（χ²=3.929,P=0.047）。 结论Lgr5蛋白在胃癌发生过程中可能发挥一定作用,它与胃癌的侵袭、转移和预后明显相关,Lgr5的表达检测可作为判断胃癌患者预后的一种手段。