口腔扁平苔藓患者红细胞免疫粘附功能与循环免疫复合物的初步…

本文旨在探讨口腔扁平苔藓（ＯＬＰ）患者红细胞免疫粘附功能与ＯＬＰ发病过程的关系。对８０名ＯＬＰ患者和６０名健康对照组的Ｃ３ｂ花环、免疫复合物花环、循环免疫复合物三个项目进行检测，结果ＯＬＰ组红细胞Ｃ３ｂ受体花环率和红细胞免疫复合物花环率降低，循环免疫复合物阳性率上升，与对照组比较有显著性差异。提示ＯＬＰ患者的红细胞免疫粘附功能减退可能与ＯＬＰ的发病过程有关。     

口腔扁平苔藓患者红细胞免疫粘附功能初探

采用我国郭氏酵母菌花环法对30例口腔扁平苔藓(OLP)患者及30例健康对照者红细胞免疫粘附功能进行了初步观察,以了解红细胞是否参与了 OLP 的免疫过程。结果发现,OLP 患者红细胞 C3b受体花环率及循环免疫复合物(CIC)花环率均较健康对照组升高。说明 OLP 患者红细胞免疫粘附功能较正常人增强,推测这可能是由于 CIC 增高后红细胞免疫功能的代偿性反应。这对于防止 CIC 在局部沉积造成体液免疫性损伤具有重要意义,为 OLP 细胞介导的免疫损伤提供了又一证据。     

口腔扁平苔藓患者红细胞免疫粘附功能的初步研究

本文采用国内郭氏酵母菌花环法对６０例口腔扁平苔藓（ＯＬＰ）患者及３０例正常对照者红细胞免疫粘附力功能进行了测定，以探讨红细胞免疫粘附功能与ＯＬＰ发病过程的关系，结果发现，ＯＬＰ患者红细胞Ｃ３ｂ受体花环率明显低于正常对照组，而红细胞免疫复合物花环率明显高于正常对照组，说明ＯＬＰ患者存在继发性红细胞免疫功能低下。     

口腔扁平苔藓氯喹治疗前后红细胞免疫粘附功能和循环免疫复合物的观察

本实验对30例口腔扁平苔藓（OLP）患者使用氯喹（CQ）前后及30名健康人进行了红细胞免疫粘附功能（RCIA）和循环免疫复合物（CIC）检测，结果显示：未经治疗的OLP组红细胞C3b受体花环率和红细胞免疫复合物花环率降低，循环免疫复合物水平上升，与健康对照组比较有显著性差异；经CQ治疗后的OLP组红细胞C3b受体花环率和红细胞免疫复合物花环率较治疗前上升，经统计学处理有显著性差异，与健康对照组比较无显著性差异，循环免疫复合物水平较治疗前下降，与健康对照组比较无显著性差异。结果提示：改善红细胞免疫粘附功能可能为CQ治疗OLP的机理之一。     

红细胞免疫粘附功能检测与口腔扁平苔藓病因关系的初步研究

目的 探讨红细胞免疫粘附功能与口腔扁平苔藓病因的关系。方法 采用酵母茵花环法对42例口腔扁平苔藓患者及30例健康对照者红细胞免疫粘附功能进行了测定。结果口腔扁平苔藓组红细胞C3b受体花环率明显低于对照组,而红细胞免疫复合物花环率及循环免疫复合物明显高于对照组。结论 口腔扁平苔藓病的病因与代偿性红细胞免疫粘附功能减退有关。     

口腔扁平苔藓与糖尿病及循环免疫复合物关系的研究

口腔扁平苔藓(OLP)是一种慢性炎症性残病,其病因和发病机理不明。近年来随着免疫学的发展,目前已基本上明确OLP病损的粘膜固有层中浸润细胞主要是T淋巴细胞。细胞免疫在该病发病中起主要作用。近年来有一些学者曾报导OLP与一些系统病有关,特别是OLP与糖尿病的关系,但国内外多数学者报导很不一致。其中lunstrom1983年曾报导40名OLP患者中有11名患有糖尿病(28％),而国内陈海琼,对609例OLP患者经检查仅7例患有糖尿病(1.15％)。二者差距很大。     

