红金消结片联合康妇消炎栓治疗卵巢囊肿的临床疗效

目的：探讨红金消结片联合康妇消炎栓治疗卵巢囊肿临床疗效。方法选取2013年3月～2015年1月我院收治96例卵巢囊肿患者随机分为研究组和对照组,对照组给予红金消结片治疗,研究组给予红金消结片联合康妇消炎栓治疗,比较两组患者治疗效果。结果研究组治疗总有效率为97.92％高于对照组85.42％,比较差异有统计学意义（P＜0.05）。结论红金消结片联合康妇消炎栓治疗卵巢囊肿较单纯红金消结片治疗效果更为显著。     

红金消结片联合康妇消炎栓治疗卵巢囊肿的临床研究

目的：探讨口服红金消结片联合康妇消炎栓直肠给药治疗卵巢囊肿的临床疗效。方法卵巢囊肿患者中100例,随机分为治疗组和对照组,每组50例。治疗组给予红金消结片口服联合康妇消炎栓直肠给药治疗,对照组组给予红金消结片口服。观察2组患者的治疗效果。结果治疗组患者治愈率为78．0％,总有效率为98．0％;对照组患者治愈率为54．0％,总有效率68．0％,差异均有统计学意义（ P ＜0．05）。结论口服红金消结片联合康妇消炎栓直肠给药治疗卵巢囊肿比单纯口服红金消结片治疗更有效,可供临床参考并应用。     

碘伏消毒液毒理学试验研究

目的研究碘伏（AEO-1）消毒液使用的安全性。方法采用小鼠急性经口毒性、小鼠骨髓细胞微核、一次破损皮肤刺激、多次完整皮肤刺激、急性眼刺激、一次阴道黏膜刺激及亚急性毒性等试验。结果该碘伏消毒液LD50大于5000mg／kg,属实际无毒,小鼠骨髓细胞微核试验为阴性,碘伏消毒液原液对家免皮肤、眼及阴道黏膜刺激的刺激指数均为0,均属无刺激性;亚急性毒性试验,当高剂量组为1．0g／kg时,各项检测指标均未观察到异常变化。结论提示AEO-1为载体的碘伏消毒液在使用浓度下是安全的。     

消炎散质量标准研究

目的 :建立消炎散的质量标准。方法 :采用薄层色谱法对方中大黄、金银花、栀子进行定性鉴别 ;采用高效液相色谱法测定大黄素、大黄酚的含量。结果 :大黄素的线性范围为21 12～105 6ng/ml(r=0.9998 ,n=5) ,平均回收率为98.78 % ;大黄酚的线性范围为29 28～146 4ng/ml(r=0 9998 ,n=5) ,平均回收率为99.45 %。结论 :本方法操作简便、可靠 ,重现性好 ,专属性强 ,可作为消炎散的内在质量控制方法     

栀消炎生肌水提取工艺的研究

消炎生肌水是以黄连、栀子等药味经醇提水沉后制成的外用水剂,临床应用30余年,具有良好的清热解毒、活血化瘀、消炎止痛、祛腐生肌之功效。本文采用正交试验法以处方中主药黄连所含成分生物碱的提取量及该方体外抑菌试验为指标,综合评定,对制备过程中影响回流提取效果的诸因素进行探讨,选择最佳提取条件,为消炎生肌外用水剂提取工艺的优选提供实验依据。1仪器与材料实验所用药材均由珠海市药材公司购得。盐酸小檗碱对照品由中国约品生物制品检定所提供;中性氧化铝由上海五四化学试剂厂生产;金黄色葡萄球菌（ATCC260003）、乙型溶…     

癌痛膏(散)镇痛、消炎作用的临床及实验研究

目的 开发一个低毒无成瘾性但有镇痛抗癌作用的中药制剂。方法及结果：临床观察 对３６例晚期癌痛患者外敷癌痛膏镇痛有效率９４％，显效率５５．４％，用药半年，无成瘾，无肝肾损伤，动物实验，镇痛；膏剂外敷，对抗甲醛引起的疼痛（１０ｍｉｎ）起效，持续１ｈ以上，消肿，对巴豆油致小鼠耳肿胀抑制率３５．５％，虽低于考的松５０．６％，证明对无菌性炎症消肿功效可靠，抑菌试验上显示该药对金葡菌，大肠杆菌，克雷白肺炎杆菌     

止血消炎散质量标准的研究

目的采用HPLC法和络合滴定法测定止血消炎散中大黄素与氧化锌的含量.方法络合滴定法采用自动滴定装置,滴定液为0.05mol/L乙二胺四乙酸二钠(F=1.0480).色谱柱Techsphere ODS 5μ(250mm×4.6mm),带保护柱(12mm×4.6mm);流动相甲醇-水-冰醋酸(80201);流速1.0ml/min;检测波长254nm.结果大黄素浓度在25～250μg/ml范围内线性关系良好,r=0.99998.大黄素和氧化锌的平均回收率分别为100.22%和100.56%,RSD为0.87%和0.34%.结论本法操作简便,准确可靠,重现性好.     

