THP-1 源巨噬细胞上清液对类风湿性关节炎 成纤维细胞和血管内皮细胞的作用

在类风湿关节炎(Rheumatoid Arthritis,RA)病理过程中,成纤维细胞的“肿瘤”样转型被认 为与 M1 型巨噬细胞分泌的诱导因子有关,表现为增殖过度、凋亡减少以及异常分泌促炎症因子。此 外,成纤维细胞的异常转型也会通过反馈调节进一步刺激巨噬细胞向 M1 型极化。目前关于 M1 型巨 噬细胞对成纤维细胞和血管内皮细胞的体外作用尚未有报道。为探讨 RA 滑膜中不同细胞间的相互作 用,该研究利用实时荧光定量法检测人髓系白血病单核细胞(THP-1)源 M0、M1 型巨噬细胞中白细 胞介素-8(IL-8)和内皮型一氧化氮合酶(eNOS)基因的 mRNA 表达量,酶联免疫吸附法测定 THP-1 源 M1 型巨噬细胞上清液炎症因子的含量,通过细胞增殖实验测定不同比例 M1 型巨噬细胞上清液对成 纤维细胞和血管内皮细胞的增殖影响。结果显示,THP-1 源 M1 型巨噬细胞主要分泌白细胞介素-1β、 白细胞介素-8 蛋白。对类风湿关节炎滑膜成纤维细胞(MH7A)而言, M1 型巨噬细胞上清液与含血 清 RPMI(Roswell Park Memorial Institute)培养基混合(比例为 7∶3)作用 48 h 能促进细胞增殖;对人脐 静脉细胞融合细胞(EA.hy926)而言,M1 型巨噬细胞上清液与含血清 DMEM(Dulbecco Modified Eagle Medium)培养基混合(比例为 6∶4 或 7∶3)处理 72 h 显著促进了细胞的增殖。该研究可为建立 RA 血管 翳类组织模型提供前期研究的数据支撑,有助于体外构建抗 RA 药物高通量筛选平台。     

4-X-N-Y-6-氮杂雄-17-羰基-Z-4-烯-3-酮衍生物对3BHSD的抑制活性与结构关系的研究

应用分子力学方法MM,和半经验量子化学AM1法,获得22种4-X-N-Y-6-氮杂雄-17-羰基-Z-4-烯-3-酮衍生物的优势构象,再利用量子化学算法和分子图形学技术,获得电子结构参数和几何结构参数,采用多元线性回归分析和人工神经网络误差反传算法(BP),将这些参数和衍生物对3β-羟类固醇脱氢酶(3BHSD)的抑制活性相关联。结果表明,衍生物对3BHSD的抑制活性大小和分子最高占用轨道能(E_(HOMO))、16号碳原子的净电荷(Q)及5号碳原子和6号氮原子间键长的相关性较好,成功地建立了22种衍生物的构效关系式。HOMO轨道组成分析表明,A环上的4号、5号碳原子和羰基氧原子(O_(1(?)))及B环上的6号氮原子和7号碳原子可能是与受体作用时的活性位点。     

凹凸科技进军网络安全领域

2006年2月28日,网络安全及电源管理芯片供应商凹凸科技(O2Micro)向中国用户隆重推介凹凸科技在网络安全领域的独特理念和全新方案.此举标志着凹凸科技已正式进军网络安全领域.特别在ASIC防火墙和SSL VPN技术和产品的竞争中,凹凸科技已经担负了不可或缺的重要角色.而其在模拟与数字集成电路方面拥有的强大设计和制造能力,无疑将为其最终成为全球网络安全领域的主要供应商增加关键筹码.凹凸科技在评述中国安全市场发展趋势的同时,还将携两款最具代表性的成熟产品--ASIC防火墙SifoWorks和SSL VPN产品Succendo一起亮相.     

