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1.
Z. Yang R. A. Culvenor R. E. Haling A. Stefanski M. H. Ryan G. A. Sandral D. R. Kidd H. Lambers R. J. Simpson 《Grass and Forage Science》2017,72(1):93-103
Temperate pasture legumes (e.g. Trifolium and Medicago spp.) often have a higher phosphorus (P) requirement for maximum productivity than pasture grasses. This is partly attributed to differences between legumes and grasses in their ability to acquire P from soil. We are the first to report differences in root morphology traits important for soil P acquisition in a range of novel pasture legumes being developed for use in temperate pastures of southern Australia. Up to a 3·6‐fold range in specific root length (SRL) (79–281 m root g?1 root) and 6·1‐fold range in root hair length (RHL) (0·12–0·75 mm) was found between the pasture species. The commonly used Trifolium subterraneum and Medicago sativa had relatively low SRLs and short root hairs, while Ornithopus compressus, O. sativus and Biserrula pelecinus had RHLs and SRLs more similar to those of two grass species that were also assessed. Specific root length was highly correlated with average root diameter, and root traits were relatively stable at different plant ages. We surmise that large differences among pasture legume species in the effective volume of soil explored could translate into significant differences in their critical P requirements (i.e. soil P concentration to achieve 90% of maximum shoot yield). 相似文献
2.
Mediterranean forage systems suffer from limited availability of fresh forage because of water deficits and extreme temperatures. Consequently, fresh forage is unavailable for at least 6–7 months a year, and farmers must buy feed to support livestock production. With the aim of overcoming these limitations, a 2‐year trial was conducted on three distinct sites in Sicily (at 10, 600 and 1200 m elevation) with thirty‐four varieties of forage species belonging to nine biennial/perennial and thirteen annual species. Results showed that by integrating grasses and legumes, species from environments with different climatic conditions enable the season of forage production to be extended from mid‐April to mid‐November. Quality traits of forage in different areas varied in relation to species and varieties. In general, the sown‐forage quality was better than in pastures and fallows in the same areas commonly used to feed animals. This also leads to a reduction in the use of supplementary feeds. Among the tested species, Lolium multiflorum and Medicago sativa emerged as the most promising for filling the forage‐deficit periods, and Trifolium spp. and Vicia sativa were found to be superior for increasing forage quality. The results are discussed in the context of adapting Mediterranean forage supplies for ensuring greater sustainability of livestock production in mountain, hill and plain areas. The proposed forage chain arrangement represents part of local potential adaptation to climate limitations and climate change. 相似文献
3.
Six dryland pastures were established at Lincoln University, Canterbury, New Zealand, in February 2002. Production and persistence of cocksfoot pastures established with subterranean, balansa, white or Caucasian clovers, and a perennial ryegrass‐white clover control and a lucerne monoculture were monitored for nine years. Total annual dry‐matter (10.0–18·5 t DM ha?1) and sown legume yields from the lucerne monoculture exceeded those from the grass‐based pastures in all but one year. The lowest lucerne yield (10 t ha?1 yr?1) occurred in Year 4, when spring snow caused ungrazed lucerne to lodge and senesce. Cocksfoot with subterranean clover was the most productive grass‐based pasture. Yields were 8·7–13·0 t DM ha?1 annually. Subterranean clover yields were 2·4–3·7 t ha?1 in six of the nine years which represented 26–32% of total annual production. In all cocksfoot‐based pastures, the contribution of sown pasture components decreased at a rate equivalent to 3·3 ± 0·05% per year (R2 = 0·83) and sown components accounted for 65% of total yield in Year 9. In contrast, sown components represented only 13% of total yield in the ryegrass‐white clover pastures in Year 9, and their contribution declined at 10·1 ± 0·9% per year (R2 = 0·94). By Year 9, 79% of the 6.6 t ha?1 produced from the ryegrass‐white clover pasture was from unsown species and 7% was dead material. For maximum production and persistence, dryland farmers on 450–780 mm yr?1 rainfall should grow lucerne or cocksfoot‐subterranean clover pastures in preference to ryegrass and white clover. Inclusion of white clover as a secondary legume component to sub clover would offer opportunities to respond to unpredictable summer rainfall after sub clover has set seed. 相似文献
4.