口腔扁平苔藓氯喹治疗前后红细胞免疫粘附功能和循环免疫复 …

本实验对３０例口腔扁平苔藓（ＯＬＰ）患者使用氯喹（ＣＱ）前后及３０名健康人进行了红细胞免疫粘附功能（ＲＣＩＡ）和循环免疫复合物（ＣＩＣ）检测，结果显示，未经治疗的ＯＬＰ组红细胞Ｃ３ｂ受体花环率和红细胞免疫复合物花环率降低，循环免疫复合物水平上升，与健康对照组比较有显著性差异，经ＣＱ治疗后的ＯＬＰ组红细胞Ｃ３ｂ受体花环率和红细胞免疫复合物花环率较治疗前上升统计学处理有显著怀差异，与健康对照组比较无显     

牙周炎患者红细胞免疫粘附功能的初步研究

采用酵母菌花环法对３１例牙周炎患者红细胞的免疫粘附功能进行了初步检测，并与正常组比较，结构显示：牙周炎患者红细胞Ｃ３ｂ受体活性稍降低但与正常组无显著性差异（Ｐ＞０．０５），红细胞膜免疫复合物升高且与正常组有非常显著性差异（Ｐ＜０．０１）．并对免疫功能的变化机制进行了讨论。     

口腔扁平苔藓患者免疫功能的实验研究

目的 探讨口腔扁平苔藓（OLP）的发病机理。方法 对口腔扁平苔藓患者64例及健康成人31例,采用微量全血^3H－TdR掺入法检测T淋巴细胞增殖功能;酵母菌花环法检测红细胞免疫粘附功能;采用单向免疫扩散法测定血清中免疫球蛋白IgG、IgM、IgA及补体C3、C4含量。结果 OLP患者较健康成人外周血T淋巴细胞增殖功能显著降低,每分钟脉冲数（CPM）（P＜0．05）,刺激指数（SI）（P＜0．001）;红细胞C3b受体花环形成率（RBC－C3bRR）显著下降（P＜0．01）,红细胞免疫复合物花环形成率（RBC－ICR）无明显差异;血清中C3、C4含量无明显差异,IgA、IgG含量显著降低（P＜0．001）,而IgM含量则明显升高（P＜0．01）。结论 OLP的发生可能与机体免疫功能紊乱有关。     

补血活血法调节口腔扁平苔藓患者红细胞免疫功能的研究

目的：探讨补血活血中药对口腔扁平苔藓(OLP)患者红细胞免疫功能即红细胞-C3b受体花环率(RBC-C3bRR)、红细胞免疫复合物花环率(RBC-ICR)的调节作用，为临床用药提供依据。方法：50例OLP患者随机分为2组，分别给予补血活血中药和雷公藤总甙片进行治疗．采用酵母多糖法检测治疗前后RBC-C3bRR、RBC-ICR的变化。结果：治疗2个月后补血活血中药对OLP患者的黏膜斑纹改善方面明显优于雷公藤总甙片；可提高RBC-3bRR．降低RBC-ICR．从而调节红细胞免疫功能：还显示患者黏膜充血及白斑程度越重，RBC-ICR就越高。结论：OLP患者因病情不同红细胞免疫低下的程度也不同：补血活血中药是治疗OLP有效的并具有凋节红细胞免疫功能的药物。     

口腔扁平苔藓患者血清可溶性白细胞介素2受体水平与红细胞免疫功能相关性的研究

目的　研究口腔扁平苔藓（ Ｏ Ｌ Ｐ） 患者血清可溶性白细胞介素２ 受体（ Ｓ Ｉ Ｌ－ ２ Ｒ） 水平与红细胞免疫功能相关性。方法　采用双抗体夹心 Ｅ Ｌ Ｉ Ｓ Ａ 法检测３０ 例 Ｏ Ｌ Ｐ 患者及３０ 例正常对照者血清 Ｓ Ｉ Ｌ－ ２ Ｒ 的含量, 采用郭峰酵母菌花环法检测其 Ｒ Ｂ Ｃ－ Ｃ３ｂ Ｒ 花环率、 Ｒ Ｂ Ｃ－ Ｉ Ｃ 花环率。结果　 Ｏ Ｌ Ｐ 患者血清 Ｓ Ｉ Ｌ－ ２ Ｒ 含量、 Ｒ Ｂ Ｃ－ Ｉ Ｃ花环率明显高于正常对照组, 而 Ｒ Ｂ Ｃ－ Ｃ３ｂ Ｒ 花环率明显低于正常对照组。患者 Ｓ Ｉ Ｌ－ ２ Ｒ 与 Ｒ Ｂ Ｃ－ Ｃ３ｂ Ｒ 花环率呈明显负相关, 而与 Ｒ Ｂ Ｃ－ Ｉ Ｃ 花环率明显正相关。结论　过高的 Ｓ Ｉ Ｌ－ ２ Ｒ 水平可能会抑制 Ｏ Ｌ Ｐ 患者的红细胞免疫功能。     

