油酸诱导幼猪急性肺损伤模型的建立

目的 建立幼猪油酸所致肺损伤模型,并观察最佳油酸成模剂量.方法 取5～7周龄、体质量5～7 kg的健康雄性幼猪15只,随机分为实验组(n=9只)及对照组(n=6只).各组动物均予经口气管插管后呼吸机机械通气,颈内动脉及颈外静脉置管.实验组幼猪予油酸80 ～150mg·kg-1用9 g·L-1盐水稀释至15 mL,微量泵经其颈外静脉持续60 min缓慢注入右心室,对照组幼猪予9 g·L-1盐水15 mL微泵维持60 min静脉输入,取不同时间点(实验组0h～成模时;对照组0～6h)监测生命体征及各项呼吸力学、血气参数、血流动力学参数,至血氧分压/吸入氧体积分数[pa(O2)/FiO2] ＜39.4 kPa确认成模,实验组中3只动物立即处死,其余动物撤机后维持存活1周,实验结束后取左侧肺组织行病理学检测.结果 油酸泵入3～14h,实验组pa(O2)/FiO2值出现显著下降,与对照组比较差异有统计学意义(P＜0.05),肺形态学达急性肺损伤标准.其中实验组7只幼猪至3～8hpa(O2)/FiO2值均明显下降,油酸剂量为80～120 mg · kg-1,另2只成模时间分别为11 h及14 h,使用油酸剂量达150 mg·kg-1.对照组至通气6hpa( O2)/FiO2值在各时间点差异均无统计学意义.实验组动物在通气起始,体循环动脉血压(SBP)及心率较为稳定,随着通气时间延长,实验组及对照组SBP、心率变化无统计学意义.结论 使用油酸80～150 mg·kg-1可成功建立幼猪油酸所致肺损伤模型,最佳油酸成模剂量为80～120 mg·kg-1.     

内源性急性肺损伤大鼠模型的建立

目的 建立大鼠肺内源性急性肺损伤/急性呼吸窘迫综合征(ALI/ARDS)模型.方法 大肠杆菌[O111B4,(4.0～6.0)×1012 CFU/L]3 ml/kg气管注射制作大鼠肺内源性ALI模型.分别于注射大肠杆菌后12、24、36、48、72 h共5个时间点给予机械通气,监测氧合指数(PaO_2/FiO_2)及胸肺动态顺应性(Cdyn).肺组织常规固定进行病理学检查.结果 12、24、36、48、72 h大鼠PaO_2/FiO_2分别为(30.71±7.95)kPa、(21.66±5.34)kPa、(21.09±4.75)kPa、(25.01±8.78)kPa、(33.82±8.02)kPa.均低于正常组(63.82±3.03)kPa(P＜0.01).12、24、36、48、72 h大鼠Cdyn分别为(4.23±0.13)ml/(kg·kPa)、(4.19±0.96)ml/(kg·kPa)、(4.28±0.69)ml/(kg·kPa)、(4.44±0.62)ml/(kg·kPa)、(4.58±0.35)ml/(kg·kPa),均低于正常组(8.16±0.78)ml/(kg·kPa)(P＜0.01).12、24、36、48、72 h大鼠ALI发生率分别为71.4%、100%、100%、83.3%、57.1%;ARDS发生率分别为28.6%、85.7%、83.3%、66.7%、14.3%.病理学改变方面,随着感染时间延长,由肺内少量渗出液到肺泡壁明显水肿.肺泡腔内出现大量渗出液和炎症细胞,可见透明膜形成以及严重肺泡萎陷,但在72 h炎症已明显减轻.结论 本实验经气管滴入大肠杆菌建立了肺内源性ALI/ARDS动物模型,分时间点观察了病情演变,为有效利用动物模型提供了资料.     

