荷负电气溶胶促进烧伤患者头部取皮区创面愈合效果观察

目的探讨荷负电气溶胶促进烧伤患者头部取皮区创面愈合的效果。方法将120例患者随机分为气溶胶组（61例）和对照组（59例），两组均采用电动取皮机取头皮，厚度0．25mm。取皮后对照组采用常规处理；气溶胶组采用气溶胶对头部取皮区创面进行治疗护理，每次2h，每天2次，直至创面愈合。结果两组创面疼痛消失、创面渗出物消失情况及愈合时间比较，差异有显著性意义（P〈0.05、P〈0.01）。结论荷负电气溶胶能有效促进烧伤患者头部取皮区创面愈合。     

荷负电气溶胶治疗Ⅱ度烧伤创面的临床效果及病理学观察

目的观察荷负电气溶胶(下称气溶胶)治疗Ⅱ度烧伤创面的效果。方法选择单纯浅Ⅱ、深Ⅱ度烧伤患者,随机分为:(1)气溶胶组:浅Ⅱ度180例、深Ⅱ度100例,伤后6h~2d开始用气溶胶治疗创面,l~2次/d,1.5h/次。(2)对照组:浅Ⅱ、深Ⅱ度患者各30例,常规治疗。(3)自身对照组:浅Ⅱ、深Ⅱ度患者各10例,同上用气溶胶治疗,但同一患者部分创面覆盖无菌金属片屏蔽气溶胶(屏蔽组),部分创面不屏蔽(非屏蔽组)。观察气溶胶治疗过程中患者创面的大体变化,治疗前后进行创面细菌培养,并监测其肝、肾功能及血生化指标有无改变。记录各组患者创面愈合时间。另制作深Ⅱ度烫伤大鼠模型,同前分为气溶胶组和对照组并治疗。取两组大鼠治疗前及治疗后1、2、3周的创面组织标本,作病理学观察。结果气溶胶治疗后患者创面渗出少,治疗前后均无细菌生长。总体来讲,气溶胶治疗前后患者肝、肾功能及血生化指标无明显改变。气溶胶组患者浅Ⅱ度创面伤后(6.3±1.6)d愈合,深Ⅱ度创面(15.1±3.1)d愈合,明显短于对照组相同深度创面[(11.3±1.4)、(21.2±1.4)d,P<0.01]。自身对照组中,相同烧伤深度的非屏蔽组与屏蔽组比较,创面愈合时间也明显缩短(P<0.01)。病理学检查显示,气溶胶组大鼠治疗后第3周皮肤结构已基本恢复正常,而对照组此时恢复较差。结论气溶胶能有效促进Ⅱ度烧伤创面的愈合且使用安全。     

荷负电气溶胶治疗大鼠烧伤创面后表皮干细胞的变化

目的探讨荷负电气溶胶对烧伤创面表皮干细胞影响以及其促进创面愈合的机制。方法将清洁级健康Wistar大鼠48只随机分为A、B两组，每个动物背部两侧分别制成实验创面或对照创面，实验创面以荷负电气溶胶照射，A组动物每日照射2次，B组每日照射1次；利用干细胞表达β1整合素的特性，加入整合素β1抗体，通过免疫组织化学染色检测创面表皮干细胞含量的变化，比较各时相点两组创面间的差异。结果A、B两组各时相点实验创面的表皮干细胞数目是同组对照创面的1．5到2倍；而A组实验创面的表皮干细胞数又较B组实验创面增多。结论经用荷负电气溶胶照射大鼠烧伤创面能促进表皮干细胞的增殖。     

重视烧伤后补锌,促进创面愈合

烧伤创面始终是烧伤治疗的焦点，创面愈合一直是烧伤治疗的目标。影响创面愈合的诸多因素己被普遍关注，但有关微量元素锌与创面愈合的关系论述欠丰。近年来大量研究证实，严重烧伤后普遍缺锌，除对整体产生不良影响外，还使创面愈合延迟。补锌确有改善锌营养状态，促进创面修复之功效。 一、锌在创面愈合中的作用 成人体内约含锌１．４－２．３ｇ，在各微量元素含量中仅次于铁，居第二位［１］。其中皮肤锌即占体内锌总量的２０％，表皮含锌量明显多于真皮，表皮为７０．５ ｇ／ｋｇ干重，真皮为 １２．６ ｇ／ｋｇ干重，表明锌是维持皮肤…     

