精浆α-糖苷酶和酸性磷酸酶活性与顶体酶活性的关系

目的研究精子顶体酶活性与精浆α-糖苷酶和酸性磷酸酶活性的关系。方法分光光度比色法测定精子顶体酶、酸性磷酸酶和α-1，4糖苷酶活性一结果精浆α-糖苷酶和酸性磷酸酶活性异常组顶体酶活性均明显低于其相应正常组(P＜0．001，P＜0．001)。顶体酶活性与精浆α-糖苷酶活性、酸性磷酸酶活性之间存在明显正相关(P＜0．001，P＜0．001)。结论精浆α-糖苷酶和酸性磷酸酶活性影响精子顶体酶活性。     

不育病人精浆前列腺特异性抗原的检测及意义

目的 :探讨不育病人精浆前列腺特异抗原 (PSA)检测的意义。　方法 :随机选择 85例不育病人 ,采用ELISA法检测不育病人精浆PSA水平 ,并分析其与精浆酸性磷酸酶 (ACP)、精子密度、活率之间的关系。　结果 :35例不液化病人、30例液化不全病人精浆PSA水平、ACP浓度、精子活率 (a +b +c级精子百分率 )均显著低于 2 0例液化正常病人 (P <0 .0 1) ;而精子密度则无差异 (P >0 .0 5 )。精浆PSA水平与精浆ACP浓度、精子活率显著正相关 (P<0 .0 1)。　结论 :精浆PSA水平与精液液化密切相关 ,其质和量的异常会使精子活率降低 ,从而导致男性生育力下降     

精浆弹性蛋白酶水平与精液主要参数和精子功能的相关性分析

目的:探讨精浆弹性蛋白酶与精液主要参数和指标的关系。方法:用酶联免疫吸附法(ELISA)检测精浆中的弹性蛋白酶,按照WHO人类精液实验室手册要求进行精液常规分析、精子形态分析,检测精子顶体酶活性、精浆抗体(AsAb)、解脲支原体等,分析弹性蛋白酶与男性不育相关因素的关系。结果:209例男性不育患者中,43例患者精浆弹性蛋白酶≥290ng/ml,设为炎症组;166例患者精浆弹性蛋白酶<290ng/ml,设为非炎症组。炎症组的精子密度、精子活动率、a+b级活力精子率、精子顶体酶阳性率均低于非炎症组(P<0.05);而精子畸形率、精浆抗体(AsAb)、解脲支原体阳性率均高于非炎症组(P<0.05)。两组的精液量、PH值和液化时间差异无统计学意义。结论:精浆弹性蛋白酶水平与精液质量有密切的关系,生殖道感染是导致男性不育的重要原因。     

不育男性精子密度、活动率、活力与顶体酶活性关系的研究

目的探讨不育男性精子密度、活动率、活力、与精子顶体酶活性的关系.方法采用分光光度比色法测定精子顶体酶活性,并将其与精子密度、活动率和活力进行统计分析.结果随着精子密度、活动率、活力降低,精子顶体酶活性均明显降低(各组间比较均P＜0.001).结论不育男性精子顶体酶活性降低可影响精子密度、活动率和a, b级活力精子率,精子顶体酶活性可作为男性不育精子质量检测的指标之一.     

人精子顶体酶活性及其影响因素分析

为研究人精子顶体酶活性与男性不育的关系，分别测定门诊３５６例不育男性精液（实验组）和１８例正常生育者精液（对照组）的顶体酶活性，同时测定实验组男性的精子活力、精子活率、畸形率、镜下白细胞数、精浆抗精子抗体并进行解脲支原体培养，作相关性分析。结果：实验组和对照组顶体酶活性为１０７±９２和２９７±１４３μＩＵ／１０６精子，组间比较差异有显著性（Ｐ＜０００１），顶体酶活性与镜下白细胞数、畸形率明显负相关，与精子活率、精子活力正相关，顶体酶活性与解脲支原体感染有关，但精浆抗精子抗体的存在不影响顶体酶活性。提示顶体酶活性与精子质量有关，是影响生育的重要因素     

