CHARACTERIZATION OF A HUMAN HERPES VIRUS－6（HHV－6） AND EPSTEIN－BARR VIRUS（EBV） ASSOCIATED LEUKEMIC CELL LINE，J6－1

ＣＨＡＲＡＣＴＥＲＩＺＡＴＩＯＮＯＦＡＨＵＭＡＮＨＥＲＰＥＳＶＩＲＵＳ－６（ＨＨＶ－６）ＡＮＤＥＰＳＴＥＩＮ－ＢＡＲＲＶＩＲＵＳ（ＥＢＶ）ＡＳＳＯＣＩＡＴＥＤＬＥＵＫＥＭＩＣＣＥＬＬＬＩＮＥ，Ｊ６－１ＷｕＫｅｆｕ吴克复；ＪａｎｏｓＬｕｋａ；Ｓｈａｎｔ...     

The Paper Symposium on PLC VIRAL HEPATITIS（HBV AND HCV）BACKGROUND IN CHINESE PATIENTS WITH HEPATOCELLULAR CARCINOMA

ＴｈｅＰａｐｅｒＳｙｍｐｏｓｉｕｍｏｎＰＬＣＶＩＲＡＬＨＥＰＡＴＩＴＩＳ（ＨＢＶＡＮＤＨＣＶ）ＢＡＣＫＧＲＯＵＮＤＩＮＣＨＩＮＥＳＥＰＡＴＩＥＮＴＳＷＩＴＨＨＥＰＡＴＯＣＥＬＬＵＬＡＲＣＡＲＣＩＮＯＭＡＹｕＺｈｕｙｕａｎ；余竹元；ＴａｎｇＺｈａｏｙｏ...     

HEPATITIS B AND C VIRUSES AND THEIR INTERACTION IN THE PTHOGENESIS OF HEPATOCELLULAR CARCINOMA

王树林，张耀铮ＨＥＰＡＴＩＴＩＳＢＡＮＤＣＶＩＲＵＳＥＳＡＮＤＴＨＥＩＲＩＮＴＥＲＡＣＴＩＯＮＩＮＴＨＥＰＴＨＯＧＥＮＥＳＩＳＯＦＨＥＰＡＴＯＣＥＬＬＵＬＡＲＣＡＲＣＩＮＯＭＡ￥ＷａｎｇＳｈｕｌｉｎ；ＺｈａｎｇＹａｏｚｈｅｎｇ；（Ｄｅｐａｒｔｍｅｎｔ...     

EVALUATION OF REVERSE PASSIVE HEMAGGLUTINATION （RPHA） FECAL OCCULT BLOOD TEST IN SCREENING OF COLORECTAL NEOPLASIA

ＥＶＡＬＵＡＴＩＯＮＯＦＲＥＶＥＲＳＥＰＡＳＳＩＶＥＨＥＭＡＧＧＬＵＴＩＮＡＴＩＯＮ（ＲＰＨＡ）ＦＥＣＡＬＯＣＣＵＬＴＢＬＯＯＤＴＥＳＴＩＮＳＣＲＥＥＮＩＮＧＯＦＣＯＬＯＲＥＣＴＡＬＮＥＯＰＬＡＳＩＡＹｕＨａｉ；ＺｈｏｕＬｕｎ；ＺｈｅｎＢｅｉｙｉ；Ｑ...     

IMMUNOLOGICAL CHARACTERISTICS OF THE LEUKEMIA CELLS TRANSFECTED WITH ONCOSTATIN M GENE BY RECOMBINANT ADENOVIRUS

ＩＭＭＵＮＯＬＯＧＩＣＡＬＣＨＡＲＡＣＴＥＲＩＳＴＩＣＳＯＦＴＨＥＬＥＵＫＥＭＩＡＣＥＬＬＳＴＲＡＮＳＦＥＣＴＥＤＷＩＴＨＯＮＣＯＳＴＡＴＩＮＭＧＥＮＥＢＹＲＥＣＯＭＢＩＮＡＮＴＡＤＥＮＯＶＩＲＵＳ１ＷａｎｇＱｕａｎｘｉｎｇ王全兴ＣａｏＸｕｅｔａｏ２...     

RESEARCH ON SERUM LEVELS OF RETINOL，α－TOCOPHEROL β－CAROTENE，AND 12 ELEMENTS IN GASTRIC DYSPLASIA AND GASTRIC CANCER PATIENTS

ＲＥＳＥＡＲＣＨＯＮＳＥＲＵＭＬＥＶＥＬＳＯＦＲＥＴＩＮＯＬ，α－ＴＯＣＯＰＨＥＲＯＬβ－ＣＡＲＯＴＥＮＥ，ＡＮＤ１２ＥＬＥＭＥＮＴＳＩＮＧＡＳＴＲＩＣＤＹＳＰＬＡＳＩＡＡＮＤＧＡＳＴＲＩＣＣＡＮＣＥＲＰＡＴＩＥＮＴＳＣｈｅｎｇＷｕｆｅｎｇ；程五凤；...     

