糖尿病患者血清microRNA-152表达水平与血脂代谢、胰岛素抵抗的相关性

目的 探讨糖尿病患者血清microRNA-152（miR-152）表达水平与血脂代谢、胰岛素抵抗的相关性。方法 选取2018年1月—2018年11月在攀枝花市中西医结合医院就诊的血糖异常患者109例,分为2型糖尿病（T2DM）前期组（Pre-T2DM组）45例和T2DM组64例;另取同期该院门诊部健康体检者70例为对照组。检测血清空腹胰岛素（FINS）、总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、空腹血糖（FBG）水平,评估稳态胰岛素评价指数（HOMA-IR）与胰岛β细胞功能指数（HOMA-β）;并分析miR-152与血脂水平及FBG、FINS、HOMA-IR、HOMA-β的相关性。结果 与对照组相比,Pre-T2DM组、T2DM组患者血清miR-152、FBG、FINS、TG、TC、LDL-C、HOMA-IR较高（P <0.05）,HDL-C、HOMA-β较低（P <0.05）;与Pre-T2DM组相比,T2DM组患者血清miR-152、FBG、FINS、TG、TC、LDL-C、HOMA-IR较高（P <0.05）,HDL-C、HOMA-β较低（P <0.05）。T2DM患者miR-152与TG、TC、LDL-C、FBG、FINS、HOMA-IR呈正相关（r =0.844、0.755、0.833、0.528、0.576和0.635,均P <0.05）,与HDL-C、HOMA-β呈负相关（r =-0.655和-0.603,均P <0.05）。多因素一般Logistic回归分析结果显示,血清FBG ［R=2.305（95% CI：1.369,3.882）］和miR-152 ［R =2.831（95% CI：1.736,4.618）］升高是影响胰岛素抵抗的危险因素。结论 T2DM患者血清miR-152上调,其可能通过调节血脂代谢影响T2DM病情发展。     

西格列汀对肥胖大鼠血清内脏脂肪素水平的影响研究

目的 观察西格列汀对肥胖大鼠代谢指标及血清内脏脂肪素水平的影响。方法 40只SD大鼠随机分为普通饲料喂养组(ND组,n=10)和高脂饲料喂养组(HD组,n=30)。16周后HD组成功建立肥胖模型(n=16),并随机分为肥胖对照组(OB组,n=8)和西格列汀干预组(SITA组,n=8)。分别测定干预前后各组大鼠体质量(BW)、空腹血糖(FBG)、甘油三酯(TG)、总胆固醇(TCH)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、空腹胰岛素(FINS)及血清内脏脂肪素水平,计算胰岛素抵抗指数(HOMA-IR)。结果 干预前,HD组的BW、FBG、TG、TCH、LDL-C、FINS、HOMA-IR、内脏脂肪素高于ND组,HDL-C低于ND组,差异有统计学意义(P<0.05)。干预后,SITA组FBG、TG、TCH、LDL-C、FINS、HOMA-IR低于OB组,HDL-C高于OB组,差异有统计学意义(P<0.05)。血清内脏脂肪素与BW、FBG、FINS、HOMA-IR、TCH、LDL-C呈正相关,与HDL-C呈负相关(P<0.05)。结论 高脂饮食诱导肥胖大鼠模型出现糖脂代谢紊乱及胰岛素抵抗,血清内脏脂肪素水平明显增加,西格列汀能下调血清内脏脂肪素水平,改善糖脂代谢,减轻胰岛素抵抗。     

孕妇血清FABP4 PTEN及新生儿脐血中FABP4在妊娠期糖尿病患者中的表达

目的 探讨孕妇血清、新生儿脐血中FABP4与血清PTEN在妊娠期糖尿病患者中的表达。方法 选取2014年1月至12月保定市妇幼保健院收治的妊娠期糖尿病孕妇30例为妊娠期糖尿病（GDM）组,选取同期正常妊娠孕妇30例对照组。临产前检测两组孕妇血清PTEN、FABP4及其两组新生儿脐血中FABP4的水平,空腹血糖（FBG）、血清胰岛素（FINS）、三酰甘油（TG）、总胆固醇水平;计算胰岛素抵抗指数（HOMA-IR）、胰岛素敏感指数（HOMA-ISI）,分析比较两组对象上述指标,采用Pearson相关分析法分析PTEN、FABP4、HOMA-IR、新生儿体质量、孕妇血脂水平、孕前BMI相关性。结果 GDM组孕妇临产前BMI、TG、FBG、FINS、HOMA-IR、HOMA-ISI、FABP4、PTEN、新生儿体质量与对照组比较,差异有统计学意义（P<0.05）。血清PTEN与血清FABP4、HOMA-IR存在正相关（P<0.05）,血清FABP4与HOMA-IR存在正相关（P<0.05）。GDM组孕妇血清及新生儿脐血FABP4与新生儿体质量呈正相关（P<0.05）。结论 FABP4、PTEN参与妊娠期糖尿病的发展,在孕期产生胰岛素抵抗过程中发挥了重要的作用。     