Under Irish conditions, the digestibility in May of grass managed for silage production is sometimes lower than expected. In each of two successive years, replicate field plots were established to examine the effects of three defoliation heights (uncut or cut to a stubble height of 10 or 5 cm) applied in winter and/or spring on herbage yields harvested in May and again in July, and on chemical composition and conservation characteristics associated with first‐cut silage. Swards that were not defoliated in December or March had a dry‐matter (DM) yield and in vitro DM digestibility (DMD) in mid‐May of 6597 kg ha?1 and 736 g kg?1, respectively, in Year 1, and corresponding values of 7338 kg ha?1 and 771 g kg?1 in Year 2. Defoliating swards to 5 cm in December reduced (P < 0·001) May DM yields compared to swards that were not defoliated in both December and March, while herbage DMD in May increased (P < 0·001) when defoliated in December or March. There were no clear effects of defoliation height or its timing on herbage ensilability or resultant conservation efficiency characteristics. The effects of defoliation on July yield were the reverse of those observed for May, while the total yield of the December and March defoliations plus the two silage harvests increased as defoliation height was lowered in Year 2 only. It is concluded that defoliation in winter and/or spring can increase herbage digestibility but will likely reduce DM yields in May. 相似文献
5.
M. L. Gatti A. T. Ayala Torales P. A. Cipriotti R. A. Golluscio 《Grass and Forage Science》2013,68(1):151-164
The objective of this work was to analyse the competitive interactions between Bromus willdenowii Kunth. (BW = prairie grass) and a perennial C 3 grass Dactylis glomerata L. (DG = cocksfoot) through morphogenetic traits, during pasture establishment. Four combinations of species (pure BW, pure DG, DG flanked by BW and BW flanked by DG plants), with and without winter nitrogen fertilization, were factorially arranged in a complete random block design. Data were recorded on three tiller age cohorts of the central target plant: the main stem and those that appeared 20 and 30 days after fertilization (daf). Leaf elongation rate (LER) of the older tillers was the only variable affected by nitrogen addition. Annual neighbours, which showed higher LER than perennial ones, caused a decrease in leaf lifespan in the younger tillers and a delay in the leaf appearance rate on the main stem and on 20‐daf tillers, and the tiller appearance rate. Annual neighbours controlled leaf and tiller dynamics and therefore restricted the vertical and horizontal space occupation of the target plant. These results reinforce the advantage of using a low seeding rate for the annual species to avoid negative effects on perennial grass establishment and persistence. 相似文献
6.
Chrizelle W. Beukes Stephanus N. Venter Emma T. Steenkamp 《Grass and Forage Science》2021,76(1):10-32
Legumes in the Fynbos vegetation of the Western Cape of South Africa have emerged as candidates for domestication, particularly for their adaptation to acidic and infertile soils. However, South African rhizobia have been shown to be very diverse and unique, and a detailed understanding of them is essential to success in forage breeding programs that seek to exploit these “new” legumes. Symbionts of legumes in South Africa that belong to traditional rhizobial genera have been shown to have a unique origin for their symbiotic loci in comparison to members sampled from other regions of the world. Some of the legume tribes in the Fynbos have also been shown to associate predominantly with unique species in the Betaproteobacterial genus Paraburkholderia. The rhizobial members of this genus have two main centres of diversity, of which South Africa is one. In this centre, the legume hosts are principally from the Papilionoideae subfamily while hosts from the mimosoid clade (now in the Caesalpinioideae) are abundant in the South American centre. Not only do these rhizobia differ in terms of host, but their symbiotic loci also show separate origins. The dominance and uniqueness of the Paraburkholderia symbionts, in the context of indigenous South African legumes, makes understanding the history and factors that affect the distribution of this genus essential if successful adaptation and effective nodulation of these legumes in Agriculture are to be achieved globally. 相似文献
7.
