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基于微生物发酵过程中发酵液电容率β-分布的特性,研究了一种采用电容式传感器在线检测发酵液活菌浓度的检测系统。 相似文献
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基于微生物发酵过程中发酵液电容率β-分布的特性,研究了一种采用电容式传感器在线检测发酵液活菌浓度的检测系统. 相似文献
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基于干法光学生物传感器的便携式血红蛋白检测系统 总被引:1,自引:0,他引:1
主要介绍了一种基于干法光学生物传感器的便携式血红蛋白(Hb)检测系统.该系统基于光学反射原理,以高精度微转换器ADμC824为控制核心、高亮度发光二极管(LED)为光源、光敏电阻为光电转换器件,配合高精度采集/放大电路,实现Hb信号的采集和处理.与生化仪器Reflotron Plus的对比性测试实验表明,该系统线性测试范围为13.7~18.7 g/dL,相关系数为0.981.该系统设计体积(18.5×13×6)cm3、检测时间60 s、可存储255条记录,并具备测量记录显示、查询和删除等功能.同时,该系统还可与外部计算机通讯,实现测试过程实时监测和所有测试记录一次性上传等.该系统将在Hb现场快速检测和血液筛查中有广泛的应用前景. 相似文献
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生物免疫传感器检测迟缓爱德华氏菌研究 总被引:1,自引:0,他引:1
迟缓爱德华氏菌(E.tarda)是最为严重的水产动物致病菌之一,准确、即时的检测手段是预防控制该菌传播的关键所在.通过量子点(QDs)标记E.tarda单克隆抗体(Ab),利用生物免疫传感器技术实现E.tarda的快速、特异性检测.结果显示,QDs-Ab的荧光通过加入氧化石墨烯(GO)产生淬灭,构建了捕获目标细菌的探针,GO最适淬灭浓度为60μg/L.细菌捕获探针中加入E.tarda后,能够检测到重新恢复强度的橙色荧光.针对E.tarda设计的生物免疫传感器的特异性,选取灿烂弧菌、溶藻胶弧菌、副溶血弧菌和嗜水气单胞菌作为对照,结果显示,对照组不能明显引起荧光强度的改变,而实验组却能显著提高荧光强度.本研究建立的基于荧光能量共振转移(FRET)的具有高灵敏度和特异性的生物传感器检测方法在细菌的早期诊断中有良好的应用潜质. 相似文献
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电化学/生物传感器快速检测大肠杆菌的研究进展 总被引:2,自引:0,他引:2
大肠杆菌广泛分布于自然界中,通常被用来作为水体系统排泄物污染情况的指示菌.它是大面积食物中毒的主要原因之一,严重感染者会引发败血症、肾功能衰竭等危及生命的并发症.电化学/生物传感器具有独特的优势,如能在浑浊溶液中操作、选择性好、灵敏度高、检测速度快等,因此在临床检测、环境保护和食品安全等领域得到了广泛应用.该文主要对电化学,生物传感器快速检测大肠杆菌的研究进展进行了简要的综述. 相似文献
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介绍了一种采用不规则四边形棱镜设计的小型化表面等离子体共振(SPR)生物传感器。棱镜结构与已有的TI Spreeta传感器类似,但是在尺寸、光学性能等方面做了较大优化。新研制的SPR传感器在光学检测精度和系统集成性等方面也有了很大提高。在光路设计中,采用波长为630 nm的宽光束红光LED作为光源,5 000像素点线阵CCD作为光电检测器,光学检测效果要大大优于TI Spreeta波长为830 nm的近红外光源和128像素点的线阵硅光二极管。在光路优化的同时,系统集成了流动控制模块、信号采集处理模块,形成了一个可实现生物大分子相互作用分析的集成小型化SPR检测装置。利用空气、水及乙醇等进行的SPR实验表明:该装置能够对单一样本进行精确检测,共振角的检测精度高达0.01°,且检测结果线性度高,稳定性好,单一样本的检测偏差小于0.5%。 相似文献
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Detection of sulphamethazine with an optical biosensor and anti-idiotypic antibodies 总被引:3,自引:0,他引:3
We describe the development of an assay for the detection of sulphamethazine in animal urine with a surface plasmon resonance (SPR) biosensor. In order to obtain a general assay that can easily be transferred to other veterinary drug residues, a monoclonal antibody against sulphamethazine and a corresponding anti-idiotypic antibody were used. The assay had a lower detection limit of 5 μg/l which is well below the maximum residue limits (MRL) of 100 μg/l and can be used for screening purposes in animal urine. The binding reaction between the antibodies as occurring during sensor application was characterized with respect to avidity and kinetic properties. 相似文献
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应用亲和型生物传感器IAsys实时监测白细胞介素-2(IL-2)与其可溶性受体(sIL-2R)之间的相互作用,并进一步揭示IL-2/sIL-2R在体内的作用机理。将sIL-2R进行生物素标记后,利用生物素-亲和素系统将其固定于IAsys生物传感器的生物素样品池表面,向样品池中加入IL-2,通过检测由IL-2与固定于样品池表面的sIL-2R结合而引起的传感基片表面共振光角度的变化来研究IL-2和sIL-2R之间的相互作用。IL-2可与sIL-2R在体外发生特异性结合。IAsys生物传感器是一种研究生物大分子间相互作用的理想工具,为细胞因子/受体体系的相互作用研究提供了有效的方法和新的思路。 相似文献
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结合三磷酸腺苷(ATP)生物发光原理和免疫磁分离技术,研制了一种细菌特异性快速检测系统,可实现对细菌的特异性高灵敏度快速检测.该仪器通过改进光学检测模块的避光设计和放大采集电路的噪声抑制设计,及严格控制试剂反应条件,降低了检测背景,从而达到了更低的检测下限并提高了检测灵敏度.对大肠肝菌(ATCC25922)标准菌溶液进行检测,检测下限达到18 CFU/mL, 与培养计数法检测结果相关系数R达到0.993,选取一份样品进行重复性检测,相对平均偏差(R.A.D.)为6.48 %,变异系数(CV)为9.33 %.结合免疫磁分离技术,对混入的高浓度金黄色葡萄球菌的大肠杆菌溶液进行检测,检测结果与原始大肠肝菌培养计数结果一致,证明该仪器及方法对检测大肠肝菌具有良好的特异性.该系统检测速度快,准确度较高,重复性良好,特异性好,可用于食品及环境中的致病菌快速检测. 相似文献
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Gold (Au) and platinum (Pt) were used as the working electrode material to detect cholesterol in solution through enzymatically generated hydrogen peroxide (H2O2). Both gold and platinum were capable of detecting cholesterol through the electrochemical oxidation of H2O2, and could be used as the working electrode material. By comparison, however, Au was preferable over Pt in terms of higher response current and better sensitivity. Therefore, Au was chosen as the working electrode material for the fabrication of a thick-film screen-printed cholesterol biosensor consisting of three electrodes on an alumina substrate (working: Au, reference: Ag/AgCl, and counter: Au). The immobilization of the enzyme cholesterol oxidase (ChOx, E.C. 1.1.3.6) on the Au working electrode was achieved using a self-assembly approach. A thiol, 3-mercaptopropionic acid (MPA), was self-assembled onto the gold working electrode forming a thin organic layer that served as the anchor for the enzyme immobilization. 