妊娠糖尿病患者血清Betatrophin、Omentin-1水平与糖脂代谢及胰岛素抵抗的相关性

目的 探讨妊娠糖尿病（GDM）患者血清Betatrophin、脂肪组织中网膜素１（Omentin-1）水平变化特点,分析其与糖脂代谢和胰岛素抵抗的相关性。方法 选择2019年5月—2020年8月承德市中心医院妇产科接诊的179例孕妇。采用酶联免疫吸附试验检测所有孕妇妊娠10～14周血清Betatrophin、Omentin-1水平。所有孕妇妊娠24～26周进行口服葡萄糖糖耐量试验（OGTT）,其中,119例被诊断为GDM（GDM组）,60例血糖水平正常的孕妇为对照组。检测两组孕妇的总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）、空腹血糖（FBG）、空腹胰岛素（FINS）及糖化血红蛋白（HbA1c）水平。采用稳态模型计算胰岛素抵抗指数（HOMA-IR）。分析Betatrophin、Omentin-1与GDM患者糖脂代谢指标的相关性。多元线性逐步回归分析GDM患者HOMA-IR的影响因素。结果 GDM组孕前体重指数（BMI）、FBG、FINS、HOMA-IR、HbA1c、TC、LDL-C、Betatrophin水平高于对照组（P <0.05）,Omentin-1水平低于对照组（P <0.05）。相关性分析结果显示GDM患者血清Betatrophin与TG（r_s =0464）、HOMA-IR（r =0.610）、LDL-C（r =0.512）呈正相关（均P <0.05）,Omentin-1水平与孕前BMI（r_s =-0.304）、LDL-C（r =-0.543）、FBG（r =-0.475）、FINS（r =-0.435）、HOMA-IR（r =-0.576）呈负相关（均P <0.05）。多元线性逐步回归分析结果显示TG、LDL-C、Betatrophin、Omentin-1是GDM患者HOMA-IR的影响因素（P <0.05）。结论 GDM患者血清Betatrophin水平升高,Omentin-1水平降低,Betatrophin、Omentin-1均与GDM患者糖脂代谢、胰岛素抵抗有关。     

常规牙周基础治疗对2型糖尿病伴牙周炎的疗效研究

目的 观察常规牙周基础疗法治疗2型糖尿病伴牙周炎的疗效,并分析患者血糖、血清生化指标的变化。方法 选取2017年3月—2019年6月武汉市中医医院接受干预治疗的114例2型糖尿病伴牙周炎患者作为研究对象,根据随机数字表法分为对照组和研究组,每组57例。对照组选用常规治疗,研究组在常规治疗基础上予以牙周基础治疗;比较两组患者治疗前、后牙周噬菌斑数（PLI）、牙周袋底至龈缘距离（PD）、探诊后出血百分数（BOP）、出血指数（BI）及牙周袋至釉牙骨质界距离（CAL）;观察两组患者治疗前、后空腹血糖（FBG）和糖化血红蛋白（HbAlc）水平的变化,并分析血清生化指标水平,如甘油三酯（TG）、血钙（Ca）等。结果 两组患者治疗前PLI、PD、BOP、CAL及BI指标水平差异无统计学意义（P >0.05）;两组患者治疗后PLI、PD、BOP、CAL及BI指标水平差异有统计学意义（P <0.05）;两组患者治疗前HbAlc、T-Bil、FBG、Ca、P、Ca/P、TG、Lipoprotein a及ApoAl指标水平差异无统计学意义（P >0.05）;两组患者治疗后HbAlc、Ca/P、血清P、TG、ApoA1、T-Bil水平差异有统计学意义（P <0.05）。结论 牙周基础治疗能够改善2型糖尿病伴牙周炎患者Ca/P、HbAlc、血脂代谢水平及牙周参数,临床可推广及应用。     

