人乳头状瘤病毒与宫颈癌生物学行为的相关性研究

目的探讨高危型人乳头状瘤病毒（HPV）DNA与宫颈癌生物学行为的相关性。方法对66例宫颈癌患者及103例宫颈癌行手术治疗后随访病例宫颈脱落细胞,采用第二代杂交捕获技术行高危型HPV DNA检测。结果①66例中62例HPV DNA检测阳性,阳性率达94％,仅4例阴性（6％）。②宫颈癌患者各临床分期、病理分级间HPV DNA阳性率差异无统计学意义,但发生宫颈间质侵入的宫颈癌患者HPV阳性率及病毒DNA载量均明显上升,差异有统计学意义（P〈0．05）。③96％（99／103）的宫颈癌经手术治疗后患者HPV DNA检测为阴性。结论高危型HPV感染可能与宫颈癌血管生成、浸润和转移有关;第二代杂交捕获技术为高效的宫颈癌筛查方法。     

非放射性标记核酸杂交检测宫颈癌等组织中人乳头状瘤病毒的感染(简讯)

人乳头状瘤病毒(Human popilloma viruses简称HPV)的某些类型与宫颈癌疾病发病有关,已为国内外所重视。本文选用HPV 6、11、16、18型分别用非放射性标记化合物Digoxin标记病毒DNA作探针,通过斑点杂交和Southern转移杂交检测宫颈癌等活检组织及其对应的阴道脱落细胞各122     

HPV E6/E7 mRNA检测在宫颈癌筛查中的应用

目的通过检测同一宫颈上皮内癌变或宫颈癌疑似病例宫颈石蜡包埋样本及脱落细胞中HPV E6/E7 m RNA的水平,分析两种检测结果的差异,探讨二者在宫颈癌筛查的应用价值。方法对302例宫颈上皮内癌变或宫颈癌疑似病例分别取宫颈活检组织及脱落细胞,两类标本均采用b DNA技术进行HPV E6/E7 m RNA检测,同时行病理学检查,并对检测的阳性率及灵敏度、特异度做出统计学分析。结果石蜡包埋组织检测HPV E6/E7 m RNA的阳性率为48.7%,高于脱落细胞的阳性率40.1%(χ2=4.192,P<0.05)。石蜡包埋组织检测HPV E6/E7m RNA对诊断的灵敏度为75.0%,高于脱落细胞的61.4%(χ2=5.046,P<0.05);石蜡包埋组织检测HPV E6/E7 m RNA对诊断的特异度为81.2%,脱落细胞为77.1%,二者差异无统计学意义(χ2=0.641,P>0.05)。结论检测石蜡包埋样本中的HPV E6/E7 m RNA,可更加精确预测宫颈病变进展风险,对宫颈癌筛查工作具有更好的临床意义。     

应用聚合酶链反应方法检测宫颈癌患者的人乳头瘤病毒

目的探讨人乳头瘤病毒（human papillomavirus,HPV）在宫颈癌的发生中的作用。方法采用聚合酶链反应（PCR）-核酸内切酶分型检测宫颈癌活检组织中HPV-DNA,对来源于子宫颈癌患者的石蜡包埋组织标本进行四种常见生殖道人乳头瘤病毒（HPV）（HPV6、11、16、18）的检测和分型。结果在宫颈癌活检组织中HPV-16,18型39.1%,与正常妇女宫颈组织阳性率均为2.2%比较,差异均具有统计学意义。尖锐湿疣主要是HPV-Ⅰ（6,11型）感染,阳性率为69.2%,HPV-Ⅱ仅占4.2%。结论宫颈癌主要与HPV16及18型感染有关。     