1-Ethyl-3(3-dimethylamino propyl)carbodiimide methiodide (EDC) was then used to immobilize the enzyme ChOx covalently on the gold working electrode through the carbodiimide coupling between the carboxyl (–COOH) groups of the self-assembled MPA layer and the amino (–NH2) groups of the enzyme. Electrochemical measurements showed that this biosensor responded well to cholesterol, confirming that the self-assembly immobilization method was effective. The reproducibility, the interference, and the storage stability of the biosensor were studied and assessed. 相似文献
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Qingxiang WangAuthor Vitae Bin ZhangAuthor VitaeXiaoqiang LinAuthor Vitae Wen WengAuthor Vitae 《Sensors and actuators. B, Chemical》2011,156(2):599-605
A label-free DNA biosensor for hybridization detection of short DNA species related to the transgenic plants gene fragment of cauliflower mosaic virus (CaMV) 35S promoter was developed in this paper. The nanocomposite containing chitosan (CS) and mutiwalled carbon nanotubes (MWNTs) was first coated on a glassy carbon electrode. Then a highly reactive dialdehyde reagent of glutaraldehyde (GTD) was applied as an arm linker to covalently graft the 5′-amino modified probe DNA to the CS-MWNTs surface via the facile aldehyde-ammonia condensation reaction. The hybridization capacity of the developed biosensor was monitored with electrochemical impedance spectroscopy (EIS) using [Fe(CN)6]3−/4− as an indicating probe, and the experimental results showed that the biosensor had fast hybridization rate and low background interference. A wide dynamic detection range (1.0 × 10−13-5 × 10−10 M) and a low detection limit (8.5 × 10−14 M) were achieved for the complementary sequence. In addition, the hybridization specificity experiments showed that the sensing system can accurately discriminate complementary sequence from mismatch and noncomplementary sequences. 相似文献
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随着人们对安全监控的需求,视频监控中的异常事件检测越来越引起人们的关注。介绍了一种聚众异常事件检测的新方法,其核心是感兴趣点光流信息的变化。当使用背景差分法所获得的前景大于设定阈值,并且目标之间相互距离在某一范围内时,提取感兴趣点,使用光流法跟踪感兴趣点,然后计算熵值,一旦熵值大于设定的阈值,则聚众事件发生,系统报警;否则,将该帧熵值设置为某一固定值,继续下一帧的背景差分处理。通过实拍视频序列验证了该算法的有效性。 相似文献
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Jin CaiAuthor Vitae Chunyan YaoAuthor Vitae Ji XiaAuthor VitaeJue WangAuthor Vitae Ming ChenAuthor VitaeJunfu HuangAuthor Vitae Kai ChangAuthor VitaeChunjiang LiuAuthor Vitae Hong PanAuthor VitaeWeiling FuAuthor Vitae 《Sensors and actuators. B, Chemical》2011,155(2):500-504
We developed a 2 × 5 model quartz crystal microbalance (QCM) DNA biosensor array for detection of five bacteria, which based on hybridization analysis of bacterial 16S-23S rDNA internal transcribed spacer (ITS) region. A pair of universal primers was designed for PCR amplification of the ITSs. The PCR products were analyzed by the biosensor. We used gold nanoparticles to amplify the frequency shift signals. Fifty clinical samples were detected by both the biosensor and conventional bacteria culture method. We found a linear quantitative relationship between frequency shift and logarithmic concentration of synthesized oligonucleotides or bacteria cells. The measurable concentration ranged from 10−12 to 10−8 M for synthesized oligonucleotides and 1.5 × 102 to 1.5 × 108 CFU/mL for bacteria. The 10−12 M of synthesized oligonucleotides or 1.5 × 102 CFU/mL of Pseudomonas aeruginosa could be detected by the biosensor system. The detection could be completed within 5 h including the PCR amplification procedure. Compared with bacteria culture method, the detection sensitivity and specificity of the biosensor system were 94.12% and 90.91%, respectively. There was no significant difference between these two methods (P = 0.625 > 0.05). The biosensor system provides a rapid and sensitive method for parallelized and quantitative analysis of multiple pathogenic bacteria in clinical diagnosis. 相似文献