辅助性T淋巴细胞极化相关细胞因子在口腔扁平苔藓中的研究进展

口腔扁平苔藓是一种病因不明的皮肤黏膜慢性免疫炎症性疾病,辅助性T淋巴细胞(Thcell)在口腔扁平苔藓的发病机制中起到了重要作用。近年来,口腔扁平苔藓中Th细胞的极化状态引起了较为广泛的关注。研究表明,Th细胞极化相关细胞因子的变化与口腔扁平苔藓的发病密切相关。下文就这些细胞因子在口腔扁平苔藓中的研究进展作一综述。     

玉屏风对口腔扁平苔藓患者免疫调节的临床分析

目的：探讨玉屏风对口腔扁平苔藓（OLP）的免疫调节作用。方法：88例OLP患者随机分为实验组45例。口服玉屏风口服液加局部治疗,对照组43例,口服维生素A与维生素E加局部治疗。观察疗效并检测治疗前后血清IgG、IgM、IgA、C3、C4的变化。结果：实验组总有效率95．56％,对照组总有效率81．40％,两者有显著性差异（P〈0．05）,实验组治疗前后IgG、IgA、C3、CA指数变化有显著性差异（P〈0．05或P〈0．01）。对照组治疗前后各项免疫指标变化无显著性差异。结论：玉屏风对OLP患者血清中IgG有双向调节作用,并能下调IgA和补体C3、C4。     

Abnormal EBV immune status in oral lichen planus

OBJECTIVE: To investigate any possible association between oral lichen planus (OLP) and the humoral immune response to Epstein-Barr virus (EBV). MATERIAL AND METHODS The population consisted of 22 patients with histologically verified OLP and 22 healthy sex- and age-matched controls without any oral mucosal diseases. Specific serum IgG antibodies (Ab) towards EBV early antigen (EA) and nuclear antigen-I (EBNA) and IgM Ab towards EBV EA were determined by ELISA.
RESULTS: The optometric density (OD) IgG anti-EA Ab levels were significantly higher in OLP patients than in controls and a significantly negative correlation between duration of symptoms from OLP and IgG anti-EA OD Ab values was demonstrated. IgM anti-EA and IgG anti-EBNA OD Ab levels were not significantly different between the two groups.
CONCLUSION: The hypothesis of any possible association between EBV and OLP has never been tested but the present results seem to lend support for an aberration in the humoral response to EBV in OLP patients.     

A clinical study of 674 patients with oral lichen planus in China

BACKGROUND: Oral lichen planus (OLP) is a chronic inflammatory disease with different clinical presentations that can be classified as reticular, atrophic or erosive. Although OLP is a relatively common disorder, the reports comprising large numbers of OLP patients with specific character are lacking in the literature. The purpose of this paper was to describe the clinical characteristics of OLP in 674 Chinese patients. METHODS: A total of 674 charts of patients with histologically confirmed OLP were collected from Stomatological Hospital of Wuhan University between 1963 and 2003. RESULTS: Of the 674 patients, 65.9% were women and 34.1% were men. The most common clinical presentation was the reticular type (51.3%), and symptomatic OLP was noted in 67.5% of the patients, mainly in those with the erosive form. The erosive presentations showed significantly longer duration, more sites affected and a much greater old patients predominance than reticular or atrophic ones. About 90.9% of the patients had multiple oral sites of involvement and isolated lower lip lichen planus were observed in 60 cases (8.9%) and isolated gingiva lichen in only one case (0.2%). Skin involvement of lichen planus was found in 11.4% of patients. No statistically significant differences could be identified between OLP and diabetes, cardiovascular disease, smoking or alcohol use. Precipitating factors that resulted in an exacerbation of the disease were frequently noted and included foods, stress, dental cusp and poor oral hygiene. The transformation of OLP into malignancy was observed in four patients at sites previously diagnosed by clinical examination as erosive or atrophic lichen planus. CONCLUSIONS: Patients with OLP in China usually present with distinctive clinical morphology and characteristic distribution and few may display lesions with a confusing array of forms mimicking other diseases. A long time follow up is of utmost importance to detect its malignant transformation.     