部分液体通气对急性肺损伤幼猪心肺功能的影响

目的 研究部分液体通气对急性肺损伤幼猪心肺功能的影响.方法 12只上海小白猪,随机分为对照组(n=6)和治疗组(n=6).内毒素60 μg/kg静脉维持诱导肺损伤.治疗组成模后予高氟化碳(C10F18)10 ml/kg灌入气管.于基础状态,成模时,成模后1、2、4 h分别行血气分析、记录心率、血压、呼吸、肺动态顺应性、气道阻力、脉搏轮廓心输出量、全身血管阻力.结果 两组动物成模时氧合指数、肺动态顺应性、脉搏轮廓心输出量均明显下降,各参数组间比较无统计学差异.2 h后对照组氧合指数193.8±47.5,肺动态顺应性(0.8±1.7) ml/(cm H2O·kg),4 h后心输出量(2.1±0.7) L/min;治疗组2 h后氧合指数为261.5±25.8,肺动态顺应性(1.2±0.7) ml/(cm H2O·kg),4 h后心输出量(3.1±0.2) L/min,较对照组均有改善,差异有显著性(P＜0.05).结论 部分液体通气可明显改善肺损伤动物心肺功能.     

肺复张策略救治急性肺损伤幼猪的实验研究

目的：通过急性肺损伤（ALI）幼猪模型,研究肺复张策略（RM）的可行性和有效性,并探讨其对损伤后肺组织修复的影响。方法：健康幼猪12只,注射内毒素（LPS）成模后,随机分为常规潮气量通气组（CON组）和小潮气量联合RM通气组（RM组）,观察8 h,动态监测血液动力学、肺动态顺应性及血气分析指标,测定血浆、肺泡灌洗液中转化生长因子（TGF-β1）浓度,实时荧光定量PCR法检测TGF-β1在肺组织中的mRNA表达水平,并观察肺组织的病理学改变。结果：两组幼猪的心输出量和外周血管阻力指标差异无统计学意义;而RM组的血管外肺水指数在成模6 h以后,肺血管通透性指数于成模8 h时均较CON组明显下降（P＜0.05）;RM组的肺动态顺应性明显高于同时间点的CON组（P＜0.05）;RM组PaO2/FiO2值于成模2 h后明显高于CON组,而且RM组的肺泡-动脉氧分压差在成模2 h后即明显下降（P＜0.05）;RM组血浆、肺泡灌洗液中TGF-β1浓度及其在肺组织中的mRNA表达水平均低于相应CON组;RM组的肺泡扩张度明显高于CON组,而肺损伤评分明显下降（P＜0.05）。结论：RM可以改善ALI幼猪的气体交换和肺动态顺应性,且安全有效,能够扩张肺泡并减轻肺损伤程度,有助于改善损伤后的肺修复过程。     

连续性血液透析滤过对内毒素诱导急性肺损伤幼猪血流动力学影响

目的 探讨连续性血液透析滤过对稳定内毒素诱导急性肺损伤幼猪血流动力学的价值.方法 18头幼猪随机分为对照组、肝素组和干预组,每组6头.内毒素诱导肺损伤.于动物基础状态(B)、成模(A 0 h)、成模后2 h(A 2 h)、4 h(A 4 h)、6 h(A 6 h)记录心率(HR)、平均动脉压(MABP)、脉搏轮廓心输出量指数(PCCI)、全身血管阻力指数(SVRI)、心功能指数(CFI)、左心室收缩力指数(dPmx)、肺血管外肺水指数(EVLWI).结果 A 0 h各组PCCI、MABP、CFI、dPmx较B下降;HR、SVRI、EVLWI较B上升,组间比较无统计学意义.A 4 h各组EVLWI、SVRI、CFI、dPmx:干预组(15.0±1.9)ml/kg、(3073.0±685.0)dyns·cm-5·m2、(5.3±0.5)L/min、[(1883.0±466.0)mmHg/s,1 mm Hg=0.133 kPa];对照组(34.3±5.7)ml/kg、(4991.0±574.0)dyns·cm-5·m2、(3.6±0.4)L/min、(713.0±211.0)mm Hg/s;肝素组(34.3±5.1)ml/kg、(5445.0±576.0)dyns·cm-5m2、(3.3±0.2)L/min、(768.0±247.0)mm Hg/s.A 6 h各组HR、MABP、PCCI:干预组(154.2±12.4)/min、(97.2±10.3)mm Hg、(3.9±0.5)L/(min·m2);对照组(172.0±2.8)/min、(76.2±10.8)mm Hg、(2.7±0.5)L/(min·m2);肝素组(174.5±7.6)/min、(76.0±10.2)mm Hg、(2.8±0.4)L/(min·m2).干预组与对照组和肝素组比较差异有统计学意义(P<0.05),肝素组与对照组比较差异无统计学意义.结论 连续性血液透析滤过对稳定内毒素诱导急性肺损伤幼猪血流动力学状态有效.     

