2种方法使用生物活性玻璃脱敏剂对牙本质小管封闭作用的场发射扫描电镜观察

目的使用场发射扫描电镜观察涂抹法和透明压膜托盘法使用生物活性玻璃脱敏剂在短时间内对牙本质小管的封闭效果,为增强生物活性玻璃的脱敏疗效提供新思路。方法制取56颗离体牙的牙本质片,6%柠檬酸脱矿后按处理方法和处理时间不同随机分为7组,涂抹法1 d组(A1组),涂抹法3 d组(A2组),涂抹法7 d组(A3组),压膜法1 d组(B1组),压膜法3 d组(B2组),压膜法7 d组(B3组),空白对照组。通过FESEM观察比较各组牙本质小管封闭效果。结果涂抹法和透明压膜托盘法使用生物活性玻璃脱敏剂后均能很好的封闭牙本质小管,牙本质小管内均可见明显矿化物沉积,A1、A2、A3、B1、B2、B3组牙本质小管的暴露率分别为(6.29±1.18)%、(3.40±0.84)%、(1.23±0.43)%、(5.31±0.97)%、(1.40±0.30)%、(0.36±0.15)%,A2组>B2组,A3组>B3组,差异有统计学意义;空白对照组牙本质小管口开放,周界清晰。结论透明压膜托盘法在短时间内可以增强生物活性玻璃对敏感牙本质小管的封闭效果,与涂抹法相比,封闭效果有提高,差异有统计学意义。     

生物活性玻璃促进敏感牙本质再矿化的体外研究

目的通过体外实验比较生物活性玻璃与两种常用抗敏方法之间对敏感牙本质的再矿化效果。方法制备35个离体牙的牙本质盘,随机分成5组(A~E),每组7个,A至D组用6%柠檬酸脱矿形成敏感牙本质模型,分别用生物活性玻璃(A)、氟离子导入(B)、氟化钠甘油(C)及蒸馏水(D)处理后浸于人工唾液中,E组不脱矿直接浸入人工唾液,各组均处理15天。然后,用扫描电镜(SEM)和X射线能谱分析(EDX),评估各组再矿化效果。结果 SEM观察发现A、B、C三组牙本质小管内均可见明显矿化物沉积,小管口封闭率A(98.65%±0.31%)>B(98.13%±0.35%)>C(91.50%±1.52%),A组与B组之间无显著性差异(P>0.05),但均显著高于C组(P<0.01);D、E两组牙本质小管口开放,周界清晰;小管口封闭率分别为D(6.23%±1.19%)和E(14.34%±1.03%)。EDX分析显示五组牙本质表面钙磷比无显著性差异(P=0.075)。结论生物活性玻璃与氟离子导入处理均对敏感牙本质有很好的促进再矿化效果。     

透明托盘法使用生物活性玻璃对牙髓腔渗透性的影响

目的将纳米级生物活性玻璃脱敏剂用于漂白治疗前的离体牙,观察比较未使用脱敏剂和分别用涂抹法和透明托盘法使用生物活性玻璃脱敏剂后牙髓腔内过氧化氢渗透量的多少。方法 48颗离体第一前磨牙分成6组,A组不做脱敏处理,牙冠倒置,冠部分浸泡于去离子水中30 min,B组表面不做脱敏处理,牙冠倒置于30%过氧化氢溶液中30 min,C组生物活性玻璃涂抹2 min,2次/d,浸没于人工唾液24 h后,牙冠倒置于30%过氧化氢溶液中,D组使用透明托盘+生物活性玻璃1 h,浸没于人工唾液24 h后,牙冠倒置于30%过氧化氢溶液30 min,E组生物活性玻璃涂抹2 min,2次/d,浸没于人工唾液24 h后,牙冠倒置于去离子水中30 min,F组使用透明托盘+生物活性玻璃1 h,浸没于人工唾液24 h后,牙冠倒置浸泡于去离子水中30 min,提取各组髓腔内液体,检测各组的吸光度,并换算成过氧化氢的总量。结果 6组髓腔内液过氧化氢含量分别为A组0μg,B组21.149μg±0.489μg,C组9.813μg±0.426μg,D组4.065μg±0.268μg,E组0μg,F组0μg,单因素方差分析表明,B、C、D组髓腔内的过氧化氢含量有差异,差异有统计学意义(F=459.748,P<0.05),B组>C组>D组。结论生物活性玻璃脱敏剂结合透明托盘的使用可以有效减少过氧化氢渗透进牙髓腔的量,降低过氧化氢对牙髓组织产生的有害影响。     

Er:YAG激光联合生物活性玻璃对封闭牙本质小管效果的研究

目的 研究Er:YAG激光联合生物活性玻璃脱敏剂对牙本质小管的封闭率和抗老化效果,探讨两者联合应用时的最佳顺序.方法 制作45个敏感牙本质模型,将样本随机分成5组,前四组平均每组10颗,应用不同方法进行牙本质脱敏处理:A:Er:YAG激光组;B:生物活性玻璃组;C:Er:YAG激光+生物活性玻璃组;D:生物活性玻璃+E...     

