二色桌片参的化学成分研究Ⅰ.二色桌片参的化学成分分析

对海参动物－二色桌片参［Ｍｅｎｓａｍａｒｉａ ｉｎｔｅｒｃｅｄｅｎｓ］（Ｌａｍｐｅｒｔ）的主要化学成分的分析结果表明,这种海参干品蛋白含量高达８５．９８％,维生素Ａ、Ｅ含量也较高,有较高的营养价值。更重要的这种海参还含有丰富的活性蛋白多糖、多糖和皂甙类物质,是开发海洋药物的丰富资源。     

二色桌片参皂苷intercedenside A的抗真菌和抗肿瘤活性

二色桌片参 (Mensamaria intercedens Lampert)属枝手目 (Dendrchirotida)瓜参科(Cucumariidae)棘皮动物[1],从我国福建东山到海南岛的海域均有分布,资源十分丰富.有研究机构对该种海参体内多糖和糖蛋白的分离纯化、化学特征和药理活性进行了研究[2],有关该种海参体内皂苷成分的研究也有报道[3].本文报道从二色桌片参体内分得的海参皂苷intercedenside A的抗真菌和抗肿瘤活性.     

二色桌片参的化学成分研究Ⅳ二色桌片参糖蛋白的分离性质与抗肿瘤 …

二色桌片参「Ｍｅｎｓａｍａｒｉａ ｉｎｔｅｒｃｅｄｅｎｓ（Ｌａｍｐｒｔ）」的体壁经水煮提、分离、最后经Ｓｅｐｈａｄｅｘ Ｇ－２００柱层析纯化得二色桌片参糖蛋白Ｉ（ＧＰＭＩ－Ｉ）。ＧＰＭＩ－Ｉ经酶解后得二色桌片参糖蛋Ⅱ（ＧＰＭＩ－Ⅱ）。分别研究了ＧＰＭＩ－Ｉ和ＧＰＭＩ－Ⅱ的性状、化学组成和抗肿瘤活性,结果表明：ＧＰＭＩ－Ｉ和ＧＭＰＩ－Ⅱ都是糖蛋白,但蛋白组成和糖含量明显不同。ＧＰＭＩ－Ⅱ对小鼠Ｓ１８     

二色桌片参的化学成分研究Ⅱ二色桌片参多糖-1，一种新的岩藻聚糖

利用乙醇沉淀分级技术从二色桌片参双酶水解液里分离到一种纯的活性多糖,定名PMI-1。用高效薄层层析法鉴定其组成的单糖,发现PMI-1只含有L-岩藻糖。根据其分析结果和理化特性,PMI－1应是由若藻糖基和岩藻糖-4硫酸酯基组成的均多糖。研究PMI－1的核磁共振谱,可认定它是一种具有直链的和α1－α联接的均多糖。因此它是一种新的岩藻聚糖。     

二色桌片参的化学成分研究Ⅳ二色桌片参糖蛋白的分离性质与抗肿瘤活性的研究

二色桌片参〔Mensamaria intercedens( L ampert)〕的体壁经水煮提、分离、最后经 Sephadex G- 2 0 0柱层析纯化得二色桌片参糖蛋白 ( GPMI- )。GPMI- 经酶解后得二色桌片参糖蛋白 ( GPMI- )。分别研究了GPMI- 和 GPMI- 的性状、化学组成和抗肿瘤活性 ,结果表明 :GPMI- 和 GPMI- 都是糖蛋白 ,但蛋白组成和糖含量明显不同。GPMI- 对小鼠 S180 肉瘤有明显抑制作用 ,而无直接毒性 ,抑瘤率为 4 8.8%。而 GPMI- 却能显著提高 S180 肉瘤荷瘤小鼠的脾指数 ,呈免疫增强作用。     

二色桌片参的化学成分的研究(V)二色桌片参糖蛋白抗肿瘤活性的研究

本文探讨二色桌片参糖蛋白 GPMI- 和 GPMI- 对小鼠移植性肿瘤 S180 的抑制作用以及对荷瘤小鼠肝脾等器官重量的影响。实验结果显示 ,经蛋白酶水解后的糖蛋白 GPMI- 在剂量 2 0μg· g-1时小鼠肉瘤S180 有较显著的抑制作用 (P<0 .0 5 ) ,抑瘤率达 4 8.8%。同时发现 ,GPMI- 在 30μg· g-1剂量下可显著增加荷瘤小鼠的脾指数 (P<0 .0 5 ) ,GPMI- 在 4 0 μg· g-1剂量下能够显著降低荷瘤小鼠肝指数 (P<0 .0 5 )。     

