拮抗芽孢杆菌对蒙古黄芪主要药效成分的影响

探讨黄芪根腐病拮抗芽孢杆菌 SXKF16-1(Bacillus atrophaeu)和 SXKF16-2(B.methylotrophicus)对黄芪主要药效成分的影响,旨在为后续多功能制剂的开发提供依据.采用灌根法对健康黄芪苗浇注拮抗菌发酵液,HPLC-UV-ELSD法对10种药效成分进行测定.结果表明,毛蕊异黄酮葡...     

膜荚黄芪C4H基因的克隆及表达分析

本研究采用RACE技术从膜荚黄芪中克隆得到两个肉桂酸-4-羟基化酶AmC4H1与AmC4H2,并进行了生物信息学分析;研究了AmC4H1与AmC4H2在根、茎和叶中的表达量与毛蕊异黄酮及其糖苷含量之间的相关性.结果表明,克隆得到的AmC4H1与AmC4H2基因均编码505个氨基酸,属于不稳定蛋白和亲水性蛋白,AmC4H...     

TRAIL诱导肿瘤细胞凋亡机制的研究进展

TRAIL具有选择性杀伤肿瘤细胞而对大多数正常细胞没有明显毒性,可通过TRAIL胞外及胞内凋亡通路发挥诱导肿瘤细胞凋亡作用,而正常细胞则通过NF-κB介导、诱骗受体及c-FLIP调节来逃逸TRAIL诱导的凋亡。TRAIL与基因治疗、抗死亡受体的单克隆抗体、化疗、放疗药物及蛋白酶体抑制因子的联合使用能明显提高肿瘤治疗的敏感性,同时还可以逆转肿瘤细胞对TRAIL的耐药现象。因此TRAIL作为新一代抗肿瘤药物有望应用于临床治疗。     

三氧化二砷诱导肿瘤细胞凋亡机制的研究进展

收集了近年来国内外关于三氧化二砷诱导肿瘤细胞凋亡作用的研究资料,综述了三氧化二砷在体外研究中的凋亡作用机制,并对其机制进行分析,以期为人类疾病的临床治疗和动物疾病的临床治疗提供更多的理论依据。     

Inducing Apoptosis Effects of Human sTRAIL on Hepatocellular Carcinoma Cells

To investigate the effects of human soluble TRAIL(sTRAIL) on cell growth and apoptosis of hepatocellular carcinoma cell line SMMC7721.After treatment of sTRAIL,the cell proliferation and apoptosis are determined by invertible microscope,trypan blue exclusion test,MTT assay,terminal deoxynucleotidly transferase mediated labeling(TUNEL)and DNA fragmentation assay.Chromatin condensation is observed by invertible microscope,Proliferation of SMMC7721 cell is significantly inhibited after the treatment with sTRAIL,TUNEL results show in comparison with control,the difference of the apoptosis index of cells treated by sTRAIL is significant,and DNA ladder is observed.The results indicate that sTRAIL can induce apoptosis of hepatocellular carcinoma cell line SMMC7721.     

Apoptosis of Hepatoma Cells HepG-2 Induced by Shikimic Acid from FRUCTUS ANISI STELLATI and Its Mechanism

[Objective]To research the apoptosis of hepatoma cells HepG-2 induced by shikimic acid from FRUCTUS ANISI STELLATI and its mechanism. [Methods]Hepatoma cells HepG-2 at logarithmic phase were randomly divided into drug group and control group. Shikimic acids from FRUCTUS ANISI STELLATI at the concentrations of 0. 125,0. 5 and 1 g /L were used. Inhibitory effects of shikimic acids on HepG-2 cell proliferation were detected by MTT method. Effects of shikimic acids at the concentrations of 0. 125,0. 5 and 1 g /L on the cell cycle of hepatoma cells HepG were researched by Flow Cytometer. Expression of Bcl-2 and Bax proteins were detected by immunocytochemistry detection. [Results]Results of MTT test showed that shikimic acid(0. 125,0. 5 and 1 g /L) had significant inhibitory effects on the proliferation of in vitro human liver cancer HepG-2 in a time-and dose-dependent relationship(P 0. 05). Shikimic acid blocked the hepatoma cells HepG-2 at G1 phase,so that the cells could not enter into S phase for DNA synthesis,which restricted the cell proliferation. Results of immunocytochemistry detection showed that protein expression of Bcl-2 decreased significantly,while Bax expression enhanced significantly,so that the proportion of Bcl-2 to Bax reduced. [Conclusions]shikimic acid blocked the hepatoma cells HepG-2 at G1 phase,and had significant inhibitory effects on the proliferation of human hepatoma cell through reducing the proportion of Bcl-2 to Bax.     