尖锐湿疣患者HPV基因分型检测及临床意义

目的探讨外阴尖锐湿疣患者高危型HPV感染,及早防治宫颈癌。方法选取2012年5月-2013年6月到我院皮肤性病科接受治疗的患有外阴尖锐湿疣的女性患者106例,同时选取同一时期接受筛查的无尖锐湿疣女性患者116例,避开患者月经期,用取样刷刷取宫颈脱落细胞或疣体上皮细胞,选用广州安必平公司生产的人乳头瘤病毒分型检测试剂盒（PCR-反向斑点杂交法）进行检测,标本采集后立即送检病理科,严格按照试剂盒说明进行操作。结果 CA实验组低危型HPV感染阳性率93.4%。其中重叠高危型HPV（16、18、53、35、58等）感染者为64.1%。筛查组低危型HPV总感染率18.9%,重叠高危型占12.9%,尖锐湿疣组高危型HPV感染率明显高于对照组比较差异具有统计学意义（P〈0.01）。结论在对尖锐湿疣病患进行临床治疗时,同时对其进行高危型的HPV病毒基因分型检测,能够实现早期预防子宫颈癌的作用,值得在实际临床中进行推广。     

高危型人类乳头瘤病毒的检测在宫颈癌筛查中的应用

目的探讨高危型人类乳头瘤病毒（HPV）检测在宫颈癌筛查中的应用价值。方法收集328例门诊就诊患者的资料,同时做宫颈组织活检及杂交捕获二代实验检测高危型HPV,以宫颈组织活检结果为标准,分析高危型HPV的检测结果。结果宫颈组织活检结果分别为宫颈炎、CINⅠ、CINⅡ、CINⅢ及浸润癌的高危型HPV感染率分别为20．9％、57．6％、90．6％、96．5％和100％;CINⅡ级以上病变患者高危型HPV平均感染率为94．3％,与宫颈炎感染率20．9％相比,差异有统计学意义。结论CIN病变程度越严重,高危型HPV感染率越高;高危型HPV感染在宫颈癌早期病变的筛查中具有风险提示作用。     

宫颈癌患者HPV16感染及其血清抗体检测分析

目的：探讨宫颈癌与人乳头瘤病毒16（HPV16）感染及其血清抗体的关系，为HPV感染及宫颈癌的临床诊断提供理论依据。方法：选择28例宫颈癌患，用PCR方法检测宫颈癌组织的HPV型别；并采用重组杆状病毒－昆虫细胞系统制备HPV16病毒样颗粒（VLPs），用ELISA方法检测其血清HPV16VPLs抗体，同时以36份尖锐湿疣患血清及72份健康体检查血清作为对照。结果：宫颈癌HPV通用型DNA阳性率为50％（14／28），HPV16DNA阳性率为42．9％（12／28），HPV18DNA阳性率为3．5％（1／28），较健康对照组阳性率为1．4％（1／72）、中位数－0．0220）－0．0265）和尖锐湿疣组阳性率为8．3％（3／36）、中位数为0．0165（0．0145）差异具显性意义（P＜0．01）。HPV16DNA检测法与HPV16VLPsELISA血清抗体检测法两间呈中度相关（K＝0．471，P＜0．05）。结论：本组宫颈癌以HPV16感染为多见，HPV16VLPs血清ELISA抗体检测可用作HPV16感染的血清学诊断和宫颈癌的免疫学研究。     

女性宫颈HPV基因检测及分析

目的研究女性宫颈常见人乳头瘤病毒（HPV）感染情况,探讨HPV的危害及处理办法。方法对620例患有宫颈炎、宫颈炎合并阴道炎、阴道炎及尖锐湿疣的门诊女性取宫颈脱落细胞进行HPV基因分型检测,并对HPV亚型感染的阳性率进行对比分析。结果620例标本中,HPV阳性者238例（38．4％）,其中单一HPV阳性者195例（31．5％）,复合HPV阳性者43例（6．9％）。涉及感染的HPV有23个亚型,常见的有HPV16、HPV11、HPV58、HPV6、HPV56、HFV43等,其中HPV16阳性率与其他亚型相比,差异有统计学意义;四组疾病中宫颈炎组与阴道炎组、尖锐湿疣组相比HPV16阳性率差异均有统计学意义,而与宫颈炎合并阴道炎组相比,HPV16阳性率差异无统计学意义;尖锐湿疣组与其他三组相比,HPV11型阳性率差异有统计学意义。结论HPV16为门诊女性尤其患宫颈炎女性最常见感染,而HPVI1为尖锐湿疣女性患者最常见感染,对HPV进行基因分型检测可能有助于宫颈癌及尖锐湿疣的预防、治疗和预后的判断。     

