Sensibilisation parentérale du lapin aux protéines du lait de vache : effets sur les paramètres électrophysiologiques de l’intestin

Objectives

Study of the effect of cow's milk protein (β-lactoglobulin [β-LG] and α-lactalbumin [α-LA]) on the electrophysiological parameters of the intestine rabbits sensitized to these allergens, directly incriminated in the non immunologic allergy to cow's milk proteins and their systemic effects.

Methods

The interaction of the proteins (marked to the ¹⁴C-formol), with the mucous intestinal of parenteraly sensitized and some control rabbits, was followed-up in vitro on fragments of ileums mounted in Ussing chamber. The electrophysiological parameters which characterize the tissue to the basal state then in the time, in regular intervals, were measured after stimulation by the proteins deposed in the mucous compartment.

Results

The interaction of β-LG and α-LA with the intestinal mucous, significantly more increased among the sensitized than some control rabbits, is associated to a significant increase of the short circuit current and the difference of potential while the conductivity remain unchanged.

Conclusion

These results suggest that the interaction of the sensitizing protein with intestinal mucous caused anomalies of hydroelectrolytic transport at the immunized animals and establishes an interesting approach for the understanding of physiopathological mechanisms of the increased permeability at these proteins.     

Allergie aux protéines du lait et aux hydrolysats de protéines de lait : à propos d'une observation au diagnostic retardé

The authors report the case of an infant with a history of eczema due to cow milk and who later had two bouts of urticaria after drinking milk protein hydrolysate. The particular interest in this case is the disconnection between the onset of the eczema (when an allergy workup was not predictive) and the subsequent finding, one year later, of positive IgE-dependant sensitization tests.     

Dermatite de contact aux protéines animales due aux allergènes du chat

Contact dermatitis to animal proteins is a dermatosis that presents as chronic eczema with episodic acute exacerbations usually located on the hands and forearms. It has been described especially as a result of repeated occupational contact with livestock and foods of animal origin. The present case report describes a patient with no occupational exposure to animals but who reported regular manual contact with a cat for a few weeks before developing hand dermatitis with severe itching. The lesions did not improve with a variety of treatments, including local corticosteroids. A diagnosis of chronic hand dermatitis caused by contact with cat epithelium was suspected and confirmed by positive skin prick tests and specific IgE assay. The patient was free of symptoms when separated from his cat during a period of three weeks. A positive provocation test with a cat confirmed the diagnosis. This is the first case report of non-occupational contact dermatitis due to cat epithelium.