THE EFFECT OF ACTIVE COMPONENTS OF LYCIUM BARBARUM AND GARLIC（LB－GO）ON THE SYNTHESIS OF DNA AND ULTRASTRUCTURE OF U_（14） CERV

ＴＨＥＥＦＦＥＣＴＯＦＡＣＴＩＶＥＣＯＭＰＯＮＥＮＴＳＯＦＬＹＣＩＵＭＢＡＲＢＡＲＵＭＡＮＤＧＡＲＬＩＣ（ＬＢ－ＧＯ）ＯＮＴＨＥＳＹＮＴＨＥＳＩＳＯＦＤＮＡＡＮＤＵＬＴＲＡＳＴＲＵＣＴＵＲＥＯＦＵ_（１４）ＣＥＲＶＩＸＣＡＮＣＥＲＣＥＬＬＳＩＮＭＩＣ?..     

EXPRESSIONS OF ESTROGEN OCCUPIED RECEPTOR（EoR） AND PROGESTERONEOCCUPIED RECEPTOR（PoR） AND C－erbB－2ONCOPROTEININ HUMANNASOPHAR

ＥＸＰＲＥＳＳＩＯＮＳＯＦＥＳＴＲＯＧＥＮＯＣＣＵＰＩＥＤＲＥＣＥＰＴＯＲ（ＥｏＲ）ＡＮＤＰＲＯＧＥＳＴＥＲＯＮＥＯＣＣＵＰＩＥＤＲＥＣＥＰＴＯＲ（ＰｏＲ）ＡＮＤＣ－ｅｒｂＢ－２ＯＮＣＯＰＲＯＴＥＩＮＩＮＨＵＭＡＮＮＡＳＯＰＨＡＲＹＮＧＥＡＬＣＡＲＣ...     

PROLIFERATION OF ANTI－CD_3 McAb AND IL－2 INDUCED SPLENOCYTES AND ANTITUMOR EFFECT OF THEIR CULTURE SUPERNATANTS

ＰＲＯＬＩＦＥＲＡＴＩＯＮＯＦＡＮＴＩ－ＣＤ_３ＭｃＡｂＡＮＤＩＬ－２ＩＮＤＵＣＥＤＳＰＬＥＮＯＣＹＴＥＳＡＮＤＡＮＴＩＴＵＭＯＲＥＦＦＥＣＴＯＦＴＨＥＩＲＣＵＬＴＵＲＥＳＵＰＥＲＮＡＴＡＮＴＳ￥ＳｈｅｎＧｕａｎｘｉｎ；沈关心；ＷａｎｇＸｉａｌｉｎ；?..     

ANTI-METASTATIC EFFECT OF ONCOLYSATES FROM MURINE MELANOMA CELLS TRANSFECTED WITH RECOMBINANT VACCINIA VIRUS ENCODING HUMAN IL-2

ＡＮＴＩＭＥＴＡＳＴＡＴＩＣＥＦＦＥＣＴＯＦＯＮＣＯＬＹＳＡＴＥＳＦＲＯＭＭＵＲＩＮＥＭＥＬＡＮＯＭＡＣＥＬＬＳＴＲＡＮＳＦＥＣＴＥＤＷＩＴＨＲＥＣＯＭＢＩＮＡＮＴＶＡＣＣＩＮＩＡＶＩＲＵＳＥＮＣＯＤＩＮＧＨＵＭＡＮＩＬ２１ＷａｎＴａｏ万涛Ｃａｏ...     

Cytomegalovirus pneumonia prior to engraftment following T-cell depleted bone marrow transplantation

CMV pneumonia is a frequent complication of allogeneic bone marrow transplantation (BMT). It usually appears 23 months following transplantation and is associated with a high mortality rate. The incidence of CMV pneumonia in our T-lymphocyte depleted allogeneic BMT recipients, transplanted between 1987–1991, was 18 out of 197 (9.2%) patients. In 3 patients (1.5% of allogeneic BMT recipients), pneumonia occurred prior to marrow engraftment, on days 12–16 post BMT. These patients did not develop acute GVHD in contrast to 9/11 patients who had acute GVHD in addition to developing CMV pneumonia between engraftment and day +100 (p<0.03). Furthermore, these three patients did not receive steroid therapy as opposed to 14/15 patients who were treated with steroids and eventually contracted CMV pneumonia post-engraftment (p<0.01). The three patients did not have two additional risk factors known for the development of CMV pneumonia: increasing age and a diagnosis of acute myeloblastic leukemia (AML) as the primary disease. Despite prompt diagnosis and therapy with ganciclovir and high doses of intravenous immunoglobulin (IVIG), two of the patients died. Tcell depleted BMT may be a risk factor for development of CMV pneumonia occurring prior to engraftment. In the era of post-BMT anti CMV prophylaxis, one should be aware that life-threatening CMV pneumonia may appear prior to engraftment and consider aggressive CMV prophylaxis.     

