视黄酸及干扰素对胃癌细胞系MKN45中p16,p21,c-myc基因表达的影响

目的探讨全反式视黄酸及干扰素两种因子对胃癌MKN45细胞的影响.方法将视黄酸及干扰素同时加入胃癌细胞系MKN45中进行细胞培养,用MTT法测定细胞的生长状况,并通过Northern blot和免疫组化测定p16,p21及c-myc的表达情况.结果干扰素(IFN)协同全反式视黄酸(ATRA)可有效地抑制MKN45细胞生长,癌细胞经联合用药诱导后p16和p21基因的表达水平提高,c-myc基因表达水平下降.视黄酸受体RARα基因在MKN45细胞中呈低水平表达,经ATRA和IFN诱导后表达水平提高.结论 ATRA联合IFN诱导可调节p16和p21基因的表达水平,抑制胃癌MKN45细胞生长,这可能与细胞中RARα基因高水平表达有一定关系.     

TNF与IFN协同诱导癌细胞凋亡机制的研究进展

综述了肿瘤坏死因子(TNF)与干扰素(IFN)协同诱导肿瘤细胞凋亡的机制.分别从受体、细胞周期、相关基因及凋亡途径4个方面进行阐述,以期为细胞因子的游离及固定药物的机制研究提供理论参考,并对肿瘤细胞因子治疗的临床用药有指导意义.     

γ干扰素的研究与临床应用

γ干扰素 (IFN -γ)是干扰素家族中比较特殊的一类 ,除了有抗病毒作用以外 ,它还是人体内一种双向免疫调解因子 ,在抗肿瘤 ,治疗自身免疫性疾病方面有广阔的应用前景。     

线粒体外膜通道蛋白VDAC与靶向肿瘤细胞凋亡

VDAC(Voltage-dependent anion channel)是位于线粒体外膜上的一种主要通道蛋白,参与线粒体内外物质和能量的运输,在线粒体与细胞其它部位的通讯中起重要调节作用.近年来研究发现,VDAC也是线粒体与其它蛋白质相互作用的功能结合位点,可与多种凋亡调节蛋白(如HK-Ⅰ/Ⅱ、Bcl-2家族蛋白、tubulin、MAP2/4等)以及非蛋白调节因子相互作用,参与调控细胞凋亡.因此,VDAC成为线粒体凋亡通路中一种关键的靶蛋白.本文对近年来VDAC在肿瘤细胞凋亡中的作用机制进行简要综述.     

干扰素-γ对母胎界面免疫调控的影响

人类和其他哺乳动物的妊娠过程是自然界中存在的一种同种半异体移植免疫耐受模型，近来的研究发现母胎界面上表达的主要组织相容性复合体（MHC）分子有重要作用，经典的和非经典的MHC I/II类抗原表达在妊娠过程中是时空动态变化的，维持妊娠的正常进行.生理剂量的干扰素-γ（IFNγ）是妊娠必需的细胞因子之一，而超过生理剂量的IFNγ则会直接导致流产.IFNγ影响妊娠的机制是多方面的，主要通过调节母胎界面MHC抗原的表达.与此同时，IFNγ下调在妊娠过程中发挥多种生物学功能的细胞因子IL-1β和TGFβ1的表达；促进母胎界面细胞凋亡水平；抑制孕酮的分泌.超生理剂量IFNγ可通过这些作用最终导致母胎界面局部免疫微环境调控失衡，妊娠失败.文中着重介绍近年来母胎界面免疫调控及IFNγ对其作用机制的研究进展.     

分化抑制基因Id4的沉默对MEL细胞的影响及相关基因表达分析

将反义Id4导入MEL细胞株,研究此基因对细胞增殖、分化及凋亡的影响.结果表明,Id4基因的沉默显著抑制细胞增殖,细胞的分化率明显提高(84倍);细胞的死亡率和凋亡率均增加,但基因沉默对分化的促进占优势,Id4可能是调控和保持分化的重要因子.反义Id4对细胞周期引擎基因、肿瘤抑制基因、凋亡抑制基因以及着丝粒蛋白基因表达的影响,说明Id基因的功能可以从细胞周期检验点调控等方面加以探讨.这些结果有利于细胞增殖、分化调节,特别是血细胞分化控制的研究.     

细胞凋亡对衰老的调节在肿瘤的发生发展及治疗的研究进展

细胞凋亡是细胞死亡的一种形式,是调节机体正常发育的重要机制,涉及一系列在维持细胞自稳中发挥重要作用的调控因子.肿瘤的发生、发展与治疗也受到凋亡调控蛋白的调控,细胞凋亡也参与多种与衰老相关的病理过程,本研究就细胞凋亡通路及其对衰老及肿瘤的相关调控机制进行综述.     

转录调节因子DEC1的研究进展

DEC1（Differentiated embryo-chondrocyte expressed gene1）是一个碱性螺旋一环一螺旋（bHLH）结构的转录因子,在软骨形成、神经发生、免疫应答、分子钟的调控、细胞分化、肿瘤的发生中起着重要的作用。DEC1还是一个缺氧调节基因,与肿瘤细胞在缺氧环境下存活密切相关,可调控肿瘤细胞生长、凋亡相关因子如缺氧诱导因子1α、转化生长因子-β、信号转导和转录激活因子、P53等的表达。DEC1还可通过调节同一家族的分子如DEC2的表达来调节细胞的功能。本文就其研究进展加以概述。     

生物活性化合物ML-098的首次合成

正恶性肿瘤的生长过程主要表现为癌细胞的产生、发展、增殖以及侵袭.细胞凋亡是机体重要的生理过程,对维持体内环境稳定起着重要作用.恶性肿瘤的产生和发展与细胞凋亡与增殖的调节失控密切相关,在多步骤肿瘤发生的早期,细胞凋亡异常将导致细胞生长周期延长,促进了变异基因的累积,有助于肿瘤的发生.Rho GTPases家族是公认的调节细胞运动的因子,其主要是通过影响     

LRH-1在女性生殖和乳腺癌中的研究进展

肝受体类似物-1 (liver receptor homolog-1, LRH-1;NR5A2)是转录因子家族中的新成员,表达于肝、肠、胰腺和卵巢,尤其在卵巢高表达,参与胚胎发育的调节.近年来发现LRH-1还表达于人类乳腺中未分化脂肪组织的间质层,可能参与前脂细胞功能调节或脂肪细胞分化.因此对LRH-1的研究有助于揭示LRH-1与人类生殖及疾病的关系,为疾病的早期诊断和有效治疗提供了新靶点.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

正~~