口腔扁平苔藓患者血硒水平的测定及初步研究

目的探索口腔扁平苔藓与必需微量元素硒的关系。方法30例口腔扁平苔藓实验组与30例健康对照组,采用原子吸收分光光度法测定红细胞及血清硒水平。结果实验组红细胞及血清硒水平均低于健康对照组（P＜0.05）。结论硒水平低下可能是口腔扁平苔藓发病原因之一。     

Immunohistochemical study of oral keratoses including lichen planus

Biopsies of non-ulcerated oral mucosa from 13 patients with oral lichen planus and 12 patients with leukoplakia were immunohistochemically stained using monoclonal antibodies to pan T, pan B, T helper and T suppressor/cytotoxic cells and the stained lymphocytes enumerated using an image analyser. The results show the preponderance of T cells infiltrating both oral lichen planus and leukoplakia. The T helper: T suppressor/cytotoxic cell ratio was the same (1:2) for both oral lichen planus and leukoplakia. A similar proportion of T suppressor/cytotoxic cells was found infiltrating the epithelium. These data indicate that T cell subset analysis is of no value in distinguishing oral lichen planus from other oral keratoses.     

EP—CAM在OLP和OSCC组织中的表达及意义

目的：观察上皮细胞黏附分子（Ep—CAM）在口腔正常黏膜、扁平苔癣（OLP）和鳞癌（OSCC）组织中表达的变化,分析Ep—CAM在口腔黏膜疾病及其癌变发病机制中的作用。方法：采用免疫组织化学法检测10例正常口腔黏膜、30例OLP（15例非糜烂型、15例糜烂型）及20例OSCC组织中Ep—CAM的表达变化。结果：Ep—CAM在正常黏膜组织阴性表达,而在非糜烂型OLP、糜烂型OLP及OSCC组织中表达逐渐增强。结论：Ep—CAM在口腔黏膜癌变的发展过程中可能具有一定的指示意义。     

口腔扁平苔藓免疫学因素的研究现状

口腔扁平苔藓(OLP)是一种严重影响患者身心健康的发生于口腔黏膜的非传染性炎症性疾病.OLP的病因尚不明确,可能与多种因素有关.目前的研究表明,OLP的发病与免疫因素密切相关,它是一种以T淋巴细胞介导的免疫应答为特征的慢性疾病.抗原特异性机制和非特异性机制可能参与OLP的免疫过程.首先由树突状细胞摄取、处理未知抗原触发...     

Associations between mast cells and laminin in oral lichen planus

Mast cell numbers are increased significantly in oral lichen planus (OLP). In other inflammatory conditions, mast cells frequently adhere to extracellular matrix proteins such as laminin. The aim of this study, therefore, was to determine whether the distribution of mast cells in OLP is related topographically to laminin in vascular and epithelial basement membranes. Monoclonal antibodies for tryptase, laminin and the α6β1 CD49f laminin-binding integrin were used to identify mast cells, basement membranes (blood vessels and basal epithelium) and the "classical" laminin adhesion receptor, respectively. A double-labelling immunoperoxidase technique was employed to examine and compare mast cell-laminin relationships in OLP (n=19) and normal buccal mucosa (NBM, n=13). In both OLP and NBM, the majority of mast cells were located close to vascular basement membranes. Quantitative studies revealed that the number of mast cells associated with the laminin of vascular basement membranes (distance <1 μm) was two-fold and three-fold higher, respectively, in the superficial and deep layers in OLP compared with NBM (P<0.001). The frequency distribution of mast cells associated with basal epithelium was not statistically different in both groups (P>0.05). The association of mast cells with laminin may be an important determinant of mast cell density in OLP. During OLP lesion formation and progression, the preferential distribution of mast cells in the immediate perivascular region provides an ideal situation for mast cell-derived mediators to influence the vascular endothelium.