2006 Sun科技日在中国举行——"Java之父"James Gosling再来中国

继成功举办2005年Sun JAVA中国开发者大会之后,Sun一年一度全球规模的开发者大会——2006Sun科技日又分别于今年9月23～24日和27～28日相继在中国上海和北京两大都市举行“。Java之父”James Gosling再次来到中国,与中国的开发者讨论软件开发者所关心的问题,讲述Sun的软件发展策略、介绍Solaris10操作系统的独特的特性和新的Java开发工具、展望Java技术发展前景,特别是阐明Sun实施Java技术开源的策略。Sun科技日是Sun公司最重要的全球开发者的大会,它针对Java技术、Solaris10OS、NetBeans、OpenSolaris、开源技术、开发工具和Web…     

陕西省第一届国防科工系统内网安全研讨会召开

2006年3月15日,“陕西省第一届国防科工系统内网信息安全建设专题研讨会”在西安市隆重召开。此次会议的议题是,在当前的信息安全形势下,陕西省国防科工系统内网面临的信息安全问题,以及如何采取有效的解决方案进一步提高国家机要部门内部网络的安全保护等级,以适应形势发展的需要。此次会议由陕西省国防科工委主办,陕西省国防科工委信息中心和北京榕基网安科技有限公司、北京理工先河科技发展有限公司、陕西金叶西工大共同承办,国家保密局和陕西省国防科工委的有关领导出席会议并发表了重要讲话。在为期两天的会议中,共有100多名国防科工系…     

CP Secure正式进军中国市场

2006年8月8日CPSecure(China)公司在北京成立,这是CPSecure公司第一次在中国市场正式登台亮相。作为一个在业内具有领先技术的专业安全厂商,CPSecure拥有全球最顶尖的信息安全专家,一直致力于内容安全网关产品的研发、生产、销售、服务和咨询。此次登陆中国市场的CPSecure公司将通过自己强大的研发、销售和技术支持队伍,为中国用户提供最适和的产品和最快捷的技术支持。顶尖的技术造就领先的CPSecureCPSecure成立于2002年,由前趋势执行副总裁DavidLu(吕鸿武)和研发总监ShuangJi(季爽)共同创立。公司总部位于美国加州,在欧洲、中国…     

在网络层实现安全传输通道技术的研究

安全传输通道是对传输的原始信息进行加密和协议封装处理,从而实现安全传输的技术,本文在TCP/IP的网络层上提出了实现安全传输通道的技术和解决方案。并对网络层的安全传输通道技术提出了实现机制。     

Teacher education in IT in Germany

Abstract In 1988 the eleven West-German Kultusminister , with responsibility for the school systems in their respective Bundeslaender , reached agreement with the Federal Ministry of Education and Science on the broad concept of 'Information and Communications Technology', ITG. After four years of discussion it was agreed that ITG should be introduced into the secondary level of the general schools. In spite of strong disagreements about the ways and speed of implementation, and after several years of experiments, important parts of the concept have become a reality in practically all of the old Bundeslaender. While acknowledging the complexity of the German educational system the paper attempts to outline the development of the concept of Information and Communications Technology and its consequences in teacher education. The development in the eastern part of Germany, the former GDR, has been completely different to that in the western part and this is also commented upon in the paper.     

IT in teacher education in Denmark

Abstract As with many European countries Denmark is experiencing change in teacher education and most notably in the wake of an act of government in 1992 which was designed to set out common aims for teacher education. Prior to that the content and structure of teacher education had been highly specified including, for example, the requirement that student teachers should take a course of 42 hours IT-related lessons. This paper provides a brief history of the background to the changes in teacher education and focuses on the implications that the more flexible aims for teacher education have for the future integration of IT in teacher education.     

Informatics in teacher education in Japan

Abstract This paper charts the developments in information technology (IT) policies in education in Japan over the past decade with particular emphasis on the impacts on teacher education. Beginning with the work of the National Council on Educational Reform in 1985, the Japanese education system initiated a thorough analysis of the role of information technology in all aspects of education from kindergarten through to high school, vocational education and pre- and in-service teacher education. The various approaches culminated in guidelines and statutory requirements relating to both schools' curricula and teacher training, published in the period 1990–1992. More recently other ministries have contributed to developments including the Ministry for International Trade and Industry which has categorized the different types of information engineers and among these is the new Educational Engineer. The paper reports the processes leading to these developments.     

IT in teacher education in Scotland

Abstract This paper outlines the consensual approach to the formulation of curriculum policy in general, and in relation to information technology (IT) in particular, in Scotland. It outlines the approaches to IT that have emerged as a result. These are founded on the premises that all children should develop knowledge and understanding about IT and skills in using IT; that IT should influence both content and delivery of the curriculum; and that IT (computing studies in Scotland) should be an elective for secondary school pupils. Against this background, the paper describes the evolution of policy and practice relating to IT in teacher education. It appraises the strategies which institutes are using for developing the professional skills necessary to deliver IT in the Scottish curriculum. It notes significant progress but highlights the need to develop strategies which integrate IT more deeply with other professional studies so that student teachers develop deeper, more reflective approaches to IT.