CHANGES OF SERUM ALPHA FETOPROTEIN BEFORE AND AFTER RADIOIMMUNOTHERAPY IN PATIENTS WITH HEPATOCELLULAR CARCINOMA

ＣＨＡＮＧＥＳＯＦＳＥＲＵＭＡＬＰＨＡＦＥＴＯＰＲＯＴＥＩＮＢＥＦＯＲＥＡＮＤＡＦＴＥＲＲＡＤＩＯＩＭＭＵＮＯＴＨＥＲＡＰＹＩＮＰＡＴＩＥＮＴＳＷＩＴＨＨＥＰＡＴＯＣＥＬＬＵＬＡＲＣＡＲＣＩＮＯＭＡＺｅｎｇＺｈａｏｃｈｏｎｇ;曾昭冲;ＴａｎｇＺｈａｏ...     

SYNERGISM BETWEEN PHOTODYNAMIC THERAPY OF ALUMINUM SULFONATED PHTHALOCYANINE AND HYPERTHERMIA ON MOUSE HEPATOMA IN VIVO AND I

ＳＹＮＥＲＧＩＳＭＢＥＴＷＥＥＮＰＨＯＴＯＤＹＮＡＭＩＣＴＨＥＲＡＰＹＯＦＡＬＵＭＩＮＵＭＳＵＬＦＯＮＡＴＥＤＰＨＴＨＡＬＯＣＹＡＮＩＮＥＡＮＤＨＹＰＥＲＴＨＥＲＭＩＡＯＮＭＯＵＳＥＨＥＰＡＴＯＭＡＩＮＶＩＶＯＡＮＤＩＮＶＩＴＲＯＦｕＭａｉｗｕ;傅乃...     

Vestibular presentation in platybasia

The platybasia consists of an upward bulge or invagination of the occiput near the foramen magnum. These patients mostly presents with neurological symptoms. The present case is a rare variant presenting with vestibular disorders only.     

Therapeutical effect of activated human macrophages on a human tumor line growing in nude mice

Human peritoneal macrophages were collected from dialysis bags of renal patients on Continuous Ambulatory Peritoneal Dialysis (CAPD), during an inflammation-free period. The macrophage suspension was cultured in presence of bacterial lipopolysaccharide (LPS) and phorbol myristate acetate (TPA). The cultured macrophages were tested for therapeutic effectiveness against a human tumor-cell fine, RC43, implanted subcutaneously in NMRI nude mice. The macrophages were injected around the tumor starting from the 14th day after inoculation, when the tumor growth was already detectable (mean tumor size 7 mm). Three injections of macrophages on days 14, 18 and 21 induced hemorrhagic patches at the tumor site and almost complete regression of the tumor. One injection of macrophages cultured either in presence of LPS + TPA or of LPS + TPA + PGE2 resulted in marked slow-down of the tumor growth. Injection of either TNF-alpha (4000 U/mouse) or of PGE2 (150 ng/mouse) given at the site of the palpable small tumor had no effect. Macrophages cultured in medium or in medium supplied with either TPA, LPS or TPA + LPS, were not effective in nude mice bearing large (16 to 19 mm) tumors. The results obtained suggest that activated human macrophages might be therapeutically effective at certain stages of human cancer.     

Carboplatin-liposomes (CPL) in immunodeficient mice: Improved antitumor activity for breast carcinomas and stimulation of hematopoiesis

Carboplatin-liposomes (CPL) have been shown to possess a strong stimulatory activity on the hematopoiesis in immunocompetent mice. As we were interested in studying this pharmacological characteristic in parallel with any antitumour effects which might be expected for the encapsulated cytostatic, we used a panel of six human breast carcinomas xenotransplanted to nude mice. The antitumor activity as well as the hematopoietic effects of the vesicles were studied in comparison to, and in combination with, the free drug. Carboplatin was encapsulated into reverse phase evaporation vesicles (REV) and injected i.p. as a single dose of 75 mg kg^-1 into tumor-free and breast-carcinomabearing animals, respectively. Following a single application of CPL in nude mice, a significant increase of the WBC numbers to about three times for that of the normal level could be observed over a period of at least 28 days. The elevation was due to an increase in both circulating granulocytes and lymphocytes. The peripheral effect was accompanied by a relative decrease of spleen cellularity, while the number of bone marrow cells was hardly affected. There was no influence detectable on circulating blood cells in SCID mice. However, a rather high toxicity of CPL for this immunodeficient mouse strain was noticed. In the panel of breast carcinomas used, free carboplatin and CPL displayed a different pattern of therapeutic efficiencies. In four of the five tumor models tested, a combination of the free with the liposomal drug showed a significant inhibition of tumor growth while effectively preventing a drug-induced ieukopenia. As a result, CPL may be recommended in tumor therapy protocols for future clinical trials in order to relieve a cytostatic-induced bone marrow suppression.     

