共查询到20条相似文献,搜索用时 46 毫秒
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固相合成法是胸腺五肽生产的主流技术。本文采用对碱敏感的Fmoc保护Na-氨基。用二甲基甲酰胺为溶剂合成胸腺五肽。本文阐述了其基本原理、采用此法的原因、合成工艺及纯化方法。 相似文献
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目的:输血是恶性肿瘤临床治疗的常用策略,而含有白细胞的血液制品会抑制免疫功能.胸腺素是一种免疫调节多效细胞因子,用于癌症或感染性疾病患者,提示其对癌症手术切除患者输血疗效的影响.本研究旨在探讨胸腺素在恶性肿瘤患者输血中的免疫调节作用.方法:本研究纳入80例结直肠癌患者(男47例,女33例).将患者随机分为输注无白细胞混... 相似文献
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胸腺五肽固相合成及免疫增强作用的研究 总被引:12,自引:0,他引:12
目的 合成有生物活性的胸腺五肽并研究其免疫增强作用。方法 用Fmoc固相肽合成法合成胸腺五肽 ,用E玫瑰花实验检测生物活性。合成的胸腺五肽经小鼠腹腔注射后进行淋巴细胞转化实验、溶血空斑实验及IL 2活性实验 ,研究其免疫增强作用。结果 合成 2批胸腺五肽 ,精肽产量分别为 2 7mg和 3 4mg ,具有生物活性 ,实验组动物脾淋巴细胞的转化率、空斑形成细胞及IL 2活性皆明显高于空白对照组。结论 用Fmoc固相肽合成法可以成功地合成有生物活性的胸腺五肽 ,且具有免疫增强作用。 相似文献
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在研究现有正丙基膦酸酐合成工艺的基础上,以溴代正丙烷与亚磷酸二乙酯为起始原料经烷基化酸解与脱水缩合三步反应得到高含量正丙基膦酸酐。将其作为脱水剂应用于胸腺五肽(TP5)的合成,与常规二代缩合剂HBTU(苯并三氮唑-N,N,N’,N’-四甲基脲六氟磷酸盐)相比,具有脱水效率高,后处理方便等优点。 相似文献
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《分离科学与技术》2012,47(13):3393-3416
Abstract A numerical method is presented for determining flow rates in centrifugal isotope separation cascades that satisfy the required “ideal cascade” conditions. This method is an iterative method, which in the iteration process uses the q-iteration method to give the concentration and distributions of components under a specified hydraulic status for a cascade; and then, based on the concentration distributions, solves the algebraic equations describing the hydraulic status of the cascade to improve the specified hydraulic status towards the required “ideal cascade” conditions. The material losses in the cascade pipes and centrifuges are taken into account. Numerical simulations are performed by taking an MARC cascade with variant stage separation factors as an example. The results from four cases with and without material losses are compared. The results demonstrate that the method works very well, and show that when the material losses are nonexistent or very small, the concentration distributions can exactly satisfy the “ideal cascade” condition; but when the material losses are large, the “ideal cascade” condition can not be satisfied, but the method can produce a solution that allows the condition to be approximately satisfied. 相似文献
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目的建立一种高效、简便、实用的分离纯化葡萄球菌蛋白A(SPA)的工艺。方法采用超声波破菌和IgG-Sepharose4B亲和层析分离纯化SPA;以Lowry法检测蛋白含量;双向琼脂免疫扩散法测定效价和特异性;用还原和非还原SDS-PAGE法检测纯度和相对分子质量。结果此法制得的3批SPA的蛋白含量分别为4·7、4·4和5·4mg/ml,收率分别为2·70、2·64和3·78g/100g湿菌。对人IgG的免疫双扩散效价均为1:64;与正常人、豚鼠、家兔和小鼠的血清反应均出现一条沉淀线,而与鸡和羊不出现沉淀线。经非还原SDS-PAGE检测只呈现一条蛋白带,相对分子质量约为160000~180000;经还原SDS-PAGE检测呈现两条蛋白带,相对分子质量分别约为67000和34000。结论已成功建立了分离纯化SPA的新工艺。 相似文献
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《分离科学与技术》2012,47(4):695-709
Abstract It is shown theoretically that a novel process of hydrogen isotope separation can be obtained by combining bithermal isotopic exchange with transport through a selective permeable membrane. The theory of the process predicts that a small column with high separation and very small inventory can be realized. The method is attractive especially for small and medium scale processes, such as tritium enrichment, heavy water upgrading, and final deuterium concentration. 相似文献
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Allylsucrose has been prepared by reaction of sucrose with allyl chloride in the NaOH-DMSO system. DS^↑- (average degree of substitution) of allylsucrose calculated by elemental analysis was 6.7, and the efficiency of allyl chloride was 83.7%. The mechanism of the reaction was based on the acceleration of base-catalyzed reactions in dipolar aprotic solvents. The novel method is facile and suitable for large-scale preparation. 相似文献
