Con-A诱发的淋巴细胞化学发光

本文以刀豆素 A(ConA)为诱发剂,研究了绵羊肺淋巴液中的淋巴细胞鲁米诺依赖的化学发光现象.结果表明,ConA诱发的淋巴细胞化学发光测定具有迅速、简便和敏感等优点,可作为淋巴细胞活化的检测方法.淋巴细胞悬液(106～107个细胞/ml)1ml、ConA溶液(2mg/ml)0.1ml、鲁米诺溶液(10-3M)50μl为最佳测定体系.多形核白细胞(PMN)混杂可明显增强发光体系的发光强度.因此,测定外周血中的淋巴细胞化学发光,细胞的纯化是一个不可忽视的问题.消炎痛可抑制其发光,说明发光可能与花生四烯酸代谢有关.     

鲁米诺-H2O2流动注射化学发光法测定乐果

在碱性介质中,乐果能够有效增强鲁米诺-H2O2体系的化学发光,据此建立了测定乐果的流动注射化学发光分析方法,并对反应的机理进行了探讨.在最佳条件下,乐果-鲁米诺-H2O2体系化学发光强度在2s内达到了最大值,乐果在5.0×10-8-1.0×10-5 g/mL范围内呈良好的线性关系,检出限(S/N=3)为1.5×10-8 g/mL.该体系应用于加标蔬菜样品测定,乐果测定回收率为108.0%-119.3%,测定偏差为2.7%-4.6%.化学发光的机理可能是由于乐果先被过氧化氢氧化生成过氧化磷酸盐,过氧化磷酸盐氧化鲁米诺生成激发态,从而产生发光.     

影响鲁米诺体系电致化学发光因素的研究

系统研究了鲁米诺体系的电致化学发光行为 ,发现电学参数和溶液的酸碱性很大程度地影响电化学发光的产生、光强及连续性。确定了在碱性环境中的最佳电化学发光条件 ,发现在 KOH- KCl( p H=12 .5)介质中 ,于 Pt电极上施加 +1.3V( vs.Ag/ Ag Cl)的正矩形脉冲 ,可得到鲁米诺的最佳发光信号。发光强度与鲁米诺的浓度在 1.0× 10 -7— 1.0× 10 -5mol/ L范围内呈线性关系。并讨论了 H2 O2 及硫脲对鲁米诺的电致化学发光的影响。     

过氧化氢—次氯酸钠氧化鲁米诺发光的研究

过氧化氢－次氯酸钢可氧化碱性鲁米诺而产生强化学发光，对影响发光的诸因素及化学发光机理进行了研究，并探讨了该化学发光反应在过氧化氢测定中的应用。     

三唑磷-鲁米诺-H2O2发光体系的研究与应用

研究了碱性介质中三唑磷-鲁米诺-H2O2体系的化学发光特征,建立了三唑磷流动注射化学发光分析方法,并对反应机理进行了探讨.研究了三唑磷-鲁米诺-H2O2体系的化学发光反应动力学,考察了pH、流速、鲁米诺-H2O2配比等因素对化学发光的影响.在最佳条件下,过氧化氢HOO-负离子可能与三唑磷的磷原子反应生成磷的过氧化合自由基,与鲁米诺作用使得体系化学发光得到增强,3s内达到了最大值;应用于三唑磷的测定,在1.0×10-8-1.0×10-5g/mL范围内呈良好的线性关系,检出限(S/N=3)为6.7×10-9g/mL;测定蔬菜样品,三唑磷浓度分别为1.0-5.0μg/mL时,测定回收率为82.3%-108.6%,测定偏差为1.6%-4.7%.     

鲁米诺增强声致发光应用研究的进展与问题

本文结合自己的工作，介绍了化学发光剂鲁米诺增强声致发光应用研究中的几种典型情况，并相应地 一些得到探讨的问题。     

流动注射-化学发光法用于药物分析研究进展

简述了近年来国内外流动注射-化学发光法在药物分析领域中的主要运用。主要介绍了鲁米诺-H2O2体系、鲁米诺-铁氰化钾体系、鲁米诺-高碘酸盐体系、鲁米诺-KMnO4体系、鲁米诺-Ag(Ⅲ)配合物体系以及其他体系在药物分析中的运用。     

溶解氧对鲁米诺电化学发光的增强效应研究

本文对溶解氧增强鲁米诺的电化学发光(ECL)行为进行了系统的研究,发现电学参数等因素很大程度地影响溶解氧增强ECL的产生、发光强度及稳定性。通过对电极的预处理,在0.15 mol·L-1的KNO3-中性硼酸缓冲溶液中,-0.4-0.9 V(vs.Ag)的矩形脉冲激励下,溶解氧显著增强鲁米诺的ECL且具有良好的稳定性,线性范围从0-16 mg·L-1,相对标准偏差为0.4％。讨论了溶解氧增强鲁米诺ECL的机理及表面活性剂的影响。     