Expression of gap junction protein Cx43 in cultured human normal and malignant lung cells

Gap junctional intercellular communication-exchange of small molecules and ions between contiguous cells through membranous gap junctional channels-is essential for growth control and tissue homeostasis. This work concerns the functional expression of gap junction protein connexin 43 (Cx43) in normal human lung cells and the changes in lung carcinoma cells. By using Northern blot hybridization analysis and Cx43 immunocytochemical methods, it was observed that cultured normal human embryonic lung cells expressed a high level of Cx43 in both mRNA and protein levels. The Cx43 immunofluorescence was localized at cell membrane regions corresponding to the location of gap junctions. These normal lung cells were competent of intercellular communication function as detected by Lucifer yellow dye transfer. In contrast to normal cells, Cx43 mRNA and protein was not detectable in the carcinoma PG cell line. These tumor cells were defective of intercellular communication function. These results demonstrate that Cx43 is expressed in normal cultured human embryonic lung cells but not in lung tumor cells. The lack of intercellular communication in the lung tumor cell line correlates with dysfunctional intercellular communication. The suggestive role of Cx as a tumor suppersor gene is discussed.     

Localization of type I and type III procollagen mrnas in breast scirrhous carcinoma byin situ hybridization

Scirrhous carcinoma is characterized by remarkable amount of collagen fibrils, mainly type I and type III collagens. The origin of collagens is still under debate. cDNA fragments of type I and type III procollagens were subcloned into Gemini pGEM vectors to synthesize the³⁵S-labeled cRNA probes. Byin situ hybridization, we have found the fibroblasts surrounding the tumor cells and cords contained abundent type I and type III procollagen mRNAs which decreased with the distance of fibroblasts from the tumor cells. In all freshly prepared tissues, the tumor cells also contained significant pro α1 (I) and pro al (III) mRNAs, but no or little pro α2(I) mRNA. The results indicated that type I and type III collagens in human scirrhous carcinoma of breast are mainly produced by fibroblasts. Tumor cells also perticipate in the disposition of collagen fibrils, probably type I trimer and type III collagens in accordance with what was observed in biochemical analysis and cell culture studies.     

Proboscis Lateralis with congenital cleft palate

Proboscis Lateralis is a congenital malformation in the form of a tubular nostril arising from the inner canthus of the eye. In some cases, proboscis is the only congenital malformation while in the rest, it may be associated with nasal, ophthalamic, oral or intracranial abnormalities. A rare case of Proboscis Lateralis with Congenital Cleft Palate is presented     

Lipoproteins in hypoxic tumor cells as traps of free radicals

We examined the role of main cell protective mechanisms in retaining the high resistance of ascitic cells (EAC, ZAH) to lipid peroxidation with respect to different stages of tumor-organism metabolic interactions. The following mechanisms were studied: (1) the activity of main EAC enzymatic antioxidants (GSH-Px, SOD); (2) changes in lipid metabolism, especially the content of the main PUFA (llnoleic and arachidonic fatty acids) in EAC cells; (3) comparison of intracellnlar resistance between EAC/ZAH cytoplasmic sections (containing LP-granules or not) to lipid peroxidation (initialized directly by UV-light). We found that the high resistance to lipid peroxidation was typical for cytoplasma sections (without LP-grannles) on all stages of tumor development in vivo. The intracellular LP-granules become the main sensitive targets for FR-action, but only in the chronic hypoxia state of EAC/ZAH tumor cells. The latter effect developed in close correlation with the following metabolic interactions: (1) increasing the proportion of PUFA (especially, arachidonic and linoleic acids) transported to EAC tumor cells from host organs and accumulated mainly in ttunor LPgranules, and (2) decreasing the cα-tocopherol content of these hypoxic EAC cells while no activation of the main cell antioxidative enzymes (GSH-Px, SOD) took place. The vitality and high resistance of EAC stationary cells were accompanied by the ‘paradoxical’ state with great differences between the resistance of the intracellnlar PUFA-rich granules and other cytoplasmic sections. A similar state was found in stationary ZAH cells. The cell state is in good agreement with the Dormandy’s suggestion that PUFA-rich granules can trap reactive radical species preventing their interaction with ‘critical’ PUFA-membranes.