G. Tuñon E. Kennedy B. Horan D. Hennessy N. Lopez‐Villalobos P. Kemp A. Brennan M. O'Donovan 《Grass and Forage Science》2014,69(1):104-118
The objective of this study, which was part of a larger grazing‐systems experiment, was to investigate the cumulative impact of three levels of grazing intensity on sward production, utilization and structural characteristics. Pastures were grazed by rotational stocking with Holstein–Friesian dairy cows from 10 February to 18 November 2009. Target post‐grazing heights were 4·5 to 5 cm (high; H), 4 to 4·5 cm (intermediate; I) and 3·5 to 4 cm (low; L). Detailed sward measurement were undertaken on 0·08 of each farmlet area. There were no significant treatment differences in herbage accumulated or in herbage harvested [mean 11·3 and 11·2 t dry matter (DM) ha?1 respectively]. Above the 3·5 cm horizon, H, I and L swards had 0·56, 0·62 and 0·67 of DM as leaf and 0·30, 0·23 and 0·21 of DM as stem respectively. As grazing severity increased, tiller density of grass species other than perennial ryegrass (PRG) decreased (from 3,350 to 2,780 and to 1771 tillers m?2 for H, I and L paddocks respectively) and the rejected area decreased (from 0·27 to 0·20 and to 0·10 for H, I and L paddocks respectively). These results indicate the importance of grazing management practice on sward structure and quality and endorse the concept of increased grazing severity as a strategy to maintain high‐quality grass throughout the grazing season. The findings are presented in the context of the need for intensive dairy production systems to provide greater quantities of high‐quality pasture over an extended grazing season, in response to policy changes with the abolition of EU milk quotas. 相似文献
8.
Y. A. Torres C. A. Busso O. A. Montenegro L. Ithurrart H. D. Giorgetti G. D. Rodríguez R. E. Brevedan O. A. Fernández M. M. Mujica S. S. Baioni L. Entío M. Fioretti G. Tucat 《Grass and Forage Science》2014,69(4):580-595
The field performance of the native Pappophorum vaginatum, the naturalized Eragrostis curvula and various cultivars of the introduced Achnatherum hymenoides and Leymus cinereus was evaluated as potential forage resources in rangelands of arid Argentina during the warm seasons of 2007/2008 and 2008/2009. Plants of these grass species, obtained from seeds, were transplanted to the field in 2006, when they were 1 year old. During the study years, there were two defoliation managements: plants of all study genotypes either remained undefoliated (controls) or were defoliated twice a year during spring at 5 cm stubble height. Despite tiller number being lower (P < 0·05) on defoliated than on undefoliated plants, and total leaf length per unit basal area being similar (P > 0·05) between defoliation managements by mid‐spring, there were no differences (P > 0·05) in dry weight production between defoliated and undefoliated plants in all genotypes at the end of the second growing season. Plants of one or more of the introduced genotypes showed a similar (P > 0·05) or greater (P < 0·05), but not lower, tiller number per plant and per square centimetre, daughter tiller production, total leaf length and dry weight production per unit basal area than the native species at the end of the first and/or second growing seasons. These morphological variables were similar (P > 0·05) or greater (P < 0·05) in the native than in the naturalized genotype. Plant survival, however, was lower (P < 0·05, overall mean = 20%) in the introduced than in the native (>70%) or naturalized (>39%) genotypes at the end of the first or second growing seasons. 相似文献
9.
