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一种水稻酰基辅酶A结合蛋白cDNA的鉴定 总被引:1,自引:0,他引:1
对一水稻cDNA克隆(R1908)的分析表明,其可能编码水稻酰基辅酶A结合蛋白(acyl-CoA-binding protein,ACBP)。South-ern杂交显示水稻(Oryza sativa L)基因组中仅有一个该基因的拷贝。Northern分析表明水稻的ACBP基因在水稻的根、茎、叶、叶鞘、黄化苗和幼穗中皆表达,而以黄化苗的绿苗叶鞘中的表达高于绿苗叶片。 相似文献
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对一水稻cDNA 克隆(R1908) 的分析表明, 其可能编码水稻酰基辅酶A 结合蛋白(acylCoAbinding protein,ACBP)。Southern 杂交显示水稻( Oryza sativa L.) 基因组中仅有一个该基因的拷贝。Northern 分析表明水稻的ACBP基因在水稻的根、茎、叶、叶鞘、黄化苗和幼穗中皆表达,而以黄化苗的绿苗叶鞘中的表达强度高于绿苗叶片。 相似文献
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酰基辅酶A结合蛋白(acyl-CoA-binding protein,ACBP)对长链脂酰基辅酶A(long-chainfatty acyl-CoA esters,LCACoA)有很高的亲和力,因而对LCACoA在细胞内的运输和利用过程起重要的作用。本文采用RACE技术从鲈鱼肝脏中克隆了Acbp基因的全长cDNA序列,该基因全长cDNA 679 bp,5'端和3'端的非翻译区分别为83 bp和326 bp,开放阅读框为270 bp。推测编码89个氨基酸,理论等电点为5.44,分子量为10.14 kDa。鲈鱼Acbp与青鳉鱼、银鳕鱼、大西洋鲑和人的同源性分别为87%、84%、78%和68%。用RT-PCR和实时定量PCR检测鲈鱼肌肉、心脏、眼、大脑、消化道、肾脏、脂肪组织、脾脏、鳃和肝脏等10种组织的Acbp基因的表达情况,结果表明,在肾脏和肝脏的表达量高,肌肉、眼睛和大脑中表达低。定量PCR检测表明鲈鱼肝脏Acbp的表达在饥饿时明显下降,胰岛素上调其表达,葡萄糖对其表达则没有影响。 相似文献
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蛋白激酶B及其在磷脂酰肌醇3-激酶介导的信号转导中的作用 总被引:6,自引:0,他引:6
蛋白激酶B(PKB)是原癌基因c-akt的表达产物,它参与由生长因子激活的经磷脂磷肌醇3-激酶(PI3K)介导的信号转导过程。与许多蛋白激酶相似,PKB分子具有一特殊的AH/PH结构域(AH/PHdomain),后者能介导信号分子间的相互作用。PKB是PI3K直接的靶蛋白。PI3K产生的脂类第二信使PI-3,4,P2和PI-3,4,5-P3等均能与PKB和磷酸肌醇依赖性蛋白激酶(PDK)的AH/P 相似文献
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磷脂酰肌醇3-激酶在乳腺导管不典型增生癌变过程中的表达和意义 总被引:1,自引:0,他引:1
目的探讨乳腺导管不典型增生癌变过程中PI3-K p85调节亚单位的变化.方法应用免疫组织化学S-P方法检测PI3-K p85调节亚单位在乳腺正常组织及各组病变中的蛋白表达情况.结果 PI3-K p85催化亚单位在乳腺癌组表达最为明显,其阳性表达率明显高于乳腺导管单纯性增生、不典型增生组织,差异有显著性.结论 PI3-K的表达强度在一定程度上与细胞的恶性倾向有关,可能是乳腺癌发生过程中的晚期分子事件;以PI3-K信号成分作为直接切入点设计药物将为乳腺癌的治疗提供新的靶点. 相似文献
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Phosphatidylinositol 4-kinase IIIβ (PI4KB) is a host factor required for genome RNA replication of enteroviruses, small non-enveloped viruses belonging to the family Picornaviridae. Here, we demonstrated that PI4KB is also essential for genome replication of another picornavirus, Aichi virus (AiV), but is recruited to the genome replication sites by a different strategy from that utilized by enteroviruses. AiV non-structural proteins, 2B, 2BC, 2C, 3A, and 3AB, interacted with a Golgi protein, acyl-coenzyme A binding domain containing 3 (ACBD3). Furthermore, we identified previously unknown interaction between ACBD3 and PI4KB, which provides a novel manner of Golgi recruitment of PI4KB. Knockdown of ACBD3 or PI4KB suppressed AiV RNA replication. The viral proteins, ACBD3, PI4KB, and phophatidylinositol-4-phosphate (PI4P) localized to the viral RNA replication sites. AiV replication and recruitment of PI4KB to the RNA replication sites were not affected by brefeldin A, in contrast to those in enterovirus infection. These results indicate that a viral protein/ACBD3/PI4KB complex is formed to synthesize PI4P at the AiV RNA replication sites and plays an essential role in viral RNA replication. 相似文献
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Minetaro Arita 《Microbiology and immunology》2019,63(7):285-288