3种脱敏剂封闭牙本质小管的扫描电镜观察

目的:比较3种脱敏剂封闭牙本质小管的效果。方法:新鲜离体正畸牙48颗,随机分为4组(3组为实验组,1组为对照组),分别用Systemp脱敏剂(A组)、Seal&protectTM脱敏剂(B组)、Gluma脱敏剂(C组)对实验组暴露的牙本质表面进行处理。然后劈开离体牙处理面,用扫描电镜分别对牙本质表面和剖面进行观察,在组间进行即刻和3个月后(模拟)效果的比较,并与对照组进行比较。结果:3种脱敏剂均在牙本质表面形成封闭,A组和B组还在牙本质小管内形成“树脂突”样结构,且A组较深,而C组没有“树脂突”形成。结论:3种脱敏剂都能取得封闭牙本质小管的效果,Systemp较另两者的封闭性/耐久性可能更佳。     

仿生矿化纳米磷酸钙对牙本质小管封闭性能的评价

目的 制备仿生矿化的纳米磷酸钙,并探讨其对牙本质小管的封闭性能。方法 采用DMEM仿生矿化策略制备无定形磷酸钙材料(DMEM based amorphous calcium phosphate,DACP),运用透射电子显微镜(TEM)、傅里叶变换红外光谱扫描(FTIR)等检测观察其理化表征。选择口腔上皮角质形成细胞(HOK)和牙髓细胞(DPC)分别与材料共培养24 h后,CCK-8法评估材料的生物相容性。收集完整无龋的牙齿制备牙本质片,用DACP材料悬液均匀涂抹牙本质片,设置阳性对照NovaMin组和空白对照组,分别处理1、7 d后扫描电子显微镜(SEM)评估牙本质片的表面和截面封闭效果。结果 TEM显示DACP为均匀球形无定形纳米颗粒,随矿化时间的延长粒径有所增加。CCK-8结果显示HOK和DPC在25、50、100、200 μg/mL DACP下细胞活力均较好,提示材料有较好的生物相容性。牙本质片被处理1 d后DACP组牙本质小管大部分封闭;被处理7 d后DACP组牙本质小管表面呈现完全封闭的状态,且牙本质片截面可见DACP可渗入小管内。结论 运用DMEM仿生矿化策略制备的纳米磷酸钙具有较好的生物相容性和牙本质小管封闭作用,有望成为一种新的脱敏材料。     

生物活性玻璃与铒激光联用对磨切牙本质模型的封闭效果

目的研究生物活性玻璃与铒激光联用对磨切牙本质模型的封闭效果。方法制取45个离体牙的牙本质盘,面牙本质按粗磨、细磨、抛光的顺序打磨平整,构建磨切牙本质模型,按处理方法不同随机分为5组:空白对照组(A),生物玻璃组(B),Er,Cr:YSGG激光组(C),生物玻璃+Er,Cr:YSGG激光组(D),Er,Cr:YSGG激光+生物玻璃组(E)。通过扫描电镜(SEM)和其附属X射线能谱分析(EDXA)比较5组封闭牙本质小管效果及封闭物组成。结果 SEM观察发现B、C、D、E 4组绝大部分封闭物堵塞牙本质小管,4组的封闭效果均较好。小管面积暴露率:D(0.044%±0.003%)相似文献   

蜂胶醇封闭牙本质小管的扫描电镜观察

目的:研究不同浓度蜂胶醇封闭牙本质小管的效果,为蜂胶脱敏治疗提供形态学依据. 方法:将35个新鲜上颌前磨牙随机分为A、B、C、D、E、F、G7组,制作3㎜×3㎜×2㎜牙本质磨片,清洁,酸蚀.A、B、C组分别涂10、20、30 g/L的蜂胶醇,D组涂含氟涂膜,E组作为空白对照组,扫描电镜观察牙本质小管封闭情况,筛选蜂胶最佳浓度(记作X).F组涂抹浓度为X的蜂胶,G组涂含氟涂膜,用牙刷刷洗标本表面,扫描电镜观察牙本质小管封闭情况.结果:30 g/L的蜂胶醇能够完全封闭牙本质小管,且渗入沉淀达一定深度,经过刷牙后封闭效果没有明显减弱.结论:蜂胶对牙本质小管具有良好的封闭作用,在治疗牙齿感觉过敏症方面具有进一步研究价值.     

