P2P-SIP技术以及在远程医疗系统中的应用

介绍P2P-SIP网络系统的结构及功能以及在远程医疗系统中的应用。P2P-SIP技术充分利用了SIP协议和P2P技术各自的特点,解决了传统远程医疗系统的实时性和拓展性问题,具有很好的应用前景。     

基于H．264高清晰手术直播系统的设计与实现

针对外科手术直播中视频清晰度高、音视频实时同步的要求,本文设计实现了一套手术直播系统。该系统以H.264视频编解码标准为基础,建立了能同时对多路音视频进行高清编码传输的手术音视频控制整合单元。在实际的应用中,该系统表现出细节再现逼真、色彩还原能力强、图像延迟低、网络带宽占用低和数据易于存储维护等特点。本系统还支持异地远程视频直播和点播,在医院手术教学、远程会诊、学术会议等领域具有独特的应用优势。     

矩阵式手术直播系统接入远程医学信息网的实现

目的:探讨手术直播教学演示系统进行远程通信的开展和深化应用。方法:通过手术室的摄像机和话筒等设备采集多路视频和声音信号,通过矩阵处理和控制后接入远程医学信息网。结果:能够实现手术实时转播的远程通信。结论:使手术实时转播的范围扩大,提高了远程手术教学的水平。     

视频会议系统在我院手术直播中应用的探讨

本文从我院几年来运用视频会议系统进行手术直播的实践出发,探讨视频会议系统的功能、常见故障与解决、构建手术直播的拓扑结构和手术直播中应用视频会议系统的结果,为其他医院运用视频会议系统开展手术直播提供可借鉴的经验。     

卫星通信远程微创骨科手术直播

本文从本院的实际情况出发详述了进行微创骨科手术直播的要求,以及实现手术直播的技术准备和手段。从简单经济的角度介绍实现微创骨科手术直播的一种方法。     

在VPN上架设P2P的研究

P2P技术（Peer-to-Peer,简称P2P）迅速成为计算机界关注的热门话题之一,它被定义为网络中的计算机共享他们所拥有的一部分硬件资源,在此网络中的参与者既是资源（服务和内容）提供者（Server）,又是资源（服务和内容）获取者（Client）。P2P技术是因为解决了互联网模型中的弱链接模块的信息传递这个障碍,使得互联网的信息流动更加通畅才使他具备了这样强大的生命力和应用前景。文章就VPN上架设P2P提出一些自己的观点,供大家参考。     

心导管介入手术直播系统与应用

本文介绍了手术直播系统的功能、原理及构成。该直播系统使手术的操作手法和进展过程一目了然,在学习研讨中可以边手术边讲解,进行全方位的互动交流。     

基于P2P体系架构的医疗设备档案管理系统的构建

目的:基于P2P体系架构拟构建医疗设备档案管理系统,助力用户克服处理海量数据时出现的文件传输瓶颈问题,实现档案数据科学管理及高效分析.方法:采用云存储技术,结合P2P网络的技术架构,在此基础上设计自顶而下的双层结构体系.结合Spring Cloud微服务管理框架构建医疗设备档案管理系统.将基于P2P体系架构的医疗设备档...     

远程医学信息网进行手术直播的实现

研究远程医学信息网和手术直播系统的整合技术,实现远程手术直播。通过手术室摄像机和话筒采集视频和声音信号,经矩阵主机处理后接入远程医学信息网,实现了手术实时转播,实现了远程收看,从而达到远程教育多样化,提高远程教学水平的目的。     

血E_2、P、LH联合阴道B超、尿LH峰监测排卵对IUI手术时机的影响

目的:分析夫精宫腔内人工授精(Intrauterine insemination,IUI)患者的临床资料,探讨IUI过程中血雌二醇(E2)、孕酮(P)、黄体生成素(LH)联合阴超以及尿LH峰监测排卵对IUI手术时机的影响。方法:选择2009年1月～2011月12月因不孕在广东医学院附属医院生殖医学中心进行IUI治疗的夫妇89对共190个周期,将进入周期的患者随机分为阴道彩超+尿LH峰监测卵泡发育组(A组)和阴道彩超+尿LH峰+血E2、P、LH监测卵泡发育组(B组),比较两组临床妊娠率的差异。结果:B组临床妊娠率高于A组,差异有统计学意义(P<0.05)。结论:IUI过程中血E2、P、LH联合阴超、尿LH峰监测排卵对IUI手术时机的判断优于仅阴超+尿LH峰监测排卵。     

CerbB_2和P~(53)在子宫内膜癌中的表达及临床意义

目的探讨CerbB2和P53在子宫内膜癌中的表达的意义以及二者间的关系。方法应用免疫组织化学法回顾性分析了85例子宫内膜癌患者的石蜡标本中CerbB2和P53蛋白的表达,并对其生存期进行随访。结果CerbB2和P53表达的阳性率分别为27.06%、45.88%;CerbB2阳性表达者5年生存率为52.38%,阴性表达者为73.65%,差异有显著意义(P<0.05);P53阳性表达者5年生存率为59.25%,阴性表达者为84.36%,差异有显著意义(P<0.05);P53,CerbB2和P53表达间无相关性。结论CerbB2和P53在子宫内膜癌中的表达与内膜癌的生物学行为有关,可作为子宫内膜癌预后的指标,指导子宫内膜癌术后的治疗。     