高危型HPV检测联合液基细胞学在宫颈癌前病变筛查中的应用

目的:探讨高危型人乳头瘤病毒(HPV)检测联合液基细胞学检测(LCT)在宫颈癌前病变筛查中的应用。方法:对18848例门诊受检者的宫颈脱落细胞标本采用液基细胞技术进行检测,细胞学异常(ASCUS及以上病变)的1691例患者的宫颈脱落细胞标本采用美国Digene公司杂交捕获仪(HC2)检测13种高危型HPV病毒的感染情况。结果:18848例LCT受检者,共发现异常1691例(不包括普通炎症改变)占8.97%,其中ASCUS1044例、LISL416例、HISL192例、SCC39例,分别占5.54%、2.21%、1.02%、0.21%。1691例细胞学异常的受检者中高危型HPV阳性1072例,阳性率63.39%;其中AUSCS、LISL、HISL高危型HPV阳性率分别为53.83%、70.43%、93.23%,显示癌前病变与高危型HPV感染密切相关。结论:高危型HPV检测联合液基细胞学检测能及时有效地发现异常细胞和宫颈癌的诱因,是一种合理而有效的宫颈癌筛查方案。     

三种方法诊断尖锐湿疣的对比研究

目的探讨原位分子杂交法诊断尖锐湿疣的敏感性与特异性，并与组织病理及醋白试验相比较。方法应用原位分子杂交法[所用探针为地高辛标记的DNA探针（HPV6／11，HPVl6／18，HPV31／33）]、组织病理及醋白试验分别检测实验组和对照组标本。结果37例标本中，醋白试验34例阳性；组织病理检查37例均阳性；原位分子杂交法的总阳性率为94．6％，HPV6／11阳性31例（阳性率83．7％），HPV16／18阳性2例（阳性率5．4％），1例标本HPV6／11及HPVl6／18均阳性（2．7％），HPV31／33阳性1例（阳性率2．7％），阳性标本可见细胞核着蓝色，阳性反应物主要分布在棘层空泡化细胞的细胞核内。结论原位分子杂交法检测HPV敏感性高，特异性强，能对感染有明确的组织学定位，而且还能进一步鉴定感染的型别，因此是诊断和研究尖锐湿疣的较好和先进方法。     

DNA sequences of human papillomavirus types 11, 16, and 18 in lesions of the uterine cervix in the west of Scotland

Punch biopsy specimens of the cervix were examined both histologically and for the presence of human papillomavirus (HPV) DNA sequences. The presence of HPV DNA sequences was sought with the Southern blot technique using radioactively labelled HPV-6, 11, 16, and 18 DNA probes, both together and separately. Twenty six biopsy specimens were examined. Histological examination showed cervical intraepithelial neoplasia grade 2 or 3 in 16 specimens, viral changes (koilocytosis) in four, and inflammation or a normal appearance in three. Eleven specimens were negative for HPV DNA sequences, 10 contained HPV-16 DNA, four contained HPV-18 DNA, and one contained both HPV-18 and HPV-11 DNA. Episomal HPV-16 DNA was detected in one case of cervical intraepithelial neoplasia grade 3 and in five cases of cervical intraepithelial neoplasia grade 2/3 with koilocytosis; and episomal HPV-18 DNA was found in two specimens classed as cervical intraepithelial neoplasia grade 2/3, one of which also contained HPV-11 DNA, and in one specimen that showed viral changes alone. Integrated HPV DNA was found in six specimens (four with HPV-16 DNA and two with HPV-18 DNA), including two cases of chronically inflamed cervix with no histological evidence of viral infection or cervical intraepithelial neoplasia. Detection of viral DNA in early lesions may identify patients at risk of malignant progression. This is the first report of HPV-18 DNA in cervical intraepithelial neoplasia in Scotland.     

