共查询到17条相似文献,搜索用时 78 毫秒
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目的克隆表达矮秆蓖麻(Ricinus communis L)天冬氨酸蛋白酶(aspartic protease,AP)基因(RcAP)并进行蛋白纯化。方法 Q-PCR法验证高秆及矮秆蓖麻六叶期叶片RcAP基因表达量;提取矮秆蓖麻六叶期叶片总RNA,PCR扩增RcAP基因,克隆至pETM30载体中,构建重组表达质粒pETM30-RcAP[含谷胱甘肽-S-转移酶(glutathione-Stransferase,GST)标签],转化至E.coli DH5α感受态细胞中,优化IPTG诱导浓度及温度,SDS-PAGE分析目的蛋白的可溶性;采用亲和层析纯化重组蛋白GST-RcAP。结果矮秆蓖麻RcAP基因表达量为高秆蓖麻RcAP基因的3.7倍;经PCR及双酶切鉴定,重组表达质粒pETM30-RcAP构建正确;最适诱导条件为0.1 mmol/L IPTG 16℃诱导;重组蛋白GST-RcAP以可溶形式表达,相对分子质量约为62 000;纯化的重组蛋白GST-RcAP浓度为6 mg/mL。结论成功构建了重组表达质粒pETM30-RcAP,获得了可溶性重组蛋白GST-RcAP,本研究为解析RcAP蛋白... 相似文献
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蓖麻具有较高的经济价值。利用茎秆可压制建筑材料 ,它的种子可以榨油 :饼粕可以作肥料 ,脱毒处理后作饲料 ;叶可以养蓖麻蚕 ,发展绢纺业 ;从蓖麻籽提取的蓖麻油广泛用于航空和精密仪器的高级润滑油、刹车油和防护油。通过多年的研究 ,从蓖麻油中可以提炼出衍生物几百种用途不同的精细化工产品 ,其效益十分可观。因此 ,世界上各国十分重视蓖麻开发生产 ,加工及其产品综合利用。随着我国工业现代化进程的推进 ,利用蓖麻油及其衍生物的行业不断增加。内蒙古通辽化工厂以蓖麻籽为原料生产的精炼蓖麻油、脱水蓖麻油、氢化蓖麻油、1 2 -羟基硬脂… 相似文献
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Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
Linna Liu Hongwei Gao Jiping Li Ying Dong Ning Liu Jiayu Wan Wensen Liu Yucheng Sun Ming Xu 《International journal of molecular sciences》2009,10(5):2431-2439
The present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on intestinal brush border membranes (BBM) based on SPR technology in an attempt to mimic the real intestinal surface capable of interacting physically and/or actively with certain biological molecules. Combined with HPCE-ESI-MS indentification, we obtained 28 kinds of proteins in BBM that interacted with ricin. 相似文献
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甲壳素研究进展与应用 总被引:3,自引:0,他引:3
杜予民 《精细与专用化学品》2000,(14)
甲壳素是地球上的第二大再生资源,具有生理适应性、可完全分解性、多功能反应性、立体结构与手性、可再生性与亲水性。近年来,甲壳素在生物医用和环境友好功能材料方面取得了一些进展;由甲壳素经化学降解和酶解而生成的甲壳低聚糖也广泛应用在医药品、农用化学品、保健食品及化妆品中;甲壳素降解后生成的氨基葡萄糖也应用于生化试剂及医药中间体的合成。其他包括许多品种的甲壳素衍生物均在工业、纺织、食品行业和环保方面获得广泛应用。 相似文献
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David L. Brandon Thomas A. McKeon Stephanie A. Patfield Qiulian Kong Xiaohua He 《Journal of the American Oil Chemists' Society》2016,93(3):359-363
To facilitate the analysis of castor (Ricinus communis L.) seed fractions and germplasm for ricin content, we investigated the use of enzyme‐linked immunosorbent assay (ELISA) methods to differentiate between ricin toxin and the related Ricinus communis agglutinin (RCA). Both proteins are based on a heterodimeric AB structure, with a common A chain. RCA comprises two dimers of A and B chains. Both proteins are found in the meal remaining after castor oil extraction and impede the commercial production of castor seed in the USA. We identified pairs of monoclonal antibodies (mAbs) that could distinguish between the structurally related proteins that share a common A chain. Antibody specificity was determined by ELISA and checked by immunoblotting. We found that mAb–mAb pairs afforded quantification of each castor protein, and that a mAb paired with a commercial polyclonal antibody provided detection of both with comparable sensitivity. 相似文献
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查阅近几年地骨皮相关文献,对其进行分类、整理、归纳并分析,对地骨皮的药理及临床应用研究进展进行了综述。结果表明:地骨皮具有降血糖、降血压血脂、抑菌抗病毒、解热镇痛、增强免疫等药理活性,其临床应用广泛,可用于治疗功能性低热、糖尿病、牙痛、高血压、疮疡、慢性荨麻疹、过敏性紫癜、外科术后持续发热等病症,但对地骨皮的药理及临床应用仍有待深入研究。 相似文献
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野菊花活性成分及应用的研究进展 总被引:4,自引:0,他引:4
野菊花主要成分有黄酮类、萜类、挥发油、野菊花内酯、胡萝卜甙、氨基酸及微量元素等多种物质,具有扩张冠状动脉、降血压、降血糖、预防高血脂、广谱抗菌、解热、保护心脏、抑制血小板凝集、增强免疫、抗炎、抗病毒、清除氧自由基、抗肿瘤、抗衰老、保护视力等多种活性。就野菊花的化学成分、生物活性、药理价值及主要成分的提取方法进行综述。 相似文献