老年高血压合并射血分数保留的心衰患者血浆BNP、同型半胱氨酸、C反应蛋白水平变化的关系及意义

目的 观察HF-PEF患者血浆BNP、HCY、CRP水平变化,并探讨其临床意义.方法 选取HF-PEF患者108例和健康体检者81例为研究对象,比较两组患者SBP、DBP、BNP、HCY、CRP差异及不同NYHA分级间LVEF、BNP、HCY、CRP差异.结果 与健康对照组比较,HF-PEF组SBP、DBP、BNP、HCY、CRP均显著升高,差异具有统计学意义(P＜0.05).NYHA分级3组患者的LVEF和血浆BNP、HCY、CRP水平比较有显著性差异,差异有统计学意义(F值分别为7.29、21.23、8.13、6.94,P值均＜0.05);与NYHA分级Ⅱ级比较,NYHA分级Ⅲ级、Ⅳ级LVEF显著降低,BNP、HCY、CRP显著升高,差异均具有统计学意义(P＜0.05);与NYHA分级Ⅲ级比较,NYHA分级Ⅳ级LVEF显著降低,BNP、HCY、CRP显著升高,差异均具有统计学意义(P＜0.05).Pearson相关性分析结果显示,血浆HCY与BNP、CRP呈显著正相关(r=0.764,r =0.535;P ＜0.05);血浆BNP与CRP无显著性相关(r=0.226,P＞0.05).结果 HF-PEF患者血浆HCY、BNP、CRP水平显著升高,且随HF-PEF病情的严重程度呈逐渐升高趋势.     

血清真胰岛素、脂联素、同型半胱氨酸、高敏C反应蛋白水平变化与发生冠心病的相关性分析

通过对冠心病(CHD)发生机理和危险因素的研究,探讨新的CHD标志物检测对CHD的发生、发展、诊断、治疗的价值.应用电化学发光免疫、酶联免疫和免疫放射分析的方法,对185例冠心病患者及其中的32例不稳定型心绞痛(UAP)患者经皮经腔冠状动脉成形术(PTCA)/支架术治疗前与治疗后二周和30例正常对照者血清中真胰岛素(TI)、脂联素(APN)、同型半胱氨酸(HCY)、高敏C-反应蛋白(H-CRP)水平进行检测,应用统计学方法对其各组及治疗组治疗前后的水平和比值进行对比和相关性分析.结果显示,APN、TI、HCY、H-CRP冠心病各组与正常对照组比较有显著性差异(P＜0.01),UAP组与稳定性心绞痛(SAP)组比较有显著性差异(P＜0.01),但APN水平明显低于正常对照组,32例UAP患者经PTCA/支架术治疗前后比较,4项指标及TI/APN、HCY/CRP比值均有显著性差异(P＜0.001),除APN升高外,其余指标均明显降低.结论:APN、TI、HCY、H-CRP水平变化与CHD发生、发展密切相关,是发生CHD的重要的危险因素,对CHD的诊断或(和)分型诊断、治疗、预后判断具有重要价值.     

GDF15、BNP在慢性心力衰竭患者血清中的表达及临床意义

目的:探讨慢性心力衰竭患者血清生长分化因子15(growth differentiation factor 15,GDF15)水平与NYHA心功能分级、血浆脑钠肽(brain natriuretic peptide,BNP)相关性及临床意义.方法:慢性心力衰竭患者80例,对照组30例,采用酶联免疫吸附法(ELISA)测定血清GDF15水平,微粒子酶免疫分析(MEIA)检测血浆BNP水平,NYHA心功能分级标准对慢性心力衰竭组进行分级.比较慢性心力衰竭组与对照组血清GDF15浓度的变化,分析GDF15与BNP的相关性,了解GDF15与NYHA心功能分级之间的相关性.结果:慢性心力衰竭组血清GDF15水平(2840.85±846.18 ng/l)和对照组(807.33±355.56 ng/l)比较,差异有统计学意义(P＜0.01);NYHA心功能分级不同亚组GDF15水平具有明显差别(P＜0.01).慢性心力衰竭组血清GDF15与血浆BNP水平呈显著正相关(r=0.798,P＜0.05),GDF15水平随BNP水平的升高而升高;与NYHA心功能分级正相关(r=0.840,P=0.000),随NYHA心功能分级的增高而升高.结论:血清GDF15可能作为慢性心力衰竭患者一个新的生物标志物,可以客观评价心力衰竭的严重程度,预测患者的活动耐量.血清GDF15浓度与血浆BNP、NYHA心功能分级呈正相关,为综合评价心力衰竭患者心功能状态提供了一个新的思路.     