New monoclonal antibodies specific for mammalian protamines P1 and P2

ABSTRACT

The expression of protamines and the binding of these small arginine-rich proteins to DNA complete the process of spermatid chromatin reorganization and the global inactivation of the male’s haploid genome that occurs during the final stages of sperm development in mammals. While a number of anti-protamine antibodies have been created during the last 40 years, only a few have proven useful for detecting the presence of the protamines, determining the timing of their expression and deposition in chromatin, and investigating their structure and function in both maturing spermatids and sperm. The aim of this effort was to develop an additional set of monoclonal antibodies (MAbs) that not only recognize new P1 and P2 protamine epitopes but also work well as IHC reagents for detecting and identifying mammalian protamines in testicular tissue and ejaculated sperm. Using a combination of native and synthetic human protamines as antigens, 38 hybridoma clones recognizing human protamine P1 or P2 were generated. Antibodies produced by the 12 best clones were screened for selectivity by enzyme-linked immunosorbent assay, and two were found to recognize only human protamine P1 or P2, while a number of the others bound to both the human and mouse proteins. One MAb recognized every protamine tested. All the antibodies, including one recognizing stallion P1 and another recognizing stallion P2, bound to the native protamines in the chromatin of spermatids or sperm. While the majority labeled only elongating spermatids or sperm, several of the antibodies were found to also bind to the cytoplasm or nuclei of cells that lack protamine, which indicates these MAbs must recognize epitopes present in the protamines that are also found in other proteins. Thirteen overlapping human protamine P1 peptides were synthesized and subsequently used to identify the epitopes recognized by the six best antibodies.

Abbreviations: BSA: bovine serum albumin; ELISA: enzyme-linked immunosorbent assay; HCl: hydrochloric acid; IHC: immunohistochemistry; i.p: intraperitoneal; LIS: lithium diiodosalicylate; MAb: monoclonal antibody; PBS: phosphate buffered saline     

Structure-activity relationships of novel P2-receptor antagonists structurally related to Reactive Blue 2

P2 membrane receptors for nucleotides represent significant targets for experimental pharmacology and drug research. In earlier publications, we have shown that Reactive Blue 2 (RB 2), one of the most widely used P2-receptor antagonists, displays only moderate affinity and does not discriminate between native P2X- and P2Y-receptor subtypes. In the present study we have pharmacologically evaluated a series of 15 synthesized and re-evaluated four commercially obtained and chromatographically purified RB 2 type anthraquinone derivatives on contractions of the rat vas deferens (RVD) elicited by alpha,beta-methylene ATP (alpha,beta-meATP), mediated by P2X1-receptors, and relaxations of the carbachol-precontracted guinea-pig taenia coli (GPTC) elicited by adenosine 5'-O-(2-thiodiphosphate) (ADPbetaS), mediated by P2Y1-like receptors. Based on the structure-activity relationships (SAR) it is concluded that hydrophobic interactions of aromatic pi-electron systems, hydrogen bonds with nitrogen as donor and acceptor atoms, and, particularly, position, conformational distance and number of anionic sulfonate groups are of great importance for the blockade of the two native P2-receptor subtypes. We have also identified novel, for the most part reversible antagonists that bind with higher affinity and improved subtype selectivity in comparison to RB 2. In particular, 1-amino-4-{4-[4-chloro-6-(2-sulfonatophenylamino)-[1,3,5]triazine-2-ylamino]-2-sulfonatophenylamino}-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid trisodium salt (MG 50-3-1) is the most potent antagonist at the P2Y1-like-receptors of the GPTC reported so far (IC50=4.6 nM). It is significantly less potent as reversible antagonist at the P2X1-receptors of the RVD (IC50=2.8 microM). Thus, MG 50-3-1 represents a selective pharmacological tool and may be a lead compound for future investigations.     

上皮性卵巢癌中Skp2和P27蛋白的表达及临床意义

目的:探讨Skp2和P27蛋白表达与上皮性卵巢癌发生、发展及预后评价的意义。方法:采用免疫组化法检测15例卵巢上皮性良性肿瘤、10例卵巢上皮性交界性肿瘤及58例上皮性卵巢癌中Skp2和P27蛋白的表达情况。结果:Skp2在卵巢上皮性良性及交界性肿瘤中的表达均为阴性,而在上皮性卵巢癌中高表达,阳性率为48.28%(28/58),Skp2在中低分化组、Ⅲ/Ⅳ期组及有淋巴结转移组分别高于高分化组(P<0.01)、Ⅰ/Ⅱ期组(P<0.01)及无淋巴结转移组(P<0.01)。在不同年龄及组织类型的卵巢癌中表达的差异无统计学意义。P27蛋白与Skp2蛋白在卵巢癌的表达呈负相关。结论:Skp2和P27蛋白在上皮性卵巢癌的发生、发展及预后中起重要作用,对卵巢癌的预后有意义。     

P2X3受体介导的痛觉传递及其作用机制

目前,国际上认为疼痛是继呼吸、脉搏、血压、体温之后的第五个生命体征,为生命体征的重要指标。P2X3受体是ATP—门控性离子通道家族的一个亚型,在慢性疼痛的伤害性信息传递中起重要作用,由于P2X3受体的分布仅限于感觉神经元,对该通道的处理就可能提供一个镇痛作用既强又无副作用的慢性疼痛的治疗方法。