连续性血液透析滤过治疗对急性肺损伤幼猪凝血功能的影响

目的 对内毒素诱导急性肺损伤(ALI)幼猪行连续性血液透析滤过治疗,观察其对凝血功能的影响.方法 18头小白猪随机分为对照组、肝素组和治疗组,每组各6头.予内毒素150μg/kg静脉注射诱导肺损伤.成模后治疗组行连续性血液透析滤过6 h,血流速度50 ml/min,透析液速度600 ml/h,置换液速度300 ml/h,肝素持续泵入保持活化凝血时间(ACT)180～250 s.成模后肝素组予肝素静脉维持保持ACT 180～250 s.于动物基础状态(B)、成模(A0h)、成模后2 h(A2h)、4 h(A4h)、6 h(A6h)分别采静脉血行外周血细胞计数、凝血酶原时间(PT)、白陶土部分凝血活酶时间(APTT)、纤维蛋白原(FiB)、D-二聚体(D-D),采动脉血行血气分析.记录心率(HR)、呼吸(RR)、血压(MABP)、肺动态顺应性(Cdyn).结果 各组动物A0h改良氧合指数(P/F)、Cdyn、MABP较B下降,HR、RR较B上升,PT和APTT延长,FiB下降,D-D增加,组间比较差异无统计学意义;A6h治疗组动物P/F、Cdyn、HR、MABP与对照组和肝素组比较差异有统计学意义.实验中PT、APTT进行性延长,组间比较PT差异无统计学意义,治疗组A2h、A4h、A6h APTT与对照组比较差异有统计学意义,肝素组A6h APTT与对照组比较差异有统计学意义.A6h治疗组和肝素组纤维蛋白原较对照组有明显改善.治疗组A2h、A4h、A6h D-D均较对照组改善,肝素组A4 h、A6h D-D较对照组改善,差异有统计学意义(P＜0.05).结论 连续性血液透析滤过可改善肺损伤动物的氧合、呼吸力学,对动物的凝血状态无不利影响.     

急性肺损伤幼猪肠黏膜上皮细胞凋亡的表达

目的 观察细胞凋亡在急性肺损伤(AU)幼猪肠黏膜上皮的发生情况及相关机制.方法 采用脂多糖(LPS)诱导幼猪ALI模型,分离肠黏膜上皮细胞,采用流式细胞结合末端脱氧核苷酸转移酶介导的dUTP原位缺口末端标记(TUNEL)和电镜方法观察凋亡细胞的改变.同时检测炎症/抗炎因子TNF-α/IL-10 mRNA的表达和氧化与抗氧化损伤指标丙二醛(MDA)、超氧化物歧化酶(SOD)水平与凋亡发生的关系,进一步阐明凋亡的发生机制.结果 模型组肠上皮细胞凋亡较正常组明显增加(P<0.01);模型组TNF-α mRNA表达增强,IL-10mRNA表达减弱,MDA含量增加、SOD活性减低与正常组比较差异均有统计学意义(P<0.01).结论 ALI幼猪肠黏膜上皮细胞凋亡增加,与炎症/抗炎因子表达失衡,氧化/抗氧化损伤异常有关.     

吸入一氧化氮逆转幼猪急性缺氧性肺动脉高压

目的 观察吸入不同浓度一氧化氮（ＮＯ）是否可逆转幼猪急性缺氧性肺动脉高压。方法 选用１０头上海种白猪。利用低氧建立急性缺氧性肺动脉高压模型。吸入不同浓度ＮＯ（０,１０,２０,４０,８０,１２０ｐｐｍ）。在各时相分别进行血液动力学指标、二氧化氮和高铁血红蛋白浓度监测。结果 吸入不同浓度ＮＯ可明显降低肺动脉平均压（ＭＰＡＰ）、肺循环阻力指数（ＰＶＲＩ）、跨肺压（ＴＰＧ）、体肺动脉压之比（Ｐｐ／Ｐｓ）、     

