出入境检验检疫领域中植物DNA条形码技术的应用探究

DNA生物技术的发展为DNA条形码技术的应用创造了条件,作为一项新技术,应用DNA片段来检查不同物种的类别,从而为出入境的植物检疫检验提供科学的支持。本文首先分析了DNA条形码技术的特征,在检疫中的应用以及实际发展中存在的问题,并提出了科学解决对策。     

基于条形码的采购管理

将条形码技术应用于采购管理,利用广泛应用在工业领域的39码,设计了条形码采购信息编码库.在此基础上,建立了条形码采购管理信息系统,利用SQL Server和Delphi技术,给出了条形码采购管理信息系统的具体实现.     

RFID技术在物流管理中的应用

RFID是一种新兴的自动识别技术,其优越的性能特点使其将取代条形码,并将在物流领域发挥重要作用.本中介绍了RFID技术及其特点,分析了RFID技术在物流管理各个环节中的应用,并提出RFID技术未来的研究方向.     

异域防伪

美国批准使用DNA条码防止海鲜以次充好美国进行的一项研究发现,鱼类产品误贴标签的现象非常普遍,受调的鱼类产品中有四分之一与广告宣传不符。庆幸的是,借助于DNA条形码技术,消费者购买鱼排或者鱼子酱等鱼类产品时将不再遭遇"挂羊肉,卖狗肉"的欺诈行为。日前,美国食品与药物管理局正式批准使用DNA条形码,防止出现     

编码与证卡防伪

1.条形码。本世纪40年代末,美国IBM公司有人开始研究同心圆形(所谓牛眼)代码标识,用于商品。到70年代逐步改进成条形码扫描技术并不断完善,得到国际标准ISO采用。 用于商品标识的肉限可见的条形码目前在我国已广为应用,其主要目的是为了产品识别、计价与管理。用无色防伪油墨印制的条形码,肉眼不可见,只在紫外线照射下方显可见荧光或紫外、极红荧光的条形码,也可     

在ERP系统中条形码输出输入方法的研究

针对条形码技术能够极大地提高劳动效率,而在实现条形码技术的过程中,如何实现条形码的打印输出是整个条形码使用过程中最重要的一个环节,着重论述了利用servlet、JasperReport和条形码字体3种实现条形码打印输出的方法.结合企业中ERP系统的实际情况,分析了选取条形码字体实现条形码打印输出的优势.并指出在实现条形码输入过程中需要注意的事项和关键点.     

浅谈条形码技术

随着计算机应用的不断普及,条形码的应用得到很大的发展.条形码可以标出商品的生产国、制造厂家、商品名称、生产日期、图书分类号、邮件起止地点、类别、日期等信息,因而在商品流通、图书管理、邮电管理、银行系统等许多领域都得到广泛的应用。     

条形码扫描

条形码在包装行业中正得到日益广泛的应用。某些领域如仓库存货盘存、充填生产线上容器的识别以及包装材料的处理加工等应用最普及。由于条形码应用变化很大,因此条形码设备也有很多型式。有若干类型的扫描装置和条形码阅读器,这些装置在特定的应用场合能产生良好的效果。但是,不论应用于什么方面,其实质在于具备适当的扫描装置以获得最佳效果。     

条形码技术在电能表资产管理及计量管理信息系统中的应用

主要介绍条形码技术在电能表资产管理及计量管理信息系统中的具体应用和实现,体现新技术与现代管理思想的有机结合,论述条形码技术的优越性。     

条形码技术在计量管理信息化中的应用

阐述了条形码技术在计量管理信息化应用中的必要性,并以所在单位进行试点,论证了计量管理中采用条形码技术所体现的信息技术优势。     

Flossing DNA in a Dual Nanopore Device

Solid‐state nanopores are a single‐molecule technique that can provide access to biomolecular information that is otherwise masked by ensemble averaging. A promising application uses pores and barcoding chemistries to map molecular motifs along single DNA molecules. Despite recent research breakthroughs, however, it remains challenging to overcome molecular noise to fully exploit single‐molecule data. Here, an active control technique termed “flossing” that uses a dual nanopore device is presented to trap a proteintagged DNA molecule and up to 100's of back‐and‐forth electrical scans of the molecule are performed in a few seconds. The protein motifs bound to 48.5 kb λ‐DNA are used as detectable features for active triggering of the bidirectional control. Molecular noise is suppressed by averaging the multiscan data to produce averaged intertag distance estimates that are comparable to their known values. Since nanopore feature‐mapping applications require DNA linearization when passing through the pore, a key advantage of flossing is that trans‐pore linearization is increased to >98% by the second scan, compared to 35% for single nanopore passage of the same set of molecules. In concert with barcoding methods, the dual‐pore flossing technique could enable genome mapping and structural variation applications, or mapping loci of epigenetic relevance.     

