沼泽红假单胞菌高产辅酶Q10菌株的诱变选育研究

以沼泽红假单胞菌为出发菌株,通过紫外诱变和罗红霉素抗性筛选,以期获得辅酶Q10高产菌株.试验结果表明,紫外照射时间60-70s时可能达到最大正向突变概率.通过紫外诱变、罗红霉素初筛和发酵复筛,获得一株沼泽红假单胞菌突变株R-1,辅酶Q10产量达到0.021g/L,较出发菌株产量（0.018g/L）提高16％.经过6次传代培养,突变菌株产辅酶Q10产量稳定,可用于进一步研究.     

产生物表面活性剂石油降解菌筛选及特性研究

目的获得产高效生物表面活性剂的菌株,并判定表面活性剂的结构及探索其特性。方法通过从富油土壤中采用富集培养、血平板分离、排油活性等方法筛选高产表面活性剂菌株并鉴定;采用萃取和柱层析法提纯后HPLC-MS法分析产物结构并分析其理化性质。结果筛选出产生物表面活性剂高效菌BD-5,经鉴定为铜绿假单胞菌;所产生物表面活性剂为8种鼠李糖脂同系物的混合物;鼠李糖脂溶液对液体石蜡、柴油和甲苯都具有较强的乳化能力;当鼠李糖脂浓度高于临界胶束浓度(CMC)时,长链烷烃和多环芳烃在水相中的表观溶解度随鼠李糖脂浓度的增大而增大,摩尔增溶比(MSR)的变化关系为正十六烷>萘>菲>芘。结论 BD-5菌株产生的生物表面活性剂活性突出,有良好的应用前景。     

鼠李糖脂对两株木质素降解菌产酶能力的影响

研究了生物表面活性剂鼠李糖脂对黄孢原毛平革菌、简青霉产木质素降解酶能力的影响．结果表明,鼠李糖脂对酶活性的影响与鼠李糖脂的浓度、投加形式、菌种及酶的种类有关．0．0065％的鼠李糖脂不同程度地促进了黄孢原毛平革菌产LiP,MnP,简青霉产LiP,漆酶,0．013％的鼠李糖脂可以促进黄孢产LiP,简青霉产漆酶,但却明显抑制了另外两种酶的活性;鼠李糖脂可以使简青霉产MnP的高峰期提前,但不能提高酶活性．通过影响产酶能力,鼠李糖脂使两株菌对木质素的降解率均高于对照样．此外,接种鼠李糖脂产生菌——铜绿假单胞菌的效果达到甚至超过添加鼠李糖脂的促进效果．     

污染环境中假单胞菌产鼠李糖脂的初步研究

从受重油和焦化废水污染的水及土壤中分离出产鼠李糖脂的假单胞菌（Ｐｓｅｕｄｏｍｏｎａｓ）１２株,发现其菌群分布、糖脂产量与特定污染环境有一定相关性,产糖脂量较高的假单胞菌群是该污染环境中的优势降解菌．     

甲胺磷降解菌的生长特性及其降解活性的研究

从农药厂污水中分离到一株能以甲胺磷为唯一碳源、氮源和能源生长的铜绿假单胞菌,在充分供氧条件下,研究了该菌株降解甲胺磷的降解过程,生长条件以及影响因素,结果表明：当甲胺磷浓度为0.5g/L时,培养5d降解率达79.6%,该菌甲胺磷的最佳生长浓度为0.5g/L,最适pH为7.0,温度为30℃,对甲胺磷的最高耐受浓度为30g/L,12种金属离子对该菌的生长和甲胺磷的降解有不同程度的抑制作用,加入较低浓度的碳氮源,能促进该菌对甲胺磷的降解。     

