磁性靶向顺铂白蛋白微球的研究

将顺铂和磁性氧化铁共包于白蛋白中而制成了磁性靶向顺铂白蛋白微球。按正交设计筛选最佳合成工艺为：顺铂用量为４０ｍｇ,白蛋白用量为２５０ｍｇ,ＦｅＣｌ２用量为２８２．８ｍｇ,ｐＨ＝１０。并以微球进行了质量考察：微球平均粒径为２．８９±０．５１μｍ,磁性靶向顺铂微球的载药量为１３．６４％（ｍｇ／ｍｇ）,包裹率为８５．２４％;含药微球在体外１２ｈ释放完全,具一定的缓释作用;稳定性考察表明所制微球稳定性良好。     

磁性聚乙烯醇缩丁醛微球固定化α-淀粉酶

制备出磁性聚乙烯醇缩丁醛微球,并用该微球做载体,采用共价交联法固定α 淀粉酶。最佳固定化工艺条件为:pH=6 07,激活和交联时戊二醛的质量分数分别为4%和0 025%。在最佳固定化条件下所制磁性固定化酶的活力为25426 3U/g微球,蛋白载量为187 2mg/g微球,比活为135 8U/mg蛋白,活性回收率为36 9%。磁性固定化酶的理化性质为:磁性固定化酶的最适温度(60℃)比自由酶(50℃)高,最适pH(6 97)与自由酶相同,磁性固定化酶Km(米氏常数)值(5 7×10-4kg/L)较自由酶Km值(5 0×10-4kg/L)大,热稳定性、pH稳定性及操作稳定性均比自由酶有所提高。     

磁性Bi_2WO_6-Fe_3O_4复合微球的制备及吸附性能研究

用水热法合成了类花状结构的Bi_2WO_6-Fe_3O_4磁性微球。SEM和TEM照片显示复合微球由直径约为260 nm的Fe_3O_4颗粒镶嵌于直径为2~3μm的Bi_2WO_6微球体上。BET表征显示其比表面积为20.12 m~2/g,具有明显的介孔结构。VSM表征显示其磁饱和强度为6.1 emu/g,具有快速的磁响应性能。探讨了Bi_2WO_6-Fe_3O_4磁性微球对甲基橙的吸附性能,表明在25℃条件下对甲基橙(20 mg/L)的去除率为91.64%,吸附动力学遵循准二级动力学方程,吸附等温线符合Langmuir方程,最大饱和吸附量为20.67 mg/g。     

壳聚糖磁性微球的制备及其对牛血清白蛋白的吸附性能研究

以Fe/C为磁性内核、液体石蜡为分散介质、Span-80为乳化剂、环氧氯丙烷为交联剂,采用反相悬浮包埋法制备了壳聚糖磁性微球.对微球表面的活性基团含量进行了测定.研究了用戊二醛活化、Cibacron Blue 3G-A修饰后的微球对牛血清白蛋白的吸附性能.结果表明,小粒径壳聚糖磁性微球经戊二醛活化后对牛血清白蛋白的饱和吸附量为46.3 mg·g-1,经Cibacron Blue 3G-A修饰后对牛血清白蛋白的饱和吸附量为66.2 mg·g-1.     

改良热固法制备白蛋白微球的工艺研究

采用改良的热固化法制备了白蛋白微球,研究了乳化时搅拌速度和混合乳化剂对微球粒径大小的影响。结果表明较适宜的乳化搅拌速度为3500r／min,所制得白蛋白微球呈球形,表面光滑,粒径范围小;采用Span-80和Tween-80混合乳化剂时制备得到的白蛋白微球,比用单一的乳化剂制得的微球效果好,白蛋白微球呈球形,粒度在0．2μm到几个微米之间。     

全氟丙烷人血白蛋白微球注射液配制方法的优化

目的优化全氟丙烷人血白蛋白微球注射液配制方法。方法采用不同辅料超声制备全氟丙烷人血白蛋白注射液,并检测微球的大小及稳定性。结果采用2%右旋糖酐-40溶液作为辅料的配方制备的微球,粒径大小最佳,放置6个月后,各项质量指标几乎无变化,稳定性较好。结论已优化了全氟丙烷人血白蛋白微球注射液的配制方法。     