Å. Ergon L. Kirwan M. A. Bleken A. O. Skjelvåg R. P. Collins O. A. Rognli 《Grass and Forage Science》2016,71(4):667-682
Four‐species mixtures and pure stands of perennial ryegrass, tall fescue, white clover and red clover were grown in three‐cut and five‐cut systems at Ås, southern Norway, at a low fertilization rate (100 kg N ha?1 year?1). Over a three‐year experiment, we found strong positive effects of species diversity on annual dry‐matter yield and yield stability under both cutting frequencies. The overyielding in mixtures relative to pure stands was highest in the five‐cut system and in the second year. Among the possible pairwise species interaction effects contributing to the diversity effect, the grass–grass interaction was the strongest, being significant in both cutting systems and in all years. The grass–legume interactions were sometimes significant, but no significant legume–legume interaction could be detected. Competitive relationships between species varied from year to year and also between cutting systems. Estimations based on species identity effects and pair‐specific interactions suggested that the optimal proportions of red clover, white clover, perennial ryegrass and tall fescue in seed mixtures would have been around 0·1, 0·2, 0·4 and 0·3 in the three‐cut system, and 0·1, 0·3, 0·3 and 0·3 in the five‐cut system. 相似文献
10.
Mown herbage of timothy–meadow fescue (dry matter 218 (LDM) or 539 (HDM) g kg?1) was ensiled in laboratory silos to evaluate silage additives. For LDM silage, additives including formic acid (a blend of formic acid, sodium formate, propionic acid, benzoic acid, glycerol and another blend of formic acid and ammonium formate, both applied at 5 L t?1) were able to restrict fermentation and thereby improve intake potential of the silage. Aerobic stability (AS) of total mixed ration (TMR) was also improved. LDM grass treated with homofermentative lactic acid bacteria (hoLAB) resulted in silage containing lactic acid at 132 g kg?1 DM, ammonium‐N <40 g kg?1 total N, and pH < 3·8, and the AS was poor (<36 h). The treatment including heterofermentative strain (Lactobacillus brevis) produced more acetic acid and better AS than hoLAB. Salt treatment (sodium benzoate, potassium sorbate, sodium nitrite) reduced pH compared to the Control treatment (3·89 vs. 4·24) and improved AS of TMR. The LDM Control silage had good AS, but the TMR based on it had poor AS. All additives were able to lower pH on HDM silages also, but other benefits of using additives were minimal. The treatment including L. brevis on HDM was able to improve AS of TMR. 相似文献
11.
S. C. Somasiri P. R. Kenyon P. D. Kemp P. C. H. Morel S. T. Morris 《Grass and Forage Science》2016,71(2):270-280
A research programme was undertaken over two consecutive years with the purpose of studying the effect of herb–clover swards on lamb production performance year‐round. The focus of this study was on two consecutive late spring and early summer periods (2011, 2012). In each year, three sward treatments were compared on grazed paddocks with 40 lambs ha?1: (i) grass–clover mixture (perennial ryegrass [Lolium perenne L.] and white clover [Trifolium repens]); (ii) plantain–clover mixture (plantain [Plantago lanceolata], white clover and red clover [Trifolium pratense]); and (iii) chicory–plantain–clover mixture (plantain, chicory [Cichorium intybus L.] and white and red clovers). Lambs were weighed at 2‐week intervals, and carcass weights and GR tissue depth measurements were obtained at slaughter. In both years, lambs on treatments (ii) and (iii) had greater (P < 0·05) final live weight, liveweight gain, carcass weight, dressing‐out percentage and GR tissue depth measurements, and lower feed conversion ratio compared to lambs on treatment (i). Lamb production was similar in treatments (ii) and (iii) (P > 0·05) in each year. Therefore, during the late spring and early summer period, herb–clover mixture swards were found to be a superior option to perennial ryegrass–white clover for finishing lambs. 相似文献
12.
The short life span, irregular forage production and susceptibility to weed colonization of cool‐season grass–legume pastures are serious problems in grazing dairy systems in warm‐temperate regions. The inclusion of warm‐season species has the potential to mitigate these problems. In this study, we evaluated the effect of the inclusion of two warm‐season grasses with different growth habits on seasonal forage biomass, soil cover and weed colonization. Three different pasture mixtures were evaluated under grazing: conventional pasture (CP) [tall fescue (Festuca arundinacea), white clover (Trifolium repens) and birdsfoot trefoil (Lotus corniculatus)], CP with Paspalum dilatatum and CP with Paspalum notatum (CP + Pn). Forage biomass and soil cover were sampled thirteen times during a 3‐year trial, and sampling times were grouped by season for the analyses. The mixtures with Paspalum showed higher soil cover in the autumn, while in the winter CP had higher soil cover than CP + Pn. Competition with tall fescue was similar between mixtures with Paspalum, when considering biomass, but it was higher in CP + Pn when considering soil cover. The inclusion of P. notatum increased biomass during the autumn but decreased the mixture performance during winter by reducing tall fescue soil cover. The addition of a warm‐season grass species with a moderate competing ability like P. dilatatum is likely to avoid a negative impact on the cool‐season component of the pasture. 相似文献
13.