急性肺损伤

自1988年Murray等拓展了急性呼吸窘迫综合征（ARDS）的定义以来，便涉及到它的分期（急性／慢性）、基础疾病和急性肺损伤（ALI）的严重程度三个方面，并提出了一个依据胸片上肺浸润的程度、PaO2／FiO2比值、维持PaO2／FiO2所需的PEEP水平、肺顺应性4点，来评价ALI程度的评分系     

肺复张手法对急性肺损伤幼猪肺表面活性物质的影响

Objective To investigate the effect of recruitment maneuver(RM) on surfactant proteins in young piglets with acute lung injury and the possible mechanisms of lung recruitment after RM.Methods The piglet model of ALI was established by lipopolysaccharide intravenous injection,12 male piglets were randomly divided into two groups:conventional ventilation group(control group) and RM with low tidal volume group(RM group).After 8 hours of ventilation,mRNA expression of surfactant protein-A(SP-A),SP-B,SP-C,SP-D in the piglet lungs were determined by real time PCR and SP-A protein distribution was assessed by immunohistochemistry.Biochemical analyses of TP,total phospholipids(TPL),DSPC were conducted as well.SP-A levels in bronchoalveolar lavage fluid(BALF) and plasma were measured by enzymelinked immunosorbent assay(ELISA).Results As compared with control group,RM group had higher expression of SP-A,SP-B,SP-C and SP-D.SP-A average gray values of control group and RM group were 97.8±6.4 and 106.3±8.5,and there was significant difference(P＜0.01).RM group showed significant increase of TPL,DSPC and DSPC/TP.The concentration of SP-A in BALF was higher in RM group than that of the control group,however,SP-A plasma level was lower in RM group than that of the control group.Conclusion RM can increase suffactant protein expression in ALI animals,alleviate surfactant protein dysfunction and regulate the concentration of SP-A,which may improve alveolar recruitment following the RM and alleviate ventilator-induced lung injury.     

肺复张手法对急性肺损伤幼猪肺表面活性物质的影响

Objective To investigate the effect of recruitment maneuver(RM) on surfactant proteins in young piglets with acute lung injury and the possible mechanisms of lung recruitment after RM.Methods The piglet model of ALI was established by lipopolysaccharide intravenous injection,12 male piglets were randomly divided into two groups:conventional ventilation group(control group) and RM with low tidal volume group(RM group).After 8 hours of ventilation,mRNA expression of surfactant protein-A(SP-A),SP-B,SP-C,SP-D in the piglet lungs were determined by real time PCR and SP-A protein distribution was assessed by immunohistochemistry.Biochemical analyses of TP,total phospholipids(TPL),DSPC were conducted as well.SP-A levels in bronchoalveolar lavage fluid(BALF) and plasma were measured by enzymelinked immunosorbent assay(ELISA).Results As compared with control group,RM group had higher expression of SP-A,SP-B,SP-C and SP-D.SP-A average gray values of control group and RM group were 97.8±6.4 and 106.3±8.5,and there was significant difference(P＜0.01).RM group showed significant increase of TPL,DSPC and DSPC/TP.The concentration of SP-A in BALF was higher in RM group than that of the control group,however,SP-A plasma level was lower in RM group than that of the control group.Conclusion RM can increase suffactant protein expression in ALI animals,alleviate surfactant protein dysfunction and regulate the concentration of SP-A,which may improve alveolar recruitment following the RM and alleviate ventilator-induced lung injury.     

肺复张手法对急性肺损伤幼猪肺表面活性物质的影响

Objective To investigate the effect of recruitment maneuver(RM) on surfactant proteins in young piglets with acute lung injury and the possible mechanisms of lung recruitment after RM.Methods The piglet model of ALI was established by lipopolysaccharide intravenous injection,12 male piglets were randomly divided into two groups:conventional ventilation group(control group) and RM with low tidal volume group(RM group).After 8 hours of ventilation,mRNA expression of surfactant protein-A(SP-A),SP-B,SP-C,SP-D in the piglet lungs were determined by real time PCR and SP-A protein distribution was assessed by immunohistochemistry.Biochemical analyses of TP,total phospholipids(TPL),DSPC were conducted as well.SP-A levels in bronchoalveolar lavage fluid(BALF) and plasma were measured by enzymelinked immunosorbent assay(ELISA).Results As compared with control group,RM group had higher expression of SP-A,SP-B,SP-C and SP-D.SP-A average gray values of control group and RM group were 97.8±6.4 and 106.3±8.5,and there was significant difference(P＜0.01).RM group showed significant increase of TPL,DSPC and DSPC/TP.The concentration of SP-A in BALF was higher in RM group than that of the control group,however,SP-A plasma level was lower in RM group than that of the control group.Conclusion RM can increase suffactant protein expression in ALI animals,alleviate surfactant protein dysfunction and regulate the concentration of SP-A,which may improve alveolar recruitment following the RM and alleviate ventilator-induced lung injury.     

