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??Abstracts?? Objective To investigate the expression of RLX in blood and renal tissue of patients with HSP?? and its correlation with clinical indexes and pathological changes?? and explore its effect on the pathogenesis of HSP. Methods Collect 39 cases of Henoch-Schonlein purpura ??HSP?? from Department of Nephrology in Children’s Hospital of Jiangxi province from November 2011 to June 2012?? with complete follow-up data. According to the clinical urine manifestation?? 39 patients with HSP were divided into non-kidney damage group ??n=20?? and kidney damage group ??n=19??. All 39 patients were collected blood samples in the acute and convalescent phase. Nineteen patients from kidney damage group were divided into mild lesions ??n=5???? moderate lesions ??n=9??and severe lesions ??n=5???? according to the degree of pathological changes. RLX concentration was detected with enzyme-labeled immunosorbent assay ??ELISA?? method.RLX protein expression in renal tissue was detected with immunohistochemistry method. Results In the acute phase?? the serum level of RLX in HSP was significantly higher than normal control?? and its concentration was significantly higher in kidney damage group than in non-kidney damage group ??P??0.05??. Compared with acute phase?? the serum level of RLX in HSP in the convalescent phase was significantly lower?? and the level of decline was slower in kidney damage group than in non-kidney damage ??P??0.05??. Weak expression of RLX in renal tissue was found in normal control??while the expression of RLX in renal tissue significantly increased in HSPN ??P??0.05???? with the pathological changes of renal tissue aggravating. The expression of RLX in renal tissue in HSPN was significantly correlated with the serum concentration of RLX ??P??0.05?? and the total urine protein for 24 hours ??P??0.05??. Conclusion There is a modest up-regulation in serum and renal RLX protein expression in HSP?? and the severity of the disease is closely related with RLX expression?? which suggests that RLX may be involved in the pathogenesis of HSP/HSPN?? and it may be an important mechanism that upregulation of RLX may play a defensive role in delaying disease progression of HSP/HSPN.     

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目的研究婴幼儿肺炎并发不同程度心力衰竭时内皮素(ET)、D-二聚体(D-dimer)、纤维蛋白原(FBG)等变化及其内在联系,探讨该阶段血管内皮功能、凝血、纤溶功能的变化。方法选择2005-12—2006-8于邯郸市中心医院儿科就诊的肺炎及肺炎并发不同程度心力衰竭患儿80例作为观察组,健康婴幼儿20例作为对照组,均抽血检测ET、D-dimer、FBG、活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)、凝血酶时间(TT)值。结果各组间ET、D-dimer、FBG、APTT存在统计学差异,心衰程度越重,ET、D-dimer、FBG越高,APTT越低,其中以肺炎并发重度心衰组明显,ET与D-dimer间呈正相关(r=0.42,P<0.01);D-dimer与FBG间也呈正相关(r=0.30,P<0.01)。结论婴幼儿肺炎并发心力衰竭时存在内皮细胞功能紊乱和凝血、纤溶系统的激活。     

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目的研究肺炎支原体肺炎(MPP)患儿血清及诱导痰中白细胞介素-4(IL-4)及γ-干扰素(IFN-γ)质量浓度变化,探讨其在MPP发病机制中的作用。方法2003年2~8月对中国医科大学附属二院儿科住院的MPP患儿38例,用ELISA法测定38例血清及24例诱导痰中急性期及恢复期的IL-4及IFN-γ质量浓度。结果MPP患儿血清及诱导痰IL-4质量浓度急性期明显高于恢复期和正常对照组(P<0·01);IL-4/IFN-γ比值急性期高于恢复期和正常对照组(P<0·05)。MPP患儿急性期血清及诱导痰IL-4质量浓度重症组明显高于轻症组(P<0·01),急性期血清及诱导痰IL-4/IFN-γ比值重症组高于轻症组(P<0·05);诱导痰与血清两种标本检测结果一致,诱导痰中的质量浓度高于血清中的质量浓度,两者呈正相关。结论MPP患儿存在TH1/TH2功能紊乱,MPP患儿TH2反应占优势,IL-4及IFN-γ参与MPP患儿的免疫状态改变,在MPP发病机制中发挥一定作用。     

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目的研究肺炎支原体肺炎(MPP)患儿血清及诱导痰中白细胞介素2(IL-2)和白细胞介素6(IL-6)的表达,探讨其在MPP感染发病机制中的作用及临床意义。方法收集华中科技大学同济医学院附属孝感医院儿科2009年6月至2010年12月住院确诊的56例MPP患儿,采用酶联免疫吸附实验(ELISA法)检测56例患儿血清及42例患儿诱导痰中IL-2和IL-6的质量浓度,用t检验比较患儿血清及诱导痰中IL-2、IL-6在不同病期和轻重症中的表达水平。以同期40名体检的儿童作为正常对照组。结果 MPP患儿血清及诱导痰中IL-2在急性期明显低于恢复期和正常对照组(P<0.05),IL-6在急性期明显高于恢复期和正常对照组(P<0.05);MPP患儿血清及诱导痰中IL-2在急性期和恢复期中轻症组均高于重症组(P<0.05),IL-6在急性期和恢复期中轻症组均低于重症组(P<0.05);血清和诱导痰中两种标本检测结果一致,且诱导痰中IL-2和IL-6总体上高于血清组,两者呈正相关。结论 IL-2和IL-6在MP感染发病机制中起重要作用。MP感染初期可通过检测血及诱导痰中的IL-2和IL-6,为病情严重程度及预后作出深入评估和判...