二色桌片参的化学成分研究：Ⅱ 二色桌片参多糖—1,一种新的岩 …

利用乙醇沉淀分级技术从二色桌片参双酶水争液里分离到一种纯的活性多糖,定名ＰＭＩ－１。用高效薄层导析法鉴定其组成的单糖,发现ＰＭＩ－１只含有Ｌ－岩藻糖。根据其分析结果和理化特性,ＰＭＩ－１应是由岩藻糖基和岩藻糖－４硫酸酯基组成的均多糖。研究ＰＭＩ－１的核磁共振谱,可认定它是一种具有直链的和α１－α联接的均多糖。因此它是一种新的岩藻聚糖。     

黄豆苷元片治疗心绞痛的临床观察

心绞痛是严重危害中老年健康的常见病，随着人们生活水平的提高，其发病率及死亡率逐年增高。我们从1998~2000年用黄豆苷元片对62例心绞痛病人进行治疗，并与硝苯砒啶(心痛定)进行治疗对照观察，结果表明黄豆苷元片治疗心绞痛取得满意疗效，现报道如下。1研究对象与方法1.1研究对象62例病人均为1998-04~2000-04我院收治的心绞痛病人，符合WHO关于心绞痛的诊断标准，至少有3个月以上典型劳力型心绞痛病史，每周发作至少数次，经休息或舌下含服硝酸甘油可缓解症状的为研究对象。62例心绞痛病人中，男38例，女2例。随机分为两组，治疗组32例…     

Soulieoside O,a new cyclolanostane triterpenoid glycoside from Souliea vaginata

A new cyclolanostane triterpenoid glycoside, soulieoside O (1), together with 25-O-acetylcimigenol-3-O-β-d-xylopyranoside (2) and cimigenol-3-O-β-d-xylopyranoside (3), was isolated from the rhizomes of Souliea vaginata. Their structures were characterized by spectroscopic analysis and chemical methods. The new compound showed moderate inhibitory activity against three human cancer cell lines with IC₅₀ values of 9.3–22.5 μM.     

可疑翼手参中的一个新三萜皂苷

目的：寻找结构新颖的三萜皂苷类化合物。方法：采用多种色谱方法．对属于枝手目瓜参科的可疑翼手参体内成分进行了分离纯化；并采用多种光谱(IR．UV，ESI-MS，2D-NMR)和化学方法对所分离化合物的结构进行了确定。结果与结论：得到一个新的三萜皂苷类化合物，我们把该化合物命名为coloehiroside A(1)。     

玉足海参中一个新的三萜皂苷脱硫衍生物

目的分离并鉴定玉足海参体内皂苷类化合物的结构。方法将自玉足海参体内提取得到的皂苷leu-cospilotaside A溶于吡啶∶二氧六环(1∶1)的混合液中。120℃加热3h,应用多种色谱技术对回流产物进行分离纯化,根据化合物的理化性质和波谱数据鉴定其结构。结果分离并鉴定了1个三萜皂苷类化合物:3-O-{-βD-吡喃奎诺糖(1(2)-β-D-吡喃木糖}-海参烷-9-烯-22-酮-3β,12α,17α,25α-四醇。结论玉足海参中提取的三萜皂苷leucospilotaside A脱硫衍生物为新化合物。     

Lecanorosides A and B,two new triterpene glycosides from the sea cucumber Actinopyga lecanora

Two new sulphated triterpene glycosides, lecanorosides A (1) and B (2), along with the known compounds holothurins A (3), A₁ (4), and B (5), were isolated from the sea cucumber Actinopyga lecanora. Their structures were deduced from extensive spectral analysis (NMR and MS) and chemical evidence. Glycosides 1 and 4 showed marginal in vitro cytotoxicity against two human tumour cell lines.     

