腹泻患者粪便弧菌属及气单胞菌属分离鉴定结果分析

作者从１３５６例腹泻患者粪便中分离出１－２株弧菌科病原菌，分离率为７．５％，其中：弧菌属８６株，分离率为６．３％，共１０个菌种；气单胞菌属１６株，分离率为１．２％，共３个菌种。均发经生化反应和血清试验证实。结果表明：弧菌属和气单胞菌属是嘉兴地区感染性腹泻的常见病原菌。     

夏秋季腹泻中气单胞菌属的分离与鉴定

目的探讨夏秋季腹泻患者中单胞菌属分离及鉴定结果。方法收集本院2012年6月－2012年10月收治的215例急性腹泻患者为研究对象,收集患者粪便样本进行单胞菌属细胞分离及鉴定。结果215份大便样本中单胞菌属阳性菌株18株,其中嗜水气单胞菌8株,温和气单胞菌6株,豚鼠气单胞菌4株。经药敏试验发现,单胞菌属对庆大霉素、卡那霉素、链霉素、丁胺卡那、四环素、新霉素、复方磺胺等药物较敏感,而对青霉素G、氨苄霉素耐药性较高。结论夏秋季腹泻患者中单胞菌属检出率较高,应引起临床及实验室的重视。     

关于气单胞菌混合感染引起食物中毒的病原学检测

目的探索引起细菌性食物中毒的病原学原因。方法采集饭店剩余食物、厨房用具及物表拭子、腹泻患者呕吐物和肛拭子样本进行鉴定及药敏试验,以生化分型方法和BD PhoenixTM-100凤凰全自动细菌鉴定系统鉴定菌株,用K-B法进行药敏试验。结果从采集的所有样本中检出3种气单胞菌,分别为温和气单胞菌、嗜水气单胞菌和豚鼠气单胞菌,其药敏结果分别为：嗜水气单胞菌对氨苄西林、头孢美唑、青霉素耐药,对其他抗生素敏感;豚鼠气单胞菌对氨苄西林、苯唑西林耐药,对其他抗生素敏感;温和气单胞菌对四环素、氨苄西林耐药,对其他抗生素敏感。结论这是一起罕见的3种气单胞菌混合感染引起的细菌性食物中毒。建议将气单胞菌属作为肠道感染性腹泻的常规致病菌,以免造成漏检,给临床诊断和治疗带来困难。     

气单胞菌基因种分离鉴定及耐药性研究

目的：探讨气单胞菌基因种与表型生化反应关系。方法：建立气单胞菌基因种鉴定方法;本实验采用被筛选的9种表型生化反应,鉴定45株气单胞菌基因种;采用MicroScan Gram Negative Panel 21微量液体稀释法报告气单胞菌基因种体外对名种抗生素的敏感性。根据NCCLs文件报告MIC、敏感、耐药。结果：本地区气单胞菌基因种主要是HG1、HG4、HG9、HG12、HG13;未见HG2、HG3、HG5、HG6、HG7、HG8、HG10、HG11。结论：气单胞菌基因种对头胞噻肟、头胞曲松、环丙沙星、替卡西林、复方新诺明敏感性为100%,对头孢噻吩耐药,对其它抗生素呈现不同程度的耐药。     

肝硬化患者气单胞菌属感染58例临床分析

气单胞菌属 (aeromonas)细菌是常见的腐物寄生菌 ,存在于土壤、下水道污物、淡水或盐水中 ,易引起免疫缺陷或免疫低下者感染。人类气单胞菌属感染主要表现为腹泻和蜂窝组织炎。我院于 2 0 0 0年 1月至 2 0 0 1年 12月 ,发现 5 8例气单胞菌属引起的自发性细菌性腹膜炎及败血症 ,现就其气单胞菌的药敏资料及抗生素治疗的疗效结果作一临床分析 ,总结报告如下。1　资料和方法1.1　资料　患者 5 8例 ,其中男性 5 2例 ,女性 6例 ,年龄31～ 6 7岁 ,平均 (46 .94± 8.6 1)岁。均为肝硬化患者 ,并发腹水 5 5例 ,原发性腹膜炎 4 8例 ,败血症 4 1例 ,慢…     

烫伤创面分泌物及血标本中分离出嗜水气单胞菌1例

烫伤创面分泌物及血标本中分离出嗜水气单胞菌１例闫智永１叶淑芳２何波１毕春霞２病人，女，３７岁。因烫伤１０ｍｉｎ入院。查体：Ｔ３８℃，颈部以下皮肤烫伤，Ⅲ度烫伤面积为５０％．实验室检查：Ｈｂ１４５ｇ／Ｌ，ＷＢＣ１４．０×１０９／Ｌ，Ｎ０．９０，Ｌ０．１...     

腹泻患者肠粘膜中气单胞菌属感染

气单胞菌属为弧菌科的一个属，由一群革兰阴性兼性厌氧的杆菌组成。由于长期以来对其在肠道感染中的致病意义重视不够，以致忽略了它的临床致病作用。近年来，此菌引起的传染性腹泻及急性胃肠炎的报告在世界各国日益增多。我们对３２例腹泻患者内镜检查时取肠粘膜培养，现...     

嗜麦芽窄食单胞菌耐药性分析

目的 使临床医生了解嗜麦芽窄食单胞菌的耐药性并选择有效抗生素治疗其所致感染。方法采用Ｋ－Ｂ纸片扩散法进行抗生素敏感试验,采用双纸片扩散法检测超广谱β－内酰胺酶（ＥＳＢＬ）。结果 嗜麦芽窄食单胞菌对大多数β－内酰胺类抗生素耐药,对青霉素类抗生素,亚胺培南,一代,二代及三代头孢菌素中头孢曲松、头孢噻肟几乎１００％耐药,在耐三代头孢类抗生素的嗜麦芽窄食单胞菌中有７７．４％的菌株产生超广谱β－内酰胺酶（Ｅ     

夏秋季腹泻便中气单胞菌属的分离与鉴定

20 0 1年 7月～ 10月采集了崇文区 2 10份急性腹泻患者粪便 ,除开展了致病性大肠杆菌 ,沙门氏菌 ,志贺氏菌 ,金黄色葡萄球菌的检测外。还开展了气单胞菌属的检测 ,共检出阳性菌株 32株。其中沙门氏菌 3株(鼠伤寒沙门氏菌 1株 ,都伯林沙门氏菌 2株 ) ,志贺氏菌 6株 (宋内氏菌 4株 ,福氏Y变种 2株 )致病性大肠杆菌 4株 ,金黄色葡萄球菌 2株。分离到气单胞菌属的细菌 14株 ,检出率为 6 .6 7% ,其中豚鼠气单胞菌 8株 ,温和气单胞菌 5株 ,嗜水气单胞菌 1株 ,结果表明夏秋季感染性腹泻气单胞菌属亦属常见 ,应引起临床及实验室的重视 .     

气单胞菌及其感染研究进展

气单胞菌在自然界分布广泛，普遍存在于淡水、污水、淤泥、土壤和人类粪便中，有致病性菌株和非致病性菌株之分。致病性菌株可感染鱼类、两栖类、爬行类、鸟类和哺乳类等动物，临床上以急性出血性败血症为主要特征。气单胞菌可引起人肠道感染和肠道外感染，如败血症、伤口、眼、骨关节、腹腔感染和其它感染，其中嗜水气单胞菌是一种典型的人-畜-鱼共患病原菌，人类可因致病性嗜水气单胞菌感染而发生腹泻、食物中毒、继发感染等等。本文对国内外有关气单胞菌的感染研究进展，综述如下。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.