双甘膦修饰磁性四氧化三铁纳米微球吸附DNA的研究

用双甘膦(PMIDA)修饰磁性四氧化三铁纳米微球(MNP)并负载Zn2+制得了PMIDA-Zn2+修饰磁性微球吸附剂。考察了吸附溶液的pH值、离子强度、吸附时间、吸附温度等因素对DNA吸附的影响。结果表明,当吸附剂用量为10mg、pH值为5.0、离子强度(NaCl浓度)为2.0mol.L-1、吸附时间为20min、吸附温度为35℃时,吸附率可达80%,吸附容量为21mg.g-1。被吸附的DNA用3.5%的氨水能完全洗脱。将PMIDA-Zn2+修饰磁性微球用于玉米DNA的提取,所得DNA纯度较高,效果令人满意。     

磁性复合微球处理水中氯仿的应用研究

采用磁性复合微球为吸附载体,将其应用于水体中氯仿的吸附研究,在此过程中以紫外吸光光度法就磁性复合微球粒径、表面性质对吸附性能的影响作了考察,最终选取粒径为1.5μm、表面富含胺基磁性的复合微球为最佳吸附材料。与此同时,利用CODCr法作为评价手段对磁性的复合微球吸附氯仿的处理工艺条件进行了优化,确定每处理质量浓度为74 mg/L的氯仿溶液2 mL需用磁性复合微球约1 mg,最佳吸附时间为4.0 h,磁性复合微球的富集时间为4.0 h,此时对氯仿的吸附效率大于83%。     

磁性淀粉微球药物载体的合成及表征

先合成表面接羟基的磁流体,再在表面包覆上一层可溶性淀粉,采用悬浮聚合法和分散聚合法交联聚合成球,制得表面带羟基的磁性复合微球。经红外光谱、扫描电镜及粒度分析。结果表明,悬浮聚合法合成效果好,磁性淀粉复合微球分散性好,粒径在16~120μm占77%,Fe3O4在微球中平均含量为2.55 mg/g,微球结构坚韧,抗水溶性好。     

磁性淀粉微球对Ni(Ⅱ)吸附性能的研究

研究了磁性淀粉微球对Ni(Ⅱ)的吸附性能。考查了在常温条件下,反应时间、Ni(Ⅱ)的初始浓度、磁性淀粉微球的用量等对吸附性能的影响。探讨了磁性淀粉微球对Ni(Ⅱ)的吸附热力学和吸附动力学行为。结果表明:Ni(Ⅱ)为80mg/L,磁性淀粉微球用量为30mg时,在常温下经过80min的振荡吸附,磁性淀粉微球对Ni(Ⅱ)饱和吸附量达到11.69mg/g;吸附热力学表明磁性淀粉微球对Ni(Ⅱ)的吸附行为符合Freundlich方程;磁性淀粉微球对Ni(Ⅱ)离子的吸附过程可用准一级和准二级动力学模型进行模拟,但更符合二级动力学方程。     

磁性琼脂糖复合微球的制备和性质

采用乳化复合技术制备出粒径为２０～３００ｎｍ、分散系数为０．０９０～０．６０１、Ｆｅ３Ｏ４含量（ｗ）为７．５％～６１．３％的具有磁核的琼脂糖复合微球。该微球呈珠形,在４～９０℃的水介质中形成均匀稳定的分散液,在０．０５Ｗｂ／ｍ２的弱磁场中具强磁响应性。制备微球的最佳条件是：琼脂糖用量１２．５～８７．５ｍｇ／ｍｌ,氯化亚铁用量１５～１２０ｍｇ／ｍｌ,ｐＨ＞１０。     

Synthesis of Core/Shell Magnetic Porous Microspheres for Lipase Immobilization

Magnetic porous hydrophobic microspheres were prepared by modified suspension copolymerization of methacrylate (MMA) and divinylbenzene (DVB) in the presence of oleic acid coated magnetite (Fe₃O₄), and the microspheres were used as biocarrier for the lipase immobilization. The results showed that the magnetic microspheres possessed spherical shape, core/shell structure, porous structure and high magnetic content, and the size and structure of magnetic microspheres had no significant changes after enzyme binding. The particle average size of microspheres was 66 μm, the magnetic content of microspheres was up to 31%, and the magnetization saturation values of the core/shell magnetic microspheres were measured at 300 K to be 11.02 emu g⁻¹. Lipase was immobilized on the magnetic porous carrier at up to 16.30 mg/g carrier. Activity and enantioselectivity of the immobilized lipase for the synthesis of R-HMPC acetate were investigated, indicating an interfacial activation of the enzyme after immobilization. Moreover, the pH dependency and operational stability of the immobilized lipase were studied, and they possess high stability and can be reused for ten cycles with loss 10% activity.     