Correlation between physical status of human papilloma virus and cervical carcinogenesis

The prevalence of human papilloma virus (HPV)-16 in patients with cervical cancer,the physical status of HPV-16 in patients with cervical lesions,and the role of HPV-16 integration in cervi-cal carcinogenesis were investigated.HPV genotyping was performed by using PCR approach with the primer GP5+/GP6+ and type-specific primer on biopsy specimens taken operatively from 198 women.Multiple PCR was done to detect physical status of HPV-16 in a series of cervical liquid-based cytology samples and biopsy specimens obtained from different cervical lesions with HPV-16 infection,includ-ing 112 specimens with cervical cancer,151 specimens with CINⅠ,246 specimens with CINⅡ and 120 specimens with CINⅢ.The results showed that there were 112 cervical cancer samples (56.57% of total cervical cancer patients) with HPV-16 infection.The frequency of HPV-16 pure integration was 65.18% (73/112),56.57% (47/120),23.58% (58/246) and 7.95% (12/151) in cervical cancer,CINⅢ,CINⅡand CINⅠ patients respectively.In situ hybridization was performed on some paraffin-embedded sections of CINⅡ,CINⅢ and cervical cancer to verify the physical status of HPV-16 infection.Sig-nificant difference was observed between cervical cancer and CINⅠ,CINⅡ,CINⅢ in the frequency of HPV-16 integration (P<0.01).It is suggested that HPV-16 is the most prevalent type and is associated with cervical cancer.In the case of HPV-16 infection there are close associations between the severity of cervical lesions and the frequency of HPV-16 integration.The application of testing HPV genotyping and physical status based on detection of HC-Ⅱ HPV DNA would be in favor of predicting the progno-sis of cervical precancerosis and enhancing the screening accuracy of cervical cancer.     

The association between cervical carcinoma and human papilloma virus (HPV) in Xiangyuan County.

A mass survey was conducted to investigate the association between cervical carcinoma and human papilloma virus (HPV) infection in a high-risk area, Xiangyuan County, Shanxi Province. Fifty-four cases of cervical cancer in situ (CIS), including severe dysplasia (CIN III), 14 cases of invasive cervical carcinoma, 28 cases of mild cervical dysplasia (CIN I), and 13 cases of moderate cervical dysplasia (CIN II) were identified among 6710 women examined. One hundred and sixty-nine punch biopsies from abnormal cervix and genital tract were examined for the presence of HPV 6B/11, 16 and 18 DNA sequences by dot blot hybridization: The positive rates of HPV infection in cervicitis, CIN I, II, III, CIS and invasive cervical carcinoma specimens were 35.06% (27/77), 25% (2/8), 33.33% (2/6), 40% (2/5), 70.58% (24/34) and 40% (4/10), respectively. Among these groups, there was no regular distribution of HPV types except for HPV 16, the positive rate of which was increased from 25% in CIN I to 46.15% in CIS: HPV 16 was also the only type seen in the invasive cervical cancer group. Distribution analysis of HPV types in HPV-positive cervical cancer tissues uncovered an HPV 16 positive rate of 59.4%; 3.7 and 2.4 times higher than those of HPV 6B/11 and HPV 18, respectively. These data confirm the close association between cervical cancer and HPV 16 infection.     

