共查询到20条相似文献,搜索用时 0 毫秒
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A human lymphoid-specific transcription factor that activates immunoglobulin genes is a homoeobox protein 总被引:86,自引:0,他引:86
C Scheidereit J A Cromlish T Gerster K Kawakami C G Balmaceda R A Currie R G Roeder 《Nature》1988,336(6199):551-557
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Tyrosine phosphorylation of vav proto-oncogene product containing SH2 domain and transcription factor motifs. 总被引:30,自引:0,他引:30
B Margolis P Hu S Katzav W Li J M Oliver A Ullrich A Weiss J Schlessinger 《Nature》1992,356(6364):71-74
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A transcription factor response element for gene expression during circadian night 总被引:26,自引:0,他引:26
Ueda HR Chen W Adachi A Wakamatsu H Hayashi S Takasugi T Nagano M Nakahama K Suzuki Y Sugano S Iino M Shigeyoshi Y Hashimoto S 《Nature》2002,418(6897):534-539
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了解转录因子GATA-2基因在ANLL中的表达和突变情况,方法:采用RT-PCR检测85例ANLL病人外周血单个核细胞中GATA-2基因的表达情况,PCR产物进一步经单链构象多态性(SSCP)分析以了解基因突变情况。结果:绝大多数ANLL都表达了GATA-2基因(89.4%),SSCP分析发现一例M2型的PCR产物出现异常迁移带,核苷序列分析显示在GATA-2基因第892位的核苷酸出现点突变,即第 相似文献
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Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor 总被引:3,自引:0,他引:3
Tumour necrosis factor (TNF) was found originally in mouse serum after intravenous injection of bacterial endotoxin into mice primed with viable Mycobacterium bovis, strain Bacillus Calmette-Guerin (BCG). TNF-containing serum from mice is cytotoxic or cytostatic to a number of mouse and human transformed cell lines, but less or not toxic to normal cells in vitro. It causes necrosis of transplantable tumours in mice. TNF also occurs in serum of rat, rabbit and guinea pig. Rabbit TNF has been purified recently to give a single band on SDS-polyacrylamide gel electrophoresis (PAGE). The purified TNF had a relative molecular mass (Mr) 40,000 +/- 5,000 measured by gel filtration, and 17,000 by SDS-PAGE. Its isoelectric point is 5.0 +/- 0.3. The necrotic activity in vivo and the cytotoxicity in vitro are produced by the same substance. The gene encoding TNF has been identified in a human genomic DNA library using as a probe a cloned cDNA encoding a portion of rabbit TNF. The regions of this gene encoding an amino-acid sequence corresponding to mature TNF have been expressed in Escherichia coli and the product of this expression isolated in pure form and shown to produce necrosis of murine tumours in vivo. 相似文献
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Characterization of the human factor VIII gene 总被引:21,自引:0,他引:21
J Gitschier W I Wood T M Goralka K L Wion E Y Chen D H Eaton G A Vehar D J Capon R M Lawn 《Nature》1984,312(5992):326-330
The complete 186,000 base-pair (bp) human factor VIII gene has been isolated and consists of 26 exons ranging in size from 69 to 3,106 bp and introns as large as 32.4 kilobases (kb). Nine kb of mRNA and protein-coding DNA has been sequenced and the mRNA termini have been mapped. The relationship between internal duplications in factor VIII and evolution of the gene is discussed. 相似文献
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Structure and evolution of a human erythroid transcription factor 总被引:44,自引:0,他引:44
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Activation of BPV-1 replication in vitro by the transcription factor E2. 总被引:53,自引:0,他引:53
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为了构建人Annexin A2基因真核表达载体。通过TRIzol法提取Hela宫颈癌细胞中总RNA,采用RT-PCR技术(逆转录-聚合酶链反应)扩增Annexin A2的基因片段,并成功构建成VR1012-Annexin A2-HA重组质粒。经酶切、测序检测其构建的正确性,将质粒瞬时转染到293T人胚肾细胞,使用免疫荧光和蛋白印迹(Western blot)法检测基因表达,使用流式细胞术检测转染后细胞凋亡率。结果表明实验成功构建了具有表达活性的Annexin A2真核表达质粒,为进一步研究Annexin A2的抗凋亡作用奠定了基础。 相似文献