Preparation and characterization of magnetic poly(styrene–glycidyl methacrylate) microspheres for highly efficient protein adsorption by two‐stage dispersion polymerization

Micrometer‐sized superparamagnetic poly(styrene–glycidyl methacrylate)/Fe₃O₄ spheres were synthesized by two‐stage dispersion polymerization with modified hydrophobic Fe₃O₄ nanoparticles, styrene (St), and glycidyl methacrylate (GMA). The morphology and properties of the magnetic Fe₃O₄–P (St‐GMA) microspheres were examined by scanning electron microscopy, transmission electron microscopy, vibrating sample magnetometry, thermogravimetric analysis, and attenuated total reflectance. The average size of the obtained magnetic microspheres was 1.50 μm in diameter with a narrow size distribution, and the saturation magnetization of the magnetic microspheres was 8.23 emu/g. The magnetic Fe₃O₄–P (St‐GMA) microspheres with immobilized iminodiacetic acid–Cu²⁺ groups were used to investigate the adsorption capacity and selectivity of the model proteins, bovine hemoglobin (BHb) and bovine serum albumin (BSA). We found that the adsorption capacity of BHb was as high as 190.66 mg/g of microspheres, which was 3.20 times greater than that of BSA, which was only 59.64 mg/g of microspheres as determined by high‐performance liquid chromatography. With a rather low nonspecific adsorption, these microspheres have great potential for protein separation and purification applications. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133, 43005.     

Synthesis of magnetic polymer microspheres and application for immobilization of proteinase of balillus sublitis

Core–shell composite magnetic polymer microspheres, containing a magnetic core and a polymer shell, were synthesized by dispersion copolymerization of styrene (St) and 2-hydroxyethyl methacrylate (HEMA) in the presence of magnetic oxide (Fe₃O₄) powder. The Fe₃O₄ powder was ultrasonically disperesed in poly(ethylene glycol) (PEG) and the affinity between the obtained superfine powder and the monomer and initator was improved. It shows that the dispersion medium and stabilizer system have a great effect on the diameter and dispersion parameter of microspheres. In the condition of controlling polymerization, the magnetic polymer microspheres containing surface ? OH groups, having 50–500 μm diameter and with better magnetic induction, were synthesized. The proteinase of Balillus sublitis was immobilized on magnetic polymer microspheres with an average diameter of 50–60 μm by covalent coupling. The magnetic immobilized proteinase shows an enzyme activity of 1000 U/g, the enzyme yields are usually 20–30 mg/g of carriers, and the activity retention is about 40%. The stability of the immobilized enzyme was obviously improved. © 1995 John Wiley & Sons, Inc.     

硅烷化胺基磁性微球的合成与表征

采用溶液聚合法制备具有良好悬浮性和磁响应性的硅烷化胺基磁珠,对胺基磁性微球的形貌、结构、悬浮稳定性和磁响应性进行表征.研究结果显示,硅烷化胺基磁性微球的平均粒径为15nm,粒径分布比较均匀,近似为球形的壳核结构,核为磁性基质,壳为3-胺基丙基三乙氧基硅烷;将硅烷化胺基磁珠用于悬浮稳定性研究表明,磁微球具有较好的悬浮稳定...     