化学发光法快速检测海洛因

酯酶为脱乙酰剂 ,促使海洛因分子水解生成吗啡。在 5 0 μmol/ L鲁米诺 ,2 .4 μmol/ L H2 O2 及 0 .4 mol/ LNa2 CO3 (p H9— 11)条件下 ,吗啡 -鲁米诺 -过氧化体系具有较高的发光强度。 EDTA作为掩蔽剂可对部分阳离子的干扰予以掩蔽 ,化学发光法快速检测海洛因 ,该法检测速度快、灵敏度高 ,检出限为3× 10 -9mol/ L。     

N-溴代丁二酰亚胺氧化体系流动注射化学发光法测定水中苯酚

在碱性介质中,N-溴代丁二酰亚胺氧化鲁米诺产生化学发光,苯酚对反应具有很强的抑制作用,据此建立了N-溴代丁二酰亚胺-鲁米诺-苯酚化学发光体系测定水中苯酚的新方法。当苯酚的浓度在1.0×10-5～9.0×10-4 mg·mL-1范围内,其浓度与发光强度呈良好的线性关系, 检出限是1.81×10-7 mg·mL-1, 相对标准偏差为1.11%, 将该方法直接用到水样中苯酚的测定, 取得满意的结果。并探讨了该化学发光反应的机理。     

Electrogenerated chemiluminescence of luminol at a carbon nanotube-perfluorosulfonate polymer (Nafion) modified gold electrode

A multi-wall carbon nanotube/Nafion modified gold electrode (CNT/Nafion/GE) was fabricated by casting the composite film on the electrode surface. Electrogenerated chemiluminescence (ECL) of luminol at the modified gold electrode was studied under conventional cyclic voltammetry in alkaline Na₂CO₃-NaHCO₃ buffer solution. Three ECL peaks were obtained. The most strong ECL peak (ECL-1) was enhanced about 20-fold at the CNT/Nafion modified gold electrode compared with that at the bare gold electrode. The emitter of all the ECL peaks was indentified as 3-aminophthalate. The intensities of ECL peaks were found to depend on the ratio of CNT/Nafion, the electrolytes, the pH, and the presence of O₂ and N₂. The mechanisms of all ECL peaks have been proposed. The results indicate that carbon nanotubes have a significantly catalytic effect on luminol ECL, which might further expand the analytical application of nanomaterial-modified electrode in the field of electrogenerated chemiluminescence.     

过氧化氢－次氯酸钠氧化鲁米诺发光的研究

过氧化氢－次氯酸钠可氧化碱性鲁米诺而产生强比学发光，对影响发光的诸因素及化学发光机理进行了研究，并探讨了该化学发光反应在过氧化氢测定中的应用     

二元酚类化合物的流动注射化学发光抑制分析法

发现邻苯二酚,对频丁基邻苯地酚,对苯二酚,３,４－二羟基苯甲对Ｃｏ＾２＋催化的鲁米诺－过氧化化学发光反应有较强的抑制作用,在此基础上建立了四种二元酚类化合物的流动 射化学发光抑制分析法。     

A Sensitive Chemiluminescence Flow Injection Procedure for Assay of Fluoride in Waters and Humane Urine by Use of Immobilized Reagents

A new simple, rapid, selective and sensitive analytical procedure based on chemiluminescence (CL) detection is described for the determination of free fluoride at sub‐nanogram levels by use of controlled‐reagent‐release technology in a flow injection system. The analytical reagents involved in the CL reaction, including luminol and periodate, were both immobilized on anion‐exchange resins in a flow injection system. Through water injection luminol and periodate were eluted from the anion exchange column to generate the chemiluminescence, which was enhanced in the presence of fluoride. The increased CL intensity was linear with fluoride concentration in the range from 0.1 to 10 ng·mL^? 1. The limit of detection was 20 pg·mL^? 1 (3σ) and the relative standard deviation (RSD) was 1.02% (n = 5) for a 1.0 ng·mL^? 1 fluoride. At a flow rate of 2.0 mL·min^? 1, including sampling and washing, a typical analytical procedure could be performed in 0.5 min with a RSD of less than 3.0%. The proposed method was successfully applied to determine the free fluoride in water and human urine, and the results were in good agreement with those obtained by ion chromatography.     

