Application of quantitative proteomics technologies to the biomarker discovery pipeline for multiple sclerosis

Multiple sclerosis is an inflammatory-mediated demyelinating disorder most prevalent in young Caucasian adults. The various clinical manifestations of the disease present several challenges in the clinic in terms of diagnosis, monitoring disease progression and response to treatment. Advances in MS-based proteomic technologies have revolutionized the field of biomarker research and paved the way for the identification and validation of disease-specific markers. This review focuses on the novel candidates discovered by the application of quantitative proteomics to relevant disease-affected tissues in both the human context and within the animal model of the disease known as experimental autoimmune encephalomyelitis. The role of targeted MS approaches for biomarker validation studies, such as multiple reaction monitoring will also be discussed.     

数控设备的数据传输

随着计算机技术的不断进步,软盘作为主要的文件传输工具的时代已经过去。但是某些运行DOS或Windows操作系统的数控设备仍然需要使用软盘或其他移动存储媒体进行数控文件传输。通过对Windows操作系统对等网功能的拓展和微软Microsoft Network Client3．0forMS-DOS软件的灵活使用,解决上述类型数控设备数控文件传输的问题。     

嵌入式系统中I^2S总线数据通信的软件模拟

对I^2S总线规范进行了介绍,分析了I^2S总线时序;对以太网数字语音广播系统中的语音解码芯片MS6336的数据传输格式进行了分析,并给出了软件模拟实现I^2S总线完成MCU和MS6336间语音数据传输的详细过程。结果表明,软件模拟实现I^2S总线具有现实可行性。     

基于电容检测芯片的电容检测系统设计

针对电容式传感器研发过程中缺乏有效的微小电容检测仪器的问题,设计了一种基于电容检测芯片MS3110的电容式传感器检测系统,给出了系统的硬件设计以及单片机和上位机部分的软件设计,并对系统的检测精度进行了测试。结果证明,该系统具有较高的检测精度。     

网络计划在软件项目进度管理中的应用

本文论述了网络计划的基本原理,描述了软件项目进度管理的实现过程,介绍了小型网站规划项目各任务时间参数的计算方法。根据确定型网络图原理,给出了MS Project进度计划控制的具体步骤。     

一个与输出形式无关的报表打印程序

报表打印在管理信息系统(MIS)中一向是个很重要的模块,但对程序员而言,编写报表打印程序实现一个灵活的报表打印也很困难.文章介绍了一个利用OLE Automation实现的与输出形式无关的报表打印程序,经实际应用,该程序具有灵活、方便、操作简单等特点.     

基于MS Agent的语音识别与合成

语音合成与语音识别提供了全新的人机交互方式,在信息处理、教育与商务应用等方面已显示出了巨大的优势。本文分析了MsAgent的工作原理,论述了基于MSAgent的语音系统的开发步骤,给出了系统运行示意图。     

MS characterization of apheresis samples from rheumatoid arthritis patients for the improvement of immunoadsorption therapy - a pilot study

Identification of proteins from apheresis samples was performed by both SDS-PAGE and 2-D gel separation of eluted proteins from staphylococcal protein A-based immunoadsorption columns (Prosorba^®) followed by MS peptide mass fingerprinting and MS/MS peptide sequencing on a MALDI QIT TOF mass spectrometer. MS/MS peptide sequencing was performed in conjunction with a micro reversed phase HPLC configured with an online MALDI plate-spotting device. Apheresis treatment had been performed in three patients with longstanding therapy refractory rheumatoid arthritis. 2-D gels displayed ca. 500 spots representing proteins that were eluted from the Prosorba^® columns. From 54 gels, a total of 1256 protein spots had been picked and yielded in the identification of 56 non-redundant proteins without counting isoforms. Proteins from the eluates belong to five major groups comprising (i) immunoglobulins (IgG, IgA, IgM heavy and light chains; about 40% of the spots), (ii) proteins involved in coagulation, (iii) HDL/LDL-associated proteins, (iv) proteins from the complement system, and (v) acute phase proteins. MS analysis showed that the full-length C3 complement protein had been cleaved upon complement activation, presumably on the column, such that the anaphylatoxin C3a was produced and released during therapy. Our results are consistent with clinical observations on both patient responses to therapy and reported adverse events. For the first time, direct molecular information has become available to support mechanistic reasoning for the principle of function of staphylococcal protein A-based immunoadsorption therapy and for the explanation of adverse events. According to our results, removal and/or modulation of immune complexes together with complement activation can be regarded as the major events that are taking place during Prosorba^® therapy. In order to avoid complement activation and induction of an inflammatory cascade, we suggest the prevention of C3a anaphylatoxin-related reactions during immunoadsorption therapy.     

