Chromosome behavior of heat shock induced triploid in Fenneropenaeus chinensis

Triploidy was induced in Chinese shrimpFenneropenaeus chinensis by 30±0.5°C heat shock treatment (initiated at 20 min after fertilization) for 10 min to inhibit the release of PB2 at 18.0°C. The highest triploid rate obtained was 84.5% in nauplius stage. The effect of heat shock treatment on meiosis and cleavage of eggs was investigated in this work aimed to establish efficient procedures for triploid induction and to gain understanding of the mechanism of triploid production. Three pronuclei that could be observed in the treated eggs under fluorescence microscope developed into triploid embryos. Some abnormal chromosome behavior was observed in heat shocked eggs. Contribution No. 4407 from the Institute of Oceanology, Chinese Academy of Sciences. This work was supported by Project under Major State Basic Development Program (G1999012009) and Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX 2-211)     

Effects of temperature on the respiratory metabolism,feeding and expression of three heat shock protein genes in Anadara broughtonii

Anadara broughtonii is one of the main commercially important species of shellfish in northern China.A.broughtonii lives in relatively stable subtidal zone where the clam could re spond to environmental changes with minimum energy.Therefore,slight fluctuations in water environment may have a great impact on physiological processes such as feeding and metabolism.Large-scale mortality often occurs during the intermediate rearing processes,and high temperatures in summer are considered the leading cause of mortality.To understand the physiological and molecular response patterns of A.broughtonii at high temperatures and to estimate the appropriate metabolism temperature for A.broughtonii,the effects of temperature on the respiratory metabolism and food intake at different growth stages were studied.The response patterns of heat shock protein genes to heat stress and post-stress recovery were also explored.Results show that the temperature and growth stage of A.broughtonii were two important factors affecting metabolism and feeding.The optimum temperature for feeding and physiological activities in each shelllength group was 24℃.The temperature at which the peak metabolic rate occurred in each shell-length group increased with the increase in shell length.With the increase in heat stress,the expression of three heat shock protein genes(Abhsp60,Abhsp70,and Abhsp90) in the tissues of two size groups of A.broughtonii increased significantly when temperature was above 24℃ and reached a peak at 30℃.After heat shock at30℃ for 2 h,the expression of the three heat shock protein genes rapidly increased,peaked at 2 h after the heat shock,and then gradually decreased to the level of the control group at 48 h after the heat shock.The three heat shock protein genes respond rapidly to heat stress and can be used as indicators to the cellular stre ss response in A.broughtonii under an environmental stre ss.     

Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature

High temperature influences the homeostasis of fish.We investigated the effects of elevated temperature on tissues of Japanese flounder {Paralichthys olivaceus) by analyzing the histology and heat shock protein 70(hsp70) expression of fish reared in warm conditions.In this study,temperature was increased at l±0.5℃/day starting at 24±0.5℃,and was kept at that temperature for 5 days before the next rise.After raising temperature at the rate up to 32±0.5℃,tissue samples from midgut,spleen,stomach,liver,muscle,gill,heart,trunk kidney and brain were collected for histological analysis and mRNA assay.Almost all the tissues showed changes in morphological structure and hsp70 level at 32±0.5℃.Histological assessment of the tissues indicated that the gill had the most serious damage,including highly severe epithelial lifting and edema,curved tips and hyperemia at the ending of the lamellars,desquamation and necrosis.The next most severe damage was found in liver and kidney.The hsp70 levels in all the tissues first increased and then decreased.The gut,stomach,muscle,heart,and brain had the highest expressions in 6 h,whereas the spleen,liver,gill and kidney had the highest expressions in 2 h.Therefore,tissues with the most significant lesions(especially gill and liver) responded much earlier(2 h) in hsp70 expression than other tissues,and these tissues demonstrated the most marked histological disruption and elevated mRNA levels,making them ideal candidates for further studies on the thermal physiology of this species.     

Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations

Small heat shock proteins encompass a widespread but diverse class of proteins,which play key roles in protecting organisms from various stressors.In the present study,the full-length cDNAs of two small heat shock proteins(MgsHSP22 and MgsHSP24.1)were cloned from Mytilus galloprovincialis,which encoded peptides of 181 and 247 amino acids,respectively.Both MgsHSP22 and MgsHSP24.1 were detected in all tissues examined by real-time PCR,with the highest expression being observed in muscle and gonad tissues.The real-time PCR results revealed that Cd signifi cantly inhibited MgsHSP22 expression at 24 h and MgsHSP24.1 at 24 and 48 h under 5 μg/L Cd 2 + exposure.MgsHSP24.1 expression was also signifi cantly inhibited after 50 μg/L Cd 2+ exposure for 48 h.With regard to antioxidant enzymes,increased GPx and CAT activity were detected under Cd 2+ stress(5 and 50 μg/L),while no signifi cant difference in SOD activity was observed throughout the experiment.Overall,both MgsHsps and antioxidant enzymes revealed their potential as Cd stress biomarkers in M.galloprovincialis.     

Acclimation-dependent expression of heat shock protein 70 in Pacific abalone (Haliotis discus hannai Ino) and its acute response to thermal exposure

Heat shock protein 70 (Hsp70) is one important member of heat shock protein (Hsp) family that is responsible for various stresses, especially thermal stress. Here we examined the response of Hsp70 gene to both chronic and acute thermal exposure in Pacific abalone (Haliotis discus hannai Ino). For the chronic exposure, abalones were maintained at 8, 12, 20, and 30°C for four months and their mRNA levels were measured. The highest mRNA level of Hsp70 gene relative to actin gene was detected in the 30°C-acclimated group, followed by the 8°C-acclimated group and then the 12°C- and 20°C-acclimated groups. After the long-term acclimation, gills from each of the above acclimation groups were dissected and exposed to different temperatures between 8°C and 38°C for 30 min. Hsp70 expression in gills acclimated to different temperatures responded differentially to the same temperature exposure. The incubation temperature that induced maximum Hsp70 mRNA expression was higher in the higher temperature acclimation groups than lower temperature groups. Pacific abalones could alter the expression pattern of Hsp70 gene according to environmental thermal conditions, through which they deal with the stress of thermal variations.     

Genome-Wide Identification of heat shock protein 10/60 Genes in Japanese Flounder(Paralichthys olivaceus) and Their Regulated Expression After Bacterial Infection

Heat shock proteins 10/60(hsp10/60) are a family of conserved ubiquitously expressed heat shock proteins which are produced by cells in response to exposure to stressful conditions. Besides the chaperone and housekeeping functions, they are also known to be involved in immune response during bacterial infection. In this study, we identified and annotated 10 hsp10/60 genes through bioinformatic analysis in Japanese flounder(Paralichthys olivaceus). Among them one member of hsp10(hspe) family and nine members of hsp60(hspd) family were identified. Phylogenetic and selection pressure analysis showed that the hsp10/60 genes were evolutionarily constrained and their function was conserved. Besides, hsp10/60 genes were involved in different embryonic and larval stages and acted as the sentinel role in an unchallenged organism. In addition, we also observed the expression patterns of hsp10/60 genes after Edwardsiella tarda infection, for the first time in Japanese flounder. Eight out of 10 genes were differentially expressed after bacterial challenges, the significantly regulated expressions of flounder hsp10/60 genes after bacterial infections suggested their involvement in immune response in flounder. Our results provide valuable information for clarifying the evolutionary relationship, and early insights of the immune functions of hsp10/60 genes in Japanese flounder.     

Salicylic acid and heat acclimation pretreatment protects Laminaria japonica sporophyte (Phaeophyceae) from heat stress

Possible mediatory roles of heat acclimation and salicylic acid in protecting the sporophyte of marine macroalga Laminaria japonica (Phaeophyceae) from heat stress were studied. Heat stress resulted in oxidative injury in the kelp blades. Under heat stress significant accumulation of hydrogen peroxide (H₂O₂) and malonaldehyde (MDA), a membrane lipid peroxidation product, and a drastic decrease in chlorophyll a content were recorded. Activity of the enzymatic antioxidant system was drastically affected by heat stress. The activity of superoxide dismutase (SOD) was significantly increased while peroxidase (POD), catalase (CAT) and glutathione peroxidase (GPX) were greatly inhibited and, simultaneously, phenylalanine ammonia-lyase was activated while polyphenol oxidase (PPO) was inhibited. Both heat acclimation pretreatment and exogenous application of salicylic acid alleviated oxidative damage in kelp blades. Blades receiving heat acclimation pretreatment and exogenous salicylic acid prior to heat stress exhibited a reduced increase in H₂O₂ and MDA content, and a lower reduction in chlorophyll a content. Pretreatment with heat acclimation and salicylic acid elevated activities of SOD, POD, CAT, GPX and PPO. Considering these results collectively, we speculate that the inhibition of antioxidant enzymes is a possible cause of the heat-stress-induced oxidative stress in L. japonica, and enhanced thermotolerance may be associated, at least in part, with the elevated activity of the enzymatic antioxidant system.     