左归降糖舒心方对高脂饮食MKR小鼠糖脂代谢及炎症反应的影响

目的 探讨左归降糖舒心方对高脂饮食转基因2型糖尿病MKR小鼠糖脂代谢及炎症反应的影响。方法 用左归降糖舒心方干预治疗高脂饮食转基因2型糖尿病MKR小鼠,采用电化学法测定空腹血糖浓度,采用全自动生化分析仪检测血脂和炎症因子超敏C反应蛋白（hs-CRP）,采用ELISA法检测血清胰岛素与肿瘤坏死因子α（TNF-α）水平。结果 高脂饲料喂养的MKR小鼠空腹血糖高于MKR组,但无统计学意义（P＞0.05）;血清胰岛素、血脂（TC、LDL-C、HDL-C）、血清hs-CRP和TNF-α水平高于MKR组（P＜0.01）,而三酰甘油（TG）下降,低于MKR组（P＜0.01）。经左归降糖舒心方或文迪雅干预治疗后,除左归降糖舒心方低剂量组血糖和hs-CRP下降不显著（P＞0.05）,其他各组MKR小鼠血糖、血清胰岛素、TG、血清hs-CRP和TNF-α水平显著下降（P＜0.05、0.01）,TC和LDL-C下降无统计学意义（P＞0.05）;左归降糖舒心方高剂量组HDL-C显著上升（P＜0.01）,而文迪雅组升高不显著（P＞0.05）。左归降糖舒心方作用呈剂量依赖性。结论 左归降糖舒心方能调节高脂饮食MKR小鼠糖脂代谢、减少炎症因子的产生、保护血管、降低糖尿病并发血管病变的风险。     

耳迷走神经电刺激治疗多囊卵巢综合征患者负性情绪的临床观察

[目的] 观察耳迷走神经电刺激治疗多囊卵巢综合征（PCOS）患者负性情绪的有效性。[方法] 采用随机数字表法将60例PCOS患者随机分为电刺激组和对照组，每组30例。两组患者均于月经第5天接受生活方式干预，电刺激组同时联合使用耳迷走神经电刺激治疗，两组治疗均以1个月为1个疗程，共治疗3个疗程。比较两组患者治疗前后的焦虑自评量表（SAS）评分、抑郁自评量表（SDS）评分、中医症状评分、体质量指数（BMI）、糖代谢指标[空腹胰岛素（FINS）、空腹血糖（FBG），计算稳态模型胰岛素抵抗指数（HOMA-IR）]及炎症因子[血清白细胞介素（IL）-1、IL-6、肿瘤坏死因子-α（TNF-α）]水平。[结果] 与治疗前比较，治疗后电刺激组患者SAS、SDS评分降低，中医症状评分降低，FINS、FBG、HOMA-IR降低，血清IL-1、IL-6和TNF-α水平降低（P<0.05）；对照组中医症状评分降低，FINS、FBG、HOMA-IR降低（P<0.05）。与对照组比较，治疗后电刺激组SAS、SDS评分降低，中医症状评分降低，FINS、FBG、HOMA-IR降低，血清IL-1、IL-6和TNF-α水平降低（P<0.05）。[结论] 在PCOS患者治疗中应用耳迷走神经电刺激，能够有效改善患者焦虑、抑郁状态，还可以改善患者血糖代谢，减轻炎症反应。     

糖尿病合并脑梗死患者血清HMGB1 水平及其意义

目的 观察糖尿病合并脑梗死患者血清高迁移率族蛋白B1（HMGB1）及其他血清指标水平,探讨血清HMGB1 与其他血清指标的关系。方法 选择该院糖尿病合并脑梗死患者60 例作为糖尿病合并脑梗死组,糖尿病患者60 例作为糖尿病组,健康体检者60 例作为对照组,收集3 组患者的临床资料、血清HMGB1 及其他血清指标水平。结果 糖尿病合并脑梗死组和糖尿病组患者血清HMGB1、高敏C 反应蛋白（hs-CRP）、空腹血糖（FBG）、糖化血红蛋白（HbA1c）、空腹胰岛素（FINS）、稳态胰岛素评价指数（HOMA-IR）、三酰甘油（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）水平高于对照组（P <0.05）,血清高密度脂蛋白胆固醇（HDL-C）水平低于对照组（P <0.05）,糖尿病合并脑梗死组患者血清HMGB1、hs-CRP、FBG、HbA1c、FINS、HOMA-IR、TG、TC、LDL-C 水平高于糖尿病组（P <0.05）,血清HDL-C 水平低于糖尿病组（P <0.05）。糖尿病合并脑梗死患者血清HMGB1 与血清hs-CRP、FBG、HbA1c、FINS、HOMA-IR、TG、TC、LDL-C 水平呈正相关（P <0.05）,与血清HDL-C 水平呈负相关（P <0.05）。多因素分析结果显示,FINS、HOMA-IR、TG、TC、LDL-C 是血清HMGB1 的影响因素（P <0.05）。结论 糖尿病合并脑梗死患者血清HMGB1 水平升高,血清HMGB1 与hs-CRP、血糖、血脂指标有关,FINS、HOMA-IR、TG、TC、LDL-C 是血清HMGB1 的影响因素。     