儿童型消炎退热颗粒提取工艺实验研究

目的：研究儿童型消炎退热颗粒中靛玉红提取工艺的最佳条件。方法：采用L9（3^4)正交试验法。结果：实验设计的4因素中乙醇浓度对靛玉红含量有明显影响。结论：大青叶中靛玉红的最佳提取工艺条件是A3R1C2D2，考虑到原生产工艺虽不完善但临床应用有效的实际情况，该颗粒剂的最佳提取工艺是：用75%乙醇提取大青叶2次，每次1.5h，溶媒量为药材的6倍，接着，大青叶渣和其余药材一道用水提取2次，每次2h，加水量为药材的12倍。     

Toxicological studies on halopredone acetate

A toxicological study of 17,21-bis(acetyloxy)-2-bromo-6beta,9-difluoro-11beta-hydroxypregna-1,4-dien-3,20-dione(halopredone acetate; Topicon), a new topical antiinflammatory steroid, administered a laboratory animals, was carried out in order to evaluate the safety of this compound. Halopredone acetate compared favorably with either hydrocortisone acetate or potent synthetic corticoids in acute and subacute toxicity studies in mice and rats. This compound did not induce gastric ulcers in rats after repeated administration and did not have any teratogenic effect in rats and rabbits. Also, repeated daily application for 4 months to the skins of mice from two different strains was well-tolerated.     

Toxicological studies on azapropazone.

The toxicity of azapropazone, a non-steroidal anti-inflammatory analgesic compound was studied in mice, rats, hamsters, guinea pigs, rabbits, cats, mongrel and beagle dogs, domestic pigs, rhesus monkeys, cynomolgus monkeys and baboons in experiments which ranged from acute, single-dose studies up to treatment periods of 1 year. The beagle dog was found to be especially sensitive to gastro-intestinal ulceration from azapropazone and this contrasted with the marked lack of gastro-intestinal hazard to other animals and particularly to the 3 primate species studied. The animal experiments did not indicate any potential risk to other body systems, and comparisons with other anti-inflammatory compounds, where these were made, suggest that azapropazone is at least as safe as other commonly-used agents. Azapropazone was not found to have teratogenic, carcinogenic or antimitotic activity and was shown not to produce local tissue damage.     

Toxicological studies on floctafenine.

The toxicity of floctafenine, a nonnarcotic agent with analgesic properties, was investigated in mice, rats, rabbits, and dogs after single-dose oral or parenteral administration, and in rats and dogs after repeated oral administration for periods of 6 months. At high doses, floctafenin induced gastric lesions, particularly in dogs. It has a safety margin between active dose and toxic dose much wider than that of aspirin or indomethacin. High doses administered to pregnant mice, rats, and rabbits had some embryotoxic, but no teratogenic activity.     

Toxicological studies on 2,4,6-tribromoanisole

TBA, or 2,4,6-tribromoanisole, is a musty-smelling metabolite of 2,4,6-tribromophenol that is used as a flame retardant and an antifungal agent for wooden pallets and packaging materials. The compound can impart its peculiar, often offensive, odor on product packaging to the concern of consumers for the safety of the package contents. These studies were conducted to evaluate the safety of TBA to humans ingesting products tainted with TBA. In addition to the 28-day oral study, a bacterial reverse mutation study was conducted, and confirmed that TBA was not mutagenic. To evaluate oral safety, TBA was evaluated in single dose and 5-day and 4-week repeated dose oral toxicity studies in rats. The test article, administered in single gavage doses of 2000, 5000 and 7500 mg/kg body weight (bw), in 5 daily repeated doses of 1000, 2000 or 3000 mg/kg bw/day or in 28 daily oral gavage doses of 0 001, 0.01, 100, and 1000 mg/kg bw/day did not result in any deaths. Also, the single and repeat dose studies resulted in no significant differences between control and treated groups on body weight gain, food consumption, clinical observations, blood biochemical values, and hematology findings. Treatment-related adverse findings were only detected in male rats during repeated dose studies and were associated with high plasma concentrations of TBA. The test article-related finding of hyaline droplets in the cortical tubular epithelium of kidneys was associated with increases in α_2μ-globulin content in the kidneys as indicated by the intensity of immunohistochemical staining. These findings were correlated with an increased weight of kidneys in males administered 1000 mg/kg bw/day for 28 days. Chemical induction of hyaline droplets containing α_2μ-globulin in the renal proximal tubule is a process unique to the male rat and is not relevant for human risk assessment. Findings of increased liver weight with minimal centrilobular to diffuse hepatocellular hypertrophy in males treated with TBA at 1000 mg/kg bw/day for 28 days were considered to be an adaptive metabolic response to xenobiotic administration. The increased volume of urine, noted in both males and females treated with 1000 mg/kg bw/day was considered adaptive and necessary to excrete the high xenobiotic burden resulting from TBA administration. TBA appeared to be highly bioavailable since high concentrations of TBA were detected in plasma, at 1, 4 and 8 h after administration of TBA at 100 and 1000 mg/kg bw for 1 and 28 days. Levels were dose-related but did not clarify the course of TBA elimination with time after administration. These studies indicate that TBA, administered orally to rats, produced male rat-specific, treatment-related toxicity at the highest orally administered dose in repeated dose (5-day at 3000 mg/kg bw and 28-day at 1000 mg/kg bw) studies. Therefore, the NOAEL for the 28-day oral study was determined to be 1000 mg/kg bw/day for the rat.     