氟钾酚醛树脂脱敏剂封闭牙本质小管的扫描电镜观察

目的 通过扫描电镜观察,探讨新型脱敏剂氟钾酚醛树脂对牙本质小管的封闭作用及其抗洗刷能力,为其用于临床脱敏治疗提供理论依据.方法 收集36颗无龋坏、无缺损、新鲜离体正畸前磨牙,制备成牙本质盘标本,随机分为A、B、C3组,每组12颗,A组不做处理,B组和C组分别使用酚醛树脂液和氟钾酚醛树脂液对暴露的牙本质表面进行处理.每组取4颗牙进行刷牙实验,然后劈开离体牙,用扫描电镜分别对牙本质表面和纵剖面进行观察,并用Image-proplus 6.0图像分析软件量化分析.结果 B组和C组标本均能在牙本质表面形成有效封闭,2组间的牙本质小管面积及相对面积差异无统计学意义(P＞0.05),并且2组脱敏剂都能渗入牙本质小管内达一定深度,C组渗入深度明显大于B组(P＜0.05),刷牙试验后,C组牙本质表面的封闭性明显优于B组(P＜0.05).结论 酚醛树脂脱敏剂和氟钾酚醛树脂脱敏剂都能取得封闭牙本质小管的效果,而氟钾酚醛树脂脱敏剂的封闭性和抗洗刷能力更强.     

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目的评价含生物活性玻璃成分的脱敏剂与氟保护漆联合应用对牙本质小管的堵塞效果及治疗牙本质过敏症(dentin hypersensitivity,DH)的临床疗效。方法选择2013年6月到2014年6月到中国医科大学口腔医学院干诊科就诊的59例DH患者的120颗患牙,随机分为A组(41颗)、B组(39颗)、C组(40颗),分别使用氟保护漆、奥敏清及氟保护漆与奥敏清联合涂布敏感处,应用视觉模拟评分(visual analogue scale,VAS)法评价其疗效。另选择因正畸需要拔除的完整无龋坏前磨牙20颗,制备成敏感模型后随机分为a、b、c、d四组,每组5颗,a、b、c三组处理方法同上述A、B、C组,d组为空白对照组,用蒸馏水进行处理,扫描电镜观察各组牙本质小管的堵塞情况。结果 A、B、C三组的VAS评分值在处理即刻均明显下降,且随时间推移均有增高趋势;有效率比较,A、C两组在1个月时差异有统计学意义(P<0.05),其他各组间在各时点差异均无统计学意义(P>0.05);各组内比较,A组即刻与1个月时差异具有统计学意义(P<0.05)。扫描电镜观察a、b、c三组牙本质小管均有不同程度堵塞,其中以联合应用组效果最佳。结论含生物活性玻璃成分的脱敏剂与氟保护漆联合应用堵塞牙本质小管的效果最佳,且具有较好的临床效果。     

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目的探讨Er:YAG激光与Gluma脱敏剂联合应用对牙本质小管的封闭作用。方法选取2010年3—6月于山西医科大学口腔医院口腔颌面外科因正畸拔除的24颗前磨牙,制成厚约2mm的牙本质片。根据其表面处理方式的不同随机分为4组:对照组、Er:YAG激光组、Gluma脱敏剂组、联合应用组(Er:YAG激光联合Gluma脱敏剂),每组6颗前磨牙。处理后,扫描电镜观察各组牙本质小管形态特征,并测量牙本质小管口的直径和相对面积。结果联合应用组中牙本质小管口的直径和相对面积均明显小于其他各组,且差异有统计学意义(P<0.05)。结论 Gluma和Er:YAG联合应用对牙本质小管的封闭作用优于单独应用Gluma脱敏剂或Er:YAG激光。     

脱敏剂MS-COAT阻塞牙本质小管的电镜观察分析

[摘要] 目的 通过场发射扫描电镜观察脱敏剂MS-COAT对牙本质小管的堵塞效果,分析其对于治疗牙本质过敏症的机理。方法 制作牙本质小管暴露模型,并通过扫描电镜观察使用MS-COAT脱敏剂组和空白对照组的牙本质小管表面情况。同时通过能谱分析拍摄扫描电镜图片,对牙本质小管堵塞物成分进行分析。结果 MS-COAT处理组横断面牙本质小管大部分被晶体类物质封闭,纵断面可见牙本质小管内有堵塞物。对堵塞物及牙本质成分进行能谱分析:堵塞物主要成分为钙和磷,牙本质含钙磷量97.44%,堵塞物含钙磷量较低结论 MS-COAT脱敏剂能有效封闭牙本质小管表面和内部,具有较好的脱敏的作用。     