广西地区子宫颈癌发病与人乳头瘤病毒感染关系的研究 Ⅱ.人乳头瘤病毒感染与宫颈癌组织病理形态学的关系

对54例广西地区宫颈癌组织活检标本进行HPV 16型基因组相关序列检测及病理切片检查,发现:(1)13例HPV 16型和3例HPV 11/6型均为鳞癌,6例HPV R型中有4例也为鳞癌。16型的13例中有8例为低分化癌,R型的6例中有5例也为低分化癌。(2)不同型别HPV感染的宫颈癌组织,其病理形态学待征有所不同。(3)Southern blot核酸杂交技术和病理形态学检查,二种方法判断HPV感染及其型别,结果是基本一致的。     

原位杂交检测宫颈鳞癌HPV感染意义的进一步探讨

目的 :从分子水平了解宫颈鳞癌人类乳头状瘤病毒 (humanpapillomavirus ,HPV)感染情况及放化疗对其的影响。方法 :用原位杂交 (insituhybridization ,ISH)技术检测宫颈鳞癌术前 (活检 ,未经任何治疗 )和术后 (经放疗和 /或化疗后 )标本HPV 4个亚型 ( 16/18/11/6)感染的表达。结果 :术前活检标本表达总阳性数 5 2 /60 ,总阳性率 86.7% ;术后 47/60 ,总阳性率 78.3 %。术前HPV16表达率 84.0 % ,术后HPV16表达率为 78.8%。结论 :宫颈鳞癌的发生发展与HPV感染关系密切 ;术前术后即治疗前后HPV感染无差异性     

新疆喀什11个团场5199名女性人乳头状瘤病毒感染情况分析

目的 探讨新疆喀什11个团场地区女性人乳头瘤病毒(human papilloma virus，HPV)阳性感染情况，为少数民族地区宫颈癌防控策略提供参考依据。方法 选取2020年3月至6月5 199例女性的宫颈上皮脱落细胞标本，采用基因扩增及导流杂交技术对14 种HPV亚型进行分型检测。结果 共检出HPV阳性样本554例，阳性率10.66%，其中单一感染占比5.04% ，多重感染占比1.90%，单一感染率高于多重感染率。维吾尔族女性的HPV总感染率与HPV-16/18感染率均显著高于汉族女性。30~39岁年龄段HPV检出率最高，其次为60~69岁，检出率分别为13.61%、10.63%。结论 新疆喀什11个团场地区女性中年和老年妇女HPV 感染率偏高，维吾尔族女性较汉族女性更易感HPV病毒，且单一型、HPV-16和HPV-18基因亚型多见，预防接种应选用包含这两种亚型的疫苗。     

Histological and cytological evidence of viral infection and human papillomavirus type 16 DNA sequences in cervical intraepithelial neoplasia and normal tissue in the west of Scotland: evaluation of treatment policy

Biopsy samples from 27 patients referred to a colposcopy clinic in Glasgow for cervical abnormalities were assessed for the relations among colposcopic appearances, cytological and histological diagnosis, expression of papillomavirus antigen, and the presence of human papillomavirus (HPV) types 6, 11, 16, and 18 deoxyribonucleic acid (DNA) sequences. Specimens were from colposcopically abnormal areas of the transformation zone and from colposcopically apparently normal areas of the zone in the same patients (paired matched internal control tissue). All 27 women referred for abnormal smears had colposcopic abnormalities. HPV-16 or 18 DNA sequences were detected in 20 of the 27 colposcopically abnormal biopsy samples and 13 of the 27 paired normal samples. Twelve samples of colposcopically normal tissue contained histological evidence of viral infection but only four of these contained HPV DNA sequences. The other nine samples of colposcopically normal tissue which contained HPV DNA sequences were, however, histologically apparently normal. HPV-6 and 11 were not detected. Integration of the HPV-16 genome into the host chromosome was indicated in both cervical intraepithelial neoplasia and control tissues. In two thirds of the HPV DNA positive samples the histological grade was classed as normal, viral atypia, or cervical intraepithelial neoplasia grade 1. Papillomavirus antigen was detected in only six of the abnormal and three of the normal biopsy samples, and HPV DNA was detected in all of these. The detection of HPV DNA correlates well with a combination of histological and cytological evidence of viral infection (20 of 22 cases in this series). A poor correlation between the site on the cervix of histologically confirmed colposcopic abnormality and the presence of HPV DNA sequences implies that a cofactor other than HPV is required for preneoplastic disease to develop. A separate study in two further sets of biopsy samples examined the state of HPV DNA alone. The sets were (a) 43 samples from cervical intraepithelial neoplasia and nine external controls and (b) 155 samples from cervical intraepithelial neoplasia, cervical cancer, vulval intraepithelial neoplasia, and vulval cancer and external controls. HPV-11 was found in only two (4.7%) of the 43 specimens from cervical intraepithelial neoplasia, whereas HPV-16 was found in 90 (58%) of the other 155 specimens. These results also suggest that HPV subtype is subject to geographical location rather than being an indicator of severity of the lesion or of prognosis.     

