恶性肿瘤患者手术前后血清IL—2／sIL—2R水平检测

目的：研究机体免疫功能的变化与恶性肿瘤发生及预后的关系。方法：采用ＭＴＴ比色法及双抗体夹心法对４２例胃癌，３５例乳腺癌，２８例大肠癌手术前后ＩＬ－２及ｓＩＬ－２Ｒ水平进行检测，并以健康献血员对照。结果：３组恶性肿瘤患者是ＩＬ－２活性明显低于正常人，血清ｓＩＬ－２Ｒ水平明显高于正常人。术后肿瘤复发患者ＩＬ－２／ｓＩＬ－２Ｒ水平持续异常，未复发患者ＩＬ－２／ｓＩＬ－２Ｒ水平恢复正常，结论：机体免疫功能     

恶性肿瘤放疗后血清可溶性白细胞介素2受体含量变化的临床意义

目的 探讨血清可溶性白细胞介素－２受体水平与恶性肿瘤患者的病期及疗效的关系。方法 采用双抗体夹心ＥＬＩＳＡ法检测１５９例恶性肿瘤患者放疗前后血清ｓＩＬ－２Ｒ水平。结果 恶性肿瘤患者放疗前血清ｓＩＬ－２Ｒ水平明显高于正常对照组；放疗后血清ｓＩＬ－２Ｒ水平明显低于治疗前，晚期患者不论是放疗前或放疗后ｓＩＬ－２Ｒ水平均明显高于早期患者；     

肿瘤患者血清sIL-2R水平变化的临床意义

目的：观察肿瘤患者血清可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）水平的变化及临床意义。方法：采用双抗体夹心ＥＬＩＳＡ法对４０例正常人、３０例良性肿瘤及１８０例恶性肿瘤患者的血清ｓＩＬ－２Ｒ水平进行测定。结果：①正常人和良性肿瘤者血清ｓＩＬ－２Ｒ水平较低,恶性肿瘤患者明显升高,与正常人及良性肿瘤患者比较均有极显著性差异（ｐ〈０．００１）;②ｓＩＬ－２Ｒ水平与临床分期及病情严重程度有关,处于Ⅲ、Ⅳ期的肿瘤     

食管癌患者血清可溶性白细胞介素2受体水平观察及临床意义

本文采用双抗体夹心ＥＬＩＳＡ法对３１例食管患者的血清可溶性白细胞介素２受体，ｓＩＬ－２Ｒ进行测定观察，结果发现：食管癌患者的血清ｓＩＬ－２Ｒ水平显高于正常对照组（Ｐ〈０．０１），随着临床分期的增加，ｓＩＬ－２Ｒ水平亦增加，手术前患者血清ｓＩＬ－２Ｒ较手术后为高（Ｐ〈０．０１），所以食管癌患者血清ｓＩＬ－２Ｒ水平可以反映疾病的发生与发展，可做为判断预后，监测复发和转移的客观指标。     

血液病病人血清可溶性白细胞介素2受体的研究

用双抗体夹心酶联免疫吸附法检测了１６２例血液病病人血清可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）水平。结果表明，急、慢性白血病（ＡＬ，ＣＬ）、何杰金氏淋巴瘤和骨髓增生异常综合症（ＭＤＳ）组血清ｓＩＬ－２Ｒ水平显著高于缺铁性贫血、慢性再生障碍性贫血及正常对照组（Ｐ＜０．０１）。而且血清ｓＩＬ－２Ｒ水平与病情变化有关，完全缓解（ＣＲ）的ＡＬ病人血清ｓＩＬ－２Ｒ水平较治疗前明显降低，其水平高低与维持缓解的时间有关。ＭＤＳ亚型中预后差的转化中原始细胞过多的难治性贫血病人血清ｓＩＬ－２Ｒ水平明显高于难治性贫血及难治性贫血伴环形铁粒幼红细胞者，而与急性非淋巴细胞性白血病水平无显著差异（Ｐ＞０．０５）。提示高水平的血清ｓＩＬ－２Ｒ与造血系统恶性肿瘤肿及瘤细胞负荷有密切关系，动态观察其血清ｓＩＬ－２Ｒ水平的变化有助于临床监护、疗效观察及预后估计，亦对探讨免疫功能异常与恶性血液病的发生、发展的关系有重要意义。     

检测血清可溶性白细胞介素－2受体在恶性肿瘤诊断中的应用

应用双抗夹心ＥＬＩＳＡ法检测１１１例各类恶性肿瘤患者血清中可溶性白细胞介素－２受体（ｓＩＬ－２Ｒ）水平。结果表明，恶性肿瘤患者ｓＩＬ－２Ｒ水平明显高于正常人（Ｐ＜０．０１），而肺癌、乳腺癌、肝癌、胃癌、膀胱癌、卵巢癌及宫颈癌之间无明显差异（Ｐ＞０．０５）。在４５例肺癌中，有转移者的ｓＩＬ－２Ｒ水平明显高于无转移者（Ｐ＜０．０１）。ｓＩＬ－２Ｒ对恶性肿瘤的诊断，治疗和预后的判断具有重要意义。     

肾炎患者血清sIL—2R水平变化研究

应用双抗体夹心ＥＬＩＳＡ检测急性肾炎、慢性肾炎、慢性肾功能不全患者共４８例血清ｓＩＬ－２Ｒ水平。结果显示，３组患者血清患者血清ｓＩＬ－２Ｒ水平均显著高于正常对照，提示肾炎患者体内存在着淋巴细胞激活状态；对部分患者血清ｓＩＬ－２Ｒ水平进行动态观察发现，肾炎患者实现 情好转或痊愈，血清ｓＩＬ－２Ｒ的水平下降或恢复正常。     

肺癌患者化疗前后血清可溶性白细胞介素2受体水平测定

采用双抗体夹心间接ＥＩＡ法对１６例肺癌患者化疗前后血清可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）含量进行了测定。结果显示：肺癌患者化疗前血清ｓＩＬ－２Ｒ水平显著高于正常对照（Ｐ〈０．０１）；化疗后血清ｓＩＬ－２Ｒ水平较化疗前显著降低（Ｐ〈０．０１）。表明肺癌患者血清ｓＩＬ－２Ｒ水平与其肿瘤的消长密切相关，血清ｓＩＬ－２Ｒ有可能作为疗效和判断预后的一个重要监测指标。     

改良张力带治疗髌骨骨折所致并发症的原因分析

观察平消胶囊对放疗的恶性肿瘤病人血清ｓＩＬ－２Ｒ水平的影响。方法；采用ＥＬＩＳＡ双抗体夹心法。结果：恶性肿瘤患者血清ｓＩＬ－２Ｒ含量高于正常组，放疗后ｓＩＬ－２Ｒ含量高于放疗前，但Ｐ〉０．０５。放疗与平消胶囊合并应用组放疗后ｓＩＬ－２Ｒ含量低于放疗前。     

原发性癫痫患者血清可溶性白细胞介素2受体水平的观察

采用双本夹心ＥＬＩＳＡ法对４３例原发性癫痫患者和５６例正常对照血清可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）水平进行检测。结果显示癫痫组血清ｓＩＬ－２Ｒ水平显著高于下沉对照组，血清ｓＩＬ－２Ｒ民癫痫患者的年龄，性别、病程无关，抗癫痫药物对癫痫患者血清ｓＩＬ－２Ｒ水平无明显影响。结果提示癫痫患者存在细胞免疫功能异常，Ｔ淋巴细胞处于活化状态。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.