共查询到19条相似文献,搜索用时 57 毫秒
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引言制备或大型液相色谱因在温和条件下具有高分辨分离能力且操作简单而广泛应用于药物、生物化学品和碳氢化合物等的分离和高纯化.其应用的范围和规模正在不断发展.然而,传统的吸附色谱因其吸附剂利用率低,生产效率低.为克服这一缺陷,Wankat,Barker和Ganetsos以及Ruthven和Ching等人比较了各种逆流、错流和模拟移动床色谱分离技术和原理,提出各种柱组合分离的方法.模拟移动床色谱能获得较高生产效率,但其设备和操作较复杂,一定程度上限制了它的应用.循环色谱是一种新颖的分离技术,这一概念最早是由Bombaugh和Biesenberger… 相似文献
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双柱循环色谱分离过程的最优化 总被引:2,自引:0,他引:2
提出一种双柱循环色谱分离过程最优化方法。以分离产率作为目标函数 ,以回收率作为约束条件 ,应用参数灵敏度分析法比较各参数对分离产率影响的大小 ,选择灵敏度大的操作参数作为可优化参数 ,采用改进单纯形法对双柱循环色谱分离蔗糖还原糖过程进行了最优化计算。参数灵敏度分析结果表明 ,吸附剂填充高度和进料负荷具有较高的灵敏度 ,是影响分离产率的主要因素 ,被选为可优化参数。与平面搜索法比较 ,使用文中提出的优化方法不仅能获得与使用平面搜索法一致的结果 ,而且计算简便、收敛速度较快 ,文中提出的最优化方法双柱循环色谱分离过程进行最优化是可行的和有效的 相似文献
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卢建刚 《中国化学工程学报》2003,11(2):234-239
A non-linear non-ideal model,taking into account non-linear competitive isotherms,axial disperison,film mass transfer,intraparticle diffusion,and port periodic switching,was developed to simulate the dynamics of simulated moving bed chromatography(SMBC),The model equations were solved by a new efficient numerical technique of orthogonal collocation on finite elements with periodical movement of conceantration vector,The simulated SMBC performance is in accordance with the experimental results reported in the literature for separation of 1,1‘‘‘‘‘‘‘‘-bi-2-naphtol enantiomers using SMBC,This model is useful for design,operation ,optimization and scale-up of non-linear SMBC for chiral separations with significant non-ideal effects,especially for high solute concentration and small intraparticle diffusion coefficient or large chiral stationary phase particle. 相似文献
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钢液RH精炼非平衡脱碳过程数学模拟:过程数学模型 总被引:2,自引:2,他引:0
考虑和分析了钢液RH非平衡脱碳过程的特征,基于冶金反应工程学和非平衡态热力学及气-液两相流的双流体模型和修正的紊流k-ε模型,引入熵衡算和非线性耗散因子,对该过程提出了一个新的三维数学模型,给出了该模型的具体细节,包括控制方程的建立、修正的k-ε双方程模型、相应的源项和边界条件的确定等;对90 t装置内的RH和RH-KTB精炼过程,讨论和确定了该模型各有关参数. 相似文献
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模拟精馏过程的新方法──三维非平衡混合池模型应用 总被引:3,自引:0,他引:3
将三维非平衡混合池模型作了适当简化,并对烃类物系和非理想溶液进行了模拟计算,模拟结果与生产实测数据和文献报道值十分接近。 相似文献
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柱层析法分离姜油树脂中的姜酮 总被引:1,自引:0,他引:1
研究了柱层析分离姜油树脂中姜酮的实验条件,确定了洗脱剂成分,以及洗脱剂的最佳用量及流速.结果表明,使用不同配比的正己烷与乙醚混合溶液作洗脱剂,目标产物姜酮在φ(正己烷∶乙醚)=2∶3馏分段;洗脱液体积选择110 mL/0.8 g姜油树脂,流速选择1.5 mL·min-1时姜酮能被较好地分离. 相似文献
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高效液相色谱法分离纯化酯型儿茶素的研究 总被引:14,自引:0,他引:14
采用反相高效液相色谱技术 ,分离制备粗儿茶素粉末中表没食子儿茶素没食子酸酯( EGCG)、没食子儿茶素没食子酸酯 ( GCG)、表儿茶素没食子酸酯 ( ECG)三种酯型儿茶素馏分。制备色谱条件 :色谱柱 ,( 80 mm i.d× 5 0 0 mm,Fuji C18,粒径 2 5~ 40 μm) ;流动相 :有机醇 A- 1 /水( 1 6.5 /83.5 ,v/v,流速为 5 8m L/min;检测波长 2 78nm,进样量 30 0 0 mg。将所收集相应馏分分别经 P- 1大孔树脂吸附脱去溶剂 ,低沸点溶剂洗脱 ,旋转蒸发浓缩洗脱液 ,冷冻干燥后 ,得到 EGCG、GCG、ECG白色粉末。各物质经 HPLC检测纯度达 99.2 %以上。该法适用于分离纯化 EGCG、GCG、ECG三种酯型儿茶素单体 相似文献
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逆流色谱是一种不用固态支撑体或载体的液液分配色谱技术,其分离原理具有手性制备性分离的优势。以近年采用逆流色谱技术对手性化合物分离的应用进行了综述。 相似文献
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《分离科学与技术》2012,47(18):2907-2912