Occluding effect of Nd:YAG laser and different dentin desensitizing agents on human dentinal tubules in vitro: a scanning electron microscopy investigation

OBJECTIVES : This in vitro study aimed to microscopically evaluate and compare the occluding effect of the Nd:YAG laser and different dentin desensitizing agents on human dentinal tubules. MATERIALS AND METHODS : The Nd:YAG laser (SunLase? 800) and four commercially available and professionally applied dentin desensitizers (Gluma? desensitizer, Tenure Quick?, Quell? desensitizer, and VivaSens?) were investigated in this study. Sixty-four extracted intact human molars were used. Each dentin surface was divided by shallow indentation into two halves, one of which was used for treatment and the other of which served as a control. The dentin surfaces were etched to remove any smear plugs and to mimic the open dentinal tubules of sensitive dentin using 0.5 M ethylenediaminetetraacetic acid (pH 7.4) for two minutes (applied with a microbrush) and then rinsed with an air-water syringe for 30 seconds. The laser samples (n=16) were randomly divided into four groups of four samples each. These groups were the step-up technique group, the 14-day group, the one-minute group, and the two-minute group. Forty-eight samples were treated with the four tested desensitizing agents and were randomly divided into four groups (n=12/group). Each group was further subdivided into three subgroups (n=4). Samples of the first subgroup were treated for 14 days, while those of the second subgroup were treated once. Samples of the last subgroup were fractured longitudinally after a single treatment. All of the samples were then examined under a scanning electron microscope. RESULTS : The Nd:YAG laser-irradiated dentin showed reduction or complete obliteration of the dentinal tubule lumen; thus, the treatment modified the original dentinal structure. The lased dentin surface in the two-minute group showed bubble-like changes in the area of the dentinal tubules' orifices. Statistically, the two-minute group was found to have a significantly higher percentage of partially or fully occluded tubules than did the one-minute group. All of the studied desensitizing agents produced occlusion of the dentinal tubules; however, the appearance of the precipitates, the level of coverage, and the degree of dentinal occlusion varied among the tested products. CONCLUSION : Throughout the specified period of this study, occlusion and/or narrowing of the open dentinal tubules have been successfully achieved with both treatment approaches.     

A novel bioactive glass-ceramic for treating dentin hypersensitivity

Dentin hypersensitivity (DH) is a painful response to stimulus applied to the open dentinal tubules of a vital tooth. It's a common oral condition, however, without an ideal treatment available yet. This work evaluated in vitro the effect of micron-sized particles from a novel bioactive glass-ceramic (Biosilicate) in occluding open dentinal tubules. A dentin disc model was employed to observe comparatively, using scanning electron microscopy (SEM), dentinal tubule occlusion by different products and deposition of hydroxyl carbonate apatite (HCA) on dentin surface by Biosilicate, after a single application: G1 - Dentifrice with potassium nitrate and fluoride; G2 - Two-step calcium phosphate precipitation treatment; G3 - Water-free gel containing Biosilicate particles (1%); G4 - Biosilicate particles mixed with distilled water in a 1:10 ratio; all of them after 1, 12 and 24 hours of immersion in artificial saliva. Fourier transform infrared spectroscopy (FTIR) was performed to detect HCA formation on dentin discs filled with Biosilicate after 2 minutes, 30 minutes and 12 hours of immersion in artificial saliva. SEM showed a layer of HCA formed on dentin surface after 24 hours by G4. G1, G2 and G3 promoted not total occlusion of open dentinal tubules after 24 hours. FTIR showed HCA precipitation on the dentin surface induced by Biosilicate after 30 minutes. The micron-sized particles from the bioactive glass-ceramic thus were able to induce HCA deposition in open dentinal tubules in vitro. This finding suggests that Biosilicate may provide a new option for treating DH.     