Phosphatidylinositol‐4 kinase III β (PI4KB) is a host factor that is required for enterovirus (EV) replication. In this study, the importance of host proteins that interact with PI4KB in EV replication was analyzed by trans complementation with PI4KB mutants in a PI4KB‐knockout cell line. Ectopically expressed PI4KB mutants, which lack binding regions for ACBD3, RAB11, and 14‐3‐3 proteins, rescued replication of poliovirus and enterovirus 71. These findings suggest that interaction of PI4KB with these host proteins is not essential for EV replication once PI4KB has been expressed and that PI4KB is functionally independent from these host proteins regarding EV replication. 相似文献
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Previously, it was found that the hepatitis C virus NS5A interacted with ACBD3 in a genotype-dependent manner. However, the region in NS5A responsible for association with ACBD3 is not clear. Domain I of NS5A was identified as critical for ACBD3 binding. By comparing the differences of amino acids in domain I from different genotypes of NS5A, it was found that key amino acids potentially corresponded to the affinity of the NS5A-ACBD3 interaction. The findings not only revealed that domain I of NS5A associates with ACBD3 but they also shed mechanistic light on how NS5A is associated with ACBD3 in a genotype-dependent manner. 相似文献
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Dominika Chalupska Bartosz Rycki Martin Klima Evzen Boura 《Protein science : a publication of the Protein Society》2019,28(12):2073-2079
Many picornaviruses hijack the Golgi resident Acyl‐coenzyme A binding domain containing 3 (ACBD3) protein in order to recruit the phosphatidylinositol 4‐kinase B (PI4KB) to viral replication organelles (ROs). PI4KB, once recruited and activated by ACBD3 protein, produces the lipid phosphatidylinositol 4‐phosphate (PI4P), which is a key step in the biogenesis of viral ROs. To do so, picornaviruses use their small nonstructural protein 3A that binds the Golgi dynamics domain of the ACBD3 protein. Here, we present the analysis of the highly flexible ACBD3 proteins and the viral 3A protein in solution using small‐angle X‐ray scattering and computer simulations. Our analysis revealed that both the ACBD3 protein and the 3A:ACBD3 protein complex have an extended and flexible conformation in solution. 相似文献
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为探讨沉默信息调节因子2(Sir2)在SCA3/MJD发病机制中的作用.选用GMR-GAL4 和Nrv2-GAL4驱动子,利用经典的GAL4-UAS系统,将含有78 个CAG 重复扩增的ataxin-3 蛋白片段(MJDtr-Q78)分别在果蝇眼睛和运动神经元内选择性表达,构建GMR-GAL4/UAS 和Nrv2-GAL4/UAS 系统SCA3/MJD 转基因果蝇模型,然后分别在抑制和不抑制自噬的情况下,使Sir2在SCA3/MJD 转基因果蝇眼睛和运动神经元内过表达.结果发现,Sir2过表达明显抑制了SCA3/MJD 转基因果蝇眼睛视网膜光感受神经元变性,显著改善了果蝇运动能力,而在自噬被抑制后,Sir2的作用效果明显减弱,表明Sir2对SCA3/MJD 转基因果蝇具有神经保护作用,而这种神经保护作用需要依赖自噬的功能. 相似文献
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Y Shinoda K Fujita S Saito H Matsui Y Kanto Y Nagaura K Fukunaga S Tamura T Kobayashi 《FEBS letters》2012,586(19):3024-3029
The metal-dependent protein phosphatase family (PPM) governs a number of signaling pathways. PPM1L, originally identified as a negative regulator of stress-activated protein kinase signaling, was recently shown to be involved in the regulation of ceramide trafficking at ER-Golgi membrane contact sites. Here, we identified acyl-CoA binding domain containing 3 (ACBD3) as an interacting partner of PPM1L. We showed that this association, which recruits PPM1L to ER-Golgi membrane contact sites, is mediated by a GOLD (Golgi dynamics) domain in ACBD3. These results suggested that ACBD3 plays a pivotal role in ceramide transport regulation at the ER-Golgi interface.