用生物素标记的DNA探针检测尖锐湿疣和宫颈癌中的人类乳头瘤病毒DNA序列

用生物素标记的HPV6/11DNA和HPV16/18DNA探针,对尖锐湿疣和宫颈癌标本进行原位杂交。结果大多数尖锐湿疣含HPV6/11DNA,所以尖锐湿疣的主要致病因子是H2V6/11.HPV6/11DNA阳性细胞主要位于上皮的表层,少数位于深层。个别合并HPV16/18感染,或仅为HPV16/18所感染的病人恶变的可能性大。大多数宫颈癌病人含HPV16/18DNA,提示宫颈癌的主要致病因子为HPV16/18。一些病人合并HPV6/11感染。在未找见HPV16/18DNA的病例中,可能存在其它致癌因子。     

用生物素标记的DNA探针检测尖锐湿疣和宫颈癌中的人类乳头瘤病毒DNA序列

用生物素标记的HPV6/11DNA和HPV16/18DNA探针,对尖锐湿疣和宫颈癌标本进行原位杂交。结果大多数尖锐湿疣含HPV6/11DNA,所以尖锐湿疣的主要致病因子是H2V6/11.HPV6/11DNA阳性细胞主要位于上皮的表层,少数位于深层。个别合并HPV16/18感染,或仅为HPV16/18所感染的病人恶变的可能性大。大多数宫颈癌病人含HPV16/18DNA,提示宫颈癌的主要致病因子为HPV16/18。一些病人合并HPV6/11感染。在未找见HPV16/18DNA的病例中,可能存在其它致癌因子。     

Folate deficiency and cervical dysplasia.

OBJECTIVE--To test the hypothesis that nutritional deficiency affects the incidence of cervical dysplasia in young women. DESIGN AND SETTING--Case-control study. Participants were derived from community family-planning clinics and referrals to a colposcopy center. PARTICIPANTS--A total of 726 subjects were screened, yielding 294 cases of dysplasia and 170 controls defined by coexistent cytologic and colposcopic evidence. MAIN OUTCOME MEASURES--Planned prior to data collection. Odds ratios were computed using logistic regression models to evaluate association between cervical dysplasia and sociodemographic, sexual, and reproductive factors; smoking; oral contraceptive use; human papillomavirus (HPV) infection; and 12 nutritional indices determined by blind analysis of nonfasting blood specimens. RESULTS--The number of sexual partners, parity, oral contraceptive use, and HPV-16 infection were significantly associated with cervical dysplasia. Plasma nutrient levels were generally not associated with risk. However, red blood cell folate levels at or below 660 nmol/L interacted with HPV-16 infection. The adjusted odds ratio for HPV-16 was 1.1 among women with folate levels above 660 nmol/L but 5.1 (95% confidence interval, 2.3 to 11) among women with lower levels. Interactions of red blood cell folate levels with cigarette smoking and parity were also present but were not statistically significant. CONCLUSION--Low red blood cell folate levels enhance the effect of other risk factors for cervical dysplasia and, in particular, that of HPV-16 infection.