肺复张手法对急性肺损伤幼猪肺表面活性物质的影响

Objective To investigate the effect of recruitment maneuver(RM) on surfactant proteins in young piglets with acute lung injury and the possible mechanisms of lung recruitment after RM.Methods The piglet model of ALI was established by lipopolysaccharide intravenous injection,12 male piglets were randomly divided into two groups:conventional ventilation group(control group) and RM with low tidal volume group(RM group).After 8 hours of ventilation,mRNA expression of surfactant protein-A(SP-A),SP-B,SP-C,SP-D in the piglet lungs were determined by real time PCR and SP-A protein distribution was assessed by immunohistochemistry.Biochemical analyses of TP,total phospholipids(TPL),DSPC were conducted as well.SP-A levels in bronchoalveolar lavage fluid(BALF) and plasma were measured by enzymelinked immunosorbent assay(ELISA).Results As compared with control group,RM group had higher expression of SP-A,SP-B,SP-C and SP-D.SP-A average gray values of control group and RM group were 97.8±6.4 and 106.3±8.5,and there was significant difference(P＜0.01).RM group showed significant increase of TPL,DSPC and DSPC/TP.The concentration of SP-A in BALF was higher in RM group than that of the control group,however,SP-A plasma level was lower in RM group than that of the control group.Conclusion RM can increase suffactant protein expression in ALI animals,alleviate surfactant protein dysfunction and regulate the concentration of SP-A,which may improve alveolar recruitment following the RM and alleviate ventilator-induced lung injury.     

肺复张手法对急性肺损伤幼猪肺表面活性物质的影响

目的 研究肺复张手法(RM)对急性肺损伤(ALI)时肺表面活性物质表达的影响,探讨RM产生肺泡复张效应的机制.方法 健康雄性幼猪12只,静脉注射内毒素(LPS)构建ALI模型,随机分为常规潮气量通气组(对照组)和小潮气量联合肺复张手法通气组(RM组),观察8 h,酶联免疫吸附法检测血浆、支气管肺泡灌洗液(BALF)中的肺表面活性蛋白(SP)-A浓度,分析BALF中的磷脂成分[总磷脂(TPL)、活性磷脂(DSPC)及总蛋白(TP)],并采用RT-PCR和免疫组织化学染色测定肺组织中SP-A、SP-B、SP-C、SP-D的mRNA及SP-A的蛋白表达水平.结果 与对照组相比,RM组中SP-A、SP-B、SP-C、SP-D的mRNA水平明显升高,对照组与RM组SP-A平均灰度值为97.8±6.4与106.3±8.5,差异有非常显著性(P＜0.01),并且采用RM后BALF中DSPC/TP、DSPC/TPL比例升高,血浆中SP-A浓度降低,而BALF中的SP-A浓度明显上升.结论 肺复张手法可以改善ALl幼猪肺表面活性物质相关蛋白的合成及肺部表达,增加肺表面活性物质的活性,调节SP-A的浓度,有助于促进肺复张后的肺泡开放,并减轻呼吸机相关肺损伤.     