精浆免疫抑制物质与抗精子抗体等参数的关系

本文报告了精浆免疫抑制物质(SPIM)活性与精子活率、密度、精子顶体酶活力、精浆果糖和血清抗精子抗体阳性率的比较研究。结果表明SPIM活性与精子活率、密度呈正相关;不育组、流产组的SPIM活性与ASA阳性率和精子顶体酶活率无显著差异(P>0.05);而SPIM活性、精子顶体酶活力均较生育组低,ASA阳性率均较生育组高(P<0.05～P<0.01),生育、不育和流产组之间果糖浓度均有显著差异(P<0.05～P<0.01)。     

男性不育患者精液质量与精子顶体酶活性关系分析

目的:分析男性不育患者精液参数与精子顶体酶活性变化,探讨精液质量与顶体酶活性的关系。方法:对214例男性不育患者的精液作精子顶体酶活性、弹性蛋白酶、果糖、α葡糖苷酶、锌、酸性磷酸酶、精子尾部低渗肿胀试验检测,同时精子质量检测仪作精液分析。以检出精子顶体酶活性正常的(48.2~218.7μIU/106精子)111例作为对照组,与顶体酶活性异常(<48.2μIU/106精子)的103例精液参数作对比分析。结果:两组精液参数的精子密度、精子活动率、a+b级精子百分率、精子尾部低渗肿胀试验差异均有非常显著性(P<0.001)。弹性蛋白酶差异有显著性(P<0.05)。果糖、α葡糖苷酶差异亦有显著性(P<0.05)。精液量、锌、酸性磷酸酶差异无显著性(P>0.05)。结论:精子顶体酶活性与精液质量关系密切,精子顶体酶活性是反映精液质量的一项可靠指标。     

1077例男性不育患者精子顶体酶活性及其影响因素分析

本研究对１０７７ 例不育门诊病人的精子进行顶体酶活性检测,并分析了某些因素对顶体酶活性的影响。研究发现,精子顶体酶活性呈正偏态分布,６５ ．５ ％ 低于正常值（１８ μ Ｉ Ｕ／１０６ 精子） ;相关检验表明,顶体酶活性与精子活率、活力呈显著正相关,与精子畸形率和镜下精液白细胞呈显著负相关;卡方检验表明,顶体酶活性与支原体感染、精浆抗精子抗体无关;秩和检验表明,顶体酶活性降低组精液粘度高于顶体酶活性正常组（ Ｐ＜ ０ ．０００５） 。     

人精浆中性α-糖苷酶活性与精子运动相关性研究

本文用改良精浆中性α-糖苷酶活性测定法和精液自动分析仪测定了40例正常有生育力者和22例经输精管向附睾方向注射0.3～0.5ml/侧鱼肝油酸钠(SM)节育术者的精浆中性α-糖苷酶活性及精子运动的有关指标。结果表明:1.正常人精浆中性α-糖苷酶活性为23.31±15.31mU/ml;SM节育组为4.46±3.79mU/ml,两组之间有高度显著性差别(P相似文献   

人精浆抗精子抗体与顶体酶活性关系的探讨

目的 :研究精浆抗精子抗体 (AsAb)对精子顶体酶活力的影响。　方法 :用间接血凝法测定 34 32例不育男性和 6 5例生育男性精浆的AsAb ,并对其中的 2 882例不育男性精子用比色法进行了顶体酶活性的测定。　结果 :34 32例不育者精浆AsAb阳性率为 10 .2 0 %,2 882例进行了顶体酶活性测定的不育者精浆AsAb阳性率为 9.37%,对照组精子顶体酶活性明显高于各不育组 (P <0 .0 0 1)。不育组AsAb阳性组与AsAb阴性组比较顶体酶活性没有明显差异 (P >0 .0 5 ) ,顶体酶活性正常与异常组AsAb阳性率比较差异无显著性 (P >0 .0 5 )。　结论 :精浆AsAb对精子顶体酶活性没有影响。     