荷负电气溶胶治疗对大鼠烫伤创面白细胞介素-8、10表达的影响及意义

目的 观察荷负电气溶胶治疗对大鼠烫伤创面愈合过程中白细胞介素(IL)-8、IL-10表达的影响,探讨荷负电气溶胶治疗促进创面愈合的作用机制.方法 制作SD大鼠深Ⅱ度烫伤模型,采用分组对照方法,将40只鼠随机分为治疗组(n1=20)和对照组(n2=20).治疗组应用荷负电气溶胶治疗,每次1.5 h,每天2次,直至创面愈合;对照组不作荷负电气溶胶治疗.伤后第1～11天分别取创面标本制作切片,采用免疫组织化学和图像分析方法,检测创面愈合过程中IL-8和IL-10表达水平.结果 创面平均愈合时间治疗组为(7.00±1.15)d,对照组为(9.00±1.34)d,治疗组创面愈合时间明显提前(P＜0.01).免疫组织化学显示,两组IL-8均在伤后第1天开始表达.主要位于多核粒细胞和单核细胞;第3天表达明显增多达高峰,并见大量成纤维细胞表达,治疗组的峰值明显低于对照组,差异有统计学意义(P＜0.01),第5～11天表达水平迅速下降.两组IL-10伤后第1天在淋巴细胞和单核细胞均有表达;第3天开始有角质细胞表达并达高峰,第5～11天表达水平缓慢下降,但治疗组要明显高于对照组,第3～11天差异有统计学意义(P＜0.01).结论 荷负电气溶胶治疗能有效抑制创面IL-8的表达及促进IL-10的表达,缩短炎症进程,从而加速创面愈合.

Abstract：

Objective To discuss the influence of aerosol bioelectricity on the expression of interleukin (IL) -8 and IL-10 in wound healing of burned rats. Methods The deep Ⅱ degree scalding models were established in Sprague Dawley (SD) rats. Rats were randomly divided into experimental group (n1 =20) and control group (n2 =20). The rats in experimental group were treated with aerosol bioelectricity.Samples were collected at the first to eleventh day post-scalding. Immunohistochemistry and image analysis methods were conducted to examine the expression of IL-8 and IL-10 in both experimental and control groups. Results The average wound healing time in experimental group was 7. 00 ± 1. 15 days, and that in control group was 9. 00 ± 1. 34 days. IL-8 and IL-10 were observed mainly in polylmorphonuclear and mononuclear cells in both experimental and control groups on the 1 st day. On the third day, fibroblasts abounded, IL-8 expression was increased evidently and reached a peak. The peak value (6. 73 ± 1. 36) in experimental group was lower significantly than that in control group ( 2. 85 ± 0. 72, P ＜ 0. 01). From the 5th to 11th day, IL-8 expression was declined rapidly. IL-10 was expressed in keratode cells and had the peak value in experimental group (1. 24 ±0. 15) and control group (5. 69 ± 1. 32) on the 3rd day. IL-10 expression was declined gradually from the 5th to 11th days. The expression level of IL-10 in experimental group was significantly higher than in control group from the 3rd day to 11th days post-scalding (P＜0. 01). On the 3rd day, both IL-8 and IL-10 in experimental and control groups were expressed abundantly , and there was negative relationship between them (r = - 0. 862, P ＜ 0. 01). Conclusion Aerosol bioelectricity can indicate active cells proliferation through down-regulating the expression of IL-8 and up-regulating the expression of IL-10, accelerating burned wound healing.     