MS5534B在大气压力监测系统中的应用

提出了一种采用MS5534B数字式气压传感器监测大气压力的设计方案,分析了MS5534B的工作原理,介绍了基于MS5534B的大气压力监测系统的硬件及软件设计。实际应用表明,MS5534B具有测量精度高,响应速度快,功耗低等特点,满足大气压力监测系统的要求。     

基于MS Office的企业项目管理系统的设计

基于MS Office的企业项目管理应用的设计。利用MS Office的快速开发,快速部署以及低成本的特性,开发满足用户需要的特定环境的企业项目管理应用。     

一种新的海洛因毒源判别方法及其软件使用

应用气质联用(GC/MS)分析方法,获得云南省9个地区缴获的1148个海洛因毒品样品中海洛因、单乙酰吗啡和乙酰可待因3种组分(记为A、B、c)的含量.在对数据做了一维、三维分布情况观察之后,提出一种"等高图"毒源判别模式.建成以地区归属的毒品数据库,并绘制分别用组分A、B、C为Z轴表示的各地区等高图.选择有毒源代表性的5个边境地区的15个等高图基础模式,编制出判别未知样品的毒源鉴定程序软件.对软件的使用过程、判别能力、升级没想也作了说明.     

陶瓷加工监控MS/TP网络服务延迟性能分析

作为一种轻量级的单级单服务器多站点轮询服务系统,主从令牌网络协议适用于陶瓷加工过程监控这类低带宽要求的自动化场景.MS/TP网络通过排队论进行数学建模,并从网络性能参数以及服务类型等方面对其服务延迟进行了数值分析,同时在陶瓷加工监控系统中对该模型进行了评估,结果表明在负载不超过60%时,其偏差度约在10～20%间.实验结果表明,建模分析可用于合理优化MS/TP链路层参数,并指导MS/TP现场网络系统的设计工作.     

Proteomic analysis of the response of the human neutrophil-like cell line NB-4 after exposure to anthrax lethal toxin

We used 2‐D DIGE to analyze the early response of NB‐4 cells, a human promyelotic leukemia cell line, exposed to lethal toxin from Bacillus anthracis at the proteome level. After a 2 h exposure, cells were still viable and 43% of spots (n = 1042) showed a significant change in protein level. We identified 59 spots whose expression had changed significantly, and these reflected cytoskeleton damage, mitochondrial lysis and endoplasmic reticulum stress. Actin filament assembly was disrupted as evidenced by an increase in both actin subunits and phosphorylated cofilin, whilst levels of tropomyosin, tropomodulin and actin related protein 2/3 complex subunit decreased. Lower levels of ATP synthase subunits and mitochondrial inner membrane protein were identified as markers of mitochondrial lysis. Levels of various stress response proteins rose and, uniquely, levels of Ca²⁺ binding proteins such as translationally controlled tumor protein rose and hippocalcin‐like protein 1 decreased. This response may have mitigated effects brought about by mitochondrial lysis and endoplasmic reticulum stress, and delayed or prevented apoptosis in NB‐4 cells. These results resemble findings of similar proteomics studies in murine macrophages, although quantitative differences were observed.     

Proteomics of a conundrum: Thoughts on addressing the aetiology versus progression of multiple sclerosis

Currently in the field of multiple sclerosis (MS) research there is an ongoing debate concerning the cause of the disease. MS is widely considered to begin with an autoimmune dysregulation. The disease does have a prominent autoimmune component however this may be representative of a secondary effect. There is growing evidence that the disease may be initiated by an underlying degeneration of oligodendrocytes. In our viewpoint, we discuss the potential differences between the aetiology and progression of MS. For the most part, proteomic analysis has focused on the autoimmune component of the disease. We suggest that proteomic analysis should be applied to investigating oligodendrocyte degeneration. We discuss the potential of the cuprizone animal model of demyelination and its usefulness in understanding oligodendrocyte degeneration. Immune suppressive therapies are effective at reducing clinical symptoms and improving quality of life. However, a cure is still lacking and as such the disease does still progress. We suggest that if the initiating cause is poorly understood, then curing MS is unlikely.     

MS302多媒体软件功能记忆法

本文介绍了ＭＳ３０２多媒体软件（生理学专用）基于功能的记忆法，为例于学生快速而准确地记忆，普及现代化的多媒体教学手段，提高实验课堂教学效果，提高教学质量，深化实验教学改革提供资料。     

NET Remoting技术在智能布置系统中的应用

本文分析了MS.NET Remoting的体系结构,研究了MS.NET Remoting技术在异地协同的复杂产品智能设计系统中的应用,并以坦克动力舱智能布置系统为例,利用.NET Remoting技术构建分布式协同设计平台,较好的解决了协同设计过程中的不同应用程序之间数据实时交互、复杂方法调用等问题.