分子印迹流动注射化学发光法测定盐酸强力霉素

研究发现在碱性条件下,盐酸强力霉素对鲁米诺-铁氰化钾体系化学发光反应具有明显的增敏作用,据此建立了分子印迹-流动注射化学发光法定量分析盐酸强力霉素的新方法。以甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂合成了盐酸强力霉素的分子印迹聚合物。以此分子印迹聚合物为分子识别物质,利用盐酸强力霉素-鲁米诺-铁氰化钾化学发光体系,结合流动注射化学发光分析技术,建立了测定盐酸强力霉素高选择性的分子印迹-流动注射化学发光分析方法。方法的线性范围为9.0×10-7～6.0×10-5 g·mL-1,检出限为3.2×10-7 g·mL-1。对6.0×10-6 g·mL-1的盐酸强力霉素水溶液进行分析,9次平行测定的相对标准偏差为3.5%。利用此方法测定尿样和盐酸强力霉素药片中盐酸强力霉素的含量,结果令人满意。     

Sensitive Chemiluminescence Assay for Patulin in Apple Juice by Flow Injection

Abstract

A sensitive chemiluminescence (CL) procedure for the determination of patulin by flow injection is described. It was found that patulin inhibits the CL generated from the luminol–dissolved oxygen system significantly. The decrement of chemiluminescence intensity was linear with the patulin concentration over the range from 0.04 to 10.0 ng mL^?1 with a detection limit of 0.01 ng mL^?1 (3σ_noise). At a flow rate of 2.0 mL min^?1, a complete analytical process could be performed within 0.5 min, including sampling and washing, with a relative standard deviation of less than 3.0% (n=5). The proposed method was applied successfully in the determination of patulin in apple juice, and the recovery was between 96.9% and 103.9%.     

反相流动注射-化学发光法测定盐酸多巴胺

基于在碱性介质中,盐酸多巴胺对鲁米诺-铁氰化钾化学发光反应体系的强烈抑制作用,建立了反相流动注射抑制化学发光测定盐酸多巴胺的新方法。研究了影响化学发光强度的因素,并探讨了化学发光反应的可能机理。该方法快速、准确、线性范围宽,测定盐酸多巴胺的检出限为1.14×10-9 g·mL-1,方法的线性范围为2.0×10-9～8.0×10-7 g·mL-1,对于4.0×10-7 g·mL-1盐酸多巴胺测定11次的相对标准偏差为0.99%。应用于盐酸多巴胺注射液中盐酸多巴胺的测定,结果满意。     

Ultrasound-pretreatment combined with Ti3C2-TiO2-AuNPs enhancing the electrogenerated chemiluminescence of the air-saturated luminol for exosomes detection

It is still a great challenge to develop effective strategies to improve the low electrogenerated chemiluminescence (ECL) of air-saturated luminol. Herein, the synergistic effects of Ti₃C₂-TiO₂-AuNPs nano hybrid and high-intensity focused ultrasound pretreatment (ultrasound-pretreatment) were used to significantly improve the ECL emission of the air-saturated luminol, and the mechanism was proposed. The ultrasound-pretreatment as a green method with the cavitation effect could form O₂^–• and H₂O₂ in situ as an initiator. TiO₂ and Au nanoparticles (AuNPs) were in situ decorated on the Ti₃C₂ surface to form Ti₃C₂-TiO₂-AuNPs, and it was proved as a highly efficient booster which could catalyze and aggregate H₂O₂ to the O₂^–•. The utilization rate of intermediates has been greatly improved. Exosomes as model targets can be sensitively detected by the ECL sensor. The detection limit was 195 particles μL⁻¹. The detection results of exosomes in actual samples are satisfactory. We believe that the ultrasound-pretreatment strategy could be extended to the sensitive detection in the biological sample.     

Enhancement effect of CdTe quantum dots-IgG bioconjugates on chemiluminescence of luminol-H2O2 system

In this paper we developed an entirely new and highly sensitive luminol-H₂O₂ flow injection chemiluminescence system using the enhancement effect of CdTe quantum dots-IgG bioconjugates. Immunoglobulin G (IgG) as a kind of bio-molecule was conjugated to different sized CdTe semiconductor quantum dots (QDs). Using PL spectra and CL intensity profiles, it was found that chemiluminescence resonance energy transfer (CRET) was possibly occurring between CdTe-IgG bioconjugate and luminol. Under optimum conditions, increase of IgG concentration in CdTe-IgG bioconjugate resulted enhancing effect on CL intensity of luminol-H₂O₂ system. Moreover quenching effects on CL intensity by addition of different proteases can construct turn off biosensor for these proteases with low detection limits and wide linear range. Furthermore, the effects of various organic and inorganic species on CdTe-IgG bioconjugates enhanced luminol-H₂O₂ CL system were also studied in this paper.