子痫患者血清TNF-α、IL-6、 CRP、 Lp-PLA2、PON-1和APN水平的变化及临床研究

目的 探讨肿瘤坏死因子(TNF-α)、白介素6(IL-6)、C反应蛋白(CRP)、血清脂蛋白相关磷脂酶A2(Lp-PLA2)、对氧磷酶1(PON-1)以及脂联素(APN)在子痫前期孕妇血清中的变化及其临床意义.方法 选择2013年8月至2016年6月我院产科进行检测的孕妇160例,其中,妊娠期高血压孕妇(GH),轻度子痫前期孕妇(MPE)、重度子痫前期孕妇(SPE)和正常妊娠期孕妇(NC)各40例.采用酶联免疫吸附法检测IL-6、TNF-α以及Lp-PLA2水平,采用乳胶增强透射免疫比浊法检测血清CRP含量,利用qRT-PCR法检测PON-1和APN mRNA表达水平,采用免疫印迹法检测PON-1和APN蛋白表达水平,并比较各组之间的差异.结果 子痫前期孕妇血清TNF-a、IL-6、CRP以及Lp-PLA2水平明显高于正常妊娠对照组及妊娠期高血压组,差异存在统计学意义(P＜0.05).重度子痫前期患者血清TNF-a、IL-6、CRP以及Lp-PLA2水平明显高于轻度子痫前期患者,差异存在统计学意义(P＜0.05).子痫前期孕妇血清PON-1和APN mRNA和蛋白表达水平明显低于正常妊娠对照组及妊娠期高血压组,差异存在统计学意义(P＜0.05),且重度子痫前期患者血清PON-1和APNmRNA和蛋白水平明显低于轻度子痫前期患者,差异存在统计学意义(P＜0.05).结论 子痫前期患者血清中TNF-a、IL-6 、CRP以及Lp-PLA2水平的升高,PON-1和APN水平的降低可能参与子痫前期的发生.     

急性心肌梗死患者Mb,TnI,BNP和HCY水平变化与临床分析

目的 :为观察急性心肌梗死 (AMI)患者血清中肌红蛋白 (Mb)、肌钙蛋白I(TnI)、脑钠肽 (BNP)、同型半胱氨酸 (HCY)水平动态变化和AMI治疗前后的水平变化 ,并探讨AMI发病机制 ,为诊断、治疗及预后判断提供依据。方法 :应用化学发光、酶联免疫分析的方法 ,对 5 4例AMI患者治疗前后和 30名对照者血清中的Mb、BNP、TnI和HCY水平进行检测。结果 :AMI患者四项指标明显高于对照组 (P <0 0 1) ;AMI患者经溶栓治疗后 ,血清中四项指标明显下降 ,与治疗前比较有显著性差异 (P <0 0 1) ;在AMI治疗前后BNP与Mb水平变化呈正相关 (r1=0 .874 ,r2 =0 .5 14 ) ,TnI与HCY水平变化呈正相关 (r3 =0 .75 1,r4=0 .4 6 8) ;但AMI经溶栓治疗后Mb水平恢复至正常水平 (P >0 0 5 ) ,而BNP、TnI和HCY水平虽然下降明显 ,但与对照组比较仍有明显差异 (P <0 0 5 ) ;在其动态变化提示 ,AMI早期以检测HCY、TnI为佳 ,中期以检测Mb、BNP为佳 ,末期以TnI、BNP检测为佳。结论 :AMI患者血清中Mb、BNP、TnI和HCY水平的动态变化说明参与了AMI的发生、发展 ,并提示四项指标在AMI患者发病过程中不同阶段有其不同的诊疗价值 ,从而为AMI的诊断、治疗、预后判断提供了新的理论依据。     