Mupirocin is an antibiotic from Pseudomonas fluorescens used topically against Staphylococci infections. There are many patented strategies which are proposed for separation of the antibiotic from the fermentation broth comprising of liquid-liquid extraction and hydrophobic chromatography. The paper demonstrates the use of native silica, as used in flash chromatography, as adsorbent for normal phase liquid chromatography of mupirocin. Mobile phase for elution was acidified n-hexane: ethyl acetate (10/90 v/v). The method achieved a yield of 66% relative to the mupirocin source and a concentration factor of 13. Anti-microbial assays, high performance liquid chromatography and Fourier transform infrared spectroscopy has been used to confirm the identity of the antibiotic after chromatographic separation. An alternative route to isolation of mupirocin using the polar sorbent has been suggested. 相似文献
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《分离科学与技术》2012,47(15):2065-2087
Abstract Mathematical equations have been formulated to predict the longitudinal concentration distribution in the case of instantaneous behavior of a continuous chromatographic system. Partial differential equations are initially obtained from mass balance on the differential element of column height. The formulated equations are solved by Lap lace transformation and the contour integration method. Two equations are obtained, one for the column section above the feed point and the second for the column section below the feed. Both of these equations give the concentration distribution along the appropriate section of the column. The model predicts the instantaneous and steady-state behavior of the continuous moving-bed chromatographic system. The model can also be used as a tool for the design of new continuous chromatographic columns. 相似文献
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The separation method using chiral stationary phase (CSP) for the preparation of enantioselective compound was widely used. In this work, supercritical fluid chromatography(SFC) was proposed to resolve the chiral mixtures. To determine the optimum operating conditions for the chiral separation of the racemic ibuprofen, the retention factors and resolutions with the change in pressure, temperature and the content of IPA (%, by volume) in supercritical CO2 were investigated. Experiments showed that the retention factor decreased with the increase of pressure and decrease in temperature. The retention factor was also influenced by the content of IPA in mobile phase, as the content of IPA in the supercritical fluid increased, the retention factor decreased. The resolution of the enantiomers became worse with the increase of IPA in the supercritical fluid. Through optimizing the experimental conditions, a SFC procedure with 13MPa, 311.15K and 4% IPA in CO2 was obtained. The peak shape of the enantiomers was symmetric with supercritical fluid chromatography when compared to the asymmetric peak shape obtained by the conventional liquid chromatography. This work demonstrated that the developed supercritical fluid chromatography procedure was suitable for the chiral separation of ibuprofen enantiomers. 相似文献
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离心分配色谱技术及其在天然产物分离中的应用 总被引:3,自引:0,他引:3
作为现代逆流色谱的一种,离心分配色谱(CPC)是一种新型的制备色谱技术。简单介绍了离心分配色谱的设备原理和操作方法,综述了离心分配色谱的研究现状和其应用于天然产物分离方面的相关报道,特别对离心分配色谱反应器和离子交换置换离心分配色谱等一些CPC研究领域出现的新技术进行了介绍;对离心分配色谱的研究前景进行了展望。 相似文献
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