G. X. Liu Y. J. Zhang K. A. Hovstad P. S. Mao J. G. Han 《Grass and Forage Science》2014,69(3):479-487
A two‐year small‐plot field experiment was carried out to investigate the competitive relationship between seedlings of two important forage grasses, Leymus chinensis and Bromus inermis, under three levels of gap size (distance between seedlings and their neighbours) of 10, 20 and 40 cm in diameter, with neighbouring roots present or absent. Seeds of the two species were sown into artificially created gaps, in either monoculture or mixtures at equal rates. Results indicated that gap size had significant effects on seedling emergence, seedling survival and the growth performance of the two species, grown alone or in a mixture. Excluding the roots of neighbouring plants enhanced all the measured attributes and had significant effects on both the number of inflorescences and the biomass of B. inermis and on the biomass of L. chinensis. The performance of the two species differed significantly, both alone and in mixtures, with B. inermis outperforming L. chinensis consistently. The number of inflorescences of B. inermis was 2·6‐fold higher than L. chinensis when grown in a mixture, and L. chinensis displayed a large and significant reduction in biomass when grown in a mixture with B. inermis (5·7 g plot?1) as compared with a monoculture (25·9 g plot?1). The relative yield (RY) for B. inermis exceeded 0·5, but for L. chinensis, it was below 0·5 in all treatments, and aggressivity (A) in all treatments was above 0. In addition, the relative competition intensity (RCI) of B. inermis was less than that of L. chinensis in all treatments, both separately and when averaged across treatments. We conclude that B. inermis is a superior competitor to L. chinensis in gaps, whereas the performance of L. chinensis was greatly suppressed in mixtures. This indicated that under similar conditions, B. inermis outcompetes L. chinensis during early growth stages. The results have practical implications for the restoration and revegetation of grasslands in northern China. 相似文献
14.
P. H. F. Michel L. C. Gonçalves J. A. S. Rodrigues K. M. Keller V. S. Raposo E. M. Lima F. P. C. Santos D. G. Jayme 《Grass and Forage Science》2017,72(3):432-440
Re‐ensiling of previously ensiled forage has been a common practice in Brazil, and the use of inoculants may provide a means of reducing dry‐matter (DM) loss. This study aimed to determine the effect of re‐ensiling and the use of microbial inoculants on the quality of sorghum silage. Treatments were presence/absence of an inoculant (Lactobacillus plantarum and Propionibacterium acidipropionici) in the silage, and the re‐ensiling, or not, of the material after 24 h of exposure to air, and these were tested in a factorial 2 × 2 design. Losses due to gas, effluent and total DM were assessed, as were the fermentation characteristics, chemical composition, aerobic stability, and aerobic counts of microorganisms. Effluent loss was higher in re‐ensiled silage, and these silages had lower lactic acid content and higher levels of acetic and propionic acids. The in vitro DM digestibility was lower in the re‐ensiled sorghum silages. The re‐ensiled silage had higher aerobic stability. The inoculant only increased the acetic acid content of the silage. The re‐ensiling of sorghum silage increased effluent loss by 71·2%, and reduced DM digestibility by 5·35%. The use of inoculant did not influence the quality of sorghum silage. 相似文献
15.