Simulation and analysis for anthropogenic heat release

Heat balance of urban ecosystem is a key point for the study of urban climate and micro-climate pattern and its change mechanism. Urban heat island effect is becoming increasingly serious,which is mainly caused by the change of the earth's surface cover and the anthropogenic heat release. In this study,the simulation experiment for the anthropogenic heat release was designed according to the heat balance principle. A set of buildings of miniature city were used to constitute the residential area,U grooves were applied to simulate the single building,and the fluorescent lamps in the U groove were regarded as the heat sources of the anthropogenic heat release. The simulation experiment was launched with long-short wave sun photometer,sonic anemothermometer and heat flow gauge in the experiment site. Then the net solar radiation,sensible heat flux and heat flux into the ground were determined. The quantities of the anthropogenic heat release were calculated based on the heat balance principle,and were compared with the theoretical power consumption of the fluorescent lamps. The root mean square error( RMSE) of the simulation for the anthropogenic heat release reaches0. 078 W·m- 2,a comparatively high precision,which showes that the anthropogenic heat release can be accurately determined through the simulation experiments. This study provided a scientific method for the purpose of monitoring the anthropogenic heat release.     

Triploidy induction in Pacific oysterCrassostrea gigas by caffeine with thermal shock

Zygotes of Pacific oyster (Crassostrea gigas) were treated for triploid induction with caffeine (10 mmol/L, 15mmol/L and 20mmol/L) in combination with thermal shocks (at 40 minute post-fertilization) lasting for 5 and 10 minutes. The highest yield of triploids, 41.5%, was obtained from the treatment with 20 mmol/L caffeine at 34°C lasting 10 minutes. The triploid levels were less than 30.0% in other treatments. Triploid induction was more effective in treatment with 15–20 mmol/L caffeine at 34–38 °C than with lower concentrations of caffeine at temperatures below 32 °C. Our results suggest that triploid induction with caffeine in combination with thermal shocks is less efficient than some other methods reported previously.     

Contribution of oceanic circulation to the polewar heat flux

Oceanic contribution to the poleward heat flux in the climate system includes two components: the sensible heat flux and the latent heat flux. Although the latent heat flux has been classified as atmospheric heat flux exclusively, it is argued that oceanic control over this component of poleward heat flux should play a critically important role. The so-called swamp ocean model practice is analyzed in detail, and the critical role of oceanic circulation in the establishment of the meridional moisture transport is emphasized.     

青藏高原大气热源气候特征的研究

用NCEP/NCAR再分析资料和小波分析方法分析研究了1950-2005年青藏高原大气热源气候特征和变化特征,主要结论包括:(1)夏季青藏高原东部大气热源的强度明显较西部大.6月份,高原东部热源的强度是高原西部的近两倍,7月份的值也比西部大了40%以上.(2)青藏高原全区、东部和西部逐年平均的大气热源有明显不同的变化特征.高原全区年平均大气热源的变化主要是一个14年的时间尺度;高原东部不仅有14年的主要时间变化尺度,同时还有一个非常显著的2.6年的时间变化尺度;高原西部则不同,是一个不明显的1-2年的时间尺度.     

Research on ground heat exchanger of Ground Source Heat Pump technique

Ground Source Heat Pump technique and its operating principle are described in this paper. Ground heat exchanger is the key technique of ground source heat pump and its pattems are discussed. Software is helpful to design ground heat exchanger. A project of Chinese Ground Source Heat Pump is introduced and its market is more and more extensive.     