含氯化锶脱敏糊剂对牙本质小管的封堵效果及生物安全性评价

目的 研究含2%氯化锶的脱敏剂对牙本质小管的封堵效果,并对其生物安全性进行评价。方法 制备含2%十二烷基硫酸钠(SLS)和2%氯化锶的脱敏糊剂(DS1)。选择无龋坏无缺陷的牛下颌切牙制备牙本质片24个,随机分3组,分别置于DS1(实验组)、普通含氟牙膏(阴性对照组)和去离子水(空白对照组)中进行刷牙处理。牛牙表面连续处理7天后采用扫描电子显微镜(SEM)及其图像分析研究含锶脱敏糊剂对牙本质小管的封堵率。另外,选择市售含氟牙膏(阴性对照)、不含SLS的脱敏糊剂(DS2)及3种同类脱敏产品,采用滤膜扩散法进行体外细胞毒性研究,并评价脱敏糊剂DS1浸提液的口腔黏膜刺激反应和皮肤致敏反应。采用一次限量法研究脱敏糊剂的急性经口毒性(LD₅₀)。结果 SEM分析表明,DS1对牙本质小管的封堵率为70.97%,阴性对照组为48.29%,两者之间及相比空白对照组,差异均具有统计学意义(P<0.05)。DS1具有一定的细胞毒性,但未产生刺激和致敏反应。DS1的LD₅₀大于5 000 mg/kg,但呈现实际无毒的结果。结论 在本研究中,含有2%的SLS...     

SEM investigations on the human sclerosed dentinal tubules

Sclerotic changes of dentinal tubules appeared in the dentin beneath the carious, attrition and abrasion lesions and in the transparent root dentin were investigated using the scanning electron microscopy. 1) Shape of crystal-like substances appeared in the dentinal tubules could not be closely related to kinds of dentin destruction. 2) In the dentinal tubules beneath the carious lesions, cuboidal or rhomboid-shaped, short rod-like, long rod-like, plate-like and droplet-like crystals were mainly observed. Combination of cuboidal or rhomboid-shaped and short rod-like crystals or that of long rod-like and plate-like crystals were most frequently observed. 3) In the dentinal tubules beneath attrition and abrasion lesions, cuboidal or rhomboid-shaped, short rod-like and droplet-like crystals were mainly observed, however, the size of cuboidal or rhomboid-shaped crystal was usually smaller than that observed beneath carious lesion and they deposited compactly within the tubules. 4) In the sclerosed dentinal tubules in the transparent root dentin, compact deposition of rhomboid-shaped and irregular sand-like crystals were usually observed. Size of rhomboid-shaped crystal is smaller than that observed beneath carious lesion. The deposit of sand-like crystal in the tubules was easily discriminated from the matrix of peritubular dentin. 5) In any case, the kinds of crystals and pattern of deposition were not necessarily same between the neighboring tubules. However, in a tubule, they were almost same in shape and mode of deposition throughout the entire length of tubule from the outer side to the pulpal side. These facts seem to indicate that mineral deposition within the tubule due to sclerosis is performed under individual condition. 6) Finding showing biological control of mineral deposition within the dentinal tubules by the odontoblasts could not be observed.     

Systemp脱敏剂治疗牙本质过敏症的研究

目的观察Systemp脱敏剂治疗牙本质过敏症的效果.方法牙本质过敏症患牙240颗,实验组120颗,使用Systemp脱敏剂,对照组120颗使用30%草酸钾溶液.离体牙30颗,磨除牙冠颈1/3区牙釉质,暴露牙本质,分3组,第1组涂布Systemp,第2组涂布草酸钾溶液,第3组不作任何处理,进行扫描电镜观察.结果实验组与对照组的疗效存在显著性差异,各组内随着时间的推移,疗效出现下降且存在显著性差异.电镜下可见,经Systemp处理后牙本质表面的牙本质小管基本被封闭,开口不显现,并有"树脂突"样结构深入牙本质小管约50μm.结论Systemp能有效地封闭牙本质小管,治疗牙本质过敏症效果明显,持续性较佳,是一种较为理想的牙本质脱敏剂.     

The combined occluding effect of sodium fluoride varnish and Nd:YAG laser irradiation on human dentinal tubules.

Various methods and materials used in the treatment of dentin hypersensitivity are thought to achieve a therapeutic benefit by tubule occlusion. The aim of the present study was to evaluate the combined occluding effect of sodium fluoride varnish and Nd:YAG laser irradiation on human dentinal tubules. Thirty-six dentin specimens with exposed dentinal tubule orifices were used in this study. The samples were randomly divided into four groups. Groups A, B, and C were varnished by sodium fluoride, whereas group D served as a control. Then, group C was lased by 30 mJ of Nd:YAG laser, 10 pulses/s for 2 min by light painting. Three hours later, groups B and C were brushed by an electrical toothbrush for 30 min. Under SEM observation, the control group showed numerous exposed dentinal tubule orifices, and the sodium fluoride varnished specimens showed closure of exposed dentinal tubule orifices. After electrical toothbrushing, most of the sodium fluoride varnish was brushed away, except in the specimens that were irradiated by Nd:YAG laser. Over 90% of the dentinal tubule orifices were occluded by sodium fluoride varnish combined with Nd:YAG laser irradiation.