冠心病患者血清中hs-CRP、APN和BNP的表达及意义研究

目的 研究超敏C反应蛋白(hs-CRP)、脂联素(APN)和脑尿钠肽(BNP)在冠心病患者中的表达水平及临床价值.方法 选择我院2014年1月至2016年9月心血管内科收治的154例冠心病患者(观察组)及105例同期健康体检人群(对照组)作为研究对象.检测观察组和对照组受试对象血清中hs-CRP、APN和BNP的表达水平,通过Logistic回归分析冠心病发病的危险因素并运用ROC曲线分析hs-CRP、APN、BNP及联合检测在冠心病中的诊断效能.结果 ①观察组患者血清中hs-CRP、BNP表达高于对照组,血清APN表达低于对照组,差异均具有统计学意义(P＜0.05).②冠心病患者GRACE评分与hs-CRP、BNP呈正相关,与APN呈负相关.③Logistic回归分析得出,hs-CRP、BNP和APN均与冠心病发病有关.④联合检测在冠心病中的诊断效能明显高于hs-CRP、APN和BNP.结论 冠心病患者血清hs-CRP、BNP高表达,APN低表达,其与冠心病严重程度相关,可辅助诊断冠心病.     

血浆HCY水平与高血压患者血压分级及早期肾功能损伤的关系分析

目的 分析血浆同型半胱氨酸(HCY)水平与高血压患者血压分级及早期肾功能损伤的关系,以指导临床早期防治.方法 纳入2017年1月至2017年7月期间我院门诊及住院的200例高血压患者为研究对象,根据血压分级标准分组:Ⅰ级组(n=60)、Ⅱ级组(n=84)、Ⅲ级组(n=56),根据是否符合原发性高血压早期肾功能损害诊断标准分组:肾功能损害组(n=58)、无肾功能损害组(n=142);另选同期体检的健康志愿者80例作为对照组.比较血压分级各组血压水平、血浆HCY水平及肾功能指标,进一步分析HCY与血压、肾功能指标的关系.结果 Ⅰ级组、Ⅱ级组、Ⅲ级组早期肾功能损伤发生率为5.00％、17.86％、71.43％,差异有统计学意义(P＜0.05).Ⅰ级组、Ⅱ级组、Ⅲ级组患者收缩压、舒张压及血浆HCY水平显著高于对照组,差异有统计学意义(P＜0.05),且Ⅰ级组、Ⅱ级组、Ⅲ级组三组之间收缩压、舒张压及血浆HCY水平比较差异有统计学意义(P＜0.05).无肾功能损害组、肾功能损伤组患者血清胱抑素C(CysC)、肌酐(SCr)及尿酸(UA),尿微量白蛋白(mALB),血浆HCY水平显著高于对照组,差异有统计学意义(P＜0.05),且早期肾功能损伤组患者血清CysC、SCr、UA,尿液mALB,血浆HCY水平显著高于无肾功能损害组(P＜0.05).高血压患者血浆HCY水平与舒张压、收缩压水平呈中度正相关(r=0.51,0.54,P＜0.05),与血清CysC、SCr、UA及尿液mALB水平呈高度正相关(r=0.80,0.82,0.81,0.86,P＜0.05).结论 高血压患者发生早期肾脏损伤的风险较高,血浆HCY水平与高血压患者血压分级、早期肾功能损伤显著相关,血浆HCY水平检测可作为高血压病情程度及早期肾功能损伤的诊断指标.     