Apoptosis of CT26 Cells of Mouse Colon Cancer Induced by the Combination of Arsenic Trioxide and Paclitaxel and Its Mechanism

[Objective]To study the apoptosis of CT26 cells of mouse colon cancer induced by the vombination of arsenic trioxide(As2O3)and paclitaxel(PTX) and to research its mechanism. [Methods]CT26 cells of mouse colon cancer were treated by different concentrations of As2O3 for 72 h. Cell proliferation rate was detected by MTT method. Then,As2O3 having 30% inhibitory concentration was selected to carry out research together with different concentrations of PTX; cell proliferation rate was detected by MTT method. The dosage group having the most significant inhibitory effects was selected for the further research. Finally,apoptosis rate and cell cycle of each group were assessed by flow cytometer. [Results]Different concentrations of As2O3 or PTX significantly inhibited CT26 cell proliferation in a dose dependent manner.Combination of 2. 0 μmol /L As2O3 and 0. 04 μmol /L PTX had synergistic inhibition effects on CT26 cell proliferation,and showed more significant effects on enhancing the apoptosis rate of cells.[Conclusions] Combination treatment of As2O3 and PTX inhibited cell growth and induced apoptosis.     

山楂抗氧化活性成分及作用机制研究进展

氧化应激与某些疾病的发生发展密切相关,因此寻找有效的抗氧化物质并阐明其作用机制具有重要作用。山楂具有明显的抗氧化作用,其抗氧化活性主要与调节体内抗氧化酶活性和清除体内自由基有关。本文综述了近年来山楂抗氧化活性成分及其作用机制的研究进展,以期为山楂抗氧化应激活性的深入研究及其相关产品的开发应用提供依据。     

Inhibitory Effects of AURKB Gene on Apoptosis and Cancer Cell Growth in HCT 116 Cells

[Objectives]To explore the inhibitory effect of AURKB gene in apoptosis and cancer cell growth in HCT 116 cells.[Methods]The in vitro cytology studies were carr...     

Inhibitory Effects of Polyphenols from Pinus koraiensis Seed Scale on the Proliferation of Human Cancer Cells in Vitro

[Objective] To research on the inhibitory effects of polyphenols from Pinus koraiensis seed scale on the proliferation of human cancer cells in vitro. [Methods] Polyphenols from Pinus koraiensis seed scale were prepared into extracting solutions at different concentrations. Suspension cultures of five tumor cells were processed,which were lung carcinoma cell A549,human bone marrow neuroblastoma cell SH-SY5 Y,human skin cancer cell A375,human hepatocarcinoma cell HepG-2 and human ovarian cancer cell SKOV3. Inhibitory rate of cell proliferation in vitro was detected by MTT method. [Results] P. koraiensis polyphenols had relatively good inhibitory effects on A549,showing certain correlation with time or concentration. The inhibitory rare was the optimal when the concentration of P. koraiensis polyphenols was0. 4 mg / mL. Under this concentration,the inhibitory rate of extracting solution of P. koraiensis polyphenols reached 55% on lung carcinoma cell A549. P. koraiensis polyphenols showed no significant inhibitory effects on SH-SY5 Y,A375,HepG 2 or SKOV3. [Conclusions] P. koraiensis polyphenols had inhibitory effects on lung carcinoma cells.     

Effects of Volatile Oils from RADIX CURCUMAE Grown in Guangxi on the Proliferation and Apoptosis Rate of Human Hepatic Stellate Cells

[Objective]To observe the effects of extracts from RADIX CURCUMAE grown in Guangxi on the human hepatic stellate cells,and to find out its effects on proliferation and apoptosis rate. [Methods] After drug incubation of human hepatic stellate cells( HSC-LX2) for48 h,inhibition rate of cell proliferation was detected by MTT( methyl thiazolyl tetrazolium) colorimetric method; cell apoptosis in each status was measured by flow cytometry( ANNEXIN V-PE /7-AAD double staining).[Results] Extracts in low-,middle- and high-dosage groups of RADIX CURCUMAE could restrict the proliferation of HSC-LX2. The inhibitory rates at 48 h were 15. 4%,49. 1% and 58. 3%,respectively.IC50 value at 48 h was 45 μmol / L. Early and late apoptosis of cells could be induced. The percentages of total apoptotic cells at 48 h were20. 7%,29. 8% and 34. 4%,respectively.[Conclusions] Extracts from RADIX CURCUMAE grown in Guangxi could restrict the HSC-LX2 proliferation and induce the cell apoptosis,so as to affect the hepatic fibrosis. All these abilities mentioned above showed a dose-dependent manner. And these abilities in middle- and high-dosage groups of RADIX CURCUMAE were significantly stronger than those in colchicine group and compound Biejiaruangan Tablet group.     