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A new operation policy-quasi-batch distillation for recovering infinitesimal amounts species existing in a mixture is presented. In quasi-batch distillation operation, feed is introduced with a constant flow rate onto the feed stage of the column, and the flow rate of the distillate is the same as that of the feed, whereas the bottom product is withdrawn periodically. The behavior of quasi-batch distillation is simulated and analyzed through an example on heavy water separation. Comparing with continuous or batch distillation, the new operation policy is more reliable and efficient to achieve higher recovery of dilute component. This is especially suitable for separating small amount of precious species from large amount of raw material. 相似文献
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5-氨基乙酰丙酸(ALA)在农业、医学等领域应用广泛,市场前景广阔。今采用膜分离、反应萃取和浓缩结晶相结合的新工艺从发酵液中提取5-氨基乙酰丙酸盐酸盐(ALA·HCl),有效提高了产品的回收率,降低了生产成本。分离过程中先对离心后的发酵液进行超滤除杂,反渗透浓缩,预处理过程ALA回收率为86.4%;然后以体积比1:1的磷酸二(2-乙基己基)酯-正己醇混合液为萃取剂,20℃下进行二级萃取。初始水相pH6.5,两次萃取有机相和水相的体积比R均为0.5;两级萃取所得的有机相混合后,再在30℃下用1mol·L-1盐酸对有机相进行反萃取,水相和有机相的体积比r为0.5。萃取过程ALA的回收率为79.8%。所得反萃水相经脱色、真空蒸发浓缩后二次冷却结晶。分离过程ALA·HCl的总收率达到55.2%,产品纯度大于99.5%。 相似文献
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Hui Wang Tong Wang Anthony L. PomettoIII Lawrence A. Johnson 《Journal of the American Oil Chemists' Society》2009,86(12):1241-1250
In the dry-grind corn ethanol industry, horizontal decanter centrifuges are used to separate the whole stillage into wet grains
and thin stillage. The wet grains mixed with condensed thin stillage are dried to form dried distiller’s grains with solubles
(DDGS). In order to investigate the effect of different corn breaking treatments on increasing oil partitioning in thin stillage,
a laboratory method is needed to simulate industrial decanting where a typical thin stillage is produced. The thin stillage
obtained using a conventional laboratory centrifuge had much lower solids content and less than one-half of the dry-matter
yield compared to the industry counterpart because the conventional laboratory centrifuge and industry decanter centrifuge
have different separation mechanisms. By evaluating the properties of industrial thin stillage and the mechanism of industrial
decanter centrifugation, a laboratory decanting device was designed and a decanting procedure, the multiple-wash centrifugal
filtration (MWCF) method, was developed. This method involves multiple steps of filtration under centrifugal force after washing
the solids with the liquid generated from the same mash. Four cycles of MWCF produced a thin stillage with similar solids
content (7.3 vs. 7.2%), dry-matter yield (54.2 vs. 54.7%), and wet yield (83.3 vs. 80.6%) compared to industrial thin stillage.
The presence of ethanol did not influence the laboratory decanting results, which indicates the application robustness of
this laboratory thin stillage preparation method. 相似文献
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《分离科学与技术》2012,47(11-12):3269-3285
Abstract Five different samples of mesoporous zirconium silicate have been prepared with various mole ratios of Si/Zr and Cetyltrimethylammonium bromide (CTMABr) as template. XRD, nitrogen sorption, SEM, IR, thermogravimetry and sorption of radionuclides have been studied. Separation of Cs(I)-Mo(VI), Sr(II)-Mo(VI), Cs(I)-Th(IV), Sr(II)-Th(IV), and Tl(I)-Th(IV) have been developed on columns of this novel sorbent. 相似文献