星形细胞瘤中PTEN和CB表达与侵袭性的关系

目的 探讨ＰＴＥＮ和组织蛋白酶－Ｂ（ＣＢ）表达与星形细胞瘤恶笥程度和侵袭性的关系。方法 应用免疫组化方法检测５６例星形细胞瘤标本中ＰＴＥＮ和ＣＢ的表达,分析其与肿瘤恶笥程度和侵袭性的关系。结果 ＰＴＥＮ阳性染色主要定位于细胞质中,５６例星形细胞瘤中ＰＴＥＮ阳性表达３４例（６１％）,高分化组（Ⅰ和Ⅱ组）２３／２７例（８５％）和低分化组（Ⅲ和Ⅳ级）１１／２９例（３８％）之间差异显,明显高于低分化组（     

人脑胶质瘤金属硫蛋白表达的生物学意义

目的 研究金属硫蛋白（ＭＴ）在人脑各级胶质瘤中表达的生物学意义,方法 利用免疫组织化学方法检测４８例胶质瘤手术标本中金属硫蛋白及增殖细胞核抗原（ＰＣＮＡ）的表达水平,比较不同级别胶质瘤中ＭＴ的表达与胶质瘤级别的关系及其与ＰＣＮＡ相关性,结果 随着胶质瘤级别的增高,ＭＴ和ＰＣＮＡ的表达率逐渐升高,ＭＴ在脑胶质瘤中的表达率分别为Ⅰ级３８．０％,Ⅱ级４６．７％,Ⅲ级６６．７％,Ⅳ级７５．０％,低恶性度组     

FHIT、PTEN在人脑胶质瘤中的表达和意义

目的 检测人脑胶质细胞瘤中脆性组氨酸三联体基因（FHIT）、第10号染色体同源丢失性磷酸酶-张力蛋白基因（PTEN）的表达情况.方法 采用S-P免疫组织化学法.结果 FHIT和PTEN阳性染色定位于细胞浆,52例胶质瘤标本中,有39例FHIT呈阳性表达,低度恶性肿瘤（Ⅰ～Ⅱ级）的阳性表达率明显高于高度恶性（Ⅲ～Ⅳ级）肿瘤（P 〈0.05）;而有37例PTEN呈阳性表达,低度恶性肿瘤的阳性表达率明显高于高度恶性肿瘤（P〈0.05）.FHIT与PTEN在脑胶质瘤细胞中的表达呈正相关.结论 FHIT与PTEN的表达与脑胶质瘤的发生、发展密切相关,联合检测FHIT、PTEN在胶质瘤中的表达有助于对肿瘤细胞增殖能力、分化程度做出正确评价.     

P53蛋白在人脑胶质瘤中的表达及与增殖的关系(附39例检测结果分析)

目的：观察Ｐ５３蛋白及增殖细胞核抗原（ＰＣＮＡ）在人脑胶质瘤中的表达情况，探讨Ｐ５３蛋白表达与人脑胶质瘤的增殖活性和临床病理类型之间的关系。方法：采用免疫组化ＳＰ法对３９例人脑胶质瘤石蜡标本中Ｐ５３蛋白及ＰＣＮＡ进行检测。结果：在３９例胶质瘤中１３例观察到了Ｐ５３蛋白表达。Ｐ５３蛋白阳性表达在星形细胞瘤、间变性星形细胞瘤和胶质母细胞瘤中分别为１８７％、４５４％和７１％。Ｐ５３蛋白表达与增殖细胞核抗原阳性率密切相关。结论：Ｐ５３蛋白的表达与胶质瘤的病理类型、恶性程度和增殖活性相关。在常规石蜡包埋标本中，使用免疫组化法检测Ｐ５３蛋白的表达情况，对肿瘤的预后评估具有积极的参考价值     

磷酸甘油酸变位酶1在脑胶质瘤中的表达及意义

目的：研究磷酸甘油酸变位酶1（PGAM1）在脑胶质瘤细胞中的表达情况,探讨PGAM1在胶质瘤恶性生长中的作用。方法：应用逆转录聚合酶链反应检测PGAM1在大鼠C6胶质瘤细胞和星形胶质细胞中的表达。应用免疫组织化学法检测人脑胶质瘤组织和瘤周脑组织中PGAM1蛋白的表达。结果：（1）PGAM1在C6胶质瘤细胞中表达显著高于星形胶质细胞（P〈0.05）。（2）对照组15例瘤周脑组织中PGAM1阴性12例,阳性表达3例（20.00%）。观察组43例脑胶质瘤标本中PGAM1阳性表达29例,PGAM1阴性14例（67.44%）。PGAM1两组表达率比较差异有统计学意义（χ2=8.29,P〈0.05）。结论：PGAM1可能与人脑胶质瘤的恶性生长有关。     