原发性高血压合并糖尿病患者血清APN和INS的变化及意义

目的:探讨原发性高血压(PH)合并糖尿病(DM)患者血清脂联素(APN)和胰岛素(INS)变化及其临床意义.方法:采用放射免疫分析测定了126例PH合并DM患者、92例PH患者和40例健康对照者的血清APN和INS水平,并将结果进行分析.结果:PH合并DM组血清APN水平显著低于PH组(P<0.01)和健康对照组比较(P<0.01),INS水平显著高于健康对照组(P<0.01),显著低于PH组(P<0.05);PH组血清APN显著低于健康对照组(P<0.01),INS水平显著高于健康对照组(P<0.01).在PH合并DM组中,APN与INS两者间呈显著负相关(r=-0.496,P<0.01),APN与平均收缩压(SBP)(r=0.586,P<0.01)、平均舒张压(DBP)(r=0.416,P<0.05)呈显著负相关,INS与SBP(r=0.433,P<0.05)、DBP(r=0.401,P<0.05)呈显著正相关.结论:PH合并DM患者血清APN水平显著降低,INS水平显著高于健康对照组,却显著低于PH组;APN与INS相互间呈负相关.     

妊娠期糖尿病患者血清游离脂肪酸与胱抑素C的相关性研究

目的 探讨妊娠期糖尿病患者游离脂肪酸(FFA)和胱抑素C(CysC)相关性及临床价值.方法 选取妊娠期糖尿病患者85例,另选取健康对照组67例;检测血清FFA(酶法)和CysC(免疫透射比浊法),对检测结果进行统计学分析.结果 妊娠期糖尿病患者血清FFA和CysC均有升高,与对照组比较具有统计学意义(P＜0.05);妊娠期糖尿病患者血清FFA水平和CysC水平呈正相关(r=0.508,t=5.902,P＜0.01).结论 妊娠期糖尿病患者血清FFA和CysC均有升高,血清FFA水平和CysC水平呈正相关,联合检测血清FFA和CysC水平有助于妊娠期糖尿的诊断与病情评估.     

原发性高血压肾病患者血浆ET-1和血清APN、CysC检测的临床意义

目的:探讨原发性高血压肾病患者血浆ET-1和血清APN、CysC水平的变化及临床意义。方法:应用放射免疫分析和酶联法对32例原发性高血压肾病患者进行血浆ET-1和血清APN、CysC检测,并同时与35名正常人作比较。结果:原发性高血压肾病患者血浆ET-1和血清APN、CysC水平显著地高于正常人组(P<0.01),而血清APN水平又非常显著地低于正常人组(P<0.01),血浆ET-1水平与CysC水平呈正相关(r=0.6228,P<0.01),与APN水平呈显著负相关(r=-0.4012,P<0.01)。结论:检测原发性高血压肾病患者血浆ET-1和血清CysC、APN水平的变化,两者均具较高检测率。因此,有助于对疾病进程的评估,包括发病机制、预防和指导用药方针。     

Schizophrenia in a North Swedish geographical isolate, 1900–1977. Epidemiology, genetics and biochemistry

Over 200 schizophrenic patients belonging to three major and interrelated pedigree complexes have been investigated over the past 30 years in a North Swedish geographically isolated population, presently numbering about 6,000. An intensive investigation of a number of biochemical correlates and genetic markers in a few selected families belonging to one of the major pedigrees has indicated new strategies for the current research program.
Schizophrenia, as defined operationally, is significantly associated with decreased activities of two enzymes (1) blood platelet monoamine oxidase, (2) plasma dopamine-β-hydroxylase, and (3) with the genetic marker Gc² (group specific antigen). Both enzymes are subject to genetic variation. A positive score for linkage between schizophrenia and low plasma DBH activity has been calculated, but, so far, available data are insufficient for discrimination between linkage and partial contribution of genetically controlled low plasma DBH to the pathogenesis of the disease. Alternatively, both mechanisms could be involved.
As a model for continued research, schizophrenia is explained as based on a double dominant-recessive genotype (Aabb), representing a vulnerability which in about 50 % of cases develops into clinical schizophrenia. It is suggested that the dominant mutation (A) operates on or affects MAO activity, and that the recessive genotype (bb) is instrumental in low variates of DBH activity and very likely such variates within the normal range of physiological variation. Moreover, it is suggested that the combined effects of MAO- and DBH-reduced efficiency on the metabolism of e.g. dopamine could be an essential pathogenic mechanism for the schizophrenic illness which is segregating in this population.     