A taxonomy for simulation modeling based on programming language principles

Although many diverse areas employ simulation models, no agreed-upon taxonomy has been developed to categorize and structure simulation models for all science and engineering disciplines. The discipline of simulation is often splintered due to this lack of structure, with ad hoc model classes such as 'discrete event,' 'continuous' and 'combined.' These classes most often reflect the method of execution used on a model rather than the design structure of the model. We present a uniform model design taxonomy whose categories are inspired from categories in programming language principles within the field of computer science. The taxonomy includes a set of primitive model types (conceptual, declarative, functional, constraint, spatial) and a way of integrating primitive model types together (multimodeling). These model types are discussed using a single application: a robot server for an assembly line. We have found this taxonomy enables simulators to more easily define and categorize their models as well as to understand how model types from seemingly disparate application areas are interrelated.     

Shop floor control and tool loading policies in flexible manufacturing systems

We develop a classification scheme and taxonomy of operating decisions in flexible manufacturing systems (FMS). The taxonomy is used to identify a promising research area termed General FMSs (GFMS), where very little academic work has been reported to date in the prior literature. A decision framework for the development of tool loading and shop floor control policies in GFMS is also presented, and used to highlight specific future research directions. Strategic justification for developing operating policies for the GFMS classification is also provided.     

A human factors methodology for safety assessment based on the DYLAM approach

This paper discusses how the problem of human factors can appropriately be included in a probabilistic safety study. During incidents, the effect of the plant dynamic evolution and the failures of components can lead to non-intuitive configuration of the system and, when the interaction of the operator and the control and protection system is accounted for, the overall scenario of the safety study becomes particularly complex. These problems demand the use of a dynamic reliability methodology technique and the development of an appropriate model of human behavior to be coupled to a taxonomy of erroneous actions. At JRC these two endeavors have been carried out by the DYLAM approach and by a number of modeling architectures of operator simulation, which have been coupled for the development of a dynamic methodology for human factors analysis. In the paper, a simple application case is shown by the study of the control of an Auxiliary Feedwater System during incidental conditions.     

Color-tuned FRET polystyrene microspheres by single wavelength excitation

Fluorescent monodisperse polystyrene microspheres were prepared by two-stage dispersion polymerization, which successfully covalently labeled microspheres with two dyes without disturbing the final particle size and size distribution. By varying the dye concentrations, microspheres show tuned colors with different fluorescent intensity under a single wavelength excitation. Fluorescence resonance energy transfer (FRET) between two labeled dyes was proved to contribute to the emission of the longer-wavelength dye at a shorter-wavelength excitation. There is no dye leakage for microspheres because of the covalent incorporation of dye molecules. The microsphere matrix provides good protection of dye molecules and blocks the influence of media outside on the fluorescence of microspheres. Single microsphere shows intense fluorescence due to a large number of encapsulated dye molecules. These uniform barcoding fluorescent microspheres have potential application in multiplexed bioanalysis.     

发光细菌的研究和应用

发光细菌是一类在正常的生理条件下能够发射可见荧光的细菌，主要分布在海洋环境中。发光细菌及其发光基因在环境监测等领域中具有广泛的应用价值。本文综述了发光细菌的分类、发光机理及其应用。     

Pulsed field gel electrophoresis

The development of pulsed field gel electrophoresis has increased by 2 orders of magnitude the size of DNA molecules that can be routinely fractionated and analyzed. This increase is of major importance to molecular biology because it simplifies many previously laborious investigations and makes possible many new ones. Its range of application spans all organisms, from bacteria and viruses to mammals.     

Collection of trace amounts of DNA/mRNA molecules using genomagnetic nanocapturers

The collection and then the separation of rare DNA/mRNA targets with single-base mismatches in a complex matrix is critically important in human disease diagnostics, gene expression studies, and gene profiling. The major result of this work is the development and application of a novel genomagnetic nanocapturer (GMNC) for the collection, separation, and detection of trace amounts of DNA/RNA molecules with one single-base difference. The GMNC is constructed by bioconjugating molecular beacon DNA probes onto magnetic nanoparticle surfaces. We have successfully applied the GMNC in artificial buffer solution samples and in cancer cell samples, both containing different proteins and random DNA sequences. Our method has three distinctly useful features: highly efficient collection of trace amount of DNA/mRNA samples down to femtomolar (10(-15) M) concentrations; excellent ability to differentiate single-base-mismatched DNA/mRNA samples by combining the exceptional specificity of molecular beacons and the separation power of magnetic nanoparticles; and real-time monitoring and confirmation of the collected gene products. The newly developed genomagnetic nanocapturers will be highly useful for the collection of trace amounts of DNA/mRNA targets in a variety of sample sources in forensic, medical, and biotechnological fields.     

Bioconjugated silica nanoparticles: Development and applications

Advanced bioanalysis, including accurate quantitation, has driven the need to understand biology and medicine at the molecular level. Bioconjugated silica nanoparticles have the potential to address this emerging challenge. Particularly intriguing diagnostic and therapeutic applications in cancer and infectious disease as well as uses in gene and drug delivery, have also been found for silica nanoparticles. In this review, we describe the synthesis, bioconjugation, and applications of silica nanoparticles in different bioanalysis formats, such as selective tagging, barcoding, and separation of a wide range of biomedically important targets. Overall, we envisage that further development of these nanoparticles will provide a variety of advanced tools for molecular biology, genomics, proteomics and medicine. Electronic Supplementary Material  Supplementary material is available for this article at and is accessible for authorized users.