肺复张手法对急性肺损伤幼猪肺表面活性物质的影响

Objective To investigate the effect of recruitment maneuver(RM) on surfactant proteins in young piglets with acute lung injury and the possible mechanisms of lung recruitment after RM.Methods The piglet model of ALI was established by lipopolysaccharide intravenous injection,12 male piglets were randomly divided into two groups:conventional ventilation group(control group) and RM with low tidal volume group(RM group).After 8 hours of ventilation,mRNA expression of surfactant protein-A(SP-A),SP-B,SP-C,SP-D in the piglet lungs were determined by real time PCR and SP-A protein distribution was assessed by immunohistochemistry.Biochemical analyses of TP,total phospholipids(TPL),DSPC were conducted as well.SP-A levels in bronchoalveolar lavage fluid(BALF) and plasma were measured by enzymelinked immunosorbent assay(ELISA).Results As compared with control group,RM group had higher expression of SP-A,SP-B,SP-C and SP-D.SP-A average gray values of control group and RM group were 97.8±6.4 and 106.3±8.5,and there was significant difference(P＜0.01).RM group showed significant increase of TPL,DSPC and DSPC/TP.The concentration of SP-A in BALF was higher in RM group than that of the control group,however,SP-A plasma level was lower in RM group than that of the control group.Conclusion RM can increase suffactant protein expression in ALI animals,alleviate surfactant protein dysfunction and regulate the concentration of SP-A,which may improve alveolar recruitment following the RM and alleviate ventilator-induced lung injury.     

肺复张手法对急性肺损伤幼猪肺表面活性物质的影响

Objective To investigate the effect of recruitment maneuver(RM) on surfactant proteins in young piglets with acute lung injury and the possible mechanisms of lung recruitment after RM.Methods The piglet model of ALI was established by lipopolysaccharide intravenous injection,12 male piglets were randomly divided into two groups:conventional ventilation group(control group) and RM with low tidal volume group(RM group).After 8 hours of ventilation,mRNA expression of surfactant protein-A(SP-A),SP-B,SP-C,SP-D in the piglet lungs were determined by real time PCR and SP-A protein distribution was assessed by immunohistochemistry.Biochemical analyses of TP,total phospholipids(TPL),DSPC were conducted as well.SP-A levels in bronchoalveolar lavage fluid(BALF) and plasma were measured by enzymelinked immunosorbent assay(ELISA).Results As compared with control group,RM group had higher expression of SP-A,SP-B,SP-C and SP-D.SP-A average gray values of control group and RM group were 97.8±6.4 and 106.3±8.5,and there was significant difference(P＜0.01).RM group showed significant increase of TPL,DSPC and DSPC/TP.The concentration of SP-A in BALF was higher in RM group than that of the control group,however,SP-A plasma level was lower in RM group than that of the control group.Conclusion RM can increase suffactant protein expression in ALI animals,alleviate surfactant protein dysfunction and regulate the concentration of SP-A,which may improve alveolar recruitment following the RM and alleviate ventilator-induced lung injury.     

肺复张手法对急性肺损伤幼猪肺表面活性物质的影响

Objective To investigate the effect of recruitment maneuver(RM) on surfactant proteins in young piglets with acute lung injury and the possible mechanisms of lung recruitment after RM.Methods The piglet model of ALI was established by lipopolysaccharide intravenous injection,12 male piglets were randomly divided into two groups:conventional ventilation group(control group) and RM with low tidal volume group(RM group).After 8 hours of ventilation,mRNA expression of surfactant protein-A(SP-A),SP-B,SP-C,SP-D in the piglet lungs were determined by real time PCR and SP-A protein distribution was assessed by immunohistochemistry.Biochemical analyses of TP,total phospholipids(TPL),DSPC were conducted as well.SP-A levels in bronchoalveolar lavage fluid(BALF) and plasma were measured by enzymelinked immunosorbent assay(ELISA).Results As compared with control group,RM group had higher expression of SP-A,SP-B,SP-C and SP-D.SP-A average gray values of control group and RM group were 97.8±6.4 and 106.3±8.5,and there was significant difference(P＜0.01).RM group showed significant increase of TPL,DSPC and DSPC/TP.The concentration of SP-A in BALF was higher in RM group than that of the control group,however,SP-A plasma level was lower in RM group than that of the control group.Conclusion RM can increase suffactant protein expression in ALI animals,alleviate surfactant protein dysfunction and regulate the concentration of SP-A,which may improve alveolar recruitment following the RM and alleviate ventilator-induced lung injury.     

