共查询到20条相似文献,搜索用时 46 毫秒
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An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition 总被引:1,自引:0,他引:1
Gregory PA Bracken CP Smith E Bert AG Wright JA Roslan S Morris M Wyatt L Farshid G Lim YY Lindeman GJ Shannon MF Drew PA Khew-Goodall Y Goodall GJ 《Molecular biology of the cell》2011,22(10):1686-1698
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YoonSeok Choi Hoe Suk Kim Jisu Woo Eun Hye Hwang Kyoung-Won Cho Soonhag Kim Woo Kyung Moon 《PloS one》2014,9(7)
The epithelial-mesenchymal transition (EMT) plays important roles in tumor progression to metastasis. Thus, the development of an imaging probe that can monitor transient periods of the EMT process in live cells is required for a better understanding of metastatic process. Inspired by the fact that the mRNA expression levels of zinc finger E-box-binding homeobox 1 (ZEB1) increase when cells adopt mesenchyme characteristics and that microRNA-200a (miR-200a) can bind to ZEB1 mRNA, we conjugated molecular beacon (MB) mimicking mature miR-200a to magnetic nanoparticles (miR-200a-MB-MNPs) and devised an imaging method to observe transitional changes in the cells during EMT. Transforming growth factor-β1 treated epithelial cells and breast cancer cell lines representing both epithelial and mesenchymal phenotypes were used for the validation of miR-200a-MB-MNPs as an EMT imaging probe. The real-time imaging of live cells acquired with the induction of EMT revealed an increase in fluorescence signals by miR-200a-MB-MNPs, cell morphology alterations, and the loss of cell-cell adhesion. Our results suggest that miR-200a-MB-MNPs can be used as an imaging probe for the real-time monitoring of the EMT process in live cells. 相似文献
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Xiao Ling Li Toshifumi Hara Youngeun Choi Murugan Subramanian Princy Francis Sven Bilke Robert L. Walker Marbin Pineda Yuelin Zhu Yuan Yang Ji Luo Lalage M. Wakefield Thomas Brabletz Ben Ho Park Sudha Sharma Dipanjan Chowdhury Paul S. Meltzer Ashish Lal 《Molecular and cellular biology》2014,34(3):533-550
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Xiaowei Wang Yong Xu Ying-Chun Zhu Ya-Kun Wang Ji Li Xiao-Ying Li Tingting Ji Shou-Jun Bai 《Journal of cellular physiology》2019,234(8):12926-12933
Diabetic nephropathy (DN) is a kind of microvascular complications of diabetes. Long noncoding RNAs (lnRNAs) can participate in the development of various diseases, including DN. However, the function of lncRNA NEAT1 is unclear. In our present study, we reported that NEAT1 was significantly increased in streptozotocin-induced DN rat models and high-glucose-induced mice mesangial cells. We observed that knockdown of NEAT1 greatly inhibited renal injury of DN rats. Meanwhile, downregulation of NEAT1-modulated extracellular matrix (ECM) proteins (ASK1, fibronectin, and TGF-β1) expression and epithelial–mesenchymal transition (EMT) proteins (E-cadherin and N-cadherin) in vitro. Previously, miR-27b-3p has been reported to be involved in diabetes. Here, miR-27b-3p was decreased in DN rats and high-glucose-induced mice mesangial cells. The direct correlation between NEAT1 and miR-27b-3p was validated using the dual-luciferase reporter assay and RNA immunoprecipitation experiments. In addition, zinc finger E-box binding homeobox 1 (ZEB1), which has been identified in the process of EMT clearly contributes to EMT progression. ZEB1 was predicted as a target of miR-27b-3p and overexpression of miR-27b-3p dramatically repressed ZEB1 expression. Therefore, our data implied the potential role of NEAT1 in the fibrogenesis and EMT in DN via targeting miR-27b-3p and ZEB1. 相似文献