超声波辅助浸提南瓜多糖的工艺研究

研究了超声波频率、浸提时间、固液比和提取液pH值等因素对多糖提取量的影响,并通过正交试验得到了超声波提取南瓜多糖的最佳工艺参数为:超声波频率为13kHz,提取时间15min,固液比为1g∶30mL,提取液pH值为10,在此条件下南瓜多糖的提取量为27.63mg/g。     

桔梗、白花蛇舌草不同组分对人肺癌细胞A549的抑制作用

为筛选桔梗和白花蛇舌草中的抗肿瘤活性成分,制备桔梗总黄酮、总皂苷提取物,白花蛇舌草总黄酮、总蒽醌提取物,采用亚硝酸钠-硝酸铝比色法检测总黄酮含量,香草醛法检测总皂苷含量,紫外吸收法检测总蒽醌含量,采用四甲基偶氮唑蓝比色法(MTT)检测四种提取物对人肺癌细胞A549的抑制作用。结果表明：桔梗和白花蛇舌草总黄酮含量分别为5.51% 和1.19%,桔梗总皂苷含量为1.71%,白花蛇舌草总蒽醌含量为7.81%。桔梗总皂苷浓度为0.04 mg/mL时,对人肺癌细胞A549抑制率达到87.59%,桔梗总黄酮浓度为0.5 mg/mL时,抑制率达到89.16%。白花蛇舌草总蒽醌浓度为1.2 mg/mL时,抑制率达到34.76%;白花蛇舌草总黄酮浓度为0.3 mg/mL时,抑制率达到24.33%。试验认为,桔梗总黄酮和总皂苷对肺癌细胞A549的抑制作用较强,可为抗肿瘤药物的研究提供参考。     

Inhibitiont of Obazema on Proliferation of Gastric Cancer BGC-823 Cells and Its Mechanism

[Objectives]This study aimed to investigate the inhibiting effect of Obazema on proliferation of gastric cancer BGC-823 cells and its mechanism.[Methods]BGC-823...     

马尾松松针主要活性成分提取及应用研究进展

马尾松是我国南方最主要的人工林树种之一,松针产量巨大。马尾松松针中富含挥发油、黄酮类化合物、多糖、木脂素和莽草酸等多种活性成分,具有降血糖、降血脂、抗肿瘤、抗衰老、抑菌等保健功能。简要介绍马尾松松针所含的主要活性成分及其生物学功能,并简述松针的应用领域,以促进马尾松松针的研究与应用。     

微波辅助浸提南瓜多糖的工艺研究

研究了微波功率、浸提时间、固液比和提取液pH值等因素对多糖提取量的影响,并通过正交试验设计的方法得到了微波提取南瓜多糖的最佳工艺参数为:微波功率400W,提取时间16min,固液比为1g∶50mL,提取液pH值为10,在此条件下南瓜多糖的提取量为99.6mg/g。     

棉胚珠愈伤组织诱导成纤维实验系统的研究

用棉花胚珠切块诱导愈伤组织,经悬浮振荡培养、漂浮培养、滤纸桥法等方法诱导成纤维细胞。发现漂浮培养和改进的滤纸桥法对纤维的诱导效果比悬浮振荡培养的效果好。微管解聚剂APM和核酸抑制剂抑制纤维的生长,纤维二糖有一定的促进作用。极性对纤维的生长有影响。     

棉胚珠愈伤组织诱导成纤维实验系统的研究

用棉花胚珠切块诱导愈伤组织, 经悬浮振荡培养、 漂浮培养、 滤纸桥法等方法诱导成纤 维细胞。 发现漂浮培养和改进的滤纸桥法对纤维的诱导效果比悬浮振荡培养的效果好。 微 管解聚剂APM和核酸抑制剂抑制纤维的生长, 纤维二糖有一定的促进作用。 极性对纤维的 生长有影响。     

甘啤4号大麦幼胚愈伤组织诱导及植株再生的研究

本研究以甘啤4号的幼胚为外植体材料,以模式品种Golden promise为对照,研究了不同培养基和激素配比对甘啤4号幼胚愈伤组织的诱导及植株再生的影响。研究结果表明,在同一培养基上,甘啤4号出愈率低于Golden promise,但都达到50%以上;在诱导愈伤组织的过程中,甘啤4号在IM2培养基上的出愈率较高,达71%,IM1与IM2培养基上形成的愈伤质量较IM3好,说明培养基对愈伤组织的出愈率起决定作用;且研究发现dicamba较2,4-D能提高大麦幼胚的出愈率。在相同的培养基上,Golden promise的诱导率明显高于甘啤4号,可见基因型对大麦愈伤组织的诱导率起决定作用。且不同的培养基和不同激素配比对幼胚愈伤组织的诱导率及分化率不同,分化培养基DM1的分化率高于DM2,这说明除基因型外,激素也对植株再生产生重要影响。研究证明甘啤4号愈伤组织诱导率及分化率均较高,适合于作为遗传转化的受体材料。