HLA in North Colombia Chimila Amerindians

HLA-A,-B,-C,-DRB1 and -DQB1 alleles have been studied in Chimila Amerindians from Sabana de San Angel (North Colombian Coast) by using high resolution molecular typing. A frequent extended haplotype was found:HLA-A*24:02-B*51:10-C*15:02-BRB1*04:07-DQB1*03:02 (28.7%) which has also been described in Amerinndian Mayos Mexican population (Mexico, California Gulf, Pacific Ocean). Other haplotypes had already been found in Amerindians from Mexico (Pacific and Atlantic Coast), Peru (highlands and Amazon Basin), Bolivia and North USA. A geographic pattern according to HLA allele or haplotype frequencies is lacking in Amerindians, as already known. Also, five new extended haplotypes were found in Chimila Amerindians. Their HLA-A*24:02 high frequencies characteristic is shared with aboriginal populations of Taiwan; also, HLA-C*01:02 high frequencies are found in New Zealand Maoris, New Caledonians and Kimberly Aborigines from Australia. Finally, this study may show a model of evolutionary factors acting and rising one HLA allele frequency (-A*24:02), but not in others that belong to the same or different HLA loci.     

The universal muscular chamber. A basic unit of the genitourinary, cardiovascular, gastro-intestinal, skeletal, cutaneous, respiratory and other systems, endocameral hypertension; and spasm, the key to their idiopathic diseases and arthropathies

Starting with the integument, we see many organs are contractile sacs or multiples thereof, which tubes or bags constitute the major part of the entire body. Recognition of this basic unit and its characteristics sheds new light, individually and collectively, on many disorders previously considered unrelated. Muscular tears and perforations develop in the walls of these chambers, being no way peculiar to those organs, wherein, hydrochloric acid occurs. So, it is not necessary to explain the absence of excessive acid from patients who exhibit holes in the gastric, uterine, aortic, duodenal, rectal, pulmonary, retina, and other walls. Muscle, not acid is the great common factor relating idiopathic disorders in the gastrointestinal tract to each other and to similar diseases in other systems. When the units are linked together, the lesions tend to appear as arthropathies, i.e. at the joints. Rephrasing common-place observations, frees us from conventional, conceptual cul-de-sacs. An observation is only as good as its interpretation, so all possibilities must be considered, otherwise, we will remain blinded by our misconceptions.     

Der Einfluß von Nahrungsmitteln und Rauchen auf die klinisch-chemische Diagnostik von Phäochromozytom,Neuroblastom und Karzinoid-Syndrom

Zusammenfassung Der Einfluß von verschiedenen Nahrungsmitteln auf Methoden zur Bestimmung von Adrenalin (AD), Noradrenalin (NA), Vanillinmandelsäure (VMS), Metanephrinen (MN), Homovanillinsäure (HVS) und 5-Hydroxyindolessigsäure (5-HIE) im 24 h-Harn zur Diagnose des Phäochromozytoms bzw. Karzinoid-Syndroms wurde untersucht. Die in die Untersuchung einbezogenen Nahrungsmittel waren: Tee, Kaffee, Mandeln, Ananas, Käse, Walnüsse, Vanillepudding, Bananen, Tomaten und Milchschokolade. Außerdem wurde der Einfluß des Zigarettenrauchens auf die Bestimmung von AD, NA, VMS und MN untersucht.Walnüsse führten zu einer starken Erhöhung der 5-HIE-Ausscheidung. Bananen erhöhten die Ausscheidung von AD, NA, VMS, MN und 5-HIE. Kaffee und Ananas bewirkten eine geringe Zunahme der MN-Werte. Rauchen von 20–30 Zigaretten/Tag beeinflußte keine der vier Variablen.Wenn die beschriebenen Methoden benutzt werden, sollte lediglich auf den Verzehr von Bananen und Walnüssen vor und während der Harnsammelperioden verzichtet werden, da die oberen Normgrenzen im Harn überschritten werden könnten. Ein Verzicht auf Kaffee und Ananas in normalen Mengen ist nicht erforderlich. Es besteht kein Anlaß, weiterhin die bisherigen umfangreichen Restriktionen der übrigen Nahrungsmittel beizubehalten.     