E选择素、P选择素在人脑胶质瘤中的表达意义

目的通过检测E选择素、P选择素在人脑胶瘤中的表达,探讨其与胶质瘤浸润发展的关系。方法采用免疫组织化学SP法分别检测E选择素、P选择素在61例人脑胶质瘤和8例正常人脑组织中的表达。结果①E选择素、P选择素在胶质瘤组中的阳性表达率分别为60.7%、57.4%;较对照组明显增高（P〈0.05）。②E选择素在胶质瘤Ⅰ～Ⅱ级组和Ⅲ～Ⅳ级组中的阳性表达率分别为46.9%、75.9%,随肿瘤级别增高表达水平也明显增高（P〈0.05）。P选择素在不同级别胶质瘤中的表达率差异无统计学意义（P〉0.05）。③E选择素、P选择素在人脑胶质瘤中的表达无显著相关（R=0.14498,P=0.2649）。结论E选择素、P选择素可能与人脑胶质瘤的浸润发展有关。     

胃癌组织中神经内分泌细胞检测及临床研究

应用微波辐射ＳＰ方法检测１００例胃癌中嗜铬素Ａ（ＣｇＡ）、突触素（ＳＹＮ）表达。结果３４例胃癌含神经内分泌细胞（ＮＥ）、阳性率３４％，４例符合胃内分泌细胞癌，３０例符合胃癌伴神经内分泌分化。ＮＥ阳性癌细胞多出现分化差的胃癌组织类型中与进展期胃癌中。ＮＥ阳性３４例胃癌淋巴结转移２７例（７９．４％），较高于ＮＥ阴性６６例胃癌淋巴转移４２例（６３．６％），３年生存率５８．３％较低于ＮＥ阴性胃癌７０．８％     

生存素在细胞核及细胞质中的表达与脑胶质瘤患者预后的关系

目的分析生存素（survivin）在细胞核、细胞质中的表达与脑胶质瘤患者各临床病理学参数及预后的关系。方法应用组织芯片技术结合免疫组织化学方法检测94例胶质瘤患者中survivin蛋白质细胞核、细胞质的表达情况,并结合临床随访资料进行分析。结果94例胶质瘤患者中有21例（22．3％）为survivin细胞核阳性表达,82例（87．2％）为survivin细胞质阳性表达。病理Ⅰ～Ⅱ级与Ⅲ～Ⅳ级的survivin细胞核阳性表达率分别为7．3％、34．0％（P〈0．01）;复发与无复发胶质瘤的survivin细胞核阳性表达率分别为29．8％、10．8％（P〈0．05）。survivin细胞核表达阳性组与阴性组的5年总生存率分别为0％、39．50％（P〈0．01）,3年无进展生存率分别为0％、27．52％（P〈0．05）;survivin浆表达阳性组与阴性组的5年总生存率分别为43．60％、13．85％（P〉0．05）,3年无进展生存率分别为23．88％、0％（P〉0．05）。多因素分析显示：survivin细胞核表达是总生存率的独立预后因子。结论survivin细胞核表达与胶质瘤分化程度呈负相关,是胶质瘤患者总生存率和无进展生存率预后的不利因素;而survivin细胞质表达与胶质瘤的预后无关。     

基质金属蛋白酶-2的表达与胶质瘤恶性度相关性研究

目的探讨基质金属蛋白酶-2（MMP-2）的表达与人脑胶质瘤恶性程度及预后的关系。方法采用免疫组织化学SP法检测45例不同病理分级的人脑胶质瘤及10例正常脑组织标本中MMP-2的表达,测定其阳性细胞数。结果胶质瘤中MMP-2表达于肿瘤细胞胞浆和血管内皮细胞;不同病理分级胶质瘤中MMP-2阳性表达率分别为Ⅰ级及Ⅱ级65.0%,Ⅲ级86.7%,Ⅳ级100.0%,显著高于正常脑组织（P〈0.01）,且与胶质瘤恶性程度呈正相关（r=0.627,P〈0.05）。结论MMP-2的表达与胶质瘤恶性程度密切相关,可作为临床判断胶质瘤恶性程度、侵袭性及预后的重要指标。     

EGF、EGFR及PCNA在肺癌中的表达

用Ｓ－Ｐ免疫组化方法对５２例原发性肺癌作ＥＧＦ、ＥＧＦＲ和ＰＣＮＡ检测。结果显示,４６例ＮＳＣＬＣ中ＥＧＦ、ＥＧＦＲ的阳性表达率分别为６０．９％和６３％,６例小细胞癌和正常肺组织无表达。有转移的肺癌组ＥＧＦＲ的阳性表达率高于无转移组（Ｐ〈０．０５）。５２例肺癌中ＰＣＮＡ的阳性表达率为７８．８％。ＮＳＣＬＣ中ＥＧＦ、ＥＧＦＲ的表达与肺癌分期、病理分级以及ＰＣＮＡ的表达呈正相关。以上结果提示ＥＧＦ、Ｅ     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.