Toxicological and teratological studies of azanidazole

A toxicological study of 4-[(E)-2-(1-methyl-5-nitro-1H-imidazol-2-yl)-ethenyl]-2-pyrimidinamine (azanidazole, Triclose), a new systemic antitrichomonas agent, was run in laboratory animals in order to evaluate its safety. Studies also covered the effects of azanidazole on reproduction in rats treated prior to mating, on fetal development in rabbits and rats, and on fetal survival and growth in rats treated with it during late pregnancy and lactation. The obtained data indicate that azanidazole was well tolerated when administered as a single dose or repeated daily doses for six months. Furthermore, no adverse reproductive effects and no evidence of teratogenic activity were observed in all of the tested animal species. Survival indices were not affected, and body weight of progeny was normal in all studies on reproduction and peri- and post-natal toxicity.     

Toxicological studies on cefuroxime sodium.

The intravenous LD50 of cefuroxime sodium for mice was 10.4 g/kg. The maximum dosage administered in other acute toxicity tests was well tolerated by mice (10 g/kg, subcutaneous), by rats (4 g/kg, intravenous, 5 g/kg, subcutaneous) and by cats, dogs and monkeys (2 g/kg, intramuscularly). Cefuroxime sodium was administered subcutaneously (s.c.) or intramuscularly (i.m.) for 3 months to rats (100, 300 or 900 mg/kg/day) followed by a recovery period, and also for 6 months to rats and dogs (50, 150 or 450 mg/kg/day) and for 1 month to monkeys (150 or 450 mg/kg/day). In all these tests there were no serious toxic effects. Minor haematological changes were attributable in part if not entirely to haemorrhage and tissue reaction at the site of injection of large doses. In rats large doses caused some increase in urine volume and electrolyte excretion, and slightly aggravated an age related nephropathy. Administration to rats intravenously (i.v.) for1 month of up to 400 mg/kg/day had no toxic effects. In reproduction studies on mice and rabbits there were no adverse effects on fertility, organogenesis or the rearing of young.     

Toxicological studies of the antimicrobial alkaloid juliflorine

Juliflorine (CAS 76202-00-1), an antibacterial and antidermatophytic alkaloid, was studied for its toxicity in mice, rabbits, chicks, chick embryos and vero cell line. In mice LD50 was determined as 17.46 mg/kg; in rabbits 33.11 mg/kg; in chicks 24.104 mg/kg; and in chick embryos 18.284 mg/kg. In rabbits the subsequent injection of non-lethal doses (10 mg/kg) through the same route induced swelling, induration, inflammation and tissue degeneration (around injected area) with sterile abcess. Topically 1% juliflorine in polyethylene glycol-400 was found non-toxic but higher concentrations (less than or equal to 2.5%) produced irritation and inflammation; the severity of reaction was dose dependent. In vero cell-line juliflorine was found toxic at greater than or equal to 5 micrograms/ml. Injected animals showed lethargy, prostration and weakness (dose dependent) but recovered after some days, while newly hatched chicks, from injected eggs with higher dose, did not survive after 48 to 72 h. Internal organs (liver, kidney, brain, etc.) of dead, sick and healthy animals were examined and found normal.     

Toxicological studies of liver cells by microspectrofluorometry

The kinetics of xenobiotic biotransformation was measured in single, isolated, perifused liver cells with microspectrofluorometry using highly sensitive photodetection systems. A program of further applications to biochemical toxicological problems is presented.Part of Dissertation of these authors