磷酸化大豆分离蛋白质功能特性的研究

本文采用三氯氧磷（POCl3）对大豆分离蛋白（SPI）进行磷酸化改性，并研究了磷酸化前后SPI功能特性的变化。结果表明：磷酸化SPI的溶解性、乳化性以及粘度等功能特性都有不同程度的改变。     

海鱼鱼鳔资源利用研究

采用三氯氧磷（POCl3）对大豆分离蛋白（SPI）进行磷酸化改性,并研究了磷酸化前后SPI功能特性的变化.结果表明：磷酸化SPI的溶解性、乳化性以及粘度等功能特性都有不同程度的改变.     

聚磷酸钠对大豆分离蛋白的修饰研究

采用多聚磷酸钠对大豆分离蛋白进行磷酸化修饰,研究磷酸化反应的工艺条件及改性后大豆分离蛋白几种功能特性的变化。结果表明:当大豆分离蛋白4%、三聚磷酸钠9%、反应初始pH9、反应时间3h时,磷酸化程度最大;磷酸化后的大豆分离蛋白的溶解性、乳化性以及粘度都有不同程度的改善。     

大豆分离蛋白磷酸化改性研究

采用低摩尔比的三氯氧磷(POCl3)/蛋白质对大豆分离蛋白(SPI)进行磷酸化改性.利用响应面法确定了最佳改性工艺条件,并且研究了最佳工艺条件下改性后SPI功能特性的变化.结果表明,最佳改性工艺条件为:SPI浓度4%,反应时间30min,POCl3体积0.20mL,pH10.00.磷酸化SPI等电点由4.25降低至3.75,溶解性和乳化能力有明显提高.     

磷酸化大豆蛋白功能特性的研究

采用三聚磷酸钠(STP)对大豆分离蛋白进行化学改性,得出3％大豆分离蛋白磷酸化程度最大的工艺条件为pH8.0,STP浓度为3％,35℃下保温3.5小时,此时的磷酸化程度为57％。测定了改性前后,不同改性程度下大豆分离蛋白的水溶性、乳化能力、发泡能力、持水能力以及流变特性等的变化。结果表明改性后大豆蛋白的等电点由pH4.5漂移到3.9,与此同时,大豆蛋白的功能特性也有了很大的改善。用红外光谱,证实了STP与大豆蛋白反应的实质就是赖氨酸残基进行氨基磷酸酯化反应。     

大豆蛋白去酰胺改性的研究

研究了磷酸盐对大豆分离蛋白进行去酰胺改性，在既保持大豆蛋白的大分子构象，又提高大豆分离蛋白功能性质的条件下，得出去酰胺改性的最佳工艺条件为：磷酸盐浓度为０．２６２ｍｏｌ，ｐＨ８．１４，在１０２℃下反应３ｈ。测定了改性前后大豆分离蛋白水溶性，乳化能力，发泡能力，持水性以及粘度等的变化。结果表明改性后大豆蛋白的等电点随去酰胺程度的增加，向低ｐＨ值移动，大豆蛋白的功能性质均有不同程度的提高和改善。     

微波辅助磷酸化改性提高大豆分离蛋白乳化性的研究

以提高大豆分离蛋白乳化性为目的,微波辅助磷酸化改性大豆分离蛋白,测定改性前后大豆分离蛋白乳化性,并与磷酸化改性方法进行比较.结果显示,相较空白对照、微波对照和磷酸化对照组,微波辅助磷酸化蛋白粉乳化性显著增加,其乳化活性分别提高了3、1.9和1.3倍,乳化稳定性提高了1.3、1.2、1.1倍,乳化性在考察范围内随磷酸化程度的增加而增大(P＜0.05).微波辅助磷酸化改性技术参数为预处理2 min,微波功率600 W,微波时间3 min.相较磷酸化改性方法,将改性时间由180 min缩减至3 min,大幅提高效率、节约成本,具有广阔的应用前景.     

大豆蛋白去酰胺改性的研究

本文研究了磷酸盐对大豆分离蛋白（ＳＰＩ）进行去酰胺改性，在既保持大豆蛋白的大分子构象，又提高大豆分离蛋白功能性质的条件下，得出去酰胺改性的最佳工艺条件为：磷酸盐浓度为０．２６２Ｍ，ｐＨ８．１４，在１０２℃下反应３ｈ。测定了改性前后大豆分离蛋白水溶性，乳化能力，发泡能力，持水性以及粘度等的变化。结果表明改性后大豆蛋白的等电点随去酰胺程度的增加，向低ｐＨ值移动，大豆蛋白的功能性质均有不同程度的提高和改善。并用红外光谱（ＦＴ－ＩＲ）和差示扫描量热法（ＤＳＣ）对改性前后大豆分离蛋白结构的变化进行了研究。     

大豆分离蛋白的磷酸化改性研究

采用三聚磷酸钠 (STMP)对大豆分离蛋白 (SPI)进行磷酸化改性。研究了不同改性程度下SPI功能特性的变化。结果表明 :磷酸化SPI等电点由 pH 4 4 1移至pH 3 86,溶解性、乳化能力和持水性也有明显提高     

微波辅助糖基化对大豆分离蛋白乳化性的影响

该文采用微波辅助糖基化改性大豆分离蛋白以提高大豆分离蛋白(soybean protein isolate,SPI)的乳化性。通过单因素实验研究微波时间、大豆分离蛋白与葫芦巴胶质量比、糖基化反应时间、反应温度对改性大豆分离蛋白乳化性的影响,并运用响应面法优化微波辅助糖基化改性大豆分离蛋白的最佳工艺条件,研究结果显示,在微波时间3 min、SPI与葫芦巴胶质量比1∶3、反应时间41 min、反应温度58℃时,改性大豆分离蛋白乳化性达到最高,糖基化程度达到最佳的水平,与只进行微波改性的大豆分离蛋白相比,乳化活性提高了51.33%,乳化稳定性提高了294.14%;与未改性的大豆分离蛋白相比,乳化活性提高了88.67%,乳化稳定性提高了788.84%。利用红外光谱和紫外光谱表征改性产物,结果表明大豆分离蛋白与葫芦巴胶发生了糖基化反应。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press     

INFOPRINT 2014 was Successfully Held in Dongguan

Sponsored by Printing and Printing Equipment Industries Association of China （PEIAC） and organized by Print China magazine, the Seventeenth Beijing International Printing Information Conference （INFOPRINT 2014） was successfully held on 11th Dec. 2014 at Dongguan Exhibition International Hotel.