A probe heat flow value of the East China Sea shelf

Bottom temperature variation (BTV) is a serious problem in determining the thermal gradient and heart flow of the sediments in shallow seas. The water depth of the East China Sea shelf is mostly below 150m, and the heat flow measurement is strongly affected by BTV. Following a statistical algorithm, we rechecked the temperature and thermal conductivity data of the cruises KX90-1 and KX91-1, carried out by a cooperation program of China and Japan, and calculate the heat flow in a site without long-term temperature record. The calculated heat flow in the site was 58.6±3.6 mW/m², being just within the range of the drill heat flow value of East China Sea shelf. The inversed amplitude spectrum of BTV has a peak in frequency of 1/10 per year, and the annual component is also an important part. Comparison with two lakes of Lake Greifensee and Lac Leman (i.e. Lake Geneva), which are in different water depth, revealed that with increasing water depth, the peak of amplitude spectrum moved towards low frequency components. The heat flow values calculated in this paper and from petroleum bore hole in East China Sea shelf are much more close to that in southeast China than in Okinawa Trough. Supported by the National High Technology R&D Program of China (2004 AA616060)     

Research on ground heat exchanger of Ground Source Heat Pump technique

Ground Source Heat Pump technique and its operating principle are described in this paper. Ground heat exchanger is the key technique of ground source heat pump and its pattems are discussed. Software is helpful to design ground heat exchanger. A project of Chinese Ground Source Heat Pump is introduced and its market is more and more extensive.     

冬季塑料大棚供暖系统的自动控制

介绍以MCS－8051单片机为核心，与其它相关部件组成的一智能化供暖系统。用于冬季塑料大棚蔬菜育苗和栽种，实现温度的自动测量和自动控制，可将塑料大棚内的温度始终控制在适合蔬菜生长的温度范围内。     

The seasonal variation of the North Pacific Meridional Overturning Circulation heat transport

Based on the 50-year Simple Ocean Data Assimilation(SODA) reanalysis data, we investigated the basic characteristics and seasonal changes of the meridional heat transport carried by the North Pacific Meridional Overturning Circulation. And we also examined the dynamical and thermodynamic mechanisms responsible for these heat transport variability at the seasonal time scale. Among four cells, the tropical cell(TC) is strongest with a northward heat transport(NHT) of(1.75±0.30) PW(1 PW=1.0×10 15 W) and a southward heat transport(SHT) of(-1.69±0.55) PW, the subtropical cell(STC) is second with a NHT of(0.71±0.65) PW and SHT of(-0.63±0.53) PW, the deep tropical cell(DTC) is third with a NHT of(0.18±0.03) PW and SHT of(-0.18±0.11) PW, while the subpolar cell(SPC) is weakest with a NHT of(0.09±0.05) PW and SHT of(-0.07±0.09) PW. These four cells all have dif ferent seasonal changes in their NHT and SHT. Of all, the TC has stronger change in its SHT than in its NHT, so do both the DTC and SPC,but the seasonal change in the STC SHT is weaker than that in its NHT. Therefore, their dynamical and thermodynamic mechanisms are dif ferent each other. The local zonal wind stress and net surface heat flux are mainly responsible for the seasonal changes in the TC and STC NHTs and SPC SHT, while the local thermocline circulations and sea temperature are primarily responsible for the seasonal changes of the TC,STC and DTC SHTs and SPC NHT.     

An experiment on induction of triploidy inEriocheir japonicus hepuensis Dai by heat treatment

Temperature treatment to inhibit extrusion of the polar body of the egg was first used on a lower crustacean,Artemia salina (Gross, 1932), and then was used for inducing triploids of amphibian (Frankhauser and Griffiths, 1939), fish (Svardson, 1945), and mammal (Beatly and Fishchberg, 1949). So far, induction of triploidy has been extensively used to obtain sterile or quick-growth individuals in fish (Swarup, 1956; Lincoln, and Scott, 1983 and Thorgaard, 1986) and mollusk (Stanley et al., 1984), but similar work has not been reported on crab, a higher crustancean.     

Evaluation of suitability area by ground source heat pump system in Baotou plain

According to the elements of hydrogeological conditions, lithology, stratigraphic thermal physical pa- rameters, construction technology and economic rational elements, the evaluation system of ground source heat pump （GSHP） were established in Baotou plain. The factor weights were determined by using analytic hierarchy process, and the comprehensive element method was selected to evaluate the region suitability of GSHP system. The results show that the suitable area is 559.63 km^2 for utilizing GSHP in the studied area, accounting for 62.58% of the total area; while the unsuitable area is 334.60 km2, accounting for 37.42%. The evaluating results can provide not only a scientific basis for development and utilization rationally of shallow geothermal heat in Baotou, but also offer an important reference to the other areas.