A simple cytochemical method for distinguishing EAC rosettes formed by lymphocytes and monocytes.

A simple cytochemical method for EAC rosettes in suspension is described which allows an easy distinction between rosettes formed by lymphocytes and monocytes. The method is based on the fact that monocytes, in contrast to lymphocytes, possess a specific cytoplasmic esterase.     

Restrictions to the use of 111indium-oxine as a radiolabel in lymphocyte migration studies

Guinea pig T lymphocytes were labelled with various concentrations of ¹¹¹In-oxine. The capacity of these cells to migrate into a skin inflammatory site was compared with that of ⁵¹Cr-labelled cells.The results show that even with low dosages of ¹¹¹In-oxine which did not impair lymph node localization (1?10 μCi/10⁸ cells/ml), migration into an inflammatory site was markedly reduced. Moreover, using this isotope, a significant contribution to the radioactivity recovered from an inflammatory site is made by the local accumulation of non-cell-bound label. These results stress the limited use of ¹¹¹In-oxine as a radiolabel in lymphocyte migration studies.