Š. Supek V. Pavlů L. Pavlů J. Gaisler M. Hejcman V. Ludvíková J. Mikulka 《Grass and Forage Science》2017,72(3):516-523
Dandelion (Taraxacum officinale agg.) is a common forb species in grasslands in Europe. Although sometimes regarded as a valuable forage herb, it may become a weed, especially in arable land. There is limited information on the response of Taraxacum to long‐term grassland management practices. Therefore, we analysed cover and dry‐matter standing biomass of Taraxacum in a long‐term (1998–2012) grazing experiment on an Agrostis capillaris grassland. The following treatments were laid out on formerly abandoned grassland: (i) intensive grazing (IG); (ii) extensive grazing (EG); (iii) first cut followed by intensive grazing (ICG); (iv) first cut followed by extensive grazing (ECG); and (v) unmanaged grassland (U). During the first 10 years, all defoliation treatments (i–iv) supported the presence of Taraxacum, and the lowest proportion was recorded in the unmanaged treatment (U). During the final 7 years of the study, combined cutting and grazing promoted Taraxacum cover more than that of grazing only (ICG > IG > ECG > EG). Cover of Taraxacum was negatively affected by increasing sward height where Taraxacum plants had lower fitness. Due to the relatively strong relationship between percentage cover of Taraxacum and its dry‐matter biomass, percentage cover could be used as a simple method for the assessment of biomass of Taraxacum in a sward. Results are discussed in the context of adapting the management of A. capillaris grassland as a simple method for control of Taraxacum abundance, particularly in situations of extensification or abandonment. 相似文献
16.
K. Z. Mganga N. K. R. Musimba D. M. Nyariki M. M. Nyangito A. W. Mwang'ombe 《Grass and Forage Science》2015,70(1):161-167
Livestock production is the main source of livelihood in the arid and semi‐arid lands in Africa. However, desertification characterized by vegetation degradation and soil erosion is a major threat to the sustainability of land‐based production systems. Native rangeland forage species Cenchrus ciliaris L. (Buffel grass/African foxtail grass), Eragrostis superba Peyr. (Maasai love grass) and Enteropogon macrostachyus (Hochst. Ex A. Rich.) Monro ex Benth. (Bush rye grass) have been used to combat desertification. The objectives of the study were to identify the best‐suited native grass species to combat desertification in a semi‐arid environment in Kenya and to identify the preferred grass species among the agropastoralists in the area. Percentage basal cover, plant densities and frequencies of the three grasses in pure stands and mixtures were estimated. Grass species preferences were through household survey and focus group discussion. Results showed a significant difference (P < 0·05) in plant densities and cover estimates: E. macrostachyus was ranked first; C. ciliaris and E. superba were ranked second and third respectively. The agropastoral farmers, however, preferred E. superba followed by C. ciliaris and E. macrostachyus, a reverse trend. These results suggest that the choice of grass species to combat desertification is influenced more by its contribution as a source of forage for livestock than its contribution for rehabilitation purposes. 相似文献
17.
Å. Ergon L. Kirwan G. Fystro M. A. Bleken R. P. Collins O. A. Rognli 《Grass and Forage Science》2017,72(2):333-342
Mixtures and pure stands of perennial ryegrass, tall fescue, white clover and red clover were grown in a three‐cut and a five‐cut system in southern Norway, at a low fertilization rate (100 kg N ha?1 year?1). The nutritional quality (annual weighted averages) of the dried forage from the two‐first harvesting years was analysed. There was no significant effect of species diversity on crude protein (CP) concentration. In the three‐cut system, we found a significant species diversity effect leading to 10% higher concentrations of acid detergent fibre (ADF), 20–22% lower concentrations of water‐soluble carbohydrate (WSC) and 4% lower net energy for lactation (NEL) concentrations in mixtures compared with pure stands (averaged across the two‐first years). In the five‐cut system, similar effects were seen in the first year only. This diversity effect was associated with a reduction in WSC and NEL concentrations and an increase in ADF, NDF and CP concentrations in the grass species, and not in red clover, when grown in mixtures. This is thought to be a combined result of better N availability and more shading in the mixtures. Species diversity reduced the intra‐annual variability in nutritional quality in both cutting systems. 相似文献
18.