Structured summary of protein interactions
ACBD3 and PPM1Lcolocalize by fluorescence microscopy (View interaction)FYCO1physically interacts with PPM1L by pull down (View interaction)SEC14L2physically interacts with PPM1L by pull down (View interaction)ACBD3physically interacts with PPM1L by pull down (View interaction)SEC14L1physically interacts with PPM1L by pull down (View interaction)PPM1Lphysically interacts with ACBD3 by two hybrid (View interaction) 相似文献17.
Lipid phosphoinositides are master signaling molecules in eukaryotic cells and key markers of organelle identity. Because of these important roles, the kinases and phosphatases that generate phosphoinositides must be tightly regulated. Viruses can manipulate this regulation, with the Type III phosphatidylinositol 4-kinases (PI4KA and PI4KB) being hijacked by many RNA viruses to mediate their intracellular replication through the formation of phosphatidylinositol 4-phosphate (PI4P)-enriched replication organelles (ROs). Different viruses have evolved unique approaches toward activating PI4K enzymes to form ROs, through both direct binding of PI4Ks and modulation of PI4K accessory proteins. This review will focus on PI4KA and PI4KB and discuss their roles in signaling, functions in membrane trafficking and manipulation by viruses. Our focus will be the molecular basis for how PI4KA and PI4KB are activated by both protein-binding partners and post-translational modifications, with an emphasis on understanding the different molecular mechanisms viruses have evolved to usurp PI4Ks. We will also discuss the chemical tools available to study the role of PI4Ks in viral infection. 相似文献
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Hsp22对SCA3/MJD转基因果蝇的神经保护作用研究 总被引:1,自引:0,他引:1
为了探讨Hsp22在SCA3/MJD发病机制中的作用.选用GMR-GAL4和elav-GAL4驱动子,利用经典的GAL4-UAS系统,将含有78个CAG重复扩增的ataxin-3蛋白片段(MJDtr-Q78)分别在果蝇眼睛和神经系统选择性表达,构建GMR-GAL4/UAS和elav-GAL4/UAS系统SCA3/MJD转基因果蝇模型, 然后利用遗传学方法和热休克反应使Hsp22在SCA3/ MJD转基因果蝇眼睛和神经系统以不同水平过表达.结果表明,Hsp22过表达显著抑制了MJDtr-Q78蛋白的神经毒性,果蝇眼睛视网膜光感受神经元变性明显缓解,果蝇存活能力也显著提高.Hsp22对SCA3/MJD具有保护作用,增强Hsp22表达对SCA3/MJD可能是一种潜在的治疗方法. 相似文献
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Zhang Qiuhan Li Siliang Lei Ping Li Zixian Chen Feifei Chen Qi Wang Yulu Gong Jiami Tang Qi Liu Xinjin Lan Ke Wu Shuwen 《中国病毒学》2021,36(6):1387-1399
Virologica Sinica - Similar to that of other enteroviruses, the replication of enterovirus 71 (EV71) occurs on rearranged membranous structures called replication organelles (ROs).... 相似文献
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Jonathan H. Epstein Jeffrey McKee Phil Shaw Vicki Hicks Gino Micalizzi Peter Daszak A. Marm Kilpatrick Gretchen Kaufman 《EcoHealth》2006,3(4):290-298
Over the last 20 years, Australian white ibis populations (Threskiornis molucca) have expanded into urban areas, leading to increased contact between ibis, domestic animals, and humans. This has led to
concern that ibis may transmit pathogens that threaten public health or food production. Here we report results from a study
of ibis viral serology and bacterial culture that indicate that ibis are hosts of zoonotic and livestock pathogens such as
Salmonella spp., Newcastle disease virus, avian influenza virus, and flaviviruses in Australia. We also performed a behavioral study
to measure contact rates among ibis, people, and livestock that determine the potential for disease transmission. 相似文献