个体化减重干预对肥胖合并T2DM患者体质量指数、糖脂代谢的影响

目的 探讨个体化减重干预对肥胖合并2型糖尿病（T2DM）患者体质量指数（BMI）、糖脂代谢的影响。方法 选取2019年1月—2019年12月连云港第一人民医院收治的90例肥胖合并T2DM患者,随机分为对照组和观察组,每组45例。均予以常规降糖药物治疗,对照组同时予以基础饮食、运动干预,观察组同时予以个体化减重干预,均持续3个月。比较两组利拉鲁肽用药剂量;检测并比较治疗前、治疗1个月后、治疗2个月后、治疗3个月后的BMI、糖脂代谢指标;比较两组治疗期间不良事件发生率。结果 两组利拉鲁肽用药剂量比较,差异无统计学意义（P >0.05）;观察组与对照组治疗前、治疗1个月后、治疗2个月后、治疗3个月后的BMI、空腹血糖（FBG）、餐后2小时血糖（2 hPG）、糖化血红蛋白（HbA1c）、胰岛素抵抗指数（HOMA-IR）、胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）比较,采用重复测量设计的方差分析,结果 ①不同时间点的BMI、FBG、2 hPG、HbA1c、HOMA-IR、TC、TG、LDL-C、HDL-C有差异（P <0.05）;②两组的BMI、FBG、2 hPG、HbA1c、HOMA-IR、TC、TG、LDL-C、HDL-C有差异（P <0.05）,观察组BMI、FBG、2 hPG、HbA1c、HOMA-IR、TC、TG、LDL-C均低于对照组,观察组HDL-C高于对照组;③两组的BMI、FBG、2 hPG、HbA1c、HOMA-IR、TC、TG、LDL-C、HDL-C变化趋势有差异（P <0.05）。两组治疗期间不良事件发生率比较,差异无统计学意义（P >0.05）。结论 个体化减重干预可降低肥胖合并T2DM患者的BMI,调节糖脂代谢,且不良事件不会显著增多,相较于常规干预作用更大。     

肥胖儿童血清microRNA-27a、PPAR-γ与胰岛素抵抗的相关性研究

目的 探讨肥胖儿童血清microRNA-27a（miR-27a）、过氧化物酶体增殖物激活受体γ（PPAR-γ）与胰岛素抵抗（IR）的相关性。方法 选取2020年1月—2021年7月唐山市人民医院104例肥胖儿童为肥胖组，另选取同期该院体检的63名健康儿童为对照组。比较两组儿童体质量指数（BMI）、空腹血糖（FPG）、空腹胰岛素（FINS）、稳态模型评估（HOMA）-IR、总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、脂联素、瘦素、抵抗素、内脂素、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、miR-27a mRNA、PPAR-γ mRNA。Pearson或Spearman法分析肥胖儿童血清miR-27a mRNA、PPAR-γ mRNA与BMI、代谢指标、细胞因子水平的相关性。结果 与对照组比较，肥胖组BMI、FINS、HOMA-IR、TC、TG、LDL-C、瘦素、抵抗素、内脂素、IL-6、TNF-α、miR-27a mRNA升高（P <0.05），HDL-C、脂联素、PPAR-γ mRNA降低（P <0.05）。Pearson或Spearman法相关性分析结果显示，肥胖儿童血清miR-27a mRNA与BMI、FINS、HOMA-IR、TC、TG、LDL-C、瘦素、抵抗素、内脂素、IL-6、TNF-α呈正相关（r /r_s =0.607、0.362、0.466、0.435、0.542、0.450、0.546、0.558、0.437、0.633和0.559，均P <0.05），与HDL-C、脂联素、PPAR-γ mRNA呈负相关（r /r_s =-0.686、-0.607和-0.727，均P <0.05）；肥胖儿童血清PPAR-γ mRNA与BMI、FINS、HOMA-IR、TC、TG、LDL-C、瘦素、抵抗素、内脂素、IL-6、TNF-α呈负相关（r =-0.513、-0.328、-0.437、-0.340、-0.434、-0.411、-0.537、-0.514、-0.428、-0.547和-0.509，均P <0.05），与HDL-C、脂联素呈正相关（r =0.599和0.527，P <0.05）；肥胖儿童HOMA-IR与TC、TG、LDL-C、瘦素、抵抗素、内脂素、IL-6、TNF-α呈正相关（r /r_s =0.167、0.168、0.259、0.442、0.426、0.372、0.438和0.441，均P <0.05），与HDL-C、脂联素呈负相关（r =-0.240和-0.378，均P <0.05）。结论 肥胖儿童血清miR-27a mRNA高表达、PPAR-γ mRNA低表达，两者与IR密切相关。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.