Preparation of superparamagnetic immunomicrospheres and application for antibody purification

A novel and effective protocol for the preparation of superparamagnetic immunomicrospheres has been developed. First, micro‐size magnetic poly (methacrylate‐divinylbenzene) (PMA‐DVB) spheres were prepared by a modified suspension polymerization method. The oleic acid coated magnetite (Fe₃O₄) nanoparticles made by coprecipitation were mixed with monomers of MA, DVB, and initiator benzoyl peroxide (BPO) to form oil in water emulsion droplets with the presence of poly (vinyl alcohol) (PVA‐1788) as a stabilizer. The polymerization reaction was carried out in a 2‐L beaker equipped with four vertical stainless steel baffleplates by increasing the temperature of the mixture at a controlled rate. The resulting magnetic microspheres are micro‐sized (less than 8μm in diameter) and 80 percent of them are in the size ranging from 1 to 5 μm. Then, they were highly functionalized via ammonolysis reaction with ethylenediamine, and the surface amino‐modified magnetic microspheres were obtained. The morphology and properties of these magnetic microspheres were examined by SEM, TEM, VSM, and FT‐IR. Affinity ligand protein A (ProtA) was covalently immobilized to the amino‐modified magnetic microspheres by the glutaraldehyde method. These ProtA‐immobilized magnetic immunomicrospheres were effective for affinity bioseparation processes, as was demonstrated by the efficient immunoaffinity purification of antibodies IgG2a (22mg per gram of microspheres) from mouse ascites. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 94: 2205–2211, 2004     

分散聚合法合成含有环氧基的无孔超顺磁性微球及其表征

Non-porous superparamagnetic polymer microspheres with epoxy groups were prepared by dispersion polymerization of glycidyl methacrylate (GMA) in the presence of magnetic iron oxide (Fe3O4) nanoparticles coated with oleic acid. The polymerization was carried out in the ethanol/water medium using polyvinylpyrrolidone (PVP) and 2,2'-azobisisobutyronitrile (AIBN) as stabilizer and initiator, respectively. The magnetic microspheres obtained were characterized with scanning electron microscopy (SEM), vibrating sample magnetometry (VSM) and Fourier transform infrared spectroscopy (FTIR). The results showed that the magnetic microspheres had an average size of 1μm with superparamagnetic characteristics. The saturation magnetization was found to be 4.5emu·g-1. There was abundance of epoxy groups with density of 0.028 mmol·g-1 in microspheres. The magnetic PGMA microspheres have extensive potential uses in magnetic bioseparation and biotechnology.     

磁性壳聚糖微球的合成及其在固定化血管紧张素转化酶的应用

以化学共沉淀法合成Fe3O4纳米粒子为磁核,采用乳化交联法制备磁性壳聚糖微球,并对其形貌、结构和磁饱和强度等性质进行了表征。以磁性壳聚糖微球作为载体,固定化猪肺粗提物中的血管紧张素转化酶,并对固定化条件进行研究。结果表明,固定化血管紧张素转化酶的最佳条件为：pH值为8.3,最佳温度为50 ℃,最佳时间为1.5 h,最佳酶溶液蛋白浓度为6 mg/mL,此时固定化酶活力最高为0.048 U/g微球。与游离酶相比,固定化酶的pH值稳定性和热稳定性均得到提高。固定化酶重复使用10次,仍然保持40%以上相对活力,说明磁性壳聚糖微球是固定化血管紧张素转化酶的良好载体。     

氨基和羧基功能化磁性微球去除水溶液中Cd(Ⅱ)和Pb(Ⅱ)

以甲基丙烯酸和丙烯酰胺为功能单体,通过悬浮聚合法制备了氨基和羧基双功能化的磁性复合微球(Fe3 O4@SiO2-NH2/COOH),并探讨了其对水溶液中Cd(Ⅱ)和Pb(Ⅱ)的吸附性能.X-射线衍射(XRD)分析表明,制备的磁性吸附剂内核为Fe3 O4.红外光谱(FT-IR)和扫描电镜(SEM)测试表明,氨基和羧基对Fe3 O4@SiO2表面改性成功.吸附试验显示,Fe3O4@SiO2-NH2/COOH吸附Cd(Ⅱ)和Pb(Ⅱ)的最优pH值分别为5.0和5.5,吸附过程均符合动力学准二级模型和Langmuir吸附等温模型,吸附剂对Cd(Ⅱ)和Pb(Ⅱ)最大吸附量分别为207.807 mg/g和168.995 mg/g.实际饮用水样中Cd(Ⅱ)和Pb(Ⅱ)的吸附表明,去除率分别可达97.74％和91.44％.该磁性吸附剂对两种重金属离子吸附量大、去除率高,具有良好的实际应用潜力.