新疆罗布泊周边胀果甘草内生芽孢杆菌BLG-542产碱性纤维素酶最佳条件的研究

文章以罗布泊胀果甘草中分离出的78个菌株中初步筛选出7株产碱性纤维素酶的菌株为研究对象,进行产碱性纤维素酶最佳优化条件的研究.碱性的胀果甘草内生芽孢杆菌菌株接种于CMC-Na为碳源,复合蛋白胨为氮源的培养基中,分析研究菌株产碱性纤维素酶的最佳条件优化.通过从78株本实验室于2006年保藏的罗布泊胀果甘草内生菌中筛选出能产生胞外碱性纤维素酶的7株菌,其中酶活力最高的菌株为BLG-542.本研究主要以酶活力最高的菌株BLG-542为出发菌株进行研究.经过对产酶条件优化,获得了胀果甘草内生菌产碱性纤维素酶最佳配方培养基:D-木糖10g/L,复合蛋白胨10g/L,可溶性淀粉10g/L,酵母粉5g/L,K2 HPO4 1g/L,MgSO4 0.2g/L,NaCl 20g/L,Na2CO3 10g/L;实验产酶最佳接种量为6％,最佳培养温度为37℃,最佳培养时间为48h,产酶最佳PH为9.0;菌种生长最佳PH为7.6,经过优化产酶条件纤维素酶活力从优化前的3.8U/mL提高到6.26 U/mL,是优化之前的1.8倍;该研究表明特殊生态环境的植物内生菌与次生代谢产物、活性物质、酶类有密切的关系,环境除对内生菌酶的产生极其活性影响外,还影响着内生菌的次生代谢.该地区植物内生菌均有潜在的应用价值.     

RL在液态培养条件及堆肥介质中的生物降解研究

为了研究生物表面活性剂的环境友好性,考察其应用于堆肥中是否会时堆肥产生二次污染,本实验自制了生物表面活性剂鼠李糖脂,考察其在液态培养条件及堆肥介质中的生物降解情况．实验结果表明：不同的微生物对鼠李糖脂有不同的降解效果,其源生菌铜绿假单胞菌没法降解鼠李糖脂,但鼠李糖脂对微生物都是无毒的．在堆肥基质中的降解表明其可以被降解,但不是优先降解物质,可见鼠李糖脂可在堆肥中起一定作用后被降解。     

氧化葡萄糖酸杆菌的选育及其发酵条件优化

目的 提高氧化葡萄糖酸杆菌的生物量.方法 采用紫外诱变育种、梯度平板半理性设计筛选、单因素实验进行培养条件优化、2 L发酵罐内进行补料培养.结果 筛选到了一株生物量高产菌株,命名为Gouv2007,其生物量比原始菌株提高了11.2%,脱氢酶比酶活与原始菌株持平,培养时间缩短了6 h.该菌生长的最佳碳源为山梨醇,氮源为酵母粉,无机离子为硫酸镬和磷酸氢二钾;最佳培养条件为:培养温度28℃,500 mL烧瓶装液量100 mL、摇床转速200 r/min、培养基的初始pH自然.在最佳条件下培养,其生物量为1.34 g/L,比原始菌株提高了50.6%,脱氢酶比酶活有所提高.在2 L发酵罐内补料培养,其生物量达到了5.58 g/L.结论 通过紫外诱变育种和条件优化提高了该菌的生物量,并在发酵罐内进行了小试.     

甲胺磷降解菌的紫外诱变及高产菌株的筛选

通过紫外诱变育种从假单胞菌S-2中筛选出一株S-232。试验表明S-232在30℃,摇床220r/min的条件下6天降解甲胺磷达到74%,比出发菌株降解率提高了8~9%。30代降解结果表明S-232降解情况相对稳定。     

生物表面活性剂鼠李糖脂性质的研究

为探究鼠李糖脂用作生物表面活性剂的潜力,以铜绿假单胞菌1.10452发酵生产的鼠李糖脂为研究对象,利用高效液相色谱-质谱联用法(HPLC-MS)进行成分鉴定,并测定其临界胶束浓度(CMC)、乳化性、起泡性及抗菌活性.成分分析结果表明,该鼠李糖脂主要由9种同系物组成,且以双鼠李糖脂为主要成分.鼠李糖脂的临界胶束浓度为80...     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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