精子DNA损伤、核蛋白组型转换与顶体酶活性及精液参数的相关性分析

目的:探讨精子DNA损伤、精子核蛋白组型转换与顶体酶活性及精液参数的相关性。方法:收集535例精液标本,采用精子染色质扩散(sperm chromatin dispersion,SCD)检测精子DNA损伤,并与精子核蛋白组型转换、顶体酶活性和WHO手册(第4版)精液参数进行相关性分析。结果:精子DNA损伤与精子核蛋白组型转换、顶体酶活性、精子浓度及前向运动精子这些指标之间比较均具有显著性差异(P<0.01);精子DNA损伤与年龄、核蛋白组型转换、精子浓度和D级精子比例之间呈显著正相关(P<0.01或P<0.05),而与顶体酶活性呈显著负相关(P<0.001),多元逐步回归分析显示年龄、精子浓度、D级精子比例、核蛋白组型转换及顶体酶活性是5个独立的相关变量。精子核蛋白组型转换、顶体酶活性、精子密度和前向运动精子这4个指标的异常率在精子DNA异常组(DFI≥30%)中均显著的高于正常组(DFI<30%)。结论:精子DNA损伤与精子核蛋白组型转换、顶体酶活性及WHO(第4版)精液各参数之间存在密切的联系,可能是评价精子质量的另一项重要的指标。     

Studies on relationship between semen viscosity and other semen parameters, Ureaplasma urealyticum infection and seminal plasma antisperm antibody

Objective: To study the relationship between semen viscosity and other semen parameters, Ureaplasma urealyticum (UU) infection and seminal plasma antisperm antibody (AsAb) in male infertiles. Methods: Semen parameters, Ureaplasma urealyticum (UU) infection and antisperm antibody (AsAb) were measured and analyzed in 4337 infertile men. Results: The seminal viscosity was higherr than normal in 65.02 % of 4337 male infertiles. The sperm motility and grade (a, b) motile sperm were significantly lower in the high viscosity group than in the normal viscosity group (P<0.05-0.01). The rate of abnormal morphology sperm was higher and duration of semen liquefaction was longer in the high viscosity than in the normal viscosity group (P<0.01). The seminal volume, sperm concentration and semen pH were not significantly different between the two groups. The semen viscosity is significantly higher in subjects with higher seminal WBC (>5/ HP) than in those with lower WBC (<5/HP). The positive AsAb and UU infection rates     

Relationships between seminal plasma arginase activity and spermatological parameters in rams

This study was conducted to evaluate the correlation between seminal plasma arginase activity and spermatological parameters in rams. In this study, five fertility-proven Awassi rams were used as material. Six ejaculates were collected from each ram by an artificial vagina. Spermatological parameters (semen volume, mass activity, sperm motility and concentration and abnormal sperm rate) were immediately determined in each ejaculate. For enzyme assay, the semen samples were centrifuged and stored at -20 °C for the analysis of arginase activity. The average seminal plasma arginase activity was 0.61 ± 0.20 U (mg protein)(-1) . There was a positive correlation between arginase activity and semen volume (r = 0.412, P < 0.05), semen mass activity (r = 0.610, P < 0.01), sperm motility (r = 0.447, P < 0.05) and sperm concentration (r = 0.808, P < 0.01). However, there was a negative correlation between arginase activity and abnormal sperm rate (r = -0.424, P < 0.05). In conclusion, this study clearly suggests that there is a significant correlation between seminal plasma arginase activity and spermatological parameters. In light of these results, seminal plasma arginase activity may be a biochemical criterion for determining sperm quality besides classical semen analysis parameters in rams.     

男性年龄与精子顶体酶活性及精子DNA碎片指数的相关性分析

目的探讨男性年龄与精子顶体酶活性、精子DNA碎片指数(DFI)的相关性。方法选取2016年1~8月在我院生殖医学中心就诊的436例不育症男性患者为研究对象,所有患者均行精液常规检查、精子顶体酶活性检查和(或)精子DFI分析。将患者按年龄分为<30岁、30~39岁、≥40岁3组,分析各组的精液常规、顶体酶活性及精子核DFI的差异及其相关性。结果不同年龄段患者的体重指数(BMI)、禁欲天数、精液量无显著性差异(P>0.05);年龄≥40岁组患者的前向运动精子百分率、活动精子百分率及精子顶体酶活性显著低于<30岁和30~39岁组(P<0.05);≥40岁组患者的精子DFI显著高于<30岁和30~39岁组(P<0.05)。年龄与前向运动精子百分率及活动精子百分率之间呈负相关(P<0.05),但是相关性较弱。精子顶体酶活性与精子正常形态率、前向运动精子百分率、非前向运动精子百分率、活动精子百分率呈正相关(P<0.05);精子DFI与年龄、禁欲天数、前向运动精子百分率呈正相关(P<0.01),与精液量、精子浓度、活动精子百分率呈负相关(P<0.05);精子顶体酶活性和DFI之间无相关性(P>0.05)。结论年龄增长会导致精液前向运动精子百分率、活动精子百分率、精子顶体酶活性、DFI等参数改变,直接或间接影响男性生育力。说明年龄对男性不育的影响是多方面的,建议有生育需求的大龄(≥40岁)男性尽早进行生育咨询与评估。     