肺复张手法对急性肺损伤幼猪肺表面活性物质的影响

Objective To investigate the effect of recruitment maneuver(RM) on surfactant proteins in young piglets with acute lung injury and the possible mechanisms of lung recruitment after RM.Methods The piglet model of ALI was established by lipopolysaccharide intravenous injection,12 male piglets were randomly divided into two groups:conventional ventilation group(control group) and RM with low tidal volume group(RM group).After 8 hours of ventilation,mRNA expression of surfactant protein-A(SP-A),SP-B,SP-C,SP-D in the piglet lungs were determined by real time PCR and SP-A protein distribution was assessed by immunohistochemistry.Biochemical analyses of TP,total phospholipids(TPL),DSPC were conducted as well.SP-A levels in bronchoalveolar lavage fluid(BALF) and plasma were measured by enzymelinked immunosorbent assay(ELISA).Results As compared with control group,RM group had higher expression of SP-A,SP-B,SP-C and SP-D.SP-A average gray values of control group and RM group were 97.8±6.4 and 106.3±8.5,and there was significant difference(P＜0.01).RM group showed significant increase of TPL,DSPC and DSPC/TP.The concentration of SP-A in BALF was higher in RM group than that of the control group,however,SP-A plasma level was lower in RM group than that of the control group.Conclusion RM can increase suffactant protein expression in ALI animals,alleviate surfactant protein dysfunction and regulate the concentration of SP-A,which may improve alveolar recruitment following the RM and alleviate ventilator-induced lung injury.     

肺复张手法对急性肺损伤幼猪肺表面活性物质的影响

Objective To investigate the effect of recruitment maneuver(RM) on surfactant proteins in young piglets with acute lung injury and the possible mechanisms of lung recruitment after RM.Methods The piglet model of ALI was established by lipopolysaccharide intravenous injection,12 male piglets were randomly divided into two groups:conventional ventilation group(control group) and RM with low tidal volume group(RM group).After 8 hours of ventilation,mRNA expression of surfactant protein-A(SP-A),SP-B,SP-C,SP-D in the piglet lungs were determined by real time PCR and SP-A protein distribution was assessed by immunohistochemistry.Biochemical analyses of TP,total phospholipids(TPL),DSPC were conducted as well.SP-A levels in bronchoalveolar lavage fluid(BALF) and plasma were measured by enzymelinked immunosorbent assay(ELISA).Results As compared with control group,RM group had higher expression of SP-A,SP-B,SP-C and SP-D.SP-A average gray values of control group and RM group were 97.8±6.4 and 106.3±8.5,and there was significant difference(P＜0.01).RM group showed significant increase of TPL,DSPC and DSPC/TP.The concentration of SP-A in BALF was higher in RM group than that of the control group,however,SP-A plasma level was lower in RM group than that of the control group.Conclusion RM can increase suffactant protein expression in ALI animals,alleviate surfactant protein dysfunction and regulate the concentration of SP-A,which may improve alveolar recruitment following the RM and alleviate ventilator-induced lung injury.     

肺复张手法对急性肺损伤幼猪肺表面活性物质的影响

Objective To investigate the effect of recruitment maneuver(RM) on surfactant proteins in young piglets with acute lung injury and the possible mechanisms of lung recruitment after RM.Methods The piglet model of ALI was established by lipopolysaccharide intravenous injection,12 male piglets were randomly divided into two groups:conventional ventilation group(control group) and RM with low tidal volume group(RM group).After 8 hours of ventilation,mRNA expression of surfactant protein-A(SP-A),SP-B,SP-C,SP-D in the piglet lungs were determined by real time PCR and SP-A protein distribution was assessed by immunohistochemistry.Biochemical analyses of TP,total phospholipids(TPL),DSPC were conducted as well.SP-A levels in bronchoalveolar lavage fluid(BALF) and plasma were measured by enzymelinked immunosorbent assay(ELISA).Results As compared with control group,RM group had higher expression of SP-A,SP-B,SP-C and SP-D.SP-A average gray values of control group and RM group were 97.8±6.4 and 106.3±8.5,and there was significant difference(P＜0.01).RM group showed significant increase of TPL,DSPC and DSPC/TP.The concentration of SP-A in BALF was higher in RM group than that of the control group,however,SP-A plasma level was lower in RM group than that of the control group.Conclusion RM can increase suffactant protein expression in ALI animals,alleviate surfactant protein dysfunction and regulate the concentration of SP-A,which may improve alveolar recruitment following the RM and alleviate ventilator-induced lung injury.