玄参根中的一个新三萜四糖苷(英文)

在中药玄参提取分离的过程中,分离得到1个新三萜四糖苷3β-O-(β-D-xylopyranosyl-(1→2)-[β-D-glucopyranosyl(1→4)-β-D-glucopyranosyl-(1→3)]-β-D-fucopyranosyl-11,13(18)diene-olean-23α,28 dio1(1)和3个已知的苯乙醇苷(2～4),化合物2、3均为首次从该属植物中分离得到。通过1D,2D NMR和HR-ESI-MS等光谱数据阐明了化合物1的结构。     

Hillasides A and B, two new cytotoxic triterpene glycosides from the sea cucumber Holothuria hilla Lesson

Two new triterpene glycosides, hillasides A (1) and B (2), were isolated from the sea cucumber H. hilla Lesson, together with one known glycoside holothuria B (3). Their structures were deduced by extensive spectral analysis and chemical evidences. The presence of conjugated double bonds [22E,24-diene] in the aglycone of 1 is a rare structural feature among sea cucumber glycosides. The two glycosides showed significant cytotoxicity against eight human tumour cell lines (A-549, MCF-7, IA9, CAKI-1, PC-3, KB, KB-VIN and HCT-8) with IC₅₀ in the range of 0.1-3.8 μg/ml.     

Phytolacacinoside A,a new triterpenoid saponin from Phytolacca acinosa Roxb

Phytolacacinoside A (1), a novel triterpenoid saponin, together with the seven known compounds, was isolated from 75% ethanol extract of the root of Phytolacca acinosa Roxb (Phytolaccaceae). Their structures were elucidated on the basis of analysis of spectroscopic data and physicochemical properties as 3-O-β-[(β-d-glucopyranosyl-(1 → 4)-O-β-d-xylopyranosyl)]-11β-methoxy-jaligonic acid 30-methyl ester 28-O-β-d-glucopyranoside (1), 3-O-β-[(β-d-glucopyranosyl-(1 → 4)-O-β-d-xylopyranosyl)]-jaligonic acid 30-methyl ester 28-O-β-d-glucopyranoside (2, esculentoside G), 3-O-β-[(β-d-glucopyranosyl-(1 → 4)-O-β-d-xylopyranosyl)]-jaligonic acid 30-methyl ester (3, phytolaccoside E), 3-O-β-d-xylopyranosyl-jaligonic acid 30-methyl ester (4, phytolaccoside B), hypaphorine (5), palmitic acid monoglyceride (6), β-sitosterol (7), and daucosterol (8).     

Hillasides A and B, two new cytotoxic triterpene glycosides from the sea cucumber Holothuria hilla Lesson

Two new triterpene glycosides, hillasides A (1) and B (2), were isolated from the sea cucumber H. hilla Lesson, together with one known glycoside holothuria B (3). Their structures were deduced by extensive spectral analysis and chemical evidences. The presence of conjugated double bonds [22E,24-diene] in the aglycone of 1 is a rare structural feature among sea cucumber glycosides. The two glycosides showed significant cytotoxicity against eight human tumour cell lines (A-549, MCF-7, IA9, CAKI-1, PC-3, KB, KB-VIN and HCT-8) with IC(50) in the range of 0.1-3.8 microg/ml.     

A new triterpenoid saponin from the stem of Akebia trifoliata var. australis

A new triterpene glycoside mutongsaponin F (1), together with five known saponins and two known lipids, was isolated from the 70% ethanol extract of the stems of Akebia trifoliata (Thunb.) Koidz. var. australis (Diels) Rehd. Their structures were elucidated on the basis of the spectroscopic analysis and physicochemical properties as 3-β-[(β-d-glucopyranosyl-(1 → 2)-O-[β-d-glucopyranosyl-(1 → 3)-O-]-α-l-arabinopyranosyl)oxy]-30-norolean-12-en-28-oic acid α-l-rhamnopyranosyl-(1 → 4)-O-β-d-glucopyranosyl-(1 → 6)-O-β-d-glucopyranosyl ester (1), 3-β-[(β-d-glucopyranosyl-(1 → 2)-O-[β-d-glucopyranosyl-(1 → 3)-O-]-α-l-arabinopyranosyl)oxy]-30-norolean-12-en-28-oic acid (2), leonticin E (3), collinsonidin (4), arjunolic acid 28-O-glucopyranoside (5), asiatic acid 28-O-glucopyranoside (6), soya-cerebroside I (7), and 1-O-α-l-galactosyl-(1 → 6)-O-β-d-galactosyl-3-O-hexadecanoyl-glycerol (8), respectively.