Effect of smoking on seminal plasma ascorbic acid in infertile and fertile males

This work aimed to assess the relationship of seminal ascorbic acid levels with smoking in infertile males. One hundred and seventy men were divided into four groups: nonobstructive azoospermia [NOA: smokers (n = 20), nonsmokers (n = 20)]; oligoasthenozoospermia [smokers (n = 30), nonsmokers (n = 20)]; asthenozoospermia [smokers (n = 20), nonsmokers (n = 20)] and normozoospermic fertile men [smokers (n = 20), nonsmokers (n = 20)]. The patients underwent medical history, clinical examination, conventional semen analysis and estimation of ascorbic acid in the seminal plasma calorimetrically. There was a significant decrease in the mean seminal plasma ascorbic acid levels in smokers versus nonsmokers in all groups (mean +/- SD; 6.03 +/- 2.18 versus 6.62 +/- 1.29, 7.81 +/- 1.98 versus 9.44 +/- 2.15, 8.09 +/- 1.98 versus 9.95 +/- 2.03, 11.32 +/- 2.15 versus 12.98 +/- 12.19 mg dl(-1) respectively). Fertile subjects, smokers or not, demonstrated significant higher seminal ascorbic acid levels than any infertile group. Seminal plasma ascorbic acid in smokers and nonsmokers was correlated significantly with sperm concentration (r = 0.59, 0.60, P < 0.001), sperm motility (r = 0.65, 0.55, P < 0.001) and negatively with sperm abnormal forms per cent (r = -0.53, -0.50, P < 0.001). Nonsignificant correlations were elicited with semen volume (r = 0.2, 0.09) or liquefaction time (r = 0.03, 0.06). It is concluded that seminal plasma ascorbic acid decreased significantly in smokers and infertile men versus nonsmokers and fertile men, and is significantly correlated with the main sperm parameters: count, motility and normal morphology. Also, cigarette smoking is associated with reduced semen main parameters that could worsen the male fertilizing potential, especially in borderline cases.     

Problems in evaluating male fertility: valuable factors in evaluating male fertility and normal values of seminal parameters

To determine the valuable factor for evaluating male fertility, a comparative study was done as to various seminal parameters between fertile and infertile groups. The fertile group consists of 57 proven fertile males and the infertile group consists of randomly chosen 67 infertile patients. Seminal parameters assessed were sperm concentration, motility, mean velocity, total sperm output, total motile sperm output, sperm morphology, acrosin activity and sperm penetration rate on zona-free hamster egg penetration assay (SPA). The infertile group was significantly different from the fertile group in every parameter except acrosin activity. However, the range of each parameter in the two groups overlapped each other. The diagnostic rate of each parameter, which is the percentage of an infertile male correctly diagnosed as infertile, was calculated by using 95% specificity threshold value of fertile males. The 95% specificity threshold values of sperm concentration, motility and % normal shaped sperm were 24.9 x 10(6)/ml, 34.9% and 55%, respectively, and they could be acceptable for the normal limit of seminal parameters. The diagnostic rate was highest in penetration rate (72.4%). In other words, penetration rate is the most valuable factor in various parameters for making a distinction between fertile and infertile males. Sperm motility and mean velocity showed the next highest diagnostic rate. On the other hand, sperm concentration showed a poor diagnostic rate (36.8%). In addition, there was no significant correlation between penetration rate and any other seminal parameters. These results suggest that the SPA will be an essential test for evaluating male fertility and penetration rate may be a marker of male fertility in the treatment of male infertility.