Renal dysplasia and asplenia in two sibs

A family is reported in which two sibs, one male and the other female, both died within 24 hours of birth with enlarged polycystic kidneys. Postmortem histology in the second child showed gross renal dysplasia. In both children the pancreas was enlarged, nodular and cystic but the liver appeared macroscopically normal. In the second child, histological examination confirmed pancreatic fibrosis with cystic dilation of ducts, but showed portal fibrosis with bile duct proliferation in the liver.
This combination of findings is very reminiscent of those in a girl and her brother reported by Ivemark et al. (1959). The children reported here also showed absence or hypoplasia of the spleen, cardiac anomalies and other features of the Ivemark syndrome (Ivemark 1955), a quite different, usually sporadic, congenital disorder. It is suggested that the children described here have a distinct lethal congenital disorder, probably inherited in an autosomal recessive manner.     

A comparative study of the effects of dimebon,obsidan, finoptin,and cordaron on the functional state of ischemic focus and size of necrotic zone in experimental myocardial infarction

Dimebon, an antihistamine agent, exerts a moderate antianginal effect, improving the function of ischemic focus in the myocardium and decreasing the necrotic zone in experimental myocardial infarction. Dimebon is less active than obsidan, finoptin (except for the size of the necrotic zone), and cordaron. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 122, No. 12, pp. 642–644, December, 1996     

Effects of sex steroid hormones on lipid peroxidation and glutathione antioxidant system in rat skin

Effects of estradiol and testosterone on the intensity of lipid peroxidation and contents of glutathione redox system components in the dermis and epidermis of rat skin were studied. Only estradiol induced considerable dose-dependent and tissue-specific biphasic antioxidant effects on the skin. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 128, No. 12, pp. 663–666, December, 1999     

Adhesion and growth of CaCo2 cells on surface-modified PEEK substrata

A series of surface-functionalized poly(ether ether ketone) (PEEK) films has been prepared by selective wet-chemistry; they are hydroxylated polymer (PEEK-OH) obtained by reduction, aminated polymer (PEEK-[]-NH2) prepared by coupling a diisocyanate reagent to PEEKOH (PEEK-[]-NCO) followed by hydrolysis, and carboxylated and aminocarboxylated polymers (PEEK-[]-GABA and PEEK-Lysine) resulting from the coupling of aminoacids to PEEK-[]-NCO. The aminated and carboxylated substrata promoted the adhesion and growth of CaCo2 cells in the presence of serum. Fibronectin (FN), an extra-cellular matrix protein, has been covalently fixed and/or adsorbed on various PEEK substrata, in the presence or not of a polymeric surfactant (Pluronic F68). The performances of the FN-grafted substrata (PEEK-[]-FN(1) and PEEK-[]-FN(2)) were significantly higher than those of reference substrata simply coated with FN (PEEK-OH(+FN)(1) and (2), PEEK-[]-NH2(+FN)(1) and (2)), considering the adhesion and spreading of CaCo2 cells in the absence of serum. Moreover, the stability of the adherent cells on the FN-adsorbed substrata dramatically depended on the experimental conditions applied during the PEEK coating with FN.