荷负电气溶胶治疗对大鼠烫伤创面白细胞介素-8、10表达的影响及意义

Objective To discuss the influence of aerosol bioelectricity on the expression of interleukin (IL) -8 and IL-10 in wound healing of burned rats. Methods The deep Ⅱ degree scalding models were established in Sprague Dawley (SD) rats. Rats were randomly divided into experimental group (n1 =20) and control group (n2 =20). The rats in experimental group were treated with aerosol bioelectricity.Samples were collected at the first to eleventh day post-scalding. Immunohistochemistry and image analysis methods were conducted to examine the expression of IL-8 and IL-10 in both experimental and control groups. Results The average wound healing time in experimental group was 7. 00 ± 1. 15 days, and that in control group was 9. 00 ± 1. 34 days. IL-8 and IL-10 were observed mainly in polylmorphonuclear and mononuclear cells in both experimental and control groups on the 1 st day. On the third day, fibroblasts abounded, IL-8 expression was increased evidently and reached a peak. The peak value (6. 73 ± 1. 36) in experimental group was lower significantly than that in control group ( 2. 85 ± 0. 72, P ＜ 0. 01). From the 5th to 11th day, IL-8 expression was declined rapidly. IL-10 was expressed in keratode cells and had the peak value in experimental group (1. 24 ±0. 15) and control group (5. 69 ± 1. 32) on the 3rd day. IL-10 expression was declined gradually from the 5th to 11th days. The expression level of IL-10 in experimental group was significantly higher than in control group from the 3rd day to 11th days post-scalding (P＜0. 01). On the 3rd day, both IL-8 and IL-10 in experimental and control groups were expressed abundantly , and there was negative relationship between them (r = - 0. 862, P ＜ 0. 01). Conclusion Aerosol bioelectricity can indicate active cells proliferation through down-regulating the expression of IL-8 and up-regulating the expression of IL-10, accelerating burned wound healing.     

荷负电气溶胶治疗对大鼠烫伤创面白细胞介素-8、10表达的影响及意义

Objective To discuss the influence of aerosol bioelectricity on the expression of interleukin (IL) -8 and IL-10 in wound healing of burned rats. Methods The deep Ⅱ degree scalding models were established in Sprague Dawley (SD) rats. Rats were randomly divided into experimental group (n1 =20) and control group (n2 =20). The rats in experimental group were treated with aerosol bioelectricity.Samples were collected at the first to eleventh day post-scalding. Immunohistochemistry and image analysis methods were conducted to examine the expression of IL-8 and IL-10 in both experimental and control groups. Results The average wound healing time in experimental group was 7. 00 ± 1. 15 days, and that in control group was 9. 00 ± 1. 34 days. IL-8 and IL-10 were observed mainly in polylmorphonuclear and mononuclear cells in both experimental and control groups on the 1 st day. On the third day, fibroblasts abounded, IL-8 expression was increased evidently and reached a peak. The peak value (6. 73 ± 1. 36) in experimental group was lower significantly than that in control group ( 2. 85 ± 0. 72, P ＜ 0. 01). From the 5th to 11th day, IL-8 expression was declined rapidly. IL-10 was expressed in keratode cells and had the peak value in experimental group (1. 24 ±0. 15) and control group (5. 69 ± 1. 32) on the 3rd day. IL-10 expression was declined gradually from the 5th to 11th days. The expression level of IL-10 in experimental group was significantly higher than in control group from the 3rd day to 11th days post-scalding (P＜0. 01). On the 3rd day, both IL-8 and IL-10 in experimental and control groups were expressed abundantly , and there was negative relationship between them (r = - 0. 862, P ＜ 0. 01). Conclusion Aerosol bioelectricity can indicate active cells proliferation through down-regulating the expression of IL-8 and up-regulating the expression of IL-10, accelerating burned wound healing.     

不同取皮方法、创面不同处理对烧伤手术供皮区愈合影响的研究

目的回顾性研究了我院近5年来烧伤病人供皮区取皮方法的改进和供皮区创面用药的变化与供皮区创面愈合时间的变化。方法总结了我院自2002年以来收治的烧伤住院手术病人1523例,按辊轴手取刀与电动取皮刀,比较了取皮创面基底的均匀程度及创面愈合后边缘是否整齐;创面应用重组人表皮生长因子(rhEGF)与创面单纯应用油纱布在创面愈合时间的对比。结果电动取皮刀在供皮区创面取皮时所取的供皮厚度明显均匀,创面愈合后边缘整齐;应用重组人表皮生长因子(rhEGF)的创面愈合时间比单纯应用油纱布的创面愈合时间明显缩短。结论应用电动取皮刀取皮,可以使皮片厚度均匀,创面愈合后边缘整齐;创面应用rhEGF,能明显加快创面愈合,有利于烧伤创面的愈合。     