In the UK, upland hay meadows (Anthoxanthum odoratum–Geranium sylvaticum grassland) with high plant biodiversity are rare and confined to submontane areas of northern England. We report results from a 5‐year experiment to test suggestions that recent biodiversity declines were attributable to increased sheep‐stocking density and a longer spring stocking period, thus delaying the shut up date for the growth of the hay crop. Longer stocking periods and higher stocking densities decreased the forage mass at 8 July, but they increased herbage N content and digestibility, reduced plant species diversity and reduced populations and seed production of Rhinanthus minor. Compared with unstocked swards, the similarity of the vegetation to Anthoxanthum odoratum–Geranium sylvaticum grassland was reduced by 16·9% when stocking with sheep continued until 27 May each year, and by 8·3% when sward heights were maintained at 3 cm compared with 5 cm. Increased mean sward height and height of R. minor were positively correlated with accumulated temperatures. Results support suggestions that recent reductions in the nature value of these grasslands might be a consequence of high stocking densities persisting until later in the spring, carried out during a 1‐year period with warmer temperatures. 相似文献
19.
M. J. S. Lopes M. B. Dias‐Filho T. H. R. Castro G. B. Silva 《Grass and Forage Science》2018,73(2):493-499
This is the first report on the effect of light intensity and plant growth‐promoting rhizobacteria (PGPR) on the growth of a tropical forage grass, being a relevant study to improve pasture management in conventional farming and integrated crop‐livestock‐forestry systems. In this study, our aim was to evaluate the effects of light intensity and Burkholderia pyrrocinia and Pseudomonas fluorescens inoculation on Brachiaria brizantha cv. BRS Piatã growth, and phenotypic plasticity to shade. The experiment was conducted in a semi‐controlled environment. Seedlings of B. brizantha were allocated to full sun and shade. P. fluorescens and B. pyrrocinia were inoculated individually or co‐inoculated by soil drench, 14 days after seedling emergence. We evaluated morphogenesis, structural and growth parameters. Irrespective of the light regime, co‐inoculated plants had greater leaf area and SPAD index (chlorophyll content). Increase in total biomass production in co‐inoculated plants was over 100% and 300%, under full sun and shade respectively. Co‐inoculated P. fluorescens and B. pyrrocinia increased shade tolerance in B. brizantha, improving plant performance. Co‐inoculation promoted growth in B. brizantha under both sun and shade, indicating its potential as a bio‐fertilizer in conventional and integrated systems, especially in silvopastoral systems, where light availability to pasture growth may be limited. 相似文献
20.
Leymus chinensis is an important grass in China and Russia. Six lactic acid bacteria (LAB) strains (LB, LPL1, LPL2, LPL3, LCL and WH) from L. chinensis silage were screened and identified and their effects on fermentation quality were investigated. All six strains were grown at 6·5% NaCl and pH 4·00. Strains LPL1, LPL2 and LPL3 were identified as Lactobacillus plantarum, and LB, WH and LCL were classified as Lactobacillus brevis, Weissella hellenica and Lactobacillus casei respectively. The six isolated strains and a commercial inoculant (Lactobacillus buchneri) were added to L. chinensis for ensiling at densities of 500 and 600 kg m?3. The control was sprayed with the same volume of distilled water. The effects of the strains on fermentation quality after 45 d ensiling and aerobic stability during 8 d of exposure to air were evaluated. The 600 kg m?3 silage had lower pH, butyric acid, ammonia nitrogen content and coliform bacteria counts than the 500 kg m?3 density silage (P < 0·05). The six isolated strains decreased pH, butyric acid content and increased lactic acid content, and all inoculants increased L. chinensis silage aerobic stability except LCL (P < 0·05). The fermentation quality of L. chinensis silage increased with higher ensiling density. The LAB strains improved the fermentation quality, and high‐quality silage could be obtained at low ensiling density with the addition of the LAB strains. The strains improved the aerobic stability; Lb. buchneri and Lb. brevis showed the best performance. 相似文献