碱性成纤维细胞生长因子凝胶治疗烧伤患者供皮区创面的疗效观察

目的 探讨bFGF凝胶对烧伤患者供皮区创面的疗效. 方法 2012年9-12月,选取笔者单位收治的30例烧伤患者共42个供皮区创面,按皮片厚度划分为中厚皮片供皮区8个、刃厚皮片供皮区34个,按部位划分为头部供皮区28个、大腿外侧供皮区8个、背部供皮区6个.供皮区面积为100 ～200(160±30)cm2.应用bFGF凝胶夹在2层凡士林油纱中间共2次用药,具体原则为每平方厘米涂抹150 U的bFGF凝胶(厚约2 mm),术后7、10、14 d换药时以视觉模拟评分表(VAS)测定供皮区创面疼痛程度并评分,小于4分为无痛或轻度疼痛.拍照观察创面愈合情况,采用Photo-shop 8.0软件计算创面愈合率. 结果 所有患者供皮区换药时VAS评分均小于4分.34个刃厚皮片供皮区创面均于术后7d愈合.6个中厚皮片供皮区创面于术后10 d愈合;2个大腿部中厚皮片供皮区术后10 d愈合率分别为70％和80％,术后14 d完全愈合. 结论 bFGF凝胶治疗烧伤患者供皮区术后创面具有较好效果.     

软组织扩张术对烧伤整形患者创面愈合情况的影响

目的 探讨烧伤整形患者采用软组织扩张术对创面愈合情况的影响。方法 选取我院2019年 7月-2022年6月收治的30例烧伤整形患者为研究对象,根据随机数字表法分成研究组与对照组,每组15例。 对照组采用复合皮瓣移植术,研究组采用软组织扩张术,比较两组临床疗效、创面愈合情况、疼痛程度、 生命质量、瘢痕修复效果及并发症发生情况。结果 研究组治疗总有效率为93.33%,高于对照组的60.00% （P＜0.05）;研究组创面愈合时间、皮肤血运恢复时间短于对照组（P＜0.05）;两组术后24 h VAS评分均 低于术前,且研究组低于对照组（P＜0.05）;两组术后3个月BSHS-B评分均高于术前,且研究组高于对 照组（P＜0.05）;两组术后3个月外观、质地、弹性评分均高于术前,且研究组高于对照组（P＜0.05）;研 究组并发症总发生率为6.67%,低于对照组的46.67%（P＜0.05）。结论 对烧伤整形患者行软组织扩张术可提 高临床疗效,改善创面愈合情况,缓解疼痛程度,提高生活质量及瘢痕修复效果,减少并发症发生几率。     

水胶体敷料在大面积烧伤患者中的应用效果及促进创面愈合机制探讨

目的:分析水胶体敷料在大面积烧伤患者中的应用效果及其促进创面愈合机制。方法:选择笔者医院2018年1月-2019年6月收治的200例大面积烧伤患者,以随机数表法分为采取纱布加压包扎的对照组以及采取水胶体敷料包扎的观察组,各100例,两组均接受Meek植皮术治疗,对两组创面愈合时间、创面细菌定量、炎性细胞因子(C反应蛋白、白介素6、转化生长因子-β1)、血管新生指标(血管内皮生长因子、碱性成纤维细胞生长因子)进行比较。结果:观察组创面愈合时间(19.85±1.15)d,短于对照组(25.50±1.50)d,创面细菌定量(420.25±15.20)cfu/g小于对照组(877.50±15.35)cfu/g,差异有统计学意义(t=29.893、211.667,P均=0.000)。治疗后观察组C反应蛋白(9.25±1.05)mg/L、白介素6(71.10±12.10)ng/L、转化生长因子-β1(9.33±1.17)μg/L,低于对照组(12.11±1.08)mg/L、(84.88±12.82)ng/L、(12.14±1.12)μg/L,差异有统计学意义(t=18.987、77.245、17.349,P均=0.000)。观察组血管内皮生长因子(140.29±1.61)ng/L、碱性成纤维细胞生长因子(88.92±1.18)ng/L,高于对照组(124.38±1.52)ng/L、(79.86±1.24)ng/L,差异有统计学意义(t=71.856、52.929,P均=0.000)。结论:在大面积烧伤患者治疗中水胶体敷料能够通过抑制炎症反应、促进血管新生来促进创面愈合,值得在今后临床治疗工作中推广使用。     

综合护理在深度烧伤患者实施湿润烧伤膏创面修复中的应用

目的 探讨在使用湿润烧伤膏进行创面修复的深度烧伤患者中采用综合护理的效果。方法 选取 2020年12月-2023年12月本院收治的80例深度烧伤患者为研究对象,采用随机数字表法分为对照组 （n =40）和研究组（n =40）。两组均接受湿润烧伤膏创面修复治疗,对照组给予常规护理,研究组给予 综合护理,比较两组疼痛程度、负性情绪、瘢痕情况、生活质量及并发症发生情况。结果 研究组干预 后VAS评分低于对照组（P ＜0.05）;研究组干预后SAS、SDS评分低于对照组（P ＜0.05）;研究组干预后 瘢痕厚度、色泽、血管分布、柔软度评分低于对照组（P ＜0.05）;研究组干预后躯体功能、心理功能、 社会关系、一般健康状况评分高于对照组（P ＜0.05）;研究组并发症发生率为5.00%,低于对照组的 25.00%（P＜0.05）。结论 在使用湿润烧伤膏进行创面修复的深度烧伤患者中采用综合护理的效果较好,能 够缓解患者疼痛,改善其负性情绪及瘢痕情况,进而提高其生活质量,减少并发症发生,值得临床应用。     

心形减张美容缝合术对剖宫产患者切口愈合的效果

目的 分析剖宫产患者应用心形减张美容缝合术对切口愈合的影响。方法 选取2021年5月-2023年 5月永靖县妇幼保健院收治的60例剖宫产患者为研究对象,随机分成对照组和研究组,每组30例。对照组采用常 规缝合方法,研究组采用心形减张美容缝合术,比较两组切口愈合时间、切口缝合时间、疼痛情况、并发症发生 情况、瘢痕美观程度。结果 研究组切口缝合以及愈合时间均短于对照组（P ＜0.05）;研究组切口愈合程 度及瘢痕美观度均优于对照组（P ＜0.05）;研究组术后7、14 d疼痛评分低于对照组（P＜0.05）;研究组 并发症发生率为3.33%,低于对照组的20.00%（P＜0.05）。结论 心形减张美容缝合术可促进剖宫产手术患 者术后伤口愈合,抑制瘢痕形成,提高皮肤美观程度,降低术后疼痛感,且并发症发生率较低。     

VSD技术联用表皮生长因子对深度烧伤创面愈合及炎症应激反应的影响

探讨VSD技术联用表皮生长因子对深度烧伤创面愈合及炎症应激反应的影响。方法 选取 2022年11月-2023年11月我院收治的深度烧伤患者62例,随机分为对照组和观察组,各31例。对照组应 用VSD技术与生理盐水冲洗治疗,观察组在对照组基础上应用表皮生长因子治疗,比较两组创面愈合 情况和炎症应激反应情况。结果 观察组创面愈合时间短于对照组,瘢痕生成数及感染数低于对照组 （P＜0.05）;观察组IL-1、TNF-α、E、NE、R水平低于对照组（P＜0.05）。结论 VSD技术结合表皮生 长因子能促进深度烧伤患者的创面愈合,提高创面愈合效果,减轻患者炎症应激反应。     

早期整形修复对手部烧伤后瘢痕挛缩畸形创面愈合及关节功能的影响

目的 分析早期整形修复对手部烧伤后瘢痕挛缩畸形创面愈合及关节功能的影响。方法 选取胜 利油田中心医院2020年1月-2023年10月收治的70例手部烧伤后瘢痕挛缩畸形患者为研究对象,随机分为 对照组与观察组,各35例。对照组予以常规治疗联合整形修复治疗,观察组予以常规治疗联合早期整形 修复治疗,比较两组创面愈合情况、关节功能、治疗满意度及生活质量。结果 观察组术后7、15、30 d 创面面积均小于对照组（P ＜0.05）;观察组术后15、30 d手关节活动度量表（TAM）评分均高于对照 组（P ＜0.05）;观察组治疗满意度为97.14%,高于对照组的62.86%（P ＜0.05）;观察组术后生活质量评 分高于对照组（P ＜0.05）。结论 对手部烧伤后瘢痕挛缩畸形患者实行早期整形修复有利于创面愈合, 促进关节功能恢复,可提高患者治疗满意度,改善生活质量。     

Experimental Studies on the Effect of Lidocaine on Wound Healing

p = 0.120). Important statistical differences were observed in vascularity ( p < 0.003) and morphometric results ( p < 0.001), where collagen was found in small amounts in the lidocaine group. The results of this study suggest that local infiltration of lidocaine produces significant histopathologic changes, but